原生动物单细胞PCR应用初探——以天蓝喇叭虫为例     

刘志新  余育和 《湖泊科学》2008,20(5):669-674

以天蓝喇叭虫(Stentor coeruleus)为研究对象,探索了单细胞单基因PCR扩增及单细胞全基因组PCR扩增技术在原生动物中的应用.经过不断探索和优化条件后,试验取得了理想的结果.在40例单细胞单基因(SSU rDNA基因全序列)PCR的一次性扩增中,新鲜细胞和经过中性红染色的细胞都获得了100%的成功率,室温下酒精(95%)保存一周的细胞获得了82.5%的成功率.在单细胞全基因组PCR扩增中,采用高效高保真的phi29 DNA聚合酶结合随机引物(Random Primer)进行扩增,获得了丰富且质量较高的PCR产物.以全基因组扩增产物(稀释10倍)对四个常用的基因位点(TEF1、SSU rRNA、18S-ITS1-5.8S、a-tubulin)进行扩增,均成功获得相应的基因片断.  相似文献   

南极磷虾(Euphausia superba)线粒体基因组特征及其分子标记应用     

申欣  孙松  王海青  王敏晓  任建峰  张光涛  刘斌 《海洋与湖沼》2008,39(5):446-454

为揭示南极磷虾（Eupahusia superba）（甲壳动物：软甲纲：磷虾目）线粒体基因组特征,及通过比较不同海域南极磷虾的线粒体基因组探讨潜在的分子标记,采用Long PCR扩增技术获得采自普里兹湾（PrydzBay）南极磷虾线粒体基因组DNA,综合Primers-walking和Shotgun方法进行测序：通过生物信息学软件（DOGMA、tRNAscan-SE 1．21和DnaSP4．10．7等）进行基因预测及序列分析。南极磷虾线粒体基因组全长15498bp以上（部分非编码区未测定）,包含13个蛋白编码基因、2个核糖体RNA基因和23个转运RNA基因。与后生动物线粒体基因组标准的基因组成相比,存在1个trnN基因的重复。南极磷虾线粒体基因组的基因排列与泛甲壳动物线粒体基因组的原始排列相比,出现4个转运RNA基因的易位：trnL1、trnL2、trnW和trnl以及1个trnN的重复。比较采自普里兹湾（Prydz Bay）和威德尔海（Weddell Sea）南极磷虾的线粒体基因组,作者发现在南极磷虾线粒体基因组的主编码基因中,变异最大的是nad2基因,差异位点高达61个,其次是nad5基因,差异位点有12个：而coxl基因的变异位点仅有3个。Nad2基因和nad5基因可作为coxl基因辅助的分子标记,用于分析南极磷虾不同地理种群之间的遗传多样性,为合理利用南极磷虾生物资源提供保障。  相似文献   

基于倾斜摄影三维模型的大比例尺地形图测图软件比较与分析     

马旭文  徐柳华 《测绘与空间地理信息》2020,(2):57-59,62

针对当前市场上基于倾斜摄影三维模型采编大比例尺地形图软件种类繁多的现状,本文以目前市场上主流的3款采编软件--EPS,DP-Modeler和Idata3D为例,通过选取一个测试区域,对3款软件加载倾斜摄影三维模型、采集建筑物模型、采编DLG等技术性能进行深入对比分析。本文提出科学客观的分析以及评价意见,从而为基于倾斜摄影三维模型的大比例尺地形图测图应用提供翔实的技术指导,进一步推广应用新型地形图采编技术方法。  相似文献   

Bioprospection of marine actinomycetes: recent advances, challenges and future perspectives     

Sharma Swati  Fulke Abhay B.  Chaubey Asha 《海洋学报(英文版)》2019,38(6):1-2

In exploring new sources for economically important products, marine environment draws particular attention because of its remarkable diversity and extreme conditions; it is known to produce metabolic products of great value. It represents untapped source for the discovery of novel secondary metabolites with varying potential such as antibiotic, anti-tumor, antifouling and cytotoxic properties. Marine actinomycetes distributed throughout the marine environment from shallow to deep sea sediments have proved to be a finest source for this discovery. Secondary metabolites derived from marine actinomycetes have proved their worth in industries based on the research on their properties and wide range applications. Spotlight of the review is range of marine based actinomycetes products and significant research in this field. This shows the capability of marine actinomycetes as bioactive metabolite producers. Additionally, the present review addresses some effective and novel approaches of procuring marine microbial compounds utilizing the latest screening strategies of drug discovery from which traditional resources such as marine actinobacteria has decreased due to declining yields. The aim is in the context of promoting fruitful and profitable results in the near future. The recent surfacing of new technologies for bioprospection of marine actinomycetes are very promising, resulting in high quality value added products, and will be de? ning a new era for bioactive compounds with medical and biotechnological applications.  相似文献   

