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21.
22.
Koji Suzuki Akira Hinuma Hiroaki Saito Hiroshi Kiyosawa Hongbin Liu Toshiro Saino Atsushi Tsuda 《Progress in Oceanography》2005,64(2-4):167
To verify the hypothesis that the growth of phytoplankton in the Western Subarctic Gyre (WSG), which is located in the northwest subarctic Pacific, is suppressed by low iron (Fe) availability, an in situ Fe fertilization experiment was carried out in the summer of 2001. Changes over time in the abundance and community structure of phytoplankton were examined inside and outside an Fe patch using phytoplankton pigment markers analyzed by high-performance liquid chromatography (HPLC) and flow cytometry (FCM). In addition, the abundance of heterotrophic bacteria was also investigated by FCM. The chlorophyll a concentration was initially ca. 0.9 μg l−1 in the surface mixed layer where diatoms and chlorophyll b-containing green algae (prasinophytes and chlorophytes) were predominant in the chlorophyll biomass. After the iron enrichment, the chlorophyll a concentration increased up to 9.1 μg l−1 in the upper 10 m inside the Fe patch on Day 13. At the same time, the concentration of fucoxanthin (a diatom marker) increased 45-fold in the Fe patch, and diatoms accounted for a maximum 69% of the chlorophyll biomass. This result was consistent with a microscopic observation showing that the diatom Chaetoceros debilis had bloomed inside the Fe patch. However, chlorophyllide a concentrations also increased in the Fe patch with time, and reached a maximum of 2.2 μg l−1 at 5 m depth on Day 13, suggesting that a marked abundance of senescent algal cells existed at the end of the experiment. The concentration of peridinin (a dinoflagellate marker) also reached a maximum 24-fold, and dinoflagellates had contributed significantly (>15%) to the chlorophyll biomass inside the Fe patch by the end of the experiment. Concentrations of 19′-hexanoyloxyfucoxanthin (a prymnesiophyte marker), 19′-butanoyloxyfucoxanthin (a pelagophyte marker), and alloxanthin (a cryptophyte marker) were only incremented a few-fold increment inside the Fe patch. On the contrary, chlorophyll b concentration reduced to almost half of the initial level in the upper 10 m water column inside the Fe patch at the end of the experiment. A decrease with time in the abundance of eukaryotic ultraphytoplankton (<ca. 5 μm in size), in which chlorophyll b-containing green algae were possibly included was also observed by FCM. Overall, our results indicate that Fe supply can dramatically alter the abundance and community structure of phytoplankton in the WSG. On the other hand, cell density of heterotrophic bacteria inside the Fe patch was maximum at only ca. 1.5-fold higher than that outside the Fe patch. This indicates that heterotrophic bacteria abundance was little respondent to the Fe enrichment. 相似文献
23.
南大洋深海嗜冷菌2-5-10-1及其低温脂肪酶的研究 总被引:14,自引:0,他引:14
从南大洋普里兹湾的水样中筛选到一株产低温脂肪酶的深海嗜冷菌2-5-10-1,对其生长及产酶情况和酶性质做了初步研究.该菌株的最适生长温度为5℃,此时分泌的胞外脂肪酶最多;添加Tween80,橄榄油可显著促进脂肪酶的产生.该脂肪酶的最适作用温度为35℃,在0~20℃均保持较高的酶活性,在0℃可保持37%的相对酶活性;酶的最适作用的pH值为7.5,在pH6~9的范围内均存在较高酶活性;对热较敏感,在60℃保温15min可丧失50%以上的酶活性.该脂肪酶的催化作用不需要金属离子的参与,Cu2+和Zn2+对酶活有着强烈的抑制作用. 相似文献
24.
25.
Nicolas Mazzella Josiane Molinet Agung Dhamar Syakti Jean-Claude Bertrand Pierre Doumenq 《Marine Chemistry》2007,103(3-4):304-317
This in situ study reports on the relevance of intact phospholipids as new biomarkers and highlights the limits of the classical fatty acid analysis after the hydrolysis of the polar lipid fraction. The analysis of polar lipid fractions revealed significant contributions of marine macrophytes with either non-phospholipid compounds (sulfoquinovosyl diacylglycerol) or with both non-bacterial phospholipid classes (phosphatidylcholine) and non-bacterial phospholipid molecular species (some molecular species of phosphatidylglycerol). The analysis of intact phospholipids showed also the predominance of gram-negative bacteria in sediments whereas the analysis of fatty acids alone cannot confirm such information. Lastly, as regard to the effects of petroleum hydrocarbons on the sedimentary bacterial communities, we observed a higher degree of saturation of the fatty acyl chains of the phospholipids extracted from sediment exposed to a massive and chronic crude oil contamination. Such a result was interpreted as an adaptive response resulting in higher membrane rigidity of the bacterial communities. 相似文献
26.
