吸附-交联法固定胰蛋白酶及在澄清啤酒中的应用     

温燕梅  邱彩虹 《广东海洋大学学报》2001,21(4):42-46

胰蛋白酶的固定化采用吸附-交联法.在单因素试验基础上,通过正交试验,获得了胰蛋白酶固定化的最佳条件酶与载体体积比为21,吸附时间为10 h,pH=4.5,缓冲液浓度为0.05 mol/L,戊二醛的质量分数为0.1%.此条件下制得的磁性酶活力回收在50%左右,相对酶活力达74%,该磁性酶对啤酒澄清防止冷浑浊有明显效果.  相似文献   

九孔鲍养殖水体中产酶菌群的研究     

王志  蔡俊鹏  宋志萍  温其标 《广东海洋大学学报》2005,25(3):43-47

为了解九孔鲍养殖环境中细菌生理菌群的生态作用,对分离自养殖水体的33株菌株的产酶能力和种类进行了研究。结果表明,具有分泌胞外蛋白酶、脂肪酶和纤维素酶能力的菌株分别为39.4%、57.6%和30.3%;在这33株菌中,产至少一种胞外酶的菌株比例高达63.6%。API鉴定结果表明,在有能力分泌胞外蛋白酶、脂肪酶和纤维素酶的菌株中,弧菌菌群所占的比例分别为46%、42%和40%,而其中河流弧菌又占各弧菌菌群比例的83.3%、62.5%和100%。充分阐明弧菌,尤其是河流弧菌为优势菌,在鲍鱼养殖微生物态中起的重要作用。  相似文献   

A sulfotransferase specific to N-21 of gonyautoxin 2/3 from crude enzyme extraction of toxic dinoflagellate Alexandrium tamarense CI01     

王大志  张树刚  洪华生 《中国海洋湖沼学报》2007,25(2):227-234

Sulfotransferase (ST) is the first enzyme discovered in association with paralytic shellfish poisoning (PSP) toxin biosynthesis in toxic dinoflagellates. This study investigates the ST activity in crude enzyme extraction of a toxic dinoflagellate species, Alexandrium tamarense CI01. The results show that crude enzyme can transfer a sulfate group from 3’-phosphoadenosine 5’-phosphosulfate (PAPS) to N-21 in the carbamoyl group of gonyautoxin 2/3 (GTX2/3) to produce C1/C2, but is inactive toward STX to produce GTX5. The crude enzyme is optimally active at pH 6.0 and 15°C. The activity is enhanced by Co2 , Mg2 , Mn2 and Ca2 individually, but is inhibited by Cu2 . Moreover, the activity shows no difference when various sulfur compounds are used as sulfate donors. These results demonstrate that the ST specific to GTX2/3 is present in the cells of A. tamarense CI01 and is involved in PSP toxin biosynthesis. In addition, the ST from different dinoflagellates is species-specific, which explains well the various biosynthesis pathways of the PSP toxins in toxic dinoflagellates.  相似文献   

Towards the in situ measurement of the remanent magnetization of oceanic basalts     

L. Daly  A. Tabbagh† 《Geophysical Journal International》1988,95(3):481-489

  相似文献   

基于SWAT模型的汉江流域径流模拟   总被引：1，自引：0，他引：1  

夏智宏  周月华  许红梅 《气象》2009,35(9):59-67

应用SWAT(Soil and Water Assessment Tool)分布式水文模型对汉江流域1971-2000年30年逐月径流进行了模拟.结果表明:模型模拟精度高于评价标准(模拟效率Ens>0.5,相关系数r 2>0.6),SWAT模型适用于汉江流域的径流模拟;水量平衡各要素中,30年月、年平均蒸散发量、地表径流量、土壤对地下水补给量、土壤含水变化量、地下水侧流量分别占降水量的55.97%、25.88%、17.64%、0.26%、0.25%,蒸散发是该流域水量的主要输出项;各月30年平均降水量变化趋势与地表径流量变化趋势较一致,而与基流量变化趋势一致性较差;30年流域降水量年变化趋势与地表径流量、基流量的变化趋势较一致;30年月、年地表径流量对降水的响应程度高于基流.  相似文献   

