丁二酸柱撑水滑石的制备及表征     

陈国华  李长红  陈容  李苓 《中国海洋大学学报(自然科学版)》2007,37(6):983-986

以镍铝水滑石为前体（主体），以蒸馏水为分散介质，用离子交换法组装丁二酸根（客体）插层水滑石，同时用一步合成法合成丁二酸根插层水滑石，并用XRD，IR，TG-DTA和元素分析等手段对样品进行了表征。结果表明，离子交换法制得的产品中丁二酸根阴离子取代了镍铝水滑石前体层间的碳酸根离子，组装得到晶体结构良好的丁二酸根插层水滑石，层间距为1．23nm。  相似文献   

开链冠醚2，6-二(8''''-喹啉氧甲基)-4-甲基-苯甲醚及其Ag( Ⅰ )、Cu( Ⅱ )、Ni( Ⅱ )配合物的合成与性质研究     

张前前  季振平  萧文锦  吴成泰 《中国海洋大学学报(自然科学版)》2001,31(3):420-424

设计合成一种尚未见文献报道的酚醚型双臂开链冠醚化合物L(L=2,6-二(8’-喹啉氧甲基)-4-甲基-苯甲醚)，经元素分析、IR、1HNMR、MS等方法确定了其组成和结构。其Ag(Ⅰ)、Cu(Ⅱ)、Ni(Ⅱ)固体配合物的元素分析结果表明金属离子不同，则其配合物的配位比(M∶L)不同，Ag(Ⅰ)配合物为1∶1，Cu(Ⅱ)、Ni(Ⅱ)配合物为2∶3。并运用热重-差热分析对其热稳定性进行初步研究。  相似文献   

单细胞绿藻的77K荧光特异性研究   总被引：1，自引：0，他引：1  

刘健晖  李爱芬  刘太胜  孙岁寒  段舜山 《海洋科学》2007,31(7):68-72

实验测定了10种单细胞绿藻包括5种淡水藻:雪衣藻(Chlamydomonas nivalis UTEX2765)、椭圆小球藻(Chlorella ellipsoidea CH)、绿球藻(Chloroccum tatrense UTEX2227)、斜生栅藻(Scenedesmus obliquus)、雨生红球藻(Haematococcus sp.CSIRO:CS-321)和5种海水藻:杜氏盐藻(Dunaliella salina)、四爿藻(Tetraselmis sp.)、蒜头藻(Monodus subterraneus)、小新月藻(Closterium venus)、亚心形扁藻(Platymonas subcordiformis)的77k荧光光谱,并与大型海洋绿藻以及高等植物菠菜进行了比较。结果表明,10种单细胞绿藻都缺少作为高等植物PSⅠ主要表征的730nm长波荧光峰。按荧光主峰的波长,可以分为2种类型:雪衣藻、绿球藻、杜氏盐藻、四爿藻、蒜头藻、小新月藻、亚心形扁藻的荧光主峰位于684nm,椭圆小球藻、斜生栅藻、雨生红球藻的荧光主峰位于714nm。这个结果与海洋管藻目绿藻和某些大型海洋绿藻的荧光特性一致。另外,绿藻PSⅠ缺少730nm长波荧光峰具有普遍性,预示这些藻类在PSⅠ结构以及能量传递等方面与高等植物有很大的差异。  相似文献   

应用硫酸苯酚法建立一种甘露糖醛酸C-5差向异构酶活性检测方法的研究          下载免费PDF全文

林育姿  宫倩红  张真庆  韩?? 峰  于文功 《海洋科学》2007,31(2):48-51

建立了一种简便快捷的甘露糖醛酸C-5差向异构酶活性的测定方法。研究发现相同分子质量的聚甘露糖醛酸和聚古罗糖醛酸与苯酚硫酸试剂反应后显色程度不同,以此可以用来测定β-D-甘露糖醛酸/α-L-古罗糖醛酸(M/G)值的变化,从而计算甘露糖醛酸C-5差向异构酶的活性。利用本方法测定了已知结构的褐藻胶片断的M/G比,其结果与传统的1H-NMR测定值基本一致。  相似文献   

