Post‐planktqnic stages of pinnotheres novaezelandiae filhol, 1886 (Brachyura: Pinnqtheridae)     

J. B. Jones 《新西兰海洋与淡水研究杂志》2013,47(1):145-158

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Decametric Response to the Great Impacts     

《紫金山天文台台刊》1996,(2)

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Polarimetry Of Comet Hale-Bopp: Linear And Circular Polarization, Stellar Occultation     

V. K. Rosenbush  N. M. Shakhovskoj  A. E. Rosenbush 《Earth, Moon, and Planets》1997,78(1-3):381-386

Comet Hale-Bopp was observed with the 2.6-m and 1.25-m telescopes of the Crimean Astrophysical Observatory on March 9 and 11, 1997. We determined the linear and circular polarization in the coma. For the dust coma, the linear polarization varied from 7.8% to 12.4%. The degree of circular polarization was always negative and did not exceed 0.3% with an accuracy of ±0.04% on average. The passage of a bright star through the cometary coma was monitored with polarimetry and photometry. The wavelength dependence and spatial variations of optical thickness of dust are obtained from the stellar occultation. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

Detecting genotoxicity using the Comet assay following chronic exposure of Manila clam Tapes semidecussatus to polluted estuarine sediments   总被引：1，自引：0，他引：1  

Coughlan BM  Hartl MG  O'Reilly SJ  Sheehan D  Morthersill C  van Pelt FN  O'Halloran J  O'Brien NM 《Marine pollution bulletin》2002,44(12):1359-1365

Sediments frequently cause damage to biota due to the accumulation of toxic compounds and the bioavailability of sediment-bound contaminants. Damage can be assessed using biomarkers, such as the degree of genotoxic impact following in vivo exposure to pollutants. Genotoxic damage, expressed as single-strand DNA breaks, was measured in cells isolated from haemolymph, gill and digestive gland from the clam Tapes semidecussatus, using the single cell gel electrophoresis (Comet assay). Clams were exposed for three weeks to sediment samples collected from a polluted site and a ‘clean’ reference site.

The level of DNA damage was assessed using an image analysis package and expressed as Tail Moment. Throughout the study, significant differences in DNA damage were recorded for each tissue type between clams exposed to the two sediment samples. We conclude that the Comet assay is a useful tool for the detection of DNA damage in clams chronically exposed to polluted sediments.  相似文献   

无锡梅村坠冰的研究     

胡中为  李兆麟 《高校地质学报》1997,3(4):361-369

１９８４年１１月１７日，从天空飞来一个冰块，坠落于锡山市（原无锡县）梅村乡陈三房村的麦田里（“梅村坠冰”）。现场和有关部门调查排除了人为成因，气象成因以及来自飞机的可能性。坠冰样品的实验分析研究的初步结果表明，它跟地球物质有差别，与地外物质相似，这有利于该坠冰来自无外的看法，或许可能来自慧星。坠洋的深入研究对于揭示慧星性质，探讨水以及有关地质，气象，天文问题可能有重大意义。  相似文献   

间接酶联免疫吸附技术检测活的非 可培养状态的大肠杆菌O157：H7   总被引：2，自引：0，他引：2  

姚斐  沙莎  陈刚  纪伟尚  徐怀恕 《中国海洋大学学报(自然科学版)》2001,31(2):211-214

大肠杆菌O157H7在低温贫营养的条件下可进 入活的非可培养状态（Viable but nonculturable state,VBNC），用常规的培养法无法将其检出。作者利用间接酶联免疫吸附技术（Indirect Enzyme-Linked Immunosorbent Assa y, ELISA）检测VBNC的大肠杆菌O157H7，发现此方法可以快速有效地将其检出，最小检测 浓度为10  相似文献   

1997年3月4日海尔-波普彗星的小规模分裂     

吴琴娣  赵爱娣  宋谊  张春生 《天文学报》2000,41(1):108-110

１　引言彗核分裂是彗星的最重要特征之一．至１９８２年止文献记录共观测到２２个彗核分裂,３３个次核［１］．１９８６年哈雷彗星回归时观测到彗核的分裂［２］．１９９６年紫金山天文台２００ｍｍ赤道望远镜和青岛观象台同时观测到百武彗星的分裂［３］．海尔波普彗星从１９９５年８月到１０月曾爆发５次［４］．１９９６年３月至１２月不断有喷流射出,彗核活动日趋剧烈［５］．１９９７年３月４日我们观测到彗核的小规模分裂及喷流特征,３月３日我们观测到海尔波普彗星近核照片上有喷流,这可能是３月４日彗核小块分裂的前奏．３…  相似文献   

