基于UPLC-Q-Exactive MS构建麻痹性贝类毒素的特征指纹溯源技术          下载免费PDF全文

张帆  郑关超  王潇潇  翟毓秀  谭志军  吴海燕 《海洋与湖沼》2024,55(1):135-144

麻痹性贝类毒素污染是世界共同关注和重点管控的食品安全问题, 其溯源一直是产业监管的难点和技术重点。基于指纹溯源技术理论, 采用室内模拟3种产毒藻暴露紫贻贝后风险形成过程, 开发基于超高效液相色谱-四极杆-静电场轨道阱高分辨质谱(UPLC-Q-Exactive MS)鉴定麻痹性贝类毒素区域特征的指纹溯源技术。结果表明, 采用乙腈/甲醇/丙酮(体积比为1︰1︰1)混合溶液作为提取剂, C8色谱柱联合正离子模式, 质荷比(m/z) 100~600和Amide色谱柱联合正离子模式, m/z 600~1 500作为色谱质谱检测条件下, 提取的化合物数量较多且稳定性良好, 覆盖了40.4%指纹信息; 紫贻贝与产毒藻中的共检出11种毒素成分, 且具有高度相关性。N-磺酰胺甲酰基类毒素(如C类)和膝沟藻毒素(GTX类)在暴露期发生了显著的代谢转化; 将摄食3种产毒藻后紫贻贝的差异化合物与特征毒素组分进行主成分分析及正交偏最小二乘法判别分析, 共筛选出13种复合指纹物质。构建6种特征物质的Fisher判别模型, 交叉验证准确率为88.9%, 可实现产毒藻种溯源。该研究建立了基于超高效液相色谱-四极杆-静电场轨道阱高分辨质谱UPLC-Q-Exactive MS紫贻贝特征指纹溯源技术, 初步实现了从污染麻痹性贝类毒素的贝类到肇事藻种的