起始pH值对海洋混合菌群产氢量的影响     

刘洪艳  王广策 《海洋科学》2011,35(9):43-48

对采自天津潮间带的污泥进行热休克处理,厌氧条件下富集混合菌群进行产氢试验;设置起始pH从4．0到8．0（间隔0．5个单位）,比较混合菌群在不同pH培养条件下产氢量;利用变性梯度凝胶电泳（DGGE）分析不同起始pH培养条件下的混合菌群组成。结果表明,在起始pH为7．0时,混合菌群产氢量最高。不同起始pH对产氢量的影响,依...  相似文献   

高效毛细管电泳法测定海参多糖的单糖组成   总被引：1，自引：0，他引：1  

杨洁  姜廷福  王远红  吕志华 《中国海洋大学学报(自然科学版)》2011,(Z1):344-348

目的:采用高效毛细管电泳法测定3种不同海参多糖的单糖组成。方法:海参多糖水解后,经PMP衍生化,高效毛细管电泳分析。电泳条件:缓冲液为60 mmol.L-1硼砂溶液(pH=9.0);柱温25℃;运行电压25 kV;检测波长245 nm;压力进样50 mbar,进样时间5 s。结果:3种不同海参多糖中均含GalN、GlcN、GalNAC、Glc、Man、Fuc、Gal、GlcUA,梅花参多糖中各单糖的摩尔比为1.00∶1.02∶1.63∶1.19∶1.02∶10.26∶0.82∶0.38,四方刺参多糖中各单糖的摩尔比为1.00∶0.41∶0.43∶0.56∶0.29∶5.47∶0.43∶0.25,北极参多糖中各单糖的摩尔比为1.00∶0.26∶0.22∶0.25∶0.06∶1.36∶0.27∶0.16。结论:该方法具有简单、快速、分离效率高等特点,能同时测定海参多糖中氨基糖、中性糖、糖醛酸的组成。  相似文献   

Analysis of community structure of a microbial consortium capable of degrading benzo(a)pyrene by DGGE     

Y.R. Luo  Y. Tian  X. Huang  H.S. Hong  T.L. Zheng 《Marine pollution bulletin》2009,58(8):1159-1163

A microbial consortium was obtained by enrichment culture of sea water samples collected from Botan oil port in Xiamen, China, using the persistent high concentration of a mixture of polycyclic aromatic hydrocarbons enrichment strategy. Denaturing gradient gel electrophoresis (DGGE) was used to investigate the bacterial composition and community dynamic changes based on PCR amplification of 16S rRNA genes during batch culture enrichment. Using the spray-plate method, three bacteria, designated as BL01, BL02 and BL03, which corresponded to the dominant bands in the DGGE profiles, were isolated from the consortium. Sequence analysis showed that BL01, BL02 and BL03 were phylogenetically close to Ochrobactrum sp., Stenotrophomonas maltophilia and Pseudomonas fluorescens, respectively. The degradation of benzo(a)pyrene (BaP), a model high-molecular-weight polycyclic aromatic hydrocarbon (HMW PAH) compound was investigated using individual isolates, a mixture of the three isolates, and the microbial consortium (BL) originally isolated from the oil port sea water. Results showed that the order of degradative ability was BL > the mixture of the three isolates > individual isolates. BL degraded 44.07% of the 10 ppm BaP after 14 days incubation, which showed the highest capability for HMW PAH compound degradation.Our results revealed that this high selective pressure strategy was feasible and effective in enriching the HMW PAH-degraders from the original sea water samples.  相似文献   

魁蚶(Scapharca broughtonii)等位基因酶遗传变异研究   总被引：1，自引：0，他引：1  

喻子牛  孔晓瑜  杨锐  陈再忠  刘必谦  王如才 《中国海洋大学学报(自然科学版)》1998,(1)

采用水平淀粉凝胶电泳技术研究了秦皇岛、大连、青岛和韩国釜山４个魁蚶群体样本的等位基因酶遗传变异。在四个群体的１０～１２种等位基因酶中分别检测到了２２、２６、２２和２７个基因位点,４个群体的多态位点比例（Ｐ０．９９）分别为５０．００、５７．６９、４５．５０和６２．９６％,平均杂合度（观察值）则分别为０．０８７±０．０２４、０．１２３±０．０３８、０．１０５±０．０２３和０．０９１±０．０３１;平均位点有效等位基因数分别是１．２３０、１．３９８、１．４１５和１．２５３。另外,计算４个群体之间的遗传相似度（Ⅰ）和遗传距离（ＤＮｅｉ）,表明遗传同一性以秦皇岛和大连群体之间最大,其次为秦皇岛与青岛及大连与青岛群体之间,韩国釜山与其它３个群体之间存在一定的遗传分岐。４个群体在多个位点上都存在明显的杂合子缺失现象。文中初步探讨了引起杂合子缺失的可能原因  相似文献   

毛细管电泳法测定芦根和蒲黄多糖中的单糖组分(英文)     

于玮洁  姜庭福  王远红  吕志华 《海洋湖沼通报》2010,(2)

