全文获取类型
收费全文 | 338篇 |
免费 | 32篇 |
国内免费 | 122篇 |
专业分类
测绘学 | 8篇 |
大气科学 | 8篇 |
地球物理 | 85篇 |
地质学 | 68篇 |
海洋学 | 260篇 |
综合类 | 44篇 |
自然地理 | 19篇 |
出版年
2024年 | 8篇 |
2023年 | 17篇 |
2022年 | 19篇 |
2021年 | 13篇 |
2020年 | 15篇 |
2019年 | 19篇 |
2018年 | 15篇 |
2017年 | 13篇 |
2016年 | 11篇 |
2015年 | 7篇 |
2014年 | 21篇 |
2013年 | 22篇 |
2012年 | 22篇 |
2011年 | 27篇 |
2010年 | 17篇 |
2009年 | 25篇 |
2008年 | 27篇 |
2007年 | 27篇 |
2006年 | 22篇 |
2005年 | 14篇 |
2004年 | 17篇 |
2003年 | 10篇 |
2002年 | 14篇 |
2001年 | 12篇 |
2000年 | 12篇 |
1999年 | 11篇 |
1998年 | 7篇 |
1997年 | 7篇 |
1996年 | 7篇 |
1995年 | 6篇 |
1994年 | 4篇 |
1993年 | 7篇 |
1992年 | 8篇 |
1991年 | 1篇 |
1990年 | 1篇 |
1989年 | 1篇 |
1988年 | 1篇 |
1986年 | 1篇 |
1985年 | 2篇 |
1982年 | 2篇 |
排序方式: 共有492条查询结果,搜索用时 15 毫秒
11.
利用牙鲆鳃细胞系分离和培养淋巴囊肿病毒 总被引:1,自引:4,他引:1
本文利用牙鲆鳃细胞系进行了养殖牙鲆淋巴囊肿病毒的分离及培养 ,并通过电镜对培养细胞中淋巴囊肿病毒的形态及感染循环进行了初步研究。将病鱼的淋巴囊肿组织无菌滤液接种牙鲆细胞系 ,细胞出现了明显的细胞病变 ( Cytopathic effect,CPE)。电镜观察在培养细胞的胞质中有病毒的包涵体 ,胞质中散在 6角形、5角形或圆形的病毒粒子 ,大小为 10 0~ 140 nm之间。在感染细胞的线粒体中也存在大量的病毒颗粒。 相似文献
12.
病毒是非常微小的简单生物, 不能独立生存, 必须借助宿主细胞完成自身的繁衍。病毒侵染进入细胞后, 通常借助于微管通过黏稠的细胞质运动到特定的复制位点。然而, 有关病毒依赖微管运动行为的精细动态研究还比较少。石斑鱼虹彩病毒(Singapore grouper iridovirus, SGIV)为虹彩病毒科蛙病毒属的一个新种, 是海水养殖鱼类的重要病毒性病原, 对海水养殖业造成重大经济损失。利用单粒子示踪技术实时追踪了SGIV病毒粒子沿微管运动的行为, 观察到SGIV在细胞边缘至微管中心之间的双向运动, 最高瞬时速度约0.2μm·s-1, 均表现为主动运输。病毒粒子运动至微管交叉位置会减速迂回, 而后或受限于此, 平均运动速率约0.008μm·s-1, 或通过交叉处继续快速运动, 最高瞬时速度为0.2μm·s-1。同时, SGIV感染会影响微管的形态结构, 随着SGIV感染, 微管逐渐围绕细胞核和病毒加工厂形成环状结构。研究结果初步揭示了SGIV病毒和细胞微管之间相互作用的复杂过程, 丰富了我们对虹彩病毒胞内生命活动的认识, 有助于深入地理解海水鱼类虹彩病毒感染致病机理。 相似文献
13.
Andrea Lami Frank Niessen Piero Guilizzoni Julieta Masaferro Claudio A. Belis 《Journal of Paleolimnology》1994,10(3):181-197
We use palaeolimnological techniques to reconstruct the eutrophication history of a volcanic lake (Lake Albano, central Italy) over the past three centuries. The presence of annual varves down to the bottom of the core (c. 1700 A.D.) indicated the lack of bioturbation and likely long-term meromixis. Sedimentation rates were estimated by varve counts (calcite/diatom couplets), indicating a mean rate of 0.15 cm yr–1. The reconstruction of eutrophication was traced using past populations of algal and photosynthetic bacteria (through their fossil pigment), and geochemistry, as well as fossil remains of chironomids. Phaeophorbidea and the red carotenoid astaxanthin were used to detect past zooplankton development.The first sign of trophic change related to human activities is datedc. 1870 A.D. From that period onward a sharp increase of authigenic CaCO3, nitrogen, N:P ratio, and dinoxanthin, a characteristic carotenoid of Chrysophyceae and Dinophyceae, is observed.Chironomid analyses showed the near absence of a deep water fauna throughout the core length. The populations of chironomid larvae are restricted to oxygenated littoral zones. In fact, the few fossil remains found are primarily of littoral origin, representing shallow water midges that were transported to profundal waters. The reduction of total chironomid in the uppermost layers of the core is to be related to human land uses. 相似文献
14.
