Microscopic structures of Japanese lamprey peripheral blood cells and culture of lymphocyte-like cells in vitro     

《海洋科学》2012,36(1)

以日本七鳃鳗（Lampetrajaponica）为研究对象,观察其外周血细胞显微结构和探讨类淋巴细胞原代培养条件。采用Wright氏和Giemsa氏染色法对日本七鳃鳗外周血液有形成分进行显微观察,可鉴别出红细胞、淋巴细胞、中性粒细胞、单核细胞、嗜酸性粒细胞：未发现嗜碱性粒细胞。通过Ficoll密度梯度离心法和流式细胞仪分选获得高纯度的七鳃鳗类淋巴细胞。经条件优化后确定最适培养液L15＋20％FBS（5％七鳃鳗血清）＋0．46％NaCl,在18℃,pH6．8～7．0的条件下对日本七鳃鳗类淋巴细胞进行体外培养,大多数类淋巴细胞半贴壁生长,细胞状态较好,最长可以存活近一个月。类淋巴细胞的原代培养为日本七鳃鳗细胞体外培养的深入研究提供了实验依据。  相似文献   

褐牙鲆鳃氯细胞的超微结构研究   总被引：4，自引：0，他引：4  

姜明  范瑞青  刘云  孙长青  谢嘉琳 《海洋科学》2003,27(10):1-5

利用透射电镜技术，研究了褐牙鲆(Paralichthys olrvaceus)鳃氯细胞的超微结构特征。研究表明，褐牙鲆鳃氯细胞主要分布于鳃小叶基部，具有微细小管系统和分泌腔。有丰富的线粒体，细胞核基位。根据氯细胞结构的不同，将其分为4种类型：I型氯细胞、Ⅱ型氯细胞、Ⅲ型氯细胞和Ⅳ型氯细胞。I型氯细胞胞体较大，细胞基质密度较低。微细小管系统发达呈细管状；Ⅱ型氯细胞外形不规则。胞体较大，细胞基质密度较高，微细小管系统主要呈粗网状；Ⅲ型氯细胞外形不规则，细胞基质具有很高的电子密度，微细小管系统不发达，胞内存在大量线粒体，分泌腔小；Ⅳ型氯细胞为扁平状，细胞体积较小，具有较大的分泌腔，但微细小管系统不发达。褐牙鲆鳃氯细胞系统是主要的渗透压调节系统。  相似文献   

Effects of basic fibroblast growth factor and insulin-like growth factor on cultured cartilage cells from skate Raja porasa     

Fan Tingjun  Jin Lingyun  Wang Xiaofeng 《中国海洋湖沼学报》2003,21(4):305-311

Effects of basic fibroblast growth factor (bFGF) and insulin-like growth factor II (IGF-II) on cartilage cells from proboscis of skate,Raja porasa Günther, were investigated in this study. The cartilage cells were cultured in 20% FBS-supplemented MEM medium at 24°C. Twelve hours after culture initiation, the cartilage cells were treated with bFGF and IGF-II at different concentration combinations. It was found that 20 ng/ml of bFGF or 80 ng/ml of IGF-II was enough to have obvious stimulating effect on the growth and division of skate cartilage cells. Test of bFGF and IGF-II together, revealed that 20 ng/ml of bFGF and 80 ng/ml of IGF-II together had the best stimulating effect on the growth and division of skate cartilage cells. The cartilage cells cultured could form a monolayer at day 7. This work was partially supported and funded by the China Education Committee as the “Imbursement Project for Studied Abroad Returnees”.  相似文献   

Analysis of mesoscale convective systems in Catalonia using meteorological radar for the period 1996–2000     

Tomeu Rigo  Maria-Carmen Llasat   《Atmospheric Research》2007,83(2-4):458-472

The aim of this paper is to show a climatology of Mesoscale Convective Systems (MCS) in the NE of the Iberian Peninsula, on the basis of meteorological radar observations. Special attention was paid to those cases that have produced heavy rainfalls during the period 1996–2000. Identification of the MCS was undertaken using two procedures. Firstly, the precipitation structures at the lowest level were recognised by means of a 2D algorithm that distinguishes between convective and non-convective contribution. Secondly, the convective cells were identified using a 3D procedure quite similar to the SCIT (Storm Cell Identification and Tracking) algorithm that looks for the reflectivity cores in each radar volume. Finally, the convective cells (3D) were associated with the 2D structures (convective rainfall areas), in order to characterize the complete MCS. Once this methodology was presented the paper offers a proposal for classifying the precipitation systems, and particularly the MCS. 57 MCS structures were classified: 49% of them were identified as linearly well-organised systems, called TS (39%), LS (18%) and NS (43%). In addition to the classification, the following items were analysed for each MCS found: duration, season, time of day, area affected and direction of movement, and main radar parameters related with convection. The average features of those MCS show an area of about 25000 km², Z_max values of 47 dBz, an echotop of 12 km, the maximum frequency at 12 UTC and early afternoon and a displacement towards E-NE. The study was completed by analysing the field at surface, the presence of a mesoscale low near the system and the quasi-stationary features of three cases related with heavy rainfalls. Maximum rainfall (more then 200 mm in 6 h) was related with the presence of a cyclone in combination with the production of a convective train effect.  相似文献   

