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61.
产复合酶菌株Pseudomonas sp.NJ197产酶发酵条件的研究 总被引:2,自引:0,他引:2
从南极普利兹湾深海900m的沉积物中筛选到一株同时产多种低温复合酶的菌 株NJ197,作者对其进行碳源、氮源、无机盐、起始pH值、培养时间、接种量等发酵条 件的优化实验.实验结果证明,在以0.5%可溶性淀粉为碳源,以0.5%豆饼粉为氮 源,无机盐:0.424%NH4H2PO4、0.075%K2HPO4、0.02%MgSO4、0.5%CaCO3,起始 pH值8.5,接种36h种龄的种子10%,20℃、250r/min的条件下在旋转摇床中培养 72h,产复合酶菌株产脂肪酶和淀粉酶活性最高.最佳的生理条件下复合酶中的碱性 脂肪酶和淀粉酶的产量分别提高到22.4U/cm3和30.9U/cm3,该产复合酶菌株可以 作为诱变育种、基因工程菌改造的出发菌株,在洗涤和制革工业中有良好的应用前 景. 相似文献
62.
63.
闽江口水动力和污染扩散数值模拟 总被引:3,自引:0,他引:3
本文建立了闽江口二维水动力、污染扩散数值模型,模型采用ADI双向隐式求解。在水动力模拟中,对岸界的丁坝有用线性边界进行拟合,并考虑到河口区侧向摩擦作用;在污染扩散模拟中,用动态拟合的方法对浓度水边界进行了较为合理的改进。计算结果表明,本模型是成功的。 相似文献
64.
The paper describes experimental tests carried out on three ring-stiffened cones that were tested to destruction under external hydrostatic pressure. The cones were carefully machined from EN1A Steel. All three cones failed by plastic non-symmetric bifurcation buckling in a mode commonly known as general instability. In this mode the entire ring-shell combination buckles bodily.The paper also provides a design chart using the results obtained from these three vessels, together with the results of six other vessels obtained from other tests. The design chart allows the possibility of obtaining a plastic knock down factor, so that the theoretical buckling pressures, based on elastic theory, can be divided by the plastic knockdown factor, to give the predicted buckling pressure. This method can also be used for the design of full-scale vessels. 相似文献
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66.
研究协方差阵Σ的二次型容许估计问题。设 y1,y2 ,… ,yniid,n≥ 2 ,y1与 p维正态分布N (β,Σ )有相同的前四阶矩。其中β =(β1,β2 ,… ,βp)′∈ Rp与Σ =(σij) p× p >0均未知。记 y =△ (y1,y2 ,… ,yn)′。在二次损失 L (d ,Σ ) =tr(d -Σ) 2下给出Σ的二次型估计 a S2 + nby-y-′是容许估计的必要条件为 :(n - 1) a + b + 2 max(a,b)≤ 1。此必要条件比张立振等协方差阵的二次型容许估计中的必要条件有了明显的加强 相似文献
67.
Hitoshi Kawabata Hisashi Narita Koh Harada Shizuo Tsunogai Masashi Kusakabe 《Journal of Oceanography》2003,59(5):651-661
Thirteen vertical profiles of 226Ra and 222Rn in the near-surface water were obtained in the western North Pacific in winter, and the gas transfer velocities across
the air-sea interface were estimated. The transfer velocities found by applying a steady state model varied widely from 2.1
to 30.2 m day−1 with a mean of 9.4 m day−1. The mean value is almost 5 times higher than that in summer in other oceans, and the maximum value is a record high for
world oceans. This is partly due to the inadequacy of the steady state model, which overestimates when stronger winds blow
in more recent days than the 222Rn half-life of about 4 days. In fact, a strong low pressure zone passed through the station about 2 days earlier, which was
one of the low pressure zones that with a period of develop once a week or so in the northwestern North Pacific in winter.
Instead of steady-state removal, if half of the radon removal occurred sporadically every 7 days, and the last removal took
place two days before the observation, the transfer velocity would be 26 m day−1. Our mean transfer velocity, which is less than 20% different from the steady state value including both overestimated and
underestimated values, 9.4 ± 4.8 m day−1, seems to represent the mean state of this region in winter. This suggests that the gas exchange fluxes under extremely rough
conditions in the open ocean are larger than those estimated by using a transfer velocity equation with a linear or quadratic
relationship with wind speed.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
68.
Waterfront retaining walls supporting dry backfill are subjected to hydrostatic pressure on upstream face and earth pressure on the downstream face. Under seismic conditions, if such a wall retains a submerged backfill, additional hydrodynamic pressures are generated. This paper pertains to a study in which the effect of earthquakes along with the hydrodynamic pressure including inertial forces on such a retaining wall is observed. The hydrodynamic pressure is calculated using Westergaard's approach, while the earth pressure is calculated using Mononobe-Okabe's pseudo-static analysis. It is observed that when the horizontal seismic acceleration coefficient is increased from 0 to 0.2, there is a 57% decrease in the factor of safety of the retaining wall in sliding mode. For investigating the effect of different parameters, a parametric study is also done. It is observed that if φ is increased from 30° to 35°, there is an increase in the factor of safety in the sliding mode by 20.4%. Similar observations were made for other parameters as well. Comparison of results obtained from the present approach with [Ebeling, R.M., Morrison Jr, E.E., 1992. The seismic design of waterfront retaining structures. US Army Technical Report ITL-92-11. Washington DC] reveal that the factor of safety for static condition (kh=0), calculated by both the approaches, is 1.60 while for an earthquake with kh=0.2, they differ by 22.5% due to the consideration of wall inertia in the present study. 相似文献
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70.
本文根据分析结果阐述了两种不同样品处理方法所获得的硅藻丰度和微型硅藻的人主要种类和相对百分含量。研究表明洗涤富集法所获得的硅藻丰度存在较大误差;微型硅藻的含量普遍介于10%-20%之间,最高可达42.31%,最低为5.66%,并指出了在微型硅藻相对含量大于20%的样品中 ,其主要种类为具槽直链藻和条纹小环藻等沿岸底格格不入中,并成群体产出。 相似文献