Cloning of Na~+/H~+ antiport gene from Dunaliella salina     

KONG Fanjing  CHEN Susu 《《地质学报》英文版》2014,88(Z1):78-79

正1 Introduction Dunaliella Salina,which taxi Dunaliella,Volvocales,Chlorophyceae Chlorophyta,is unicell algae with double flagllum at top,and cup shaped chloroplast without cell wall.Dunaliella Salina is the most salt tolerance eucaryotes.It can grow at the range of salt concentration  相似文献   

Screening and molecular classification of low-temperature protease from Antarctic microorganism and its characterization     

王全富缪锦来  侯艳华郑洲 李光友 《极地研究(英文版)》2005,16(2):81-89

107 strains producing protease were screened from 260 strains of Antarctic psychrophilic bacteria, among which proteolytic activity of five strains was more than 45 U ml^-1. The 16S rRNA gcne sequences homology and phylogcnetic analysis of five Antarctic psychrophillc bacteria showed that NJ276, NJS-9, NJ16-70,NJ345 belonged tO the described genus Pseudoalteromonas and NJ341 belonged to the genus Colwellia. The growth and the protease characteristic of four Antarctic psychrophilic bacteria had been studied, and the result showed that the 6ptimal temperature for growth and protease-produeing of four strains was about 10℃. Their growth and protease-produeing were still high during incubatlng 2-5 days. The maximum proteolytic activity occurred at pH 9 for four Antarctic psychrophilic bacteria. The optimal temperature of protease action of both strains NJ276 and NJ5-9 was about 50℃, however, the optimal temperature of protease aetlon of both strains NJ341 and NJ345 was about 40 ℃, and their proteolytic activity under 0℃ exhibited nearly 30% of the maximum activity, but their thermal stabilities were weaker. These results indicated that proteases from NJ341 and NJ345 were low-temperature proteases.  相似文献   

Transformation of ammonium nitrogen and response characteristics of nitrifying functional genes in tannery sludge contaminated soil          下载免费PDF全文

Kong Xiang-ke  Zhang Zi-xuan  Wang Ping  Wang Yan-yan  Zhang Zhao-ji  Han Zhan-tao  Ma Li-sha 《地下水科学与工程》2022,10(3):223-232

High concentrations of ammonium nitrogen released from tannery sludge during storage in open air may cause nitrogen pollution to soil and groundwater. To study the transformation mechanism of NH₄⁺-N by nitrifying functional bacteria in tannery sludge contaminated soils, a series of contaminated soil culture experiments were conducted in this study. The contents of ammonium nitrogen (as NH₄⁺-N), nitrite nitrogen (as NO₂^?-N) and nitrate nitrogen (as NO₃^?-N) were analyzed during the culture period under different conditions of pollution load, soil particle and redox environment. Sigmodial equation was used to interpret the change of NO₃^?-N with time in contaminated soils. The abundance variations of nitrifying functional genes (amoA and nxrA) were also detected using the real-time quantitative fluorescence PCR method. The results show that the nitrification of NH₄⁺-N was aggravated in the contaminated silt soil and fine sand under the condition of lower pollution load, finer particle size and more oxidizing environment. The sigmodial equation well fitted the dynamic accumulation curve of the NO₃^?-N content in the tannery sludge contaminated soils. The Cr(III) content increased with increasing pollution load, which inhibited the reproduction and activity of nitrifying bacteria in the soils, especially in coarse-grained soil. The accumulation of NO₂^?-N contents became more obvious with the increase of pollution load in the fine sand, and only 41.5% of the NH₄⁺-N was transformed to NO₃^?-N. The redox environment was the main factor affecting nitrification process in the soil. Compared to the aerobic soil environment, the transformation of NH₄⁺-N was significantly inhibited under anaerobic incubation condition, and the NO₃^?-N contents decreased by 37.2%, 61.9% and 91.9% under low, medium and high pollution loads, respectively. Nitrification was stronger in the silt soil since its copy number of amoA and nxrA genes was two times larger than that of fine sand. Moreover, the copy numbers of amoA and nxrA genes in the silt soil under the aerobic environment were 2.7 times and 2.2 times larger than those in the anaerobic environment. The abundance changes of the amoA and nxrA functional genes have a positive correlation with the nitrification intensity in the tannery sludge-contaminated soil.  相似文献   

Time‐series high‐resolution spectroscopy and photometry of ε Aurigae from 2006–2013: Another brick in the wall     