Quantitative mapping of active mud volcanism at the western Mediterranean Ridge-backstop contact     

Alain Rabaute  Nicolas Chamot-Rooke 《Marine Geophysical Researches》2007,28(3):271-295

Based on a new quantitative analysis of sidescan sonar data combined with coring, we propose a revised model for the origin for Mediterranean Ridge mud volcanism. Image analysis techniques are used to produce a synthetic and objective map of recent mud flows covering a 640 × 700 km² area, which represents more than half of the entire Mediterranean Ridge mud belt. We identify 215 mud flows, extruded during the last 37,000–60,000 years. This time period corresponds to the limit of penetration of the sonar, that we evaluate through geoacoustic modeling of the backscattered signal returned by the mud breccia-hemipelagites contact, and calibrate by coring. We show that during this period, at least 96% of the mud volume has been extruded at the Mediterranean Ridge-Hellenic backstop contact, the remaining being scattered over the prism. We suggest that the source is a Messinian (5–6 Ma) mud reservoir that remained close to the backstop contact, at variance with the classical transport-through-the-wedge model. A revised mud budget indicates that steady-state input is not needed. We propose that the source layer was deposited in deep and narrow pre-Messinian basins, sealed by Messinian evaporites, and finally inverted in post-Messinian times. Onset of motion of the Anatolia-Aegea microplate in the Pliocene resulted in change from slow to fast convergence, triggering shear partitioning at the edges of the backstop and basin inversion. Mud volcanism initiation is probably coeval with the latest events of this kinematic re-organization, i.e. opening of the Corinth Gulf and activation of the Kephalonia fault around 1–2 Ma.  相似文献   

Magnetic characterization of a retroarc extensional basin: The Loncopué Trough     

《Journal of South American Earth Sciences》2019

The Loncopué Trough is a Pliocene to Quaternary extensional basin developed over the hinterland area of the Southern Central Andes. This basin is bounded by two neotectonic extensional fault systems delimiting a narrow topographic low. Previous studies have mostly focused on structural and geochemical aspects of this feature. However, geophysical surveys, aimed to unravel deep structure beneath a thick-younger than 5 My volcanic coverage, are scarce and based their interpretations on low resolution data sets. In this study, we collected new aeromagnetic data with the objective of characterizing the magnetic properties of the crust in the Loncopué Through and nearby zones. Additionally, we analyze the spatial relation between geological structures, volcanic fields and magnetic data. In order to highlight the boundaries of the magnetic sources and calculate the basement depth, we applied derivative techniques and the source parameter imaging. Also, we estimated an effective susceptibility model using the Magnetization Vector Inversion method, which takes into account the combined effects of remanence and induced magnetization. To determine the thermal structure of the area, we calculated the Curie depth points through the spectral analysis technique. From the analysis of magnetic data we were able to characterize the main structures and lineaments associated with this retroarc extensional trough. Notably, only the Loncopué eastern fault system seems to be a crustal-scale tectonic feature, while E-W-, ENE- and ESE-trending lineaments are interpreted as relatively minor structures segmenting the basement. Finally, our susceptibility model, together with the analysis of the Curie point, revealed potential magmatic/hydrothermal reservoirs in the Copahue volcano, and the Codihue and Cajón de Almanza regions that could be connected at depth forming a regional magmatic body.  相似文献   

条斑紫菜锰超氧化物歧化酶原核表达及多克隆抗体制备   总被引：1，自引：0，他引：1  

邵林  茅云翔  阎斌伦  孔凡娜  王健 《中国海洋大学学报(自然科学版)》2010,40(4)

