Optimization of Airborne Real-time Cueing Hyperspectral Enhanced Reconnaissance (ARCHER) imagery,in situ data with chemometrics to evaluate nutrients in the Shenandoah River,Virginia     

Mbongowo J. Mbuh 《国际地球制图》2013,28(12):1326-1349

Abstract

Phosphorus and nitrogen have a strong influence on water resource and remote sensing technology has demonstrated that water quality monitoring over a greater range of temporal and spatial scales can be used to overcome these constraints. This research was designed to demonstrate the feasibility of combining remotely-sensed water quality observation and chemometric techniques to estimate water quality in the Shenandoah River. We used Airborne Real-time Cueing Hyperspectral Enhanced Reconnaissance (ARCHER) imagery, combined with a partial least squares analysis to characterize the spatial distribution of nutrients in the Sheanadoah river. ARCHER retrievals for phosphorous with cross-validation show high sensitivity in estimating water quality in the Shenandoah River with the Bentonville in the South Fork, with an R2 of 0.93 sensitivity. Using the significance level of 0.05, data from the summer of 2014 showed that the p-value was 0.00 for both nitrogen and phosphorous. Results show retrieval method is transferable.  相似文献   

拟穴青蟹(Scylla paramamosain)PP2A调节亚基B的基因克隆与表达分析     

刘学良  金朱兴  黄辉洋  叶海辉  李少菁 《海洋与湖沼》2012,43(5):932-937

采用RT-PCR、RACE等方法,获得了拟穴青蟹PP2A调节亚基B(PP2A-B)的cDNA全序列,全长2040bp,开放阅读框(ORF)为1332bp,可编码443个氨基酸残基。同源分析显示,该基因编码的蛋白与其它一些物种具有很高相似性,推测PP2A-B基因具有很高的保守性。经荧光定量PCR检测,PP2A-B基因在拟穴青蟹多个组织中有表达,且在脑、卵巢、鳃中表达量较高。在拟穴青蟹卵巢发育过程中,PP2A-B基因在卵巢未发育期(I期)表达量最高,此后各期逐渐下降,推测PP2A-B在卵巢中可能以PP2A全酶的形式存在,抑制卵巢发育。  相似文献   

浙江枝吻纽虫(Dendrorhynchus zhejiangensis) 热休克蛋白70基因克隆与表达特征研究     

路雅丽  李成华  苏秀榕  李太武  朱琳  张鹏  李晔 《海洋与湖沼》2012,43(6):1156-1162

采用同源克隆和cDNA末端快速扩增技术(RACE)克隆了浙江枝吻纽虫热休克蛋白70(DzHSP70)基因全长cDNA序列,并利用荧光定量PCR方法,分析了该基因在重金属胁迫下的表达特征。结果表明,DzHSP70cDNA全长2827bp,包括294bp的5’UTR、628bp的3’UTR和编码634个氨基酸残基的1905bp的开放阅读框。HSP70家族的3个签名序列以及细胞质特异性调控基序EEVD等特征元件在DzHSP70中高度保守。重金属离子Fe3+、Pb2+和Cd2+均可上调该基因的表达,其中Pb2+的毒理效应最强。研究结果表明DzHSP70可能参与了机体对于重金属的解毒过程。  相似文献   

饲料中添加益生菌对凡纳滨对虾(Litopenaeus vannamei)非特异免疫基因表达量和抗病力的影响   总被引：2，自引：0，他引：2  

孙艳  刘飞  宋晓玲  麦康森  李玉宏  黄倢 《海洋与湖沼》2012,43(4):845-851

采用实时荧光定量PCR方法,进行肠道复合益生菌对凡纳滨对虾相关免疫基因表达量的研究。通过感染实验,分析饲料中添加益生菌对凡纳滨对虾病毒感染能力的影响。结果表明,Real-time PCR揭示在投喂期间饲料中添加芽孢杆菌/溶藻弧菌组的对虾血淋巴细胞中的proPO、SOD和LZM的相对表达量均显著高于对照组(P<0.05);感染WSSV后,对芽孢杆菌/溶藻弧菌组凡纳滨对虾血淋巴7个采样时间点的proPO、SOD、LZM的表达进行Real-time PCR分析,检测表明WSSV刺激24h后,对虾血淋巴中的proPO、SOD和LZM的表达呈现显著性上调,且分别在48h、96h、96h达到最大值。累积死亡率结果证实饲料中添加益生菌均可提高其对虾抗WSSV感染的能力,尤其以坚强芽孢杆菌活菌与溶藻弧菌活菌配合使用效果更佳。  相似文献   

基于CORS网络的单频GPS实时精密单点定位新方法   总被引：3，自引：0，他引：3       下载免费PDF全文

姜卫平  邹璇  唐卫明 《地球物理学报》2012,55(5):1549-1556

利用单频接收机实现大范围、高精度快速实时定位是卫星大地测量研究的热点和难点问题之一.本文提出了一套基于连续运行参考站系统(CORS)的误差实时建模数据处理方法,设计并实现了一种新的GPS单频接收机实时精密单点定位服务方法.然后,利用山西省CORS网内一组实测动态数据,以及从湖北省CORS网和武汉市CORS网选取的一组实测静态数据进行测试,验证分析了所提出方法用于实时定位服务的可行性.结果表明:对于站间平均距离小于71 km的参考网,网内单频用户按照实时处理模式,在经过数秒钟的初始化后即可获得厘米级的动态精密单点定位结果.这为GPS单频接收机用户实现快速实时精密单点定位提供了一种可行的新方法.  相似文献   

