短蛸(Octopus ocellatus)酪氨酸蛋白激酶Btk基因的克隆及其mRNA在细菌刺激后的表达规律_*     

张冉冉  韦秀梅  杨建敏  王卫军  刘相全  冯艳微 《海洋与湖沼》2015,46(1)

本研究从前期构建的短蛸cDNA文库中克隆获得了一个短蛸(Octopus ocellatus)酪氨酸蛋白激酶(OoBtk)基因的cDNA全长,其cDNA全长1191 bp,包括5’非编码区(UTR)259 bp,3’UTR 227 bp,开放阅读框(ORF)705 bp,编码234个氨基酸,预测理论等电点为8.50,分子量为27.7 kD。运用实时荧光定量PCR法分析了OoBtk在健康短蛸不同组织及鳗弧菌、藤黄微球菌刺激后短蛸血细胞中的表达规律,结果表明：OoBtk在健康短蛸肌肉、外套膜、鳃、鳃心、系统心脏、肝胰脏、肾囊、胃、性腺和血细胞等各检测组织中均有表达,其中在肝胰脏的表达量最高;短蛸经鳗弧菌和藤黄微球菌刺激后,OoBtk在血细胞中的表达量呈现出了明显的被诱导表达趋势,在鳗弧菌和藤黄微球菌刺激后6 h后表达即增强,并分别在鳗弧菌刺激后48 h和藤黄微球菌刺激后12 h达到最高。OoBtk参与短蛸对于鳗弧菌和藤黄微球菌刺激的应答过程。  相似文献   

Incorporation of neural network-like processors for GPS navigation     

Dah-Jing Jwo 《GPS Solutions》2004,8(3):160-169

The solution for the receivers position and clock bias using four or more GPS pseudorange measurements involves nonlinear quadratic equations. One of the popular techniques for linearizing the equations and solving them is the least squares (LS) scheme based on an iterative gradient approach. For real-time applications when the solution is to be obtained within a time of the order of 100 ns, a computer often cannot comply with the desired computation time, or high-end computers are too expensive. In this paper various ordinary differential equation formulation schemes, and corresponding circuits of neuron-like analog processors, will be described and several tested in order to ascertain their suitability for GPS navigation processing purposes. The circuits of simple neuron-like analog processors are employed essentially for on-line inversion of matrices, which is usually required for determining LS solutions, as well as dilution of precision (DOP) calculation in standard GPS receivers. Data from single epoch and kinematic positioning experiments will be simulated to validate the effectiveness of the proposed scheme. The properties and performance of the proposed scheme will be assessed and compared to those of the conventional method of matrix inversion.  相似文献   

Regulation of Tilapia metallothionein gene expression by heavy metal ions     

Andrew P. L. Cheung  Timothy H. -J. Lam  King Ming Chan   《Marine environmental research》2004,58(2-5):389

Tilapia is a common fish species inhabiting inland waters and estuarine regions in Hong Kong and Southeast Asia, and useful for bio-monitoring of metal pollution. Metallothionein (MT) gene expression in fish tissues has been useful to sub-lethal risk assessment as biomarker of exposure to metal ions in fishes inhabiting metal contaminated area. To investigate metal inductions of Tilapia MT gene expression in vivo, Tilapias were injected with different concentrations of heavy metals and tissues were then removed for quantitative PCR assay using mimic PCR methods. All of the metal ions tested (Cu²⁺, Cd²⁺, Hg²⁺, Ni²⁺, Pb²⁺ and Zn²⁺) were able to induce hepatic MT mRNA levels. Renal MT mRNA levels of Cd²⁺ and Zn²⁺ treated fish was not induced with significant fold induction, however MT mRNA levels in gills were sensitive to the administrations of these metal ions. These data indicated that Tilapia MT mRNA levels in gills and liver are sensitive biomarker of exposure to various metal ions.  相似文献   

