Effects of drought stress on antioxidant enzyme, photosynthetic pigment and flavonoid pathway in two desert shrubs   总被引：1，自引：0，他引：1  

YuBing Liu  MeiLing Liu  Bo Cao 《寒旱区科学》2011,3(4):0332-0338

Antioxidant enzyme activity, photosynthetic pigment content, and free malondialdehyde (MDA), as well as flavonoid content and the key enzyme activity in the flavonoid pathway were determined in two desert shrubs, Caryopteris mongolica Bunge and Reaumuria soongorica (Pall.) Maxim. under drought stress. The free MDA content was enhanced during the experimental period, which may be an indicator of oxidative stress. Superoxide dismutase (SOD), peroxidase (POD), and ascorbate peroxidase (APX) activities in C. mongholica showed a significant increase during the experiment, but catalase (CAT) activity was slightly decreased. On the other hand, POD and APX activities showed a significant increase and SOD and CAT activity data had no significant changes in R. soongorica. APX, SOD, and CAT activities were higher in R. soongorica than in C. mongholica, but MDA content was lower, indicating that the lower values of MDA were attributed to higher activities of antioxidant enzyme in R. soongorica. Chlorophyll content decreased significantly in the two shrubs during the experiment, which indicated that there was a photoprotection mechanism through reducing light absorbance by decreasing pigments content. Caretonoids content increased in C. mongholica and decreased in R. soongorica. The ratio of Chla/Chlb decreased significantly but caretonoids/Chl revealed a significant increase in the two shrubs, which could be explained as no decrease of peripheral light-harvesting complexes and a higher tolerance to drought. Total flavonoid content and the activities of phenylalanine ammonialyase (PAL) and chalcone isomerase (CHI) showed different changes between C. mongholica and R. soongorica after treatment. These values decreased in R. soongorica and increased in C. mongholica except for PAL activity. However, anthocyanin content increased in the two shrubs, indicating that there was a different regulation response in the flavonoid pathway in the two shrubs under drought stress, and anthocyanin should be an important antioxidant both in the shrubs. Our results demonstrated the different responses of antioxidant defense and drought tolerance ability between the two shrubs.  相似文献   

杭州湾湿地土壤酶活性分布特征及其与活性有机碳组分的关系   总被引：1，自引：0，他引：1  

杨文英  邵学新  梁威  吴明 《湿地科学与管理》2011,7(2):54-58

选取了杭州湾滨海湿地芦苇Phragmites australis湿地、互花米草Spartina alterniflora湿地、海三棱藨草Scirpus mariqueter湿地以及光滩湿地,研究了土壤脲酶、蔗糖酶、β-葡萄糖苷酶、磷酸酶活性及土壤溶解性碳(DOC)、土壤微生物量碳(MBC)在0～20cm土层的分布特征及其内在相关性,探讨酶活性与土壤有机碳及其组分的关系。结果表明,表层(0～5cm)土壤的酶活性和有机碳含量最高,随着土壤深度的增加,土壤酶活性和土壤有机碳含量呈下降趋势。相关分析表明,土壤碱性磷酸酶、蔗糖酶与总有机碳呈显著正相关,土壤脲酶与土壤溶解性有机碳呈显著正相关,蔗糖酶、β-葡萄糖苷酶以及碱性磷酸酶活性与土壤微生物量碳或溶解性碳呈负相关,表明不同的土壤酶对湿地土壤有机碳库组成的贡献不同。  相似文献   

低值鱼蛋白多肽-铁(Ⅱ)螯合物的酶解制备及其抗氧化、抗菌活性   总被引：1，自引：0，他引：1  

邓尚贵  杨燊  秦小明 《广东海洋大学学报》2006,26(4):54-58

以南海低值鱼蛋白为原料,采用木瓜蛋白酶和风味酶的复合酶水解得到多肽水解物(HP),并制备多肽-铁(Ⅱ)螯合物,对螯合工艺及螯合产物的结果及抗氧化和抗菌功能特性进行了初步研究。结果表明:1)水解度为5%的多肽与铁(Ⅱ)螯合效果最佳,螯合率为94.15%;2)化学分析及红外光谱表明活性肽与亚铁离子螯合并形成稳定结构的螯合物;(3)经过50%乙醇(体积分数)沉淀的螯合铁具有明显的抗氧化活性,其抗氧化作用相当于维生素E的90%,经过80%乙醇(体积分数)沉淀的螯合物具有显著的抗枯草牙孢杆菌和金黄色葡萄球菌活性,其抑菌圈均直径为13mm。  相似文献   

