iTRAQ-based proteomic profiling of the marine medaka (Oryzias melastigma) gonad exposed to BDE-47     

C.C. Fong  Y.F. Shi  W.K. Yu  F. Wei  J.P. van de Merwe  Alice K.Y. Chan  R. Ye  Doris W.T. Au  Rudolf S.S. Wu  M.S. Yang 《Marine pollution bulletin》2014

A recent study demonstrated that 2,2′,4,4′-tetrabromodiphenyl ether (BDE-47) may have an adverse effect on the reproduction in marine medaka (Oryzias melastigma), but the molecular mechanisms remain largely unknown. In this study, we investigated the protein expression profiles of male and female gonads of O. melastigma exposed to dietary BDE-47 at two dosages (0.65 and 1.30 μg/g/day, respectively) for 21 days. Extracted proteins were labeled with iTRAQ and analyzed on a MALDI TOF/TOF analyzer, as results, 133 and 144 unique proteins were identified in testis and ovary, respective, and they exerted dose- and sex-dependent expression patterns. In testis, among the 42 differentially expressed proteins; down-regulation of histone variants and parvalbumins implicated BDE-47 may disrupt the spermatogenesis and induce sterility in fishes. In ovary, 38 proteins were differentially expressed; the elevation of vitellogenins and apolipoprotein A–I expression indicated BDE-47 acts as an estrogen-mimicking compound and led to reproductive impairment in O. melastigma.  相似文献   

Solution of dynamic Green׳s function for unsaturated soil under internal excitation     

《Soil Dynamics and Earthquake Engineering》2014

The closed form three-dimensional Green׳s function of a semi-infinite unsaturated poroelastic medium subjected to an arbitrary internal harmonic loading is derived, with consideration of capillary pressure and dynamic shear modulus varying with saturation. By applying the Fourier expansion techniques and Hankel integral transforms to the circumferential and radial coordinates, respectively, the general solution for the governing partial differential equations is obtained in the transformed domain. A corresponding boundary value problem is formulated. The integral solutions for the induced displacements, pore pressure and net stress are then determined considering the continuity conditions. The formulas are compared with the degenerated solution of saturated soils and confirmed. Numerical results reveal that the response of the unsaturated half-space depends significantly on the saturation by altering dynamic shear modulus to account for the effects of matric suction on soil stiffness. Slight differences between the results occur if only the saturation is taken into account. Moreover, a large source-depth results in a pronounced contribution to the reduction of surface displacement amplitudes. The analytical solutions concluded in the study offer a broader application to dynamic response associated with axi-symmetric and asymmetric conditions.  相似文献   

珠三角县域城市潜力的时空关联结构演变     

梅志雄徐颂军  欧阳军 《地理学报》2014,69(4):497-509

基于1990、1994、2000、2005 和2009年公路网数据和经因子分析得到的县域城市综合实力指数,采用扩展的潜力模型测度县域潜力指数,运用时空自相关方法探讨珠三角县域潜力的时空关联格局及其演变,并与空间关联格局对比及与交叉相关函数比较检验其准确性。结果表明：① 珠三角县域潜力总体上具正时空关联性,但关联程度不高,1994-2005年缓慢加强而2005-2009年趋于减弱。大多县域潜力的局部时空关联特征相对稳定,且以正相关为主,但部分县市时空关联类型发生了明显转化。② 县域潜力的局部时空关联类型分布差异及其变化明显,时空HH区分布于中部地带和东部的深圳—东莞地带,2000年后中部的时空HH区范围收缩至广—佛核心都市区;西部时空LL区连片分布格局相对稳定,中南部2005年开始出现新的时空LL区,东部时空LL区范围1994-2000年扩大而后逐步收缩,2009年零星分布于东部边缘;时空异质区变化较剧烈。③ 三大地带县域潜力的局部时空关联特征反差鲜明,东部与中部差异趋于缩小,而西部与中、东部的差异进一步扩大。④ 时空自相关方法能有效挖掘县域潜力的时空关联模式,比仅考虑空间效应的ESDA方法更能准确客观揭示县域潜力间的时空交互关系。  相似文献   