东方小藤壶Chthamalus challengeri（甲壳动物亚门：无柄目）线粒体基因组：小藤壶科基因排列比较及藤壶亚目系统发育分析     

陈盼盼  宋隽  申欣  蔡月凤  朱嘉濠  李咏琪  田美 《海洋学报(英文版)》2019,38(6):25-31

藤壶是潮间带生态、幼体发育以及生物防污损研究中重要的模式生物。目前,线粒体基因组学的发展有助于从线粒体基因组水平上更好地理解系统发育关系。本研究获得东方小藤壶Chthamalus challengeri完整线粒体基因组,大小为15358bp的环状分子。与藤壶亚目其它物种相比,东方小藤壶非编码区的长度较长,而基因区的长度则相近。东方小藤壶线粒体基因组A+T含量为70.5%。现有藤壶物种的线粒体基因组中存在起始和终止密码子的变化。同属的东方小藤壶和触肢小藤壶C. antennus具有共同的基因排列。然而,小藤壶科中不同属之间却具有不同的基因排列,包括两个tRNA基因出现易位,一个基因块出现倒置。值得注意的是,与以往藤壶亚目物种不同,小藤壶属两个物种的srRNA和lrRNA基因都在重链上编码。小藤壶科中进化树的拓扑结构与基因排列证据相互支持,系统发育分析表明小藤壶科是单系群,而藤壶科和古藤壶科则是多系群。  相似文献   

The complete mitochondrial genome of the hybrid grouper Epinephelus moara (♀)×Epinephelus tukula (♂), and phylogenetic analysis in subfamily Epinephelinae     

Zhentong Li  Yongsheng Tian  Meiling Cheng  Linna Wang  Jingjing Zhang  Yuping Wu  Zunfang Pang  Wenhui Ma  Jieming Zhai 《海洋学报(英文版)》2020,39(12):65-75

The mitochondrial genome (mitogenome) of hybrid grouper Epinephelus moara (♀)×Epinephelus tukula (♂), a new hybrid progeny, can provide valuable information for analyzing phylogeny and molecular evolution. In this study, the mitogenome was analyzed using PCR amplification and sequenced, then the phylogenetic relationship of E. moara (♀)×E. tukula (♂) and 35 other species were constructed using Maximum Likelihood and Neighbor-Joining methods with the nucleotide sequences of 13 conserved protein-coding genes (PCGs). The complete mitogenome of E. moara (♀)×E. tukula (♂) was 16 695 bp in length, which contained 13 PCGs, 2 rRNA genes, 22 tRNA genes, a replication origin and a control region. The composition and order of these genes were consistent with most other vertebrates. Of the 13 PCGs, 12 PCGs were encoded on the heavy strand, and ND6 was encoded on the light strand. The mitogenome of the E. moara (♀)×E. tukula (♂) had a higher AT nucleotide content, a positive AT-skew and a negative GC-skew. All protein initiation codons were ATG, except for COX and ND4 (GTG), ATP6 (CTG), and ND3 (ATA). ND2, COXII, ND3, ND4 and Cytb had T as the terminating codon, COXIII’s termination codon was TA, and the remaining PCGs of that were TAA. All tRNA genes, except for the lacking DHU-arm of tRNASer (AGN), were predicted to form a typical cloverleaf secondary structure. In addition, sequence similarity analysis (99% identity) and phylogenetic analysis (100% bootstrap value) indicated that the mitochondrial genome was maternally inherited. This study provides mitogenome data for studying genetic, phylogenetic relationships and breeding of grouper.  相似文献   

图形存储结构对地图制图软件中图形编辑功能的影响     

杨勇  叶民盛  刘敏 《测绘工程》2006,15(4):41-44,57

地图制图软件中,图形编辑功能与其撤消/重做(Undo/Redo)功能是必不可少的,一个好的图形存储结构能方便图形编辑操作及其Undo/Redo功能的实现。文中介绍了基于Oracle数据库的地图制图软件中图形数据的储存结构,其中包括数据库中的图形储存;图形数据从数据库中读入内存后的存储结构和图形编辑数据存储结构。在储存结构的基础上提出图形编辑及其Undo/Redo执行方法,并给出具体的实现代码,实验证明该方法提高了运行效率,减少了内存消耗。  相似文献   

地理信息数据库缩编方法的探讨     

曲平  王铁军  梁文光 《测绘与空间地理信息》2012,(6):75-76,79

介绍了自动缩编、直接缩编、标编缩编等几种数据库缩编方法,讨论了缩编流程及分要素缩编的实现,并将几种缩编方法进行了对比分析。  相似文献   

《黑龙江省地图集》的设计研究     

赵友兴 《测绘软科学研究》2011,(6):57-59

介绍《黑龙江省地图集》的设计、编辑特色,图集的内容结构,并重点对市县图组的编排顺序、市县地理方位的表述进行研究。  相似文献   

浅议地理教材中地图的编制原则     

王建军 《测绘与空间地理信息》2011,34(1):264-265

地图在地理教学中有突出的地位,地图的配备是地理教材编写中非常重要的环节,配备合适的地图能增加地理教材的科学性和趣味性。主要分析了编制地理教材中地图的原则与要求。  相似文献