Changes in the Contents of ATP and Its Related Breakdown Compounds in Various Tissues of Oyster During Frozen Storage     

WANG Qi XUE Changhu LI Zhaojie FU Xueyan XU Jie XUE Yong 《中国海洋大学学报(英文版)》2007,6(4):407-412

The changes in the contents of adenosine 5’-triphosphate (ATP) and its related breakdown compounds were investigated in the adductor muscle, mantle, gill and body trunk of oyster (Crassostrea gigas) during frozen storage at -20℃ and -30℃ and compared with that of the fresh oyster. The investigation was performed using an HPLC system. Different extents of ATP decomposi- tion were found in various tissues frozen at the two temperatures. The K, K’ and A.E.C values were calculated as the chemical fresh- ness indices. Considering the results of sensory evaluation, the A.E.C. value in body trunk at -20℃ and -30℃ could be used as the best freshness index for frozen oyster.  相似文献   

三氯异氰尿酸对剑尾鱼(Xiphophorus helleri)毒性及其抗氧化酶影响   总被引：3，自引：0，他引：3       下载免费PDF全文

聂湘平  王翔  李凯彬  吴淑勤 《海洋与湖沼》2008,39(5):494-498

采用生物毒性测试与评价方法对常用消毒剂药物三氯异氰尿酸（Trichloroisocyanuric acid，TCCA）对剑尾鱼的急性毒性及其Ⅰ、Ⅱ相代谢酶活性影响进行了研究。结果表明，TCCA对剑尾鱼的96hLG0为2．15mg／L。TCCA对剑尾鱼肝脏还原型谷光苷肽（GSH）、谷胱苷肽硫转酶（GST）和7。乙氧基异吩恶唑酮-脱乙基酶（EROD）都存在诱导作用。雌雄个体在GST和EROD的诱导响应时间有显著差异性，其中雄性个体GST对TCCA暴露响应比雌性个体敏感。  相似文献   

青鳞鱼蛋白复合酶控制水解物功能特性的研究   总被引：1，自引：0，他引：1  

邓尚贵  彭志英  杨萍  常志娟 《广东海洋大学学报》2005,25(3):13-18

研究了青鳞鱼蛋白复合酶控制水解产物的功能特性。结果表明,复合酶控制水解能够显著地改善水解物的溶解性、乳化活力及起泡性能。水解物在pH 2.0~10.0范围内其NSI为89.3%~98.5%,且在酸性范围NSI高于碱性范围;在酸性pH内水解物的乳化活力都高于112 m2/g,随着DH的增加,最大乳化活力出现的pH范围更宽广,但水解却降低了乳化稳定性。蛋白质质量分数为1%~5%时,FP随质量分数的增加而增加;泡沫形成后,未水解的蛋白质具有最高的稳定性,DH为8.4%的水解物具有最高的稳定性。  相似文献   

An Effective Method of Prompting Juvenile Rainbow Trout (Oncorhynchus mykiss) to Cope with Heat Stress     

JIANG Xuyang  DONG Shuanglin  ZHOU Yangen  GAO Qinfeng  PAN Zhe 《中国海洋大学学报(英文版)》2020,19(1):216-224

  相似文献   

Colonization and Probiotic Effect of Metschnikowia sp. C14 in the Intestine of Juvenile Sea Cucumber,Apostichopus japonicus     

LI Ming  BAO Pengyun  SONG Jian  DING Jianfeng  LIU Yubin  MA Yuexin 《中国海洋大学学报(英文版)》2020,19(1):225-231

Viable cell count was used to determine whether Metschnikowia sp.C14 can colonize the intestine of juvenile sea cucumber Apostichopus japonicus.Sea cucumber individuals were divided into two groups,which were fed the control diet for 38 days or the C14-supplemented diet at 105 cells g−1 diet for 28 days,then the control diet from day 29 to day 38.The number of C14 cells in the intestine of sea cucumber fed the C14-supplemented diet significantly increased from day 7 to day 28,and decreased from day 29 to day 38.Sea cucumber fed with the diet containing C14 showed a significant increase in trypsin activity and lipase activity from day 21 to day 33 compared with the control.Feeding C14 significantly improved the phagocytic activity and respiratory burst in coelomocytes from day 21 to day 35 and from day 14 to day 38,respectively.In addition,there was an obvious enhancement in lysozyme activity(from day 21 to day 38 or day 33),phenoloxidase activity(from day 21 to day 28)and total nitric oxide synthase activity(from day 14 to day 38)in coelomic fluid supernatant and/or coelomocyte cell lysate supernatant compared with the control.There were significant positive correlations between the number of C14 cells colonizing the intestine and trypsin activity of the intestine,lysozyme activity of the coelomic fluid supernatant and coelomocyte lysate supernatant from sea cucumber.These data suggested that the number of C14 cells should be maintained at 105 cfu(colony-forming units)g−1 intestine material for the maximum benefit.  相似文献   

