南极低温降解菌Shewanella sp. NJ49羟化酶分离纯化及最适产酶条件研究     

刘芳明  王以斌  曲长凤  梁强  郑洲  缪锦来  肖天 《海洋科学》2016,40(5):1-8

羟化酶是细菌降解烷烃的关键酶,本文以南极低温降解菌Shewanella sp.NJ49为研究对象,通过硫酸铵沉淀、超滤浓缩及葡聚糖凝胶Sephadex G-75柱层析方法,分离纯化NJ49的羟化酶,获得了单一羟化酶组分,研究了温度、p H、表面活性剂和盐度等环境因素对NJ49羟化酶酶活活性影响。结果表明,羟化酶组分由α、β、γ三个亚基组成,分子质量分别为56,45和36 k D。NJ49最适产酶条件为:温度15℃、p H7.2、盐度55;不同类型表面活性剂对酶的活性影响效应不一,两性离子表面活性剂、阳离子表面活性剂、非离子表面活性剂对酶活没有作用,阴离子表面活性剂+非离子表面活性剂混和剂和阳离子表面活性剂+非离子表面活性剂混和剂能提高羟化酶的活性。  相似文献   

反渗透海水淡化技术现状和展望     

高从堦  周勇  刘立芬 《海洋技术学报》2016,(1):1-14

反渗透膜技术于20世纪60年代取得突破性进展,促使反渗透海水淡化在近50年间高速发展,淡化产能自20世纪90年代起激增.海水反渗透(SWRO)淡化已成为目前投资最省、成本最低的利用海水制备饮用水的过程.文中主要对海水反渗透淡化的发展状况进行了介绍,如膜和组件的改进,关键设备高压泵和能量回收装置效率的提高,多种工艺过程的不断发展,包括预处理和后处理的新工艺,以及对环境的影响和相应对策等.反渗透技术的发展也推动了其他膜分离技术的进步,并扩展其应用领域.预计在不久的将来,膜技术在海水淡化和水再利用、扩大和保护水资源、循环经济、清洁生产、改造传统产业、节能减排及提高人民生活水平等方面发挥的作用会越来越显著.  相似文献   

Cutting of Phragmites australis as a lake restoration technique: Productivity calculation and nutrient removal in Wuliangsuhai Lake, northern China     

Jan Felix K&#;bbing  Niels Thevs  Stefan Zerbe 《寒旱区科学》2016,8(5):400-410

Reed is one of the most frequent and dominant species in wetlands all over the world, with common reed (Phragmites australis (Cav.) Trin. ex Steud.) as the most widely distributed species. In many wetlands, P. australis plays a highly ambivalent role. On the one hand, in many wetlands it purifies wastewater, provides habitat for numerous species, and is a potentially valuable raw material, while on the other hand it is an invasive species which expands aggressively, prevents fishing, blocks ditches and waterways, and builds monospecies stands. This paper uses the eutrophic reed-swamp of Wuliangsuhai Lake in Inner Mongolia, northern China, as a case to present the multiple benefits of regular reed cutting. The reed area and aboveground biomass production are calculated based on field data. Combined with data about water and reed nutrient content, the impact of reed cutting on the lake nutrient budget (N and P) is investigated. Currently, at this lake around 100,000 tons of reed are harvested in winter annually, removing 16% and 8% of the total nitrogen and phosphorus influx, respectively. Harvesting all available winter reed could increase the nutrient removal rates to 48% and 24%, respectively. We also consider the effects of summer harvesting, in which reed biomass removal could overcompensate for the nutrient influx but could potentially reduce reed regrowth.  相似文献   

煤田绳索取心钻探应建立新的行业钻探体系          下载免费PDF全文

黄才启  熊青山  黄全宜 《探矿工程》2021,48(9):71-80

煤炭仍然是世界主要能源资料,煤田地质勘探必将向深部发展,绳索取心钻探技术是深部钻探优质高效的首选方法。目前煤田绳索取心钻探技术未能全面采用,存在若干不适应煤田地层的技术问题,但都能采取措施解决。煤田地质钻探钻遇地层普遍存在水敏现象,导致泥浆含砂、含泥量过高使性能变坏。煤田绳索取心钻探还存在与“水敏”、“砂害”伴随着的泵压高、泥浆流速快、冲刷严重、冲洗液动力学效应负作用大等问题,采用非煤地质勘探标准钻具（柱）,强度低,能力弱,难以抵抗负面作用。为此,不仅需要优选适应地层抑制其水敏性的泥浆配比,更重要的是解决除砂除泥问题。同时要打破旧有思维,建立新体系,扩大钻孔直径,增大配套间隙,提升装备动力,用大功率去克服问题。  相似文献   