Microcoleus vaginatus isolated from a desert algal crust of Shapotou was cultured in BG-11 medium containing 0.2 mol l−1 NaCl or 0.2 mol l−1 NaCl plus 100 mg l−1 sucrose, extracellular polymeric substances (EPS) or hot water-soluble polysaccharides (HWP), respectively. Photosynthetic oxygen evolution rates, photosystem II activity (Fv/Fm) and dark respiration of NaCl-stressed cells were enhanced significantly by the added sucrose or EPS under salt stress conditions (0.2 mol l−1 NaCl). Compared with cells treated with salt alone, sodium contents in cells reduced significantly; the content of cellular total carbohydrate did not change, and intracellular sucrose, water-soluble sugar increased significantly following the addition of exogenous carbohydrates. Sucrose synthase (SS) activity of NaCl-stressed cells increased following the addition of sucrose, and sucrose phosphate synthase (SPS) activity of NaCl-stressed cells increased following the addition of exogenous sucrose, EPS or HWP compared with cells stressed with NaCl only. The results suggested that the extruded EPS might be re-absorbed by cells of M. vaginatus as carbon source, they could increase salt tolerance of M. vaginatus through the changes of carbohydrate metabolism and the selective uptake of sodium ions. 相似文献
15.
通过对商丘市1986—2000年烟草花叶病发病资料和气象资料的分析,找出了影响商丘市烟草花叶病发病的气象因子,并应用模糊数学原理建立了预报方程,在实际预报工作中应用效果较好。 相似文献
16.
17.
附着基粗糙度等因素与海藻密度的相关性研究 总被引:1,自引:0,他引:1
海藻附着基表面粗糙度与孢子附着密度有密切的相关性;已经由许多研究证实。但是海藻苗的密度是否仍然受到附着基粗糙度的影响;还缺乏明确的结论。本文以尼龙和亚克力两种材料作为海藻附着基;悬挂于自然海区中使海藻孢子附着并萌发生长;用以研究附着基表面粗糙度对4种海藻密度的影响。结果显示;多管藻、尾孢藻、点叶藻、硬毛藻这4种海藻;在不具沟槽的光滑面;密度显著低于任何有沟槽的粗糙面;但粗糙度不同的附着基之间比较;对海藻密度的影响并不显著。室内培育的海带苗;在尼龙上的密度与粗糙度成正相关;但是将海带苗移至海上培育后;不再与粗糙度有相关性;可能与其假根状固着器下海后的发育有关。本文还试验了海泥、灭活海泥和低栖硅藻3种沉积物与附着基粗糙度对海藻密度的影响;结果表明;沉积物和附着基粗糙度;以及它们的交互作用对海藻密度的影响;因附着基的材料和海藻种类而有差别。 相似文献
18.
铅锌矿是湘西-黔东地区的优势矿种,资源丰富,开发历史悠久,找矿潜力巨大。综合分析前人有关湘西-黔东生物礁的资料,介绍了藻灰岩的沉积特征,并结合大量实际地质资料,对藻灰岩控矿进行了论述。结果表明: 成矿物质主要来源于藻类及碳酸盐泥对Pb2+、Zn2+离子的吸取,矿床成因属于沉积成岩矿床类型,兼有成岩期后矿床性质; 藻礁灰岩与不同岩性的接合部位及其附近等微地球化学障区,往往就是铅锌富矿体产出部位; 藻礁灰岩与礁间通道的薄层泥质白云质灰岩呈指状交叉接触处,也常有铅锌富矿体产出; 铅锌富矿体基本上产于清虚洞组灰岩段中,且明显受该段藻礁灰岩控制。为满足国内外对铅锌矿日益增长的消费需要,深入研究区内铅锌矿的地层岩相岩石控矿因素及其富集规律,指导该地区藻灰岩中铅锌矿找矿工作,具有重要的现实意义。 相似文献
19.
White spot syndrome virus (WSSV) is one of the major shrimp pathogens causing large economic losses to shrimp farming. In an attempt to identify the envelope proteins involved in the virus infection, purified WSSV virions were mixed with three antisera against WSSV envelope proteins (VP39, VP124 and VP187 ), individually. And then they were injected intramuscularly into crayfish (Procambarus clarkii) to conduct in vivo neutralization assays. The results showed that for groups injected with virions only and groups injected with the mixture of virions and antiserum against VP124, the crayfish mortalities were 100% and 60% on the 8th day postinfection, individually. The virus infection could be delayed or neutralized by antibody against the envelope protein VP124. Quantitative PCR was used to further investigate the influence of three antisera described above on the virus infection. The results showed that the antiserum against VP124 could restrain the propagation of WSSV in crayfish. All of the results suggested that the viral envelope protein VP124 played a role in WSSV infection. 相似文献
20.
Phytoplankton dynamics associated with the monsoon in the Sulu Sea as revealed by pigment signature 总被引:2,自引:0,他引:2
Phytoplankton dynamics during the northeast monsoon was investigated in the Sulu Sea from algal pigment analysis. We visited
the Sulu Sea in February 2000, a mid period of the northeast monsoon, and in November and December 2002, the beginning of
the northeast monsoon. SeaWiFS images showed generally low concentrations of surface chlorophyll a (Chl a) during the southwest monsoon and higher concentrations with several peaks during the northeast monsoon. In the beginning
of the northeast monsoon, subsurface chlorophyll maxima (SCM) occurred, where vertical variation in class-specific composition
as estimated from pigment signatures was prominent. Prochlorococcus, cyanobacteria, prymnesiophytes and crysophytes were important groups above the SCM, and the contribution of cyanobacteria
to Chl a became much lower at and below the SCM. Contributions of chlorophytes and prasinophytes to Chl a generally showed maxima near the SCM. This distribution was accompanied by vertical changes in the concentration of photoprotective
pigments relative to photosynthetic accessory pigments. During the mid northeast monsoon, the upward supply of nutrients was
probably enhanced at some stations due to vertical mixing, and as a consequence diatoms dominated in the upper 100 m water
column of these stations, and other eukaryotic flagellates including prymnesiophytes, chrysophytes and cryptophytes were secondary
major components of the community. The elevation of Chl a concentration and changes in phytoplankton community during the northeast monsoon likely influence the variation in biological
production at higher trophic levels in the Sulu Sea. 相似文献