Mussel Periostracum from Deep-Sea Redox Communities as a Microbial Habitat: 3. Secondary Inhabitants
Abstract. Vacated microborings in periostracum of live mussels of the Florida Escarpment redox community (depth of 3266 m) become a habitat for a prolific secondary microbiota consisting of a variety of prokaryotic and a few eukaryotic organisms. Periostracum surface is also colonized by diverse microorganisms, dominated by presumed chemolithotrophic bacteria with stacks of intracellular lamellae. Unlike sheltered microflora within borings, the surface community is heavily grazed upon by numerous archaeogastropods and ciliates. 相似文献
27.
The atomic force microscopy (AFM) is a recently developed, bench-top instrument that can image the surface structures of biological
specimens at high resolution with simultaneous measurement of their size. This paper describes the application of AFM to marine
bacteria. Both natural and cultured bacteria were retained on a filter or placed on glass, washed, air-dried and observed
by AFM. The instrumental condition, the choice of suitable filter, effect of fixation and filtration, comparison with epifluorescent
microscopic (EFM) count, and the size and shape of bacterial cells were investigated. An Isopore filter was best for concentration
and subsequent observation because of its surface flatness. Cross section images showed that both rod and coccoid cells were
flattened, the former usually having a two-humped shape. Bacterial cells were differentiated from non-living particles based
on their cross section shape and size. Bacterial counts by AFM and EFM showed good agreement. Although size measurement is
easily done by the instrument, AFM tends to overestimate the size of microspheres. More work is thus needed on the size measurement
of living organisms. Because AFM easily provides images of natural bacterial cells at high magnification, it can be used as
a new tool to study the fine structures of marine bacteria.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
28.
秋季东、黄海异养细菌(Heterotrophic Bacteria)的分布特点 总被引:27,自引:1,他引:27
2000年10-11月,乘"北斗号"考察船进行秋季"东、黄海生态系统动力学与生物资源可持续利用"大面调查,研究秋季东、黄海异养细菌的分布.结果表明,异养细菌在黄海和东海的丰度分别在(2.37-13.33)×108 cell/L和(3.05-13.62)×108 cell/L之间.细菌丰度最高值出现在长江口附近,且断面E和断面F各站位的细菌丰度明显高于其他断面.异养细菌丰度大小以长江口为中心向外海依次递减.东、黄海水体异养细菌生物量分别在244.45-1812.90mgC/m2和100.60-940.87mgC/m2之间.东、黄海异养细菌丰度无显著差异,但是东海海域水体异养细菌生物量高于黄海海域.异养细菌空间分布与浮游植物叶绿素在东海有一定的相关性,黄海异养细菌与硝酸盐浓度的相关性极显著. 相似文献
29.
Biological Responses to Seasonally Varying Fluxes of Organic Matter to the Ocean Floor: A Review 总被引:8,自引:0,他引:8
Andrew J. Gooday 《Journal of Oceanography》2002,58(2):305-332
Deep-sea benthic ecosystems are sustained largely by organic matter settling from the euphotic zone. These fluxes usually
have a more or less well-defined seasonal component, often with two peaks, one in spring/early summer, the other later in
the year. Long time-series datasets suggest that inter-annual variability in the intensity, timing and composition of flux
maxima is normal. The settling material may form a deposit of “phytodetritus” on the deep-seafloor. These deposits, which
are most common in temperate and high latitude regions, particularly the North Atlantic, evoke a response by the benthic biota.
Much of our knowledge of these responses comes from a few time-series programmes, which suggest that the nature of the response
varies in different oceanographic settings. In particular, there are contrasts between seasonal processes in oligotrophic,
central oceanic areas and those along eutrophic continental margins. In the former, it is mainly “small organisms” (bacteria
and protozoans) that respond to pulsed inputs. Initial responses are biochemical (e.g. secretion of bacterial exoenzymes)
and any biomass increases are time lagged. Increased metabolic activity of small organisms probably leads to seasonal fluctuations
in sediment community oxygen consumption, reported mainly in the North Pacific. Metazoan meiofauna are generally less responsive
than protozoans (foraminifera), although seasonal increases in abundance and body size have been reported. Measurable population
responses by macrofauna and megafauna are less common and confined largely to continental margins. In addition, seasonally
synchronised reproduction and larval settlement occur in some larger animals, again mainly in continental margin settings.
Although seasonal benthic responses to pulsed food inputs are apparently widespread on the ocean floor, they are not ubiquitous.
Most deep-sea species are not seasonal breeders and there are probably large areas, particularly at abyssal depths, where
biological process rates are fairly uniform over time. As with other aspects of deep-sea ecology, temporal processes cannot
be encapsulated by a single paradigm. Further long time-series studies are needed to understand better the nature and extent
of seasonality in deep-sea benthic ecosystems.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
30.