中国非常规水资源开发利用进展          下载免费PDF全文

马涛  刘九夫  彭安帮  郑锦涛  王文种  郑皓  邓晰元 《水科学进展》2021,31(6):960-969

非常规水资源开发利用已成为世界各国缓解水资源危机的重要举措之一。为深化对中国非常规水资源开发利用水平和现状的认知,更好地支撑非常规水资源开发利用管理和实践,在对不同类型非常规水资源开发利用实地调研的基础上,重点阐述非常规水资源的术语和内涵,总结非常规水资源的现状开发利用量和开发利用模式,分析非常规水资源开发利用中存在的主要问题,并针对性地提出对策建议。近年来中国加大了对非常规水资源的开发利用力度,非常规水资源开发利用量逐年上升,截至2018年底已达86.4亿m³,但在全国供水总量中的占比尚不足1.5%,开发利用中还存在体制机制不健全、规划设计不到位、工程技术有短板等问题,制约着非常规水资源开发利用的有序发展。非常规水资源开发利用涉及面广,需要社会各界的共同努力,才能迎来非常规水资源开发利用的新局面。  相似文献   

Anticipating the effects of groundwater withdrawal on seawater intrusion and soil settlement in urban coastal areas     

Josep Mas‐Pla  Agustí Rodríguez‐Florit  Manel Zamorano  Carles Roqué  Anna Menció  David Brusi 《水文研究》2013,27(16):2352-2366

Intensive pumping in urban coastal areas is a common threat to water resource quality due to seawater intrusion. In those areas where subsurface water resources are not usually used for human consumption or irrigation, intensive pumping is associated with other activities like the lowering of the water table necessary to support underground structures and building foundations. This activity also increases the likelihood of soil settlement that affects building stability and the corrosion of concrete structures due to groundwater salinity. Under these circumstances, the awareness of a certain municipality (Calonge, NE Spain) of the potential effects of groundwater withdrawal upon foundations has led to an integrated approach to anticipate seawater intrusion related to urban development. Geological mapping and correlation of borehole logs, electrical resistivity tomography, and hydrochemical data provide comprehensive knowledge of the geology and hydrogeology of the area and act as screening tools necessary to discern the influence of hydrological processes in coastal areas. Developing Strack's analytical solution, new comprehensive, dimensionless expressions are herein derived to determine the critical pumping rate necessary to prevent seawater intrusion, as well as to reproduce the evolution of the wedge toe and the water table stagnation point under different withdrawal rates. Furthermore, the Dupuit–Forchheimer well discharge formula allows the estimation of the effects of the water table lowering due to such critical pumping in the surrounding building foundations. Field data from the Calonge coastal plain illustrate this approach and provide assessment criteria for future urban development and planning. Copyright © 2012 John Wiley & Sons, Ltd.  相似文献   

离心式泥浆机械净化设备的研制与应用     

刘阳 《新疆地质》1989,(4)

本文介绍了两种离心式泥浆净化设备——NL-227型离心除泥器和WL-230型连续除泥离心机的结构、特点和应用效果,讨论了几种净化设备的分离物的粒度分布.  相似文献   

城市污水处理厂污泥利用现状及潜在环境问题   总被引：9，自引：0，他引：9  

陈军 《矿产与地质》2005,19(6):732-734

根据国家环保总局要求,在2010年前全国主要城市的污水处理率要达到70%,这将使我国的水环境得到明显改善,另一方面也带来了污泥的处置和利用问题.污泥是一种优质的有机肥料,有选择地控制使用,既可满足作物生长对养分的需要,又可培育土壤.符合污泥固废“减量化”和“资源化”的处置要求.  相似文献   

泥炭样品的AMS ¹⁴ C年龄测定: 全样、植物残体和孢粉浓缩物^*     

李宜垠  魏芳  周力平 《第四纪研究》2007,27(4):499-506

沉积物¹⁴ C的年龄测定一直是第四纪年代学研究的热点。文章对中国干旱、半干旱地区的内蒙古库伦泥炭剖面的泥炭全样、植物残体和孢粉浓缩物进行了AMS ¹⁴ C测年比较研究。从泥炭沉积物中提取用于AMS ¹⁴ C测年的孢粉浓缩物的关键步骤包括:过筛(125μm,63μm和10μm),重液浮选(比重1.9)及在180倍的体视显微镜下进行手工挑选。测年结果表明:孢粉浓缩物的测年值比同一层位的泥炭沉积物全样或植物残体的测年值老225~340年; 植物残体与孢粉浓缩物的测年结果较为接近。但是,距地表24~27cm处孢粉浓缩物给出了610~780A.D.的年龄值,远老于其下样点的年龄,而泥炭全样为现代样品,二者相差1255年,这可能与该样品孢粉纯度较低、掺杂有一些老碳物质燃烧后形成的微粒杂质有关。因此,提高孢粉纯度对孢粉浓缩物¹⁴ C测年至关重要。  相似文献   

Ground water hydrochemical characteristics:seawater intruded area in eastern and southern coast of Laizhou Bay     

ZHANG Zu-lu  JIANG Lu-guang  YANG Li-yuan  QI Yong-hua 《地理学报》2001,11(3):350-355

Eastern and southern coastal zones of Laizhou Bay are the most representative seawater intruded areas in the world, with two intrusion sources of contemporary seawater and paleobrine. In order to reveal the complicated hydrochemical changing process and the mechanism of fresh groundwater being polluted by saltwater, we conducted long-term observation and hydrochemical analysis at four observing sections of typical salt-fresh water transitional zone. The study indicates that seawater and brine intrusion processes have different hydrochemical features, and that ion exchange and adsorption actions between water and aquifer produce great influence on the intrusion.  相似文献