Extraction of plasmid-like DNA and high-quality total DNA from Porphyra yezoensis   总被引：3，自引：0，他引：3  

Guo Baotai  Dai Jixun  Shen Songdong  Bi Yuping  Shan Lei  Li Guangcun 《海洋学报(英文版)》2000,19(2):83-88

I~ODWrIONExtraction of DNA from Porphyra po~nsis is very difficult due tO its high content ofpolysaccharides. TO avoid the interference Of POlysaccharides, HOng et al. (1992) used LiCI tosoften Porphyra thalli cells to release DNA directly without lysing the thalli, hoWever, the procedure is problematic in the isolation of high-quality DNA. Kitade et al. (1996) established a complicated procedure which includes the grindiflg of material in liquid nitrogen, lysing of material inextra…  相似文献   

鳗弧菌对魁蚶血细胞形态及免疫功能的影响   总被引：3，自引：1，他引：2  

周丽青  杨爱国  王清印  吕振明  刘志鸿  田吉腾  吴彪 《海洋与湖沼》2014,45(3):536-541

本研究采用快速瑞氏-吉姆萨染色法观察鳗弧菌(Vibrio anguillarum)刺激后魁蚶(Scapharca broughtonii)几种血细胞的形态变化,研究不同种类血细胞的免疫功能。魁蚶血淋巴液中红细胞、白细胞和血栓细胞经免疫刺激后均发生形态改变,属于免疫防御的应激反应。经鳗弧菌免疫刺激后,红细胞无丝分裂现象增多,并参与病原菌的吞噬作用;白细胞变形运动、吞噬和融合现象普遍,细胞表面具有粘性,参与血栓的形成;血栓细胞主要表现出粘连和凝血的特性,偶有吞噬病原菌的现象;研究中检测了2种常规抗凝剂的抗凝效果,结果表明,2种抗凝剂分别改变了血栓细胞和白细胞的表面粘连特性,从而阻止它们参与凝血,因而都具有抗凝效果。本研究结果表明,魁蚶血细胞分工明确,能共同防御病原菌的入侵,细胞免疫在魁蚶血淋巴液抗菌免疫中起极其重要的作用,血细胞的形态变化和免疫功能分析可为后期研究魁蚶抗菌机制和筛选抗凝剂奠定基础。  相似文献   

Size of anal papillae in chironomids: Does it indicate their salinity stress?     

Ben J. Kefford  Kasturi Reddy-LopataColin Clay  Tarah HagenOrsolya Parkanyi  Dayanthi Nugegoda 《Limnologica》2011,41(2):96-106

Salinity of inland waters is affected by a range of human activities and is regarded as a major environmental contaminant in many parts of the world. Changes in salinity are well known to be associated with changes in macroinvertebrate communities of flowing waters. However, as many environmental factors co-vary with salinity, it is not known whether, and if so how, salinity causes communities to change. Being able to measure the osmoregulatory stress that individual stream macroinvertebrates are experiencing would be useful to understand if and how salinity affects their populations and thus communities. Additionally, inferring salinity stress in individual invertebrates could provide a valuable biomonitoring tool to detect the initial effects of salinity before major ecological changes have occurred. Osmoregulation in larval Chironomidae (Diptera) takes place in the anal papillae and their size is believed to be associated with osmoregulatory stress. In two laboratory experiments and a field survey in southern Victoria, Australia, we determine if the size of the anal papillae of larva chironomids is a useful biomarker of salinity stress. Experiments with Chironomus oppositus showed that the surface area of the anal papillae was similar in larva hatched across 5 egg masses collected from 3 sites but were affected by salinity treatments. Furthermore, the (transformed) ratio of this surface area to the body length of the larva was independent of the size of C. oppositus. However, for Chironomus cloacalis, this surface area differed between larva hatched from egg masses collected from the same site. The expected trend in surface area of the anal papillae relative to the size of larva (Chironomu alternans, C. cloacalis, Dicrotendipes sp., Criptochironomus sp. and Tanypodinae) was not duplicated in the field survey. It would appear that unknown factors, other than salinity, are affecting the size of the anal papillae of chironomids in southern Victoria.  相似文献   

