预处理方法对海洋微藻Isochrysis zhanjiangenisis脂肪酸组成及稳定碳同位素的影响     

姚敬元  刘瑀  李颖  王海霞 《海洋学报(英文版)》2016,35(6):1-6

This study aims to quantify the effects of different pretreatment methods on the stable carbon isotope values of fatty acids in marine microalgae(Isochrysis zhanjiangenisis).To identify the effects of sample preparation on theδ~(13)C value and the fatty acid composition,we examined eight types of pretreatment methods including:(a) drying the sample followed by direct methyl esterification using HCl-CH_3OH;(b) drying the sample followed by direct methyl esterification using H_2SO_4-CH_3OH;(c) drying the sample by ultrasonic extraction and methylesterification using HCl-CH_3OH;(d) drying the sample by ultrasonic extraction and methyl-esterification using H_2SO_4-CH_3OH;(e) fresh sample followed by direct methyl-esterification using HCl-CH_3OH;(f) fresh sample followed by direct methyl-esterification using H_2SO_4-CH_3OH;(g) fresh sample with ultrasonic extraction followed by methyl-esterification using HCl-CH_3OH,and(h) fresh sample with ultrasonic extraction followed by methylesterification using H_2SO_4-CH_3 OH.The results show that the δ~(13)C values from Groups a–e,g and h fluctuated within 0.3‰,and the δ~(13)C values of Group f were approximately 0.7‰ lower than the other seven groups.Therefore,the different sample pretreatment methods used towards the extraction of fatty acids from marine microalgae may result in different results regarding the stable carbon isotope ratios,and if necessary a correction should be applied.  相似文献   

A comparative study of microalgal production in a marine versus a river-dominated temporarily open/closed estuary, South Africa     

Akash Anandraj  Renzo Perissinotto  Christian Nozais   《Estuarine, Coastal and Shelf Science》2007,73(3-4):768-780

Temporarily open/closed estuaries (TOCEs) account for almost 71% of the total number of South African estuarine systems. To date the dynamics of microalgal production and biomass in TOCEs is poorly understood. A survey was therefore conducted in two TOCEs along the KwaZulu-Natal coast: the river-dominated Mdloti and the marine-dominated Mpenjati. The aims of this investigation were: (1) to compare the variability of benthic and pelagic microalgal production and biomass in these two contrasting systems; (2) to determine the key environmental parameters influencing primary production in these TOCEs. Results show a similar pattern of primary production and biomass in both estuaries. High benthic microalgal biomass with low primary production and low phytoplankton biomass with high rates of pelagic primary production were observed in both estuaries. Possible explanations for this include: (a) optimum light conditions in the water column, compared to the sediment surface; (b) high impact of grazing by zooplankton in the water column; and (c) settling of phytoplankton. Significant correlations were observed between primary production and environmental parameters (K_d, PAR, temperature, DIN and DIP), during the open and closed phases of both estuaries. A BIOENV (PRIMER) analysis showed that patterns of primary production in both estuaries were influenced primarily by interactions of multiple independent parameters. Comparisons between measured hourly pelagic primary production rates at the Mpenjati and Mdloti and the composite parameter (P_c) of Cole and Cloern (1987) revealed highly significant relationships (r² = 0.8; p < 0.001) between these differently derived variables.  相似文献   

Standing crop and primary production of benthic microalgae on tidal flats in the Sanggou and Jiaozhou Bays, China     

Ning Xiuren  Cai Yuming  Liu Zilin  Hu Xigang  Liu Chenggang 《海洋学报(英文版)》2003,22(1):75-87

