不同培养条件对三角褐指藻生长及其生物活性成分积累的影响   总被引：1，自引：0，他引：1  

张文源  高保燕  李爱芬  张成武 《海洋科学》2016,40(5):57-65

以三角褐指藻(Phaeodactylum tricornutum)为研究材料,比较了Na NO3、NH4HCO3和CO(NH2)2为氮源的两种培养基(m L1和ASW培养基)对其生长和生物活性成分(岩藻黄素、金藻昆布糖和二十碳五烯酸(C20:5,EPA))时相积累的影响,同时分析了脂肪酸组成和总脂含量的变化。结果表明:以m L1培养基培养时,三角褐指藻的生物质质量浓度明显高于ASW培养基培养时的生物质质量质量浓度,尿素优于其他两种氮源,最大生物质质量质量浓度为3.7 g/L。不同培养条件下岩藻黄素含量的时相变化规律一致,均随着培养时间的延长呈现先增加后减少的趋势,其最高积累量分别为:13.27 mg/g(Na NO3)、13.23 mg/g(CO(NH2)2)和13.89 mg/g(NH4HCO3)(m L1);13.2 mg/g(Na NO3)、14.92 mg/g(CO(NH2)2)和13.6 mg/g(NH4HCO3)(ASW),由此可知氮源对岩藻黄素积累量影响不大。金藻昆布糖含量随着培养时间延长逐渐增加,其最大积累量分别为9.82 mg/g(NH4HCO3)(m L1)和8.59 mg/g(Na NO3)(ASW)。不同培养条件下其总脂含量变化不显著,均在培养末期达到最大值,分别为24.18%(NH4HCO3)(m L1)和23.79%(Na NO3)(ASW);其主要脂肪酸组成为:豆蔻酸(C14:0)、棕榈酸(C16:0)、棕榈油酸(C16:1)、硬脂酸(C18:0)、油酸(C18:1)、亚油酸(C18:2)、花生一烯酸(C20:1)、木焦油酸(C24:0)和EPA,其中,EPA含量随着培养时间延长逐渐下降,尿素最有利于EPA的积累。  相似文献   

西菲律宾海2万年以来浮游有孔虫壳体重量变化     

安佰正  李铁刚  孙晗杰  于心科  仇晓华 《海洋科学》2016,40(5):115-120

为探讨2万年以来浮游有孔虫的壳体重量变化及其影响因素,对取自西菲律宾海MD06-3052岩芯的浮游有孔虫表层种Globigerinoides ruber进行壳体重量分析,基于氧同位素年龄模式,获得了2万年以来有孔虫传统壳体重量(WMS)和标准化壳体重量(WSN)变化特征。对比两种壳体重量指标,发现在本海区标准化壳体重量指标更为准确。结果表明,2万年以来,浮游有孔虫WSN具有不断变轻的趋势,整体表现为冰期时壳体重量较重,而全新世时壳体重量相对较轻的特征。末次冰盛期(Last Glacial Maximum,LGM)以来浮游有孔虫WSN变化曲线同大气CO2浓度变化曲线具有较好的相似性,推测在该海区控制壳体重量的主要因素为与全球大气CO2相关的表层海水的CO32–浓度。  相似文献   

Effects of heavy metal (Pb) concentration on some growth parameters of plants grown in lead polluted soil under organic fertilizer amendment     

Ojo M. Oseni  Adekunle A. Adelusi  Esther O. Da  Abdulfatai B. Rufai 《寒旱区科学》2016,8(1):36-45