K. G. Strassmeier  M. Weber  T. Granzer  L. Schanne  J. Bartus  I. Ilyin 《Astronomische Nachrichten》2014,335(9):904-934

We present continuous and time‐resolved R = 55 000 optical échelle spectroscopy of ε Aurigae from 2006–2013. Data were taken with the STELLA Echelle Spectrograph of the robotic STELLA facility at the Observatorio del Teide in Tenerife. Contemporaneous photometry with the Automatic Photoelectric Telescopes at Fairborn Observatory in Arizona is presented for the years 1996–2013. Spectroscopic observations started three years prior to the photometric eclipse and are still ongoing. A total of 474 high‐resolution échelle spectra are analyzed and made available in this paper. We identify 368 absorption lines of which 161 lines show the characteristic sharp disk lines during eclipse. Another 207 spectral lines appeared nearly unaffected by the eclipse. From spectrum synthesis, we obtained the supergiant atmospheric parameters T_eff = 7395 ± 70 K, log g ≈ 1, and [Fe/H] = +0.02 ± 0.2 with ξ_t = 9 km s^–1, ζ_RT = 13 km s^–1, and v sin i = 28 ± 3 km s^–1. The residual average line broadening expressed in km s^–1 varies with a period of 62.6 ± 0.7 d, in particular at egress and after the eclipse. Two‐dimensional line‐profile periodograms show several periods, the strongest with ≈110 d evident in optically thin lines as well as in the Balmer lines. Center‐of‐intensity weighted radial velocities of individual spectral lines also show the 110‐d period but, again, additional shorter and longer periods are evident and are different in the Balmer lines. The two main spectroscopic Hα periods, ≈ 116 d from the line core and ≈ 150 d from the center‐of‐intensity radial velocities, appear at 102 d and 139 d in the photometry. The Hβ and Johnson V I photometry on the other hand shows two well‐defined and phase‐coherent periods of 77 d and 132 d. We conclude that Hα is contaminated by changes in the circumstellar environment while the Hβ and V I photometry stems predominantly from the non radial pulsations of the F0 supergiant. We isolate the disk‐rotation profile from 61 absorption lines and found that low disk eccentricity generally relates to low disk rotational velocity (but not always) while high disk eccentricity always relates to high velocity. There is also the general trend that the disk‐absorption in spectral lines with higher excitation potential comes from disk regions with higher eccentricity and thus also with higher rotational velocity. The dependency on transition probability is more complex and shows a bi‐modal trend. The outskirts of the disk is distributed asymmetrically around the disk and appears to have been built up mostly in a tail along the orbit behind the secondary. Our data show that this tail continues to eclipse the F0 Iab primary star even two years after the end of the photometric eclipse. High‐resolution spectra were also taken of the other, bona‐fide, visual‐binary components of ε Aur (ADS 3605BCDE). Only the C‐component, a K3‐4‐giant, appears at the same distance than ε Aur but its radial velocity is in disagreement with a bound orbit. The other components are a nearby (≈ 7 pc) cool DA white dwarf, a G8 dwarf, and a B9 supergiant, and not related to ε Aur. The cool white dwarf shows strong DIB lines that suggest the existence of a debris disk around this star. (© 2014 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)  相似文献   

Symbiotic stars with spectral indication of bipolar ejection and stellar wind     

N.A. Tomov  M.T. Tomova  D.V. Bisikalo 《Astronomische Nachrichten》2014,335(2):178-188

Four out of a total of five symbiotic systems whose optical spectral lines contain satellite components indicating collimated ejection have an additional absorption of P Cyg type with different velocity. These systems are Z And, Hen 3‐1341, StHα 190, and BF Cyg. It is shown that the line profiles of every of these systems can be interpreted in the framework of a model of a collimated stellar wind from the hot compact component proposed initially to interpret the line profiles of Z And, observed during its last active phase after 2000. (© 2014 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)  相似文献   

Bulk organic δ13C and C/N ratios as palaeosalinity indicators within a Scottish isolation basin     

Elizabeth A. V. Mackie  Melanie J. Leng  Jeremy M. Lloyd  Carol Arrowsmith 《第四纪科学杂志》2005,20(4):303-312

Microfossils in isolation basin sediments are frequently used to reconstruct sea‐level change, but preservation problems and non‐analogue situations can limit their usefulness. Here we investigate the potential of stable carbon isotopes (δ¹³C) and C/N ratios from bulk organic matter, as an alternative proxy of salinity within isolation basin sediments from a basin in northwest Scotland. Within the Holocene sediment δ¹³C and C/N are determined largely by the mean weighted values of the predominant source of the organic material. Analysis of modern materials and comparison with the diatom record shows that the marine parts of the sequence are dominated by high δ¹³C and variable C/N. In the fresh water sequences the organic material is a mixture of both freshwater aquatic and terrestrial plant input that have relatively low δ¹³C and high C/N. The application of δ¹³C and C/N ratios in the studied basin in general follow the environmental change recorded by the diatoms and shows the potential of bulk organic matter in the investigation of salinity change in isolation basins. Copyright © 2005 John Wiley & Sons, Ltd.  相似文献   