The standing crop and primary production of benthic microalgae on tidal flats have seasonally been observed in the Sanggou and Jiaozhou Bays of the northern China coast during 1999 - 2000. The results show that the annual primary productions of benthic microalgae on tidal flats in the two bays are 2 532 and 7 542 tons carbon, which would be able to support 3.1 x 103 and 9.2 x 103 tons shellfish meat, respectively. The nature of sediment is essential for governing the biomass and primary production of benthic microalgae on tidal flats. The biomass and primary production of benthic microalgae on tidal flats with muddy sediments are higher than those with sandy sediments, which resulted from higher nutrient concentration and lower grazing pressure. That is the reason why in comparison with Xiangshan Bay the standing crop and production of benthic microalgae on tidal flats of the Sanggou and Jiaozhou Bays are low. Light was a key factor controlling vertical distribution of biomass and production of benthic  相似文献   

融合微藻Tetraselmis sp.-1兼养培养条件下的动力学研究     

沈继红  林学政  李光友  刘发义 《海洋科学进展》2003,21(2):226-231

利用统计分析系统(Statistic Analysis System,SAS),对融合微藻Tetraselmis sp.-1兼养培养进行研究,建立了兼养条件下的细胞生长、底物消耗和产物生成3种动力学模型.经过数据验证,3种模型均能较好的反映微藻培养过程.  相似文献   

海洋微藻的体外抗肿瘤活性初筛   总被引：1，自引：0，他引：1       下载免费PDF全文

卢圳域  朱伟明  顾谦群  刘培培  刘茂新  郭健  管华诗 《海洋与湖沼》2007,38(5):464-467

采用小鼠乳腺癌细胞株tsFT210的细胞周期抑制、细胞凋亡诱导、细胞坏死以及对卤虫的毒性为活性指标,对48种海洋微藻的甲醇提取物的抗肿瘤活性进行了筛选。结果表明,以卤虫毒性为活性指标时,9种海洋微藻显示了不同程度的抗肿瘤活性,以tsFT210为筛选模型时,也有9种具有抗肿瘤活性,其中3种为明显的细胞凋亡诱导活性、2种为细胞坏死性活性、1种为G2/M期抑制活性,因此有必要进行开拓海洋微藻药用新资源的研究。  相似文献   

UV-B辐射对2种海洋微藻膜脂过氧化和脱酯化伤害     

王军  肖慧  冯蕾  蔡恒江  唐学玺 《中国海洋大学学报(自然科学版)》2006,36(5):763-766

研究海洋生态系统在调节全球变化中的作用,采用生态毒理学的方法研究了UV-B辐射增强对2种海洋微藻膜的损伤及其差异性。结果表明,随着UV-B辐射剂量的增加,叉鞭金藻(Dicrateriasp.)和三角褐指藻(Phaeodactylum tricornu-tum)的光合速率降低。同时,膜相对透性增大,细胞内H2O2含量上升,微粒体膜中磷脂减少,游离脂肪酸增加。表明UV-B辐射增强使2种微藻的膜受到了严重伤害,而且膜伤害的加剧与活性氧的积累和由此引发的膜脂脱酯化有关。2种微藻细胞匀浆中丙二醛(MDA)含量无显著变化,而微粒体膜的MDA含量随着UV-B辐射剂量的加大显著提高。这表明UV-B辐射增强对2种海洋微藻膜的损伤可能是由膜脂过氧化和脱酯化作用共同引起的。  相似文献   