南极冰穹A低氧复合寒冷环境对考察队员儿茶酚胺含量和心肌酶的影响          下载免费PDF全文

熊艳蕾 彭毛加措 陈楠宋敏涛  徐成丽 《极地研究》2016,28(3):311-316

南极冰穹A地区气候条件极为恶劣,通过探讨冰穹A低氧复合寒冷环境对考察队员儿茶酚胺激素和心肌酶水平的影响,为冰穹A考察队员医学防治提供依据。分别在上海出发、冰穹A考察和返回上海时,采集第29次中国南极考察昆仑站冰盖队25名男队员外周静脉血样,检测血中去甲肾上腺素(NE)、肾上腺素(E)、多巴胺(DA)、高敏感性C反应蛋白(hs CRP)、肌酸激酶MB(CK-MB)和肌钙蛋白I(c Tn I)水平。队员E和CK-MB水平在冰穹A虽增高但与出发时没有显著性差异,E水平在返回上海后显著降低(P0.01);DA水平在冰穹A和返回上海后均显著增高(P0.05)。与出发(P0.01)和冰穹A(P0.01)数据相比,CK-MB水平在返回上海后均显著降低。肌钙蛋白I(c Tn I)水平在冰穹A和返回上海后均没有发生显著变化,均在正常范围内(0.04 ng·m L-1),hs CRP和NE水平也没有显著性改变。Pearson相关性分析表明,CK-MB水平与E水平呈显著正相关(r=0.285*,P0.05)。结果表明,在冰穹A低氧复合高寒环境下冰盖队员心肌出现了适应性代偿,返回上海后没有出现病理性损伤,心肌酶水平变化与儿茶酚胺类激素水平存在相关性,今后仍需积累多次昆仑站考察队的数据资料,深入探讨潜在的调节机制。  相似文献   

饲料蛋白质水平对拟目乌贼生长前期的生长性能、肌肉成分和酶活的影响     

汪元  蒋霞敏  乐可鑫  韩庆喜  彭瑞冰  梁晶晶  王鹏帅 《海洋科学》2016,40(3):87-94

以鱼粉、豆粕和明胶为主要蛋白源配制成蛋白质水平分别为31.5%、35.0%、38.5%、42.0%、45.5%、49.0%的试验饲料。选取初始均重(3.99±0.08)g的拟目乌贼(Sepia lycidas)540只,随机分成6组,每组3个重复,每个重复30只。结果表明:拟目乌贼的生长性能指标(增重率、特定生长率、成活率)均在饲料蛋白质水平为45.91%时达到最佳,除与蛋白质水平为49.72%时差异不显著(P0.05)外,与其他各组均有显著差异(P0.05)。饲料系数随蛋白质水平的升高呈先降后升的趋势,在饲料蛋白质水平为45.91%时达到最低,显著低于除49.72%组外的其他各组;蛋白质效率随着饲料中蛋白质水平的升高而显著下降(P0.05)。饲料蛋白质水平对肥满度与肝体比影响不显著(P0.05)。胃蛋白酶、胰蛋白酶、肠道脂肪酶和肝脏超氧化物歧化酶活性,随饲料蛋白质水平的增加呈先升后降的趋势,均在饲料蛋白质水平为45.91%时达到最高,胃蛋白酶和胰蛋白酶活性除与蛋白质水平为42.22%时差异不显著(P0.05)外,与其他各组均有显著差异(P0.05);脂肪酶和肝脏超氧化物歧化酶活性与其他各组均有显著差异(P0.05),而肠道淀粉酶活性则相反。肝脏谷丙转氨酶和谷草转氨酶活性随饲料蛋白质水平的增加呈先升后稳定的趋势,均在饲料蛋白质水平为49.72%时达到最大,显著高于除45.91%组外的其他各组(P0.05);而饲料蛋白质水平对碱性磷酸酶活性影响不显著(P0.05)。乌贼肌肉粗蛋白质在饲料蛋白质水平45.91%时含量最高,显著高于除49.72%组外的其他各组(P0.05);粗脂肪含量最低,显著低于其他各组(P0.05);而肌肉水分和粗灰分含量不受饲料蛋白质水平的影响(P0.05)。拟目乌贼生长前期(4~10 g)配合饲料适宜的蛋白质水平为45.91%。  相似文献