低分子量氨基糖及其衍生物对角膜细胞生长影响的研究     

赵国燕  刘万顺  韩宝芹  梁晔 《中国海洋大学学报(自然科学版)》2011,41(3):52-56

体外培养兔角膜上皮细胞和基质细胞,采用显微观察和MTT相结合的方法分析低分子量氨基糖(包括壳寡糖(chitosan)、羧甲基壳寡糖(Carboxymethyl chitosan oligosaccharide)、羧甲基甲壳寡糖(Carboxymethyl chitin oligosaccharide)、N-乙酰氨基葡萄糖(N-acetyl-glucosamine))对细胞生长的影响。结果在0~1000μg/mL浓度范围内4种氨基糖对角膜上皮细胞和基质细胞均没有细胞毒性,且均能促进角膜上皮细胞的生长,以壳寡糖和羧甲基甲壳寡糖效果最佳,二者与对照组相比均具有显著性差异(P<0.05)。壳寡糖、羧甲基甲壳寡糖和N-乙酰氨基葡萄糖能明显促进角膜基质细胞的生长,与对照组相比具有显著性差异(P<0.05),其中壳寡糖的促进作用最明显。提示低分子量氨基糖可适用于角膜细胞促生长的培养,为壳聚糖衍生物材料用于眼科研究提供一定的实验依据。  相似文献   

ENSO期间海洋性大陆典型地区闪电活动特征分析   总被引：2，自引：1，他引：1  

林锦冰  马明  傅云飞 《气象学报》2013,71(1):109-120

利用星载闪电探测器(光学瞬变探测器(OTD)和闪电成像传感器(LIS))的观测资料,配合其他气象资料,对厄尔尼诺期间(1998年春季)和拉尼娜期间(1999年春季)海洋性大陆典型地区(11.25°S—3.75°N,96.25°—128.75°E)的闪电活动变化特征进行研究,分析了雷暴单体数目以及雷暴单体闪电率对闪电活动变化的影响,并通过对比厄尔尼诺年春季和拉尼娜年春季的大气环流形势、相对湿度、最大对流有效位能、对流风暴高度等气象要素,讨论闪电活动变化的原因。结果表明:(1)从ENSO期间雷暴单体密度和闪电密度的空间分布变化特征来看,厄尔尼诺年春季的闪电活动及雷暴活动均比拉尼娜年春季的多,并且,从闪电数目和雷暴单体数目的纬向平均、经向平均的年际变化可以发现在厄尔尼诺年春季闪电活动、雷暴活动有东伸南移的趋势。(2)在海洋性大陆典型地区,雷暴单体数目的变化是闪电数目变化的主要因子,而雷暴单体闪电率的变化是闪电数目变化的次要因子。(3)与1999年春季相比,1998年春季的副热带高压范围大、强度大;地面相对湿度大,高空相对湿度小,上下层湿度差异大,有利于对流发展;对流风暴高度较高,冰相粒子层厚度也较深厚,对流发展旺盛;最大对流有效位能大于300 J/kg的天数的空间分布极大值区域正好与闪电密度、雷暴单体密度的大值区域对应,雷电活动与对流有效位能值密切相关。  相似文献   

Humic Acid and Iron Chelation Modified Anode Improves the Electrochemical Performance of Marine Sediment Microbial Fuel Cell     

FU Yubin  CHEN Jiaqi  CHEN Yan  HUANG Xiang  LI Yang  ZHANG Huaijing  ZAI Xuerong 《中国海洋大学学报(英文版)》2022,21(2):388-394

Marine sediment microbial fuel cell(MSMFCs)can be utilized as a long lasting power source to drive small instruments to work for long time on ocean floor and its higher power has a significant meaning for practical application.Anode modification can greatly improve the performance of MSMFCs.Herein,humic acid(HA)and humic acid-iron ion complex(HA-Fe)were used to modify the anode for constructing a better MSMFCs.The results indicated that HA-Fe modified anode,better than HA modification,significantly improved the MSMFCs cell power output.The maximum power density of HA-Fe modified MSMFCs is 165.3 mW m?2,which are 6.5-folds of blank MSMFCs.The number of microorganisms on anode,redox activity,and relative kinetic activity were 1.8-,6.1-,and 13.1-folds of blank MSMFCs,respectively.The MSMFCs improvement would be attributed to the electron transfer media of HA and the valence conversion of Fe ions.A synergistic interaction between the naturally occurring HA and Fe ions on the anodic surface in marine sediments would make the modified anodes have‘renewable’characteristics,which is beneficial for the MSMFCs to maintain its long-term higher power.  相似文献