The Southern Ontario All-sky Meteor Camera Network     

R. J. Weryk  P. G. Brown  A. Domokos  W. N. Edwards  Z. Krzeminski  S. H. Nudds  D. L. Welch 《Earth, Moon, and Planets》2008,102(1-4):241-246

We have developed an automated network of all-sky CCD video systems to detect medium–large meteoroids ablating over Southern Ontario, Canada. The system currently consists of five stations with the largest baseline being 180 km. Each site runs a video rate recorder with sufficient resolution to determine meteoroid trajectories with a typical precision of about 300 m but no worse than 1 km. The sensitivity of the camera is close to a stellar visual magnitude of +1 which allows for astrometric calibrations using field stars. Photometric procedures have also been developed and tested. The system has a limiting magnitude for meteors of about −2 with the current detection algorithm.  相似文献   

大竹蛏(Solen grandis)翻译控制肿瘤蛋白(TCTP)基因的分子克隆和表达特征分析     

韦秀梅  杨顶珑  杨建敏  杨嘉龙  徐洁  刘相全  张艳敏 《海洋与湖沼》2013,44(3):584-589

本研究克隆得到了一个大竹蛏(Solen grandis)翻译控制肿瘤蛋白(TCTP)基因(SgTCTP)的cDNA全长,其序列全长为1055bp,5′和3′端的非编码区(UTR)分别为54和461bp,开放阅读框(ORF)540bp,编码179个氨基酸,理论等电点为4.50,预测分子量大小19.96kDa。通过荧光定量PCR法检测了SgTCTP在健康大竹蛏各组织中和病原相关分子模式(PAMPs)刺激后的表达规律,结果表明:SgTCTP在检测组织外套膜、鳃、性腺、血细胞、肌肉和肝胰腺中都有表达,其中在肝胰腺中的表达量最高。脂多糖(LPS)、肽聚糖(PGN)和葡聚糖(β-1,3-glucan)刺激都能诱导SgTCTP的表达量上调,SgTCTP的表达量分别在LPS和PGN刺激后6和3h达到最高,为空白对照的3.64和3.36倍;β-1,3-glucan刺激后SgTCTP的表达量上升幅度最大,在12h达到最高,为空白对照的11.76倍。SgTCTP可能作为急性时相蛋白参与大竹蛏的免疫应答。  相似文献   

Experimental test of asymmetrical cable-stayed bridges using MR-damper for vibration control     

《Soil Dynamics and Earthquake Engineering》2014

In this paper, a semi-active control by MR-damper is researched; its purpose is to effectively control vibration of asymmetrical cable-stayed bridges when earthquake is loaded on the type of bridge. For an experimental study, a model of 10.2 m high and 28 m long asymmetrical cable-stayed bridge structure was built being similar to a real one in size and function. A MR damper was also designed in proper size suitable for the control of the model. The experiment was performed in the way in which three piers were fixed on three shaking tables with 30% of El-centro earthquake wave, and a control device was placed on the lower part of its upper deck for horizontal control. As for control algorithms, Lyapunov and Clipped-optimal control algorithms were applied. The effectiveness of the semi-active control with MR damper for the asymmetrical cable-stayed bridge was measured under five control conditions: Un-control, Passive-off, Passive-on, Lyapunov Control, Clipped-optimal Control. The experiment showed that the semi-active control applying Lyapunov and Clipped-optimal algorithms effectively increased controllability almost in double, and decreased displacement 75% compared with the condition of passive-off. Therefore the semi-active method suggested in this paper is proven effective in controlling asymmetrical cable-stayed bridges.  相似文献   

X射线极光强度与亚暴指数AE的相关关系          下载免费PDF全文

赵亮  涂传诒 《地球物理学报》2005,48(4):739-743

研究了Polar卫星的极区电离层X射线成像仪（PIXIE）得到的极光X射线成像强度AI（Auroral Intensity）与磁层亚暴指数的相关关系.本文发现,在所选取的1997年至2001年的部分数据中,从完整的X射线图像得到的极光X射线总强度和AE指数有很好的线性相关关系,在全部83组数据中有566％的数据的线性相关系数都在060以上（相关系数最大为097）.所以本文认为极光X射线总强度可以作为新的磁层亚暴卫星实时监测指数.  相似文献   

大黄鱼EBI3基因的克隆与表达分析          下载免费PDF全文

霍洁颖  刘小玲  林蠡  陈新华 《应用海洋学学报》2017,36(3):403-410

通过克隆大黄鱼(Larimichthys crocea)的EBI3(Epstein-Barr virus-induced gene 3)基因并对其进行了序列分析.大黄鱼EBI3(Lyc EBI3)基因开放阅读框长747bp,编码248个氨基酸,存在1个信号肽序列和2个FN3结构域(37~130,145~230).多序列比对发现Lyc EBI3分子与其他已知EBI3氨基酸水平一致性为27.68%~57.53%.Real-time PCR结果表明,Lyc EBI3基因在脾脏和血液中表达量最高,且溶藻弧菌(Vibrio alginolyticus)刺激后,大黄鱼头肾和脾脏中Lyc EBI3基因的转录水平会显著升高,说明Lyc EBI3基因可能参与了抑制由细菌引起的炎症反应.  相似文献