越冬对池塘专养模式下中华鳖(Trionyx sinensis)机体形态、血清生化、脏器相关功能酶活力及肌肉和裙边物性的影响     

李有志  牟恩镟  申屠琰  朱卫东  赵蓓蓓  王志铮 《海洋与湖沼》2022,53(2):421-429

水生动物往往会通过调整自身生理生化特征而形成各自固有的越冬生存适应对策,因此,深入探究越冬对具有冬眠习性的目标水产养殖动物机体生理状况的影响过程与机制,对指导其安全养殖具重要参考价值。以池塘专养模式下2⁺龄中华鳖(Trionyx sinensis)同生群个体为研究对象,从机体形态、血清生化、脏器相关功能酶活力和肌肉与裙边物性等四个维度开展了中华鳖越冬前后生理生化特征的差异研究,以进一步了解越冬对中华鳖生理生化的影响特征。结果表明:(1)越冬期间实验中华鳖总体上呈现较明显的生长态势,所测30项体尺性状和12项质量性状测定值间的差异相似性分别为46.7%和8.3%;(2)所测16项血清生化指标中,越冬后显著降低的为TG等5项(P<0.05),显著增加的为TP等4项(P<0.05),无显著差异的为GLB等7项(P>0.05);(3)所测8项脏器酶学指标中,越冬后酶活力显著下降的为肝脏CAT、POD和脂肪酶(P<0.05),显著升高的为胃蛋白酶等相关酶指标(P<0.05);(4)所测7项物性指标中,越冬后裙边弹性和咀嚼性均显著增加(P<0.05),其余为下降或无显著差异(P>0.05)。表明越冬为引起肝脏功能发生正常切换的重要时期,但不会引起心肝功能产生实质性改变。研究结果可为中华鳖越冬生理生态学研究和池养中华鳖越冬管理技术体系构建提供基础资料。  相似文献   

福建省贝藻类养殖碳汇及其潜力评估          下载免费PDF全文

张麋鸣  颜金培  叶旺旺  张超  高众勇  徐长安  李玉红  张介霞  赵淑惠  孙恒  詹力扬 《应用海洋学学报》2022,41(1):53-59

海水贝藻类养殖碳汇是海洋渔业碳汇的重要组成,开展其碳汇研究对服务我国实现碳中和目标有积极的作用.福建省的海水养殖规模和产量居于全国前列,对其海水贝藻类养殖碳汇及潜力评估有重要的意义.本研究分析了2015至2020年度《中国渔业统计年鉴》海水贝藻类养殖数据,估算了2014年至2019年全国及福建省海水贝藻类养殖碳汇强度,...  相似文献   

中药资源地理国情监测工具集设计与实现     

张春星  霍亮  靖常峰 《测绘与空间地理信息》2017,(2):152-155

目前第四次全国中药资源普查试点工作采集了大量的成果数据,并且成果数据与地理空间位置之间存在天然的联系。本文以中药资源数据的空间属性作为出发点,在中药资源普查成果数据和已有地理国情监测数据的基础上,建立了基础地理数据库、专题地理数据库和中药资源数据库,设计并实现了能够进行中药资源专题分析计算的地理工具集。最后,基于B/S架构完成了中药资源地理国情监测技术平台的构建,验证了地理工具集设计思路的正确性。  相似文献   

基于ArcGIS建模的基本农田数据库建库方法探讨     

吴志娟 《测绘与空间地理信息》2017,40(9)

通过图形化建模工具Model Builder对基本农田数据进行建库,是通过构造地理工作流和脚本将数据和空间处理工具连接起来处理的复杂的GIS任务。本文主要对基本农田数据库的建库采用Arc GIS建模的方法进行了探讨。  相似文献   

Learning from CDM SD tool experience for Article 6.4 of the Paris Agreement     

Karen Holm Olsen  Christof Arens  Florian Mersmann 《Climate Policy》2018,18(4):383-395

The Paris Agreement (PA) emphasizes the intrinsic relationship between climate change and sustainable development (SD) and welcomes the 2030 agenda for the global Sustainable Development Goals (SDGs). Yet, there is a lack of assessment approaches to ensure that climate and development goals are achieved in an integrated fashion and trade-offs avoided. Article 6.4 of the PA introduces a new Sustainable Mitigation Mechanism (SMM) with the dual aim to contribute to the mitigation of greenhouse gas emissions and foster SD. The Kyoto Protocol’s Clean Development Mechanism (CDM) has a similar objective and in 2014, the CDM SD tool was launched by the Executive Board of the CDM to highlight the SD benefits of CDM activities. This article analyses the usefulness of the CDM SD tool for stakeholders and compares the SD tool’s SD reporting requirements against other flexible mechanisms and multilateral standards to provide recommendations for improvement. A key conclusion is that the Paris Agreement’s SMM has a stronger political mandate than the CDM to measure that SD impacts are ‘real, measurable and long-term’. Recommendations for an improved CDM SD tool are a relevant starting point to develop rules, modalities, and procedures for SD assessment in Article 6.4 as well as for other cooperative mitigation approaches.

POLICY RELEVANCE

Research findings are relevant for developing the rulebook of modalities and procedures for Article 6.4 of the Paris Agreement, which introduces a new mechanism for mitigation of greenhouse gas emissions and sustainable development. Lessons learnt from the CDM SD tool and recommendations for enhanced SD assessment are discussed in context of Article 6 cooperative approaches, and make a timely contribution to inform negotiations on the rulebook agreed by the Conference of the Parties serving as the Meeting of the Parties to the Paris Agreement.  相似文献