利用背景噪声反演鄂尔多斯块体及其南缘地区地壳速度结构   总被引：1，自引：0，他引：1  

陈强森  鲍学伟  徐树斌  王良书  徐鸣洁 《高校地质学报》2013,19(3):504-512

文中利用分布在鄂尔多斯块体及其南部周缘地区的53 个宽频带地震固定台站的连续波形记录,采用双台互相关计算 方法由背景噪声提取瑞利波格林函数,经时频分析获得相速度和群速度频散曲线,并分别计算了汾渭地堑、秦岭北缘、鄂 尔多斯块体内部和六盘山地区4 个不同构造区的平均频散曲线,进而反演了各构造区的地壳上地幔一维横波速度结构。结 果显示：地壳厚度在汾渭地堑为34 km,在秦岭北缘地区和鄂尔多斯块体均为40 km,在六盘山地区最厚,达49~50 km;相 应的上地幔顶部横波速度分别为4.20,4.2,4.30 和4.15 km/s;地壳内结构浅部特征差异最大,在地壳中部六盘山地区的速 度较低,下部地壳不同地区的波速较一致。  相似文献   

SH波上方垂直入射时界面附近椭圆夹杂与裂纹的动态响应     

杨在林  许华南  黑宝平 《岩土力学》2013,34(8):2378-2384

采用Green函数、保角映射等方法分析SH波上方垂直入射时弹性半空间中椭圆形弹性夹杂与任意方位裂纹的相互作用。首先,求解含椭圆形弹性夹杂的弹性半空间内任意一点承受时间谐和出平面线源荷载作用时的位移基本解,即Green函数,再结合裂纹切割法构造裂纹,得到SH波上方垂直入射时椭圆夹杂和裂纹同时存在条件下的位移场与应力场。作为算例,给出大量的数值结果,探讨不同参数情况下弹性夹杂周边动应力集中系数（DSCF）、地表位移及裂纹尖端动应力强度因子（DSIF）的分布规律,结果表明不同参数的变化会对三者的分布带来一定的影响。  相似文献   

Genetic diversity and population structure of Eleutheronema rhadinum in the East and South China Seas revealed in mitochondrial COI sequences   总被引：1，自引：0，他引：1  

孙鑫序  徐冬冬  楼宝  张涛  辛俭  郭垚示  马世磊 《中国海洋湖沼学报》2013,31(6):1276-1283

Eleutheronema rhadinum is a potential commercial fisheries species and is subject to intense exploitation in China. Knowledge on the population structure of E. rhadinum in Chinese coastal waters, which is important for sustainable exploitation and proper resource management, is lacking. In the present study, the genetic diversity and population structure of E. rhadinum were evaluated using a 564-base pair fragment of the mitochondrial cytochrome c oxidase subunit I （COl） gene. A total of 76 specimens were collected from three localities around the East （Qidong and Zhoushan） and South China Seas （Zhuhai）. Among these individuals, nine polymorphic sites were detected and 1 l distinct haplotypes were defined. High levels ofhaplotype diversity （h=0.759i0.035） and low levels of nucleotide diversity （re=0.001 98i0.003 26） were observed in these populations, Hierarchical analysis of molecular variance （AMOVA） indicated that 96.72% of the genetic variation occurred within the populations, whereas 3.28% occurred among populations. No significant genealogical branches or clusters were recognized on the neighbor-joining tree. Intra-group variation among populations was significant （~0~t=0.032 85, P〈0.01）. These results suggest that E. rhadinum populations in the East and South China Seas have developed divergent genetic structures. Tests of neutral evolution and mismatch distribution suggest that E. rhadinum may have experienced a population expansion. The present study provides basic information for the conservation and sustainable exploitation of this species.  相似文献   

地下核爆炸地震波二次源特征   总被引：4，自引：1，他引：3       下载免费PDF全文

何永锋  赵克常  张献兵  马裕然  王洋 《地球物理学报》2012,55(5):1742-1748

层裂是地下核爆炸震源物理过程中最重要的非球对称源过程.对其机制的研究,多基于面波资料,本文基于远场体波资料,利用经验格林函数方法,消除路径及接收场地效应,利用地下核爆炸远场地震波P波数据对震源时间函数进行了反演,结果显示明显的双脉冲特征,该二次源特征不随方位角及震中距变化,说明它源自震源本身,其双脉冲特征与层裂机制相符.  相似文献   