一株H_5N_1亚型禽流感病毒株NA基因的克隆与序列分析     

陈绍红  刘艳芬  张静  刘铀 《广东海洋大学学报》2007,27(4):85-88

用RT-PCR方法从1个H5N1亚型禽流感病毒分离株A/Chicken/Guangdong/DH/1997扩增NA基因cDNA片段,将其克隆至pMD18-T载体,获得重组质粒pMD-NA,并对其核苷酸序列进行测定和分析。结果表明,该毒株的NA基因长度为1350bp,编码449个氨基酸,与其它H5N1亚型AIV分离株的核苷酸序列同源性为97.0%～99.4%,氨基酸序列同源性为97.7%～99.1%,提示禽流感病毒NA基因保守性较高。NA基因氨基酸序列的聚类分析表明该毒株与来自香港的A/Pheasant/HK/FY155/01和A/Ch/HK/FY150/01两个分离株处于同一进化枝,亲缘关系较近。  相似文献   

An integrated linear/non-linear flow model for the conduit-fissure-pore media in the karst triple void aquifer system   总被引：2，自引：0，他引：2  

J. M. Cheng  C. X. Chen 《Environmental Geology》2005,47(2):163-174

Most karstic aquifer media may be characterized as the triple-void media with highly-varied hydraulic properties, including matrix pore, fissure and conduit, in which liner flow may co-exist with non-linear flow. In this paper, an attempt is made to couple linear flow with non-linear flow in a single unified flow governing equations by introducing the concept of equivalent hydraulic conductivity (EHC) and deriving a general Darcys law for various flow. The expression of EHC in the karst conduit and fissure are also derived. The procedures of numerical implementation are demonstrated via an ideal model and a case study of karst aquifer system in the Beishan Ore Formation area, Guangxi Autonomous Region, China.  相似文献   

Unstable kinetic Alfvén wave in partially ionized plasma     

J. Vranjes  D. Petrovic  M. Kono 《Planetary and Space Science》2006,54(6):641-644

The stability of kinetic Alfven waves is discussed for a partially ionized plasma with a flux of ionizing electrons which balance the plasma particle losses. Accidental electromagnetic perturbations are shown to be unstable due to the energy change of ionizing electrons.  相似文献   

New magnetic field measurements of β Cephei stars and slowly pulsating B stars     

S. Hubrig  M. Briquet  P. De Cat  M. Schller  T. Morel  I. Ilyin 《Astronomische Nachrichten》2009,330(4):317-329

We present the results of the continuation of our magnetic survey with FORS 1 at the VLT of a sample of B‐type stars consisting of confirmed or candidate β Cephei stars and Slowly Pulsating B (hereafter SPB) stars, along with a small number of normal B‐type stars. A weak mean longitudinal magnetic field of the order of a few hundred Gauss was detected in three β Cephei stars and two stars suspected to be β Cephei stars, in five SPB stars and eight stars suspected to be SPB stars. Additionally, a longitudinal magnetic field at a level larger than 3σ has been diagnosed in two normal B‐type stars, the nitrogen‐rich early B‐type star HD 52089 and in the B5 IV star HD 153716. Roughly one third of β Cephei stars have detected magnetic fields: Out of 13 β Cephei stars studied to date with FORS 1, four stars possess weak magnetic fields, and out of the sample of six suspected β Cephei stars two show a weak magnetic field. The fraction of magnetic SPBs and candidate SPBs is found to be higher: Roughly half of the 34 SPB stars have been found to be magnetic and among the 16 candidate SPBs eight stars possess magnetic fields. In an attempt to understand why only a fraction of pulsating stars exhibit magnetic fields, we studied the position of magnetic and non‐magnetic pulsating stars in the H‐R diagram. We find that their domains in the H‐R diagram largely overlap, and no clear picture emerges as to the possible evolution of the magnetic field across the main sequence. It is possible that stronger fields tend to be found in stars with lower pulsating frequencies and smaller pulsating amplitudes. A somewhat similar trend is found if we consider a correlation between the field strength and the v sin i ‐values, i.e. stronger magnetic fields tend to be found in more slowly rotating stars (© 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)  相似文献