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凡纳滨对虾咸淡水养殖系统内细菌群落组成的PCR-DGGE分析   总被引:16,自引:0,他引:16  
采用PCR(polym erase chain reaction)-DGGE(denaturing grad ient gel electrophoresis)及传统的微生物培养方法对凡纳滨对虾Litopenaeus vannam ei咸淡水养殖系统内各种环境的细菌群落组成进行了比较研究。传统的微生物培养计数表明,从海湾水、养殖池水到对虾粪样,细菌和弧菌的数量表现出依次增加的趋势,粪样及肠壁中弧菌的数量高出外界水环境1—4个数量级。相对于外界水环境,粪样中有很高的芽孢杆菌孢子含量,但是肠壁定植细菌中不存在芽孢杆菌(孢子)。PCR-DGGE及聚类分析结果表明,从海湾沿岸、养殖池、对虾粪便到对虾肠壁,细菌群落的多样性由高到低,无论是在哪种环境,群落的优势种都十分明显,且只有2—4种。来自同一环境各样品间的细菌群落组成非常相似,来自不同环境的样品,其细菌群落组成差别较大。聚类图上各簇的排列顺序反映了样品在取样空间分布上的毗邻次序和它们的相似程度。  相似文献   
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三亚湾浮游动物数量分布及群落特征的季节变化   总被引:4,自引:0,他引:4  
根据三亚湾2005年春、夏、秋和冬季航次的调查资料,分析了这4个季度的浮游动物的种类组成、优势种、群落结构、丰度和生物量的时空变化。经过鉴定共有终生性浮游动物125种,阶段性浮游动物17种,其中毛颚类幼虫和短尾溞状幼虫是各个季度都出现的优势种。三亚湾的浮游动物可以分为河口类群、暖水沿岸类群和广布暖水外海类群。全年浮游动物平均丰度为95.91ind·m-3,浮游动物平均生物量为69.22mg·m-3;浮游动物丰度的季节分布是夏>秋>春>冬,平均生物量的季节分布是秋>夏>春>冬,总体上一般是夏秋季高于冬春季。冬季浮游动物丰度与浮游植物细胞总密度之间没有相关性。  相似文献   
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Variation in the microbial biomass and community structure found in sediment of heavily polluted bays and the adjacent unpolluted areas were examined using phospholipid fatty acid analysis. Total microbial biomass and microbial community structure were responding to environmental determinants, sediment grain size, depth of sediment, and pollution due to petroleum hydrocarbons. The marker fatty acids of microeukaryotes and prokaryotes - aerobic, anaerobic, and sulfate-reducing bacteria -were detected in sediments of the areas studied. Analysis of the fatty acid profiles revealed wide variations in the community structure in sediments, depending on the extent of pollution, sediment depth, and sediment grain size. The abundance of specific bacterial fatty acids points to the dominance of prokaryotic organisms, whose composition differed among the stations. Fatty acid distributions in sediments suggest the high contribution of aerobic bacteria. Sediments of polluted sites were significantly enriched with anaerobic bacteria in comparison with clean areas. The contribution of this bacterial group increased with the depth of sediments. Anaerobic bacteria were predominantly present in muddy sediments, as evidenced from the fatty acid profiles. Relatively high concentrations of marker fatty acids of sulfate-reducing bacteria were associated with organic pollution in this site. Specific fatty acids of microeukaryotes were more abundant in surface sediments than in deeper sediment layers. Among the microeukaryotes, diatoms were an important component. Significant amounts of bacterial biomass, the predominance of bacterial biomarker fatty acids with abundance of anaerobic and sulfate-reducing bacteria are indicative of a prokaryotic consortium responsive to organic pollution.  相似文献   
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Vertical distribution (0–15 cm) of the macrobenthic community and its relationships to natural sediment characteristics and trace metal contents and bioavailability were studied at five locations in the lower Douro estuary, Portugal. An analysis of vertical metal distribution, for the interpretation of anthropogenic impact on the estuarine sediments, was also investigated. Sediment characterisation included organic matter, grain size, metals (Al, Fe, Cu, Pb, Cr, Ni, Cd, Zn and Mn), acid volatile sulphide (AVS) and simultaneously extracted metals (SEM). The macrobenthic community had low diversity (14 species), was dominated by small size opportunists and seemed to be controlled mainly by natural factors such as grain size distribution, Al and Fe contents and sediment depth. The vertically heterogeneous distribution of macrobenthic community appears to affect redox status of the sediments and consequently metal bioavailability. Despite anthropogenic contamination in terms of Zn, Cu, Pb, Cr and Ni having already been detected in the north bank, the analysis of vertical distribution was essential for the identification of current anthropogenic contamination in terms of Zn, Pb and Cd in the south bank.  相似文献   
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2001年夏季胶州湾浮游植物群落结构的特征   总被引:7,自引:2,他引:7  
本文根据 2 0 0 1年 8月 2 0~ 2 2日期间对胶州湾海域进行的大面积调查资料 ,对浮游植物的群落结构进行了初步研究。结果表明 ,浮游植物群落主要由沿岸暖水性种类组成 ,以硅藻为主 ,还有少量的甲藻、金藻和绿藻。湾中央出现的种类最多 ,湾口其次 ,湾边缘最少。细胞数量的高值区出现在B断面和 A5站周围的水域 ,低值区出现 D断面和 E断面。此外 ,对群落多样性的研究表明 ,Shannon指数在湾北部的多数站位高于湾南部水域 ,在 D2和 B1站出现 2个低值区 ;Margalef指数在湾内东部和 D4站周围水域明显高于湾内西部和湾口水域 ;均匀度则在 B1和 D2站周围水域偏高。浮游植物群落同营养盐的相关分析表明 ,调查期氨盐和磷酸盐是影响浮游植物生长的主要营养盐 ,中肋骨条藻、浮动弯角藻和旋链角毛藻等几种优势种类决定了这一关系  相似文献   
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Abstract. Vacated microborings in periostracum of live mussels of the Florida Escarpment redox community (depth of 3266 m) become a habitat for a prolific secondary microbiota consisting of a variety of prokaryotic and a few eukaryotic organisms. Periostracum surface is also colonized by diverse microorganisms, dominated by presumed chemolithotrophic bacteria with stacks of intracellular lamellae. Unlike sheltered microflora within borings, the surface community is heavily grazed upon by numerous archaeogastropods and ciliates.  相似文献   
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Abstract. Aquatina lake is a brackish basin, connected with the Adriatic Sea along the mast of Apulia (Italy), with a mean salinity of 26%. The abundance and biomass of fifteen polychaete species were recorded by monthly samples in a pilot area of the lake from February 1989 to February 1990. Naineris laevigata was dominant, both in number of individuals and biomass. During 1990, obstruction of freshwater inflow to the lake caused an increase in salinity up to 4O%, followed by some changes in the polychaete community. Noromasrus hiericeus became dominant, and the abundances of the other species decreased except for Naineris laevipru . Salinity assumed normal values after three months, but community responded slowly becauie the massive presence of N. latericeus inhibited the recovery of other species. An abiotic disturbance, followed by a biotic disturbance, altered the structure of the polychaete community.  相似文献   
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