Comparing long term sediment records to current biological quality element data - Implications for bioassessment and management of a eutrophic lake     

Tommi Kauppila  Antti Kanninen  Matias ViitasaloJohanna Räsänen  Kristian MeissnerJukka Mattila 《Limnologica》2012,42(1):19-30

Defining reference conditions for lakes situated in areas of human settlement and agriculture is rarely straightforward, and is especially difficult within easily eroding and nutrient rich watersheds. We used diatoms, cyanobaterial akinetes, remains of green algae and chironomid head capsules from sediment samples of Lake Kirmanjärvi, Finland, to assess its deviation from the initial ecological status. These site-specific records of change were compared to current type-specific ecological status assessment. All paleolimnological data indicated deviation from natural conditions and mirrored the current, monitoring-based assessment of “moderate” ecological lake status. However, the sediment data showed that the lake should be re-typified as a naturally eutrophic lake. Sediment records as well as current monitoring data indicated temporary improvement in water quality in response to extensive fish manipulation. Our results suggest that paleolimnological records can be used to derive site-specific reference conditions and that extensive restoration efforts can result in gradual, observable improvements of water quality and ecological status.  相似文献   

利用噪声记录估计福建地区中上地壳体波速度结构     

李军  金星  鲍挺  林树  韦永祥  张红才 《中国地震研究》2012,26(3):310-320

In this paper,the dispersion curves of the Rayleigh wave and Love wave were extracted from the seismic noise records of 25 broadband stations of the Fujian Seismic Network, and inverted for the lithosphere velocity structure. Furthermore,the velocity model was verified by the seismic explosion observations. Our results indicate that the resolution of the lithosphere velocity structure obtained by this method is good in the shallow part,but in the deep part,inversion accuracy for the wave velocity structure is low,which is caused mainly by the small inter-station distance chosen in the paper. Thus the wave dispersion curves have high accuracy in the short-period part,but the warp of the wave dispersion curve in long-period part is large. Considering the results from both the noise inversion and the traditional inversion,we finally present a new velocity model,and the theoretical travel time calculated with the new model matches the explosion travel time very well.  相似文献   

Sensitive Ion‐Flotation Separation of Ag(I) Traces Using 2‐(2‐Methoxyphenyl)benzimidazole before Flame Atomic Absorption Spectrometric Determination in Water     

Hossein Tavallali  Shahram Lalehparvar  Abdo‐Reza Nekoei  Maryam Ghane Jahromi  Khodabakhsh Niknam 《洁净——土壤、空气、水》2012,40(6):640-647

A sensitive, reliable, and environmentally friendly method for simple separation and preconcentration of Ag(I) traces in aqueous samples is presented prior to their flame atomic absorption spectrometric determinations. At pH 7.0, Ag(I) was separated with 2‐(2‐methoxyphenyl)benzimidazole (MPBI) as a new complexing agent and floated after adding sodium dodecyl sulfate (SDS) as a foaming reagent. The floated layer was then dissolved in proper amount of concentrated nitric acid in methanol and introduced to the flame atomic absorption spectrometer (FAAS). The effects of pH, concentration of MPBI, type and amount of surfactant as the floating agent, type and amount of eluting agent, and influence of foreign ions on the recovery of the analyte ion were investigated. Also, using a nonlinear curve fitting method, the formation constant of 1.62 × 10⁶ was obtained for Ag(I)–MPBI complex. The analytical curve was linear in the range of 1.8 × 10^?7–1.7 × 10^?6 mol/L for determination of Ag(I). The relative standard deviation (RSD; N = 10) corresponding to 0.7 × 10^?6 mol/L of Ag(I), the limit of detection (10 blanks), and the enrichment factor were obtained as 1.7%, 2.9 × 10^?8 mol/L, and 43.0, respectively. The proposed procedure was then applied successfully for determination of silver ions in different water samples.  相似文献