This study investigated morphological variation and biomass accumulation that occurred in Sida acuta and Chromolaena odorata plants grown in lead polluted soil under organic fertilizer amendment. The study was carried out in the screen house at the Biological Gardens of the Obafemi Awolowo University, Ile-Ife, Osun State. The experiment was a factorial combination of one heavy metal (Pb) at five levels of concentration (0, 200, 400, 800 and 1,000 mg/kg) in a completely randomized design, and were replicated three times for each of the two plants and two levels (0 g/kg and 9.4 g/kg) of organic fertilizer (OBD-Plus). Each pot was filled with 5 kg of air-dried and sieved soil and placed on a plastic tray for the collection of excess water. Two weeks after planting, seedlings of uniform height were transplanted from the nursery to experimental pots at the rate of one seedling per pot and grown for 10 weeks. The growth parameters of the plants were biomonitored for 7 weeks. After 10 weeks of treatment, the plants were harvested and dried to calculate the biomass accumulation. The two plant species performed better under fertilizer application than without it. For each of the plant species the growth parameters decreased as the levels of Pb concentration increased. Furthermore, the plants' biomass decreased significantly (p<0.05) as the levels of Pb concentration increased. The organic fertilizer helped to improve the plants' performance in lead-polluted soil.  相似文献   

Diagnosis of earth-fill dams by synthesised approach of sounding and surface wave method     

Shin-ichi Nishimura  Toshifumi Shibata  Takayuki Shuku 《Georisk: Assessment and Management of Risk for Engineered Systems and Geohazards》2016,10(4):312-319

The spatial distribution of the strength inside the earth-fill is identified by the sounding tests. In this research, the Swedish weight sounding (SWS) is employed, and the spatial high-density test is performed to identify the spatial correlation structure. Furthermore, the synthesised approach of the SWS and surface wave method, which is one of the geophysical method, is proposed to compensate the shortage of each approach. Consequently, the correlation structure of an earth-fill could be identified accurately, and the high resolution of the spatial distribution could be visualised based on the survey results.  相似文献   

三峡库岸滑坡变形特征及影响因素分析          下载免费PDF全文

李远宁  潘勇  冯晓亮  陈龙  程奎 《探矿工程》2018,45(8):127-131

三峡库区涉水滑坡主要影响因素是水位和降雨量,也是库区滑坡体失稳的主要影响因素和诱发因素。库区每年重复着水位升降不利于滑坡的稳定,而降雨特别是大强度的降雨也诱发产生滑坡。当水位波动遇到降雨,出现工况叠加,滑坡将加剧。因此,有必要对影响滑坡变形的主导因素进行了解分析。2016年6月三峡库区全面展开了自动化监测,使得数据统计方便可靠。本文采用滑坡变形速率、降雨量、库水位变化、最大水位变化速率、淹没程度,运用灰色关联度分析法对涉水滑坡进行了计算分析。水位下降阶段,文中土质滑坡变形受库水位影响最大。水位上升阶段,该土质滑坡上部变形受降雨影响最大,下部受水位影响最大。文中岩质滑坡总是受库水位影响最大。  相似文献   

The geothermal formation mechanism in the Gonghe Basin: Discussion and analysis from the geological background     

《China Geology》2018,1(3):331-345

The Gonghe Basin, a Cenozoic down-warped basin, is located in the northeastern part of the Qinghai-Xizang (Tibetan) Plateau, and spread over important nodes of the transfer of multiple blocks in the central orogenic belt in the NWW direction. It is also called “Qin Kun Fork” and “Gonghe Gap”. The basin has a high heat flow value and obvious thermal anomaly. The geothermal resources are mainly hot dry rock and underground hot water. In recent years, the mechanism of geothermal formation within the basin has been controversial. On the basis of understanding the knowledge of predecessors, this paper proposes the geothermal formation mechanism of the “heat source–heat transfer–heat reservoir and caprock–thermal system” of the Gonghe Basin from the perspective of a geological background through data integration-integrated research-expert, discussion-graph, compilation-field verification and other processes: (1) Heat source: geophysical exploration and radioisotope calculations show that the heat source of heat in the basin has both the contribution of mantle and the participation of the earth’s crust, but mainly the contribution of the deep mantle. (2) Heat transfer: The petrological properties of the basin and the exposed structure position of the surface hot springs show that one transfer mode is the material of the mantle source upwells and invades from the bottom, directly injecting heat; the other is that the deep fault conducts the deep heat of the basin to the middle and lower parts of the earth’s crust, then the secondary fracture transfers the heat to the shallow part. (3) Heat reservoir and caprock: First, the convective strip-shaped heat reservoir exposed by the hot springs on the peripheral fault zone of the basin; second, the underlying hot dry rock layered heat reservoir and the upper new generation heat reservoir and caprock in the basin revealed by drilling data. (4) Thermal system: Based on the characteristics of the “heat source-heat transfer-heat reservoir and caprock”, it is preliminarily believed that the Gonghe Basin belongs to the non-magmatic heat source hydrothermal geothermal system (type II2₁) and the dry heat geothermal system (type II2₂). Its favorable structural position and special geological evolutionary history have given birth to a unique environment for the formation of the geothermal system. There may be a cumulative effect of heat accumulation in the eastern part of the basin, which is expected to become a favorable exploration area for hot dry rocks.  相似文献   