几种微藻对珠母贝面盘幼虫生长和存活的影响   总被引：1，自引：0，他引：1  

周银环  黄海立  邓陈茂  符韶 《台湾海峡》2007,26(2):249-255

研究了不同浓度的湛江等鞭金藻（Isochrysis zhanjiangensis Hu ＆Liu）、亚心形扁藻[Platymonas subcordiformis（Wille）Hazen]、小球藻（Chlorella sp.）以及这3种微藻以一定的比例混合后（简称混合藻）对珠母贝面盘幼虫生长和存活的影响.结果表明：幼虫对3种微藻都能摄食,但4种饵料相比,以湛江等鞭金藻和混合藻的饵料效果为好,亚心形扁藻在面盘幼虫发育到93μm时可以投喂,小球藻饵料效果较差,不宜单独作为面盘幼虫的饵料;湛江等鞭金藻、亚心形扁藻和混合藻的浓度对面盘幼虫生长和存活都有显著影响,小球藻对其没有显著的影响.湛江等鞭金藻和混合藻适宜的投喂浓度分别为1 000-1 500个/cm^3和1 500-2 000个/cm^3,在适宜浓度下,湛江等鞭金藻组面盘幼虫最大生长速度、存活率和壳初率以及幼虫进入壳初的时间分别为5.663μm/d、69.34%、63.68%、6d,混合藻组为6.28μm/d、69.46%、54.49%、5-6 d.  相似文献   

基于UPLC-Q-Exactive MS构建麻痹性贝类毒素的特征指纹溯源技术          下载免费PDF全文

张帆  郑关超  王潇潇  翟毓秀  谭志军  吴海燕 《海洋与湖沼》2024,55(1):135-144

麻痹性贝类毒素污染是世界共同关注和重点管控的食品安全问题, 其溯源一直是产业监管的难点和技术重点。基于指纹溯源技术理论, 采用室内模拟3种产毒藻暴露紫贻贝后风险形成过程, 开发基于超高效液相色谱-四极杆-静电场轨道阱高分辨质谱(UPLC-Q-Exactive MS)鉴定麻痹性贝类毒素区域特征的指纹溯源技术。结果表明, 采用乙腈/甲醇/丙酮(体积比为1︰1︰1)混合溶液作为提取剂, C8色谱柱联合正离子模式, 质荷比(m/z) 100~600和Amide色谱柱联合正离子模式, m/z 600~1 500作为色谱质谱检测条件下, 提取的化合物数量较多且稳定性良好, 覆盖了40.4%指纹信息; 紫贻贝与产毒藻中的共检出11种毒素成分, 且具有高度相关性。N-磺酰胺甲酰基类毒素(如C类)和膝沟藻毒素(GTX类)在暴露期发生了显著的代谢转化; 将摄食3种产毒藻后紫贻贝的差异化合物与特征毒素组分进行主成分分析及正交偏最小二乘法判别分析, 共筛选出13种复合指纹物质。构建6种特征物质的Fisher判别模型, 交叉验证准确率为88.9%, 可实现产毒藻种溯源。该研究建立了基于超高效液相色谱-四极杆-静电场轨道阱高分辨质谱UPLC-Q-Exactive MS紫贻贝特征指纹溯源技术, 初步实现了从污染麻痹性贝类毒素的贝类到肇事藻种的逆向溯源,可应用于贻贝麻痹性贝类毒素风险溯源研究。  相似文献   

4种海洋微藻对久效磷的抗性与其抗氧化能力的相关性   总被引：16，自引：2，他引：14  

唐学玺  李永祺 《海洋与湖沼》2000,31(4):414-418

于１９９４年８月－１９９５年１２月,动用生态毒理学和生物化学实验方法,选用扁藻、叉鞭金藻、三角褐指藻及盐藻４种海洋微藻为材料进行海洋微藻对效磷的抗性与抗氧化能力相关性的实验。结果表明,久效磷在协迫过程中,微藻细胞内产生了过量的对细胞有伤害作用的活性氧,４种海洋微藻细胞内活性氧清除酶－超氧化物歧化酶的活性高低依次为盐〉三角褐指藻〉叉鞭金藻〉扁藻。比较毒性实验证明：４种海洋微藻对久效磷的基本身抗氧化能  相似文献   

微藻异养培养技术的研究进展   总被引：8，自引：0，他引：8  

张海滨  孙世春  麦康森  梁英 《海洋湖沼通报》2000,(3):51-59

微藻作为水产动物的活铒料,在水产养殖中一直有着广泛的应用.很多微藻营养价值高,且含有丰富的生物活性物质,既是海洋药物的潜在来源, 也是人类优质的保健食品(Vincent,1969,Schwarz et al,1995).  相似文献