Precision real-time navigation of LEO satellites using global positioning system measurements   总被引：4，自引：3，他引：1  

Oliver Montenbruck  Pere Ramos-Bosch 《GPS Solutions》2008,12(3):187-198

Continued advancements in remote sensing technology along with a trend towards highly autonomous spacecraft provide a strong motivation for accurate real-time navigation of satellites in low Earth orbit (LEO). Global Navigation Satellite System (GNSS) sensors nowadays enable a continuous tracking and provide low-noise radiometric measurements onboard a user spacecraft. Following the deactivation of Selective Availability a representative real-time positioning accuracy of 10 m is presently achieved by spaceborne global positioning system (GPS) receivers on LEO satellites. This accuracy can notably be improved by use of dynamic orbit determination techniques. Besides a filtering of measurement noise and other short-term errors, these techniques enable the processing of ambiguous measurements such as carrier phase or code-carrier combinations. In this paper a reference algorithm for real-time onboard orbit determination is described and tested with GPS measurements from various ongoing space missions covering an altitude range of 400–800 km. A trade-off between modeling effort and achievable accuracy is performed, which takes into account the limitations of available onboard processors and the restricted upload capabilities. Furthermore, the benefits of different measurements types and the available real-time ephemeris products are assessed. Using GPS broadcast ephemerides a real-time position accuracy of about 0.5 m (3D rms) is feasible with dual-frequency carrier phase measurements. Slightly inferior results (0.6–1 m) are achieved with single-frequency code-carrier combinations or dual-frequency code. For further performance improvements the use of more accurate real-time GPS ephemeris products is mandatory. By way of example, it is shown that the TDRSS Augmentation Service for Satellites (TASS) offers the potential for 0.1–0.2 m real-time navigation accuracies onboard LEO satellites.  相似文献   

基于声光信号的单站式闪电三维通道定位系统     

张传亮  孙学金  卫克晶  张日伟  颜万祥  李浩然 《气象科学》2016,36(4):502-509

目前基于雷声的三维定位系统多停留在实验阶段,从业务化运行的角度考虑,改进了单站式闪电三维定位系统的麦克风阵列,设计了定位算法,利用该系统采集了2014年夏季南京地区的部分闪电数据,并从计算耗时的角度对三维通道实时定位的可行性进行了研究。结果表明,基于声光信号的单站式闪电三维通道定位系统可重构闪电的三维通道;对连续的雷声信号进行分帧,帧长取0.03 s时,声源定位点可描绘出清晰的闪电主通道;采用分组保存和处理闪电声光信号的方法,可实现实时定位,当一组数据的保存时间为50 s时,计算耗时为46.507 s。  相似文献   

基于IGU星历的实时卫星钟差加密方法研究     

刘朋姣  陈义 《全球定位系统》2010,35(6):35-39

主要介绍了利用IGS提供的超快速观测星历和预报星历进行5 min和30 s实时卫星钟差获取的方法,并对利用这两种星历文件获取的卫星钟差与IGS提供的最终星历做比较,最后得出结论：基于IGS的超快速观测星历采用线性模型预报实时卫星钟差的精度高于基于IGS超快速预报星历采用三次样条函数直接内插获得的实时卫星钟差的精度,可以达到3 ns,能够满足一般实时单点定位的精度要求。  相似文献   

市级灾害性天气应急服务系统   总被引：4，自引：2，他引：2       下载免费PDF全文

鄢志宇  杨桂娟  鲁建东  郝伟 《气象与环境学报》2009,25(1):54-57

灾害性天气应急服务系统是市级气象台根据实际工作需要,研制开发的灾害性天气监测、预报预警业务系统。系统实现了对灾害性天气的实时监测,预警信息编辑、制作和发布,灾情快速评估及极端灾害性天气预报应急服务等功能。介绍了系统的功能特点和业务应用情况。为市级台站做好灾害性天气监测、预报和预警应急服务等工作提供了可借鉴的思路。  相似文献   