5 000 m深部干热岩钻井方案的技术经济评价   总被引：1，自引：1，他引：0       下载免费PDF全文

赵远刚 《中国地质调查》2018,5(2):73-77

介绍了美国芬顿山和法国苏茨深部干热岩的典型钻井技术方案,并根据我国干热岩钻井基础和地质成藏条件,从钻井深度、井身结构、钻进工艺和钻机设备等方面制定了3种不同的干热岩钻井技术方案,给出了具体技术指标数值。通过计算,对每种方案进行了技术经济评价,结果认为: 大直径钻井方案是技术可靠、经济可行的5 000 m干热岩钻探施工方案。  相似文献   

全国地热资源调查评价与勘查示范工程进展   总被引：2，自引：0，他引：2       下载免费PDF全文

王贵玲  张薇  蔺文静  刘峰  甘浩男  付雷 《中国地质调查》2018,5(2):1-7

地热作为一种清洁能源,在我国经济发展与能源转型过程中正发挥着越来越重要的作用。“全国地热资源调查评价与勘查示范工程”自实施以来,主要开展了京津冀地区地热资源评价以及东南沿海和青藏高原东部干热岩的勘查等工作。这些工作的开展在获得一系列科研突破成果的同时,也解决了大量地热开发的工程问题,积累了大量成果和经验。该文对工程取得的进展及成果进行了总结,对工作开展中出现的问题进行了探讨,可用于指导我国地热资源勘查开发工作。  相似文献   

KT178型取心钻具在共和干热岩钻井中的应用          下载免费PDF全文

张恒春  王稳石  李宽  王跃伟  闫家  曹龙龙  胡晨 《探矿工程》2021,48(2):29-34

共和干热岩资源富集于花岗岩地层,硬度高,研磨性强,部分层段裂隙发育,取心困难。针对干热岩地层和取心需求特点,研制一套KT178取心钻具,并配套设计了孕镶金刚石取心钻头。该钻具为单动双管取心钻具,可配套井底动力钻具使用,设计中注重对钻具外管、卡簧等关键部件的强度校核。在GH-01井和GH-02井中,经过两轮实验和优化,钻具逐渐成熟,获取了较完整的岩心样本,满足干热岩勘探取心要求,具备了向页岩气、水资源勘察等具有间断取心需求的同类工程推广应用的条件。  相似文献   

高温硬岩空气潜孔锤钻头设计          下载免费PDF全文

罗宏保  李俊萍  吴金生 《探矿工程》2021,48(4):60-65

干热岩试验性开发井钻井施工,在高温硬岩的地层条件下,如何实现高效钻进仍存在很多技术难点。空气潜孔锤钻进技术是解决硬岩地层钻进的有效方法之一,潜孔锤钻头是空气潜孔锤钻进的关键器具。根据青海共和干热岩GH-01井空气潜孔锤钻进技术应用情况,分析探讨了空气潜孔锤钻头断齿、掉齿、磨损严重、使用寿命短等问题,从钻头结构设计、钻头材料选取、钻头冷压固齿工艺等几个方面对空气潜孔锤钻头进行了优化。固齿优化过程中采用有限元分析方法,对钻头冷压固齿过盈量进行仿真分析计算,根据分析计算结果确定最优固齿过盈量。通过对潜孔锤钻头结构、材料、加工等优化,为适用于高温硬岩的空气潜孔锤钻头研制提供了借鉴。为提高空气潜孔锤钻进综合效率,为干热岩钻探开发提供了技术支持和技术储备。  相似文献