本文建立了用毛细管电泳测定中草药芦根和蒲黄多糖中的单糖组分的方法。提取的单糖经α-萘胺衍生,用二极管阵列检测器在214nm处检测。五种单糖鼠李糖(Rha),木糖(Xyl),半乳糖(Gal),葡萄糖(Glu),阿拉伯糖(Ara)能够同时测定,分离良好。毛细管电泳的测定条件如下,50 mM硼酸钠缓冲液;pH10.5;工作电压为25kV;温度为25℃。标准曲线的线性范围为5 mg/L-200 mg/L,每种单糖的最低检测线均为2.5 mg/L。迁移时间和峰面积的相对标准偏差分别小于1.46%,6.21%。实验结果显示,芦根多糖重各单糖组分的摩尔比如下:木糖∶葡萄糖∶阿拉伯糖∶半乳糖为1.00∶59.03∶2.09∶1.96;蒲黄多糖中各单组分的摩尔比如下:鼠李糖∶木糖∶葡萄糖∶阿拉伯糖∶半乳糖为1.71∶1.00∶9.30∶6.88∶1.24。  相似文献   

毛细管电泳在线富集技术研究新进展   总被引：1，自引：0，他引：1  

燕娜  陈兴国 《岩矿测试》2009,28(4):351-360

在简单介绍样品堆集法、pH法、扫集法、等速电泳法和色谱法等毛细管电泳在线富集技术的原理及对各个方法的优缺点评述的基础上,重点对2007—2008年毛细管电泳在线预富集技术研究取得的新进展进行综述。指出将两种或两种以上的富集方法相结合、对原有富集方法进行改进、开展基于新机理的富集方法是毛细管电泳在线富集技术研究的主要方向;建立新的高灵敏度富集技术更应将富集时间短、操作简单、重现性好和无需离线预处理即可完成对复杂基质中痕量及超痕量组分的富集方法作为研究重点。综述引用文献83篇。  相似文献   

Biochemical genetic study of sympatric Lake Constance whitefish (Coregonus lavaretus) populations: “Blaufelchen” and “Gangfisch”     

M. Luczynski  R. Rösch  J. A. Vuorinen  P. Brzuzan 《Aquatic Sciences - Research Across Boundaries》1995,57(2):136-143

Ripe females of whitefish (Coregonus lavaretus) of Lake Constance were caught at spawning time on their known spawning sites. Fish were examined for their size, gill-raker number, and for their egg dry weight. On the basis of their spawning sites as well as on correlation between body weight and egg weight, fish were allocated at one of the two sympatric populations: nearshore spawning Gangfisch or pelagic spawning Blaufelchen. The two fish samples were examined electrophoretically for the enzyme products of 37 genetic loci. Blaufelchen and Gangfisch were genetically identical (Nei's unbiased genetic distance D = 0.000) when all fish samples were included into the analysis. However, some genetic differentiation (D = 0.001) was evident after excluding the fish whose data points on body weight versus egg weight overlapped. Additional enzyme systems or some other genetic methodology should be used to provide more information on the genetic status of the two whitefish forms in Lake Constance.Contribution No. 8 in the Program of Joint Investigation of Holarctic Fishes among the Russia, Canada, Finland, and Poland  相似文献   

毛细管电泳及其在环境分析中的应用进展   总被引：3，自引：0，他引：3       下载免费PDF全文

袁倬斌  尚小玉张君 《岩矿测试》2003,22(2):144-150

对毛细管电泳技术的基本原理、特点以及分离模式进行了概述，对其在环境分析中的应用进展予以综述。  相似文献   

凡纳对虾个体发育早期的同工酶研究   总被引：4，自引：0，他引：4  

朱春华  李广丽  黄荣莲 《热带海洋学报》2004,23(1):51-57

采用聚丙烯酰胺梯度凝胶电泳技术，对凡纳对虾(Penaeus vannamei)个体发育早期6个时期的11种同工酶系统(EST、ALP、ACP、GDH、MDH、LDH、AAT、ME、LAP、ADH、AMY)进行研究。结果表明，凡纳对虾在幼体发育阶段已基本具备分解代谢蛋白质、脂类、碳水化合物的酶系。在早期发育过程中，EST同工酶系最为复杂，显示5-15条谱带，至少由6个位点编码；整个发育早期阶段GDH表达很弱，未见R-AMY及Q_AMY的表达；ALP、GDH、AMY及EST在无节幼体期表达很弱或未见表达；ACP、MDH、ME、ADH及AMY酶谱从糠虾幼体第Ⅲ期开始谱带数明显增加；除LAP、AAT酶谱简单且各期变化差异不大外，其它9种同工酶均随发育进程表现出明显差异。  相似文献   

大黄鱼肌浆蛋白和肝酯酶同工酶的电泳分析   总被引：2，自引：1，他引：2  

张海琪  李明云  钟爱华 《海洋学研究》2003,21(4):41-45

采用聚丙烯酰胺凝胶电泳(PAGE)技术,对大黄鱼肌浆蛋白(Pm)和肝酯酶(EST)同工酶进行研究。结果表明,大黄鱼的Pm电泳酶谱不存在个体间差异,而肝EST酶谱复杂,Est-2和Est-3为多态位点。X2检验表明,这两个多态位点等位基因频率都符合Hardy-Weinberg平衡。  相似文献