研究紫菜抗逆的分子机制,将本实验室克隆的条斑紫菜(Porphyra yezoensisUeda)锰超氧化物歧化酶(manganese superoxide dismutase,Mn SOD)基因全长cDNA克隆到质粒pET-30c(+)中,构建原核表达载体pET-S,转化大肠杆菌BL21(DE3)表达Mn SOD,表达产物的分子量约为30 KDa。利用不同浓度的Mn2+诱导重组Mn SOD的表达,可以检测到比活力随Mn2+浓度增高而上升的趋势。用重组Mn SOD免疫新西兰大白兔,制备了多克隆抗体,多克隆抗体的效价为1∶8 000。免疫印迹实验(Western Blot)表明该多克隆抗体具有很好的特异性。  相似文献   

海洋芽孢杆菌Bacillus sp. CAMT22370所产葡萄糖氧化酶的分离纯化与表征     

聂绮倩  莫日坚  徐德峰  孙力军  王雅玲  叶日英 《海洋科学》2018,42(5):68-76

前期筛选获得一株海洋源Bacillussp.CAMT22370高产葡萄糖氧化酶菌株,本研究进一步通过离子交换及分子筛层析使Bacillus sp. CAMT22370葡萄糖氧化酶纯化了2.12倍,酶活得率32.37%,比活力达到8.47 U/mg,分子质量约为50 kDa。酶学特性研究表明,此酶在低温下有较好的催化活性,最适温度为30℃,超过30℃时酶活迅速下降, 45℃保温2 h活力降至75%左右;最适pH为6.0,在5.0以下和7.0以上时酶活迅速降低, 9.0时损失35%; Ca~(2+)和Mn~(2+)有明显激活作用, Na~+和Zn~(2+)有轻微激活作用, K~+和Mg~(2+)对酶活无显著影响,而Fe~(2+)、Fe~(3+)和Cu~(2+)对酶活有明显抑制作用,酶动力学参数Vmax及Km值分别为26.85 (μmol/(L·min))和109.26 (μmol/(L·min)),以上酶学特性与现有酶制剂存在明显不同,研究结果为该葡萄糖氧化酶的开发利用奠定了初步基础。  相似文献   

海洋细菌QY202产κ-卡拉胶酶的分离纯化和性质研究   总被引：1，自引：0，他引：1  

段高飞  苏贝  韩峰  于文功 《中国海洋大学学报(自然科学版)》2010,40(3)

为获得高效降解卡拉胶菌株,从青岛太平角海域采集的角叉菜表面分离到1株高产κ-卡拉胶酶的海洋交替假单胞菌(Pseudoalteromonas sp.)QY202,经硫酸铵沉淀、脱盐、DEAE阴离子交换层析等步骤从该菌株发酵液上清中分离纯化得到1种专一性降解κ-卡拉胶的κ-卡拉胶酶,并研究了该酶的基本酶学性质.结果表明该酶被纯化了23.1倍,回收率为43.9%,分子量大小为33.2 kDa.酶的最适反应温度为40 ℃,最适反应pH为8.0,在0～40 ℃,pH=7.0～8.0之间酶活力较稳定.酶对底物κ-卡拉胶的米氏常数Km值为1.6 mg/mL.Na~+、K~+对酶活有促进作用,而Hg~(2+)、Cu~(2+)强烈抑制酶的活性.酶解κ-卡拉胶的主产物为硫酸新κ-卡拉二糖和硫酸新κ-卡拉四糖.  相似文献   

Sequences Characterization of Microsatellite DNA Sequences in Pacific Abalone(Haliotis discus hannai)   总被引：1，自引：0，他引：1  

Kijima Akihiro 《中国海洋大学学报(英文版)》2007,(1)

1 Introduction Microsatellites or simple sequence repeats (SSRs) are tandemly repeated motifs of one to six bases found in all prokaryotic and eukaryotic genomes analysed to date (Zane et al., 2002). Due to their hyper-variable and co-dominant nature, relatively high abundance and random distribution in the genome, microsatellites are among the most efficient class of molecular markers. Such repeats display high polymorphism because of variation in repeat length and can be rapidly analysed t…  相似文献