高频GNSS实时地震学与地震预警研究现状   总被引：1，自引：0，他引：1       下载免费PDF全文

单新建  尹昊  刘晓东  王振杰  屈春燕  张国宏  张迎峰  李彦川  汪驰升  姜宇 《地球物理学报》2019,62(8):3043-3052

为实现从注重灾后救助向注重灾前预防转变,如何提高地震灾害监测预警和风险防范能力成为我们关注的重点.本文给出了国际上GNSS位移记录、强震动加速度记录、测震速度记录在地震预警中的应用现状,并总结了各自的特点,归纳出围绕高频GNSS地震学在震级与破裂过程实时反演中的几个需要进一步研究的关键问题：（1）引入北斗系统,基于高频GNSS（GPS/BDS）双系统的实时位移解算方法来提高实时单站位移解算精度,使实时解算精度达到厘米级;（2）开展强震仪加速度记录基线偏移校正研究,弥补地震近场GNSS站密度不足问题;（3）强震仪加速度记录与GNSS位移记录特点不同,开展强震仪加速度数据与GNSS位移数据实时融合处理研究,快速获得包含丰富地震形变和速率的波形数据;（4）测震学方法可快速估算震级,但是在强震发生时会出现震级饱和现象,造成震级估算偏低.需要开展基于GNSS位移时间序列的多种方法相结合的实时震级估算方法研究,通过与地震学方法比较和结合,来得到精度高、计算快的震级估值算法;（5）基于高频GNSS、断层初始模型快速选取、断层尺度、参数自适应调整是快速判断断层破裂方向的基础,在断层破裂过程自适应准实时反演算法方面需要进一步加强.通过国内外研究现状调研、分析,表明基于高频GNSS地震学的震级快速确定、震源破裂过程准实时反演算法的发展将对我国地震预警系统从"二网融合"到"三网融合"提供坚实的技术支撑.  相似文献   

大斜度井/水平井随钻方位电磁波测井资料实时反演方法   总被引：3，自引：0，他引：3       下载免费PDF全文

王磊  范宜仁  操应长  邓少贵 《地球物理学报》2020,63(4):1715-1724

水平井钻进过程中,地层边界的准确预测对地质导向决策至关重要.但水平井测井环境复杂,随钻方位电磁波测井响应不直观,储层性质、地层界面等信息的精确、定量评价困难.本文提出了一种适用于大斜度井/水平井随钻方位电磁波测井资料的电阻率及地层界面信息的实时提取方法.该方法主要基于以下几个技术:(1)引入滑动开窗策略将高维随钻方位电磁波测井资料的反演问题转化为多个连续窗口的一维反演问题;(2)对反演参数进行多初值初始化,结合正则化Levenberg-Marquardt算法保证解的全局最优性;(3)充分利用随钻方位电磁波测井多种探测深度信息,采用分级反演策略提高反演速度.数值和实测资料反演结果表明:降维反演方法可实现对地层界面的实时、准确提取,且适用于多数复杂地层结构和任意井眼轨迹;利用浅探测地质信号可实现随钻方位电磁波测井资料的分级反演,最终反演速度可提升10倍以上.  相似文献   

An ANN algorithm for automatic, real-time tsunami detection in deep-sea level measurements     

Gian Mario Beltrami   《Ocean Engineering》2008,35(5-6):572-587

The present paper looks at algorithms to be implemented in the software of bottom pressure recorders (BPRs) for the automatic, real-time detection of a tsunami within recorded signals. The structure of an algorithm based on the use of an artificial neural network (ANN) is presented and compared to the one developed under the Deep-ocean Assessment and Reporting of Tsunamis (DART) program run by the U.S. National Oceanic and Atmospheric Administration (NOAA). The performance and efficiency of the two algorithms are compared using both synthetic and actually measured time series. Results show that an improvement in detection performance can be obtained by using the ANN algorithm.  相似文献