Integration of the nuclease protection assay with sandwich hybridization (NPA-SH) for sensitive detection of Heterocapsa triquetra     

PARK Mirye  PARK So Yun  HWANG Jinik  JUNG Seung Won  LEE Juyun  CHANG Man  LEE Taek-Kyun 《海洋学报(英文版)》2018,37(5):107-112

Microalgae are photosynthetic microorganisms that function as primary producers in aquatic ecosystems. Some species of microalgae undergo rapid growth and cause harmful blooms in marine ecosystems. Heterocapsa triquetra is one of the most common bloom-forming species in estuarine and coastal waters worldwide. Although this species does not produce toxins, unlike some other Heterocapsa species, the high density of its blooms can cause significant ecological damage. We developed a H. triquetra species-specific nuclease protection assay sandwich hybridization (NPA-SH) probe that targets the large subunit of ribosomal RNA (LSU rRNA). We tested probe specificity and sensitivity with five other dinoflagellates that also cause red tides. Our assay detected H. triquetra at a concentration of 1.5×10⁴ cells/mL, more sensitive than required for a red-tide guidance warning by the Korea Ministry of Oceans and Fisheries in 2015 (3.0×10⁴ cells/mL). We also used the NPA-SH assay to monitor H. triquetra in the Tongyeong region of the southern sea area of Korea during 2014. This method could detect H. triquetra cells within 3 h. Our assay is useful for monitoring H. triquetra under field conditions.  相似文献   

生物固氮速率测定中15N₂同位素示踪剂的引入     

陈盛保  张润  陈能汪  赖丽旻  陈敏  郑敏芳  邱雨生 《海洋学报》2018,40(10):42-50

生物固氮作用是一个重要的海洋新氮来源，在海洋生物地球化学循环中扮演着不可替代的角色。基于稳定同位素（15N₂）示踪吸收法，是目前直接测定海洋生物固氮速率最有效的手段。其中，高效、洁净地将15N₂引入海水培养体系，并准确定量培养体系底物的同位素示踪水平，是同位素示踪吸收法准确获取固氮速率的关键。本研究针对15N₂同位素示踪剂引入这一关键环节进行了探讨，确认改进气泡法是将15N₂引入海水培养体系的首选操作。在对培养体系造成的较小扰动的情况下，可将培养体系氮气底物的15N原子丰度提升至10%以上，相对于另一种导入同位素示踪剂的手段——预溶解海水法，改进气泡法将培养瓶中氮气底物的15N原子丰度提升了近200%。此外，改进气泡法还具有最小化痕量金属沾污、操作简便等优点。将改进气泡法结合与稳定同位素比值质谱测定结合，是准确测定水体生物固氮速率的推荐方法。  相似文献   

聚苯乙烯微塑料与铜离子单一和复合污染对THP-1细胞的DNA损伤效应研究          下载免费PDF全文

尹艳  王海燕  韩大雄  林彩  林锡煌  单柏 《应用海洋学学报》2022,41(1):86-93

采用单细胞凝胶电泳实验研究了不同浓度的Cu²⁺(10、50、100、500、1000、2500、5000μg/L)和不同粒径(0.1、0.2、0.3、0.5、0.7、1.0、2.0μm)、不同浓度(1、10、15、20、25 mg/mL)的聚苯乙烯微塑料对人单核细胞白血病(Human Acute Monocytic Leukemia,THP-1)细胞的DNA损伤效应,结果表明:单一污染时,Cu²⁺(≥50μg/L)对THP-1细胞的DNA损伤极显著(p<0.01)。而且,DNA损伤值随Cu²⁺浓度的增大而增加。2.0μm粒径的聚苯乙烯微塑料(10 mg/mL)对THP-1细胞产生明显的DNA损伤效应。复合污染作用下对THP-1细胞的DNA损伤效应,相比单一污染明显增强,而且DNA损伤值比单一污染的理论加和值高。本研究表明Cu²⁺和聚苯乙烯微塑料均对DNA产生损伤效应,显示一定的基因毒性,二者复合对损伤具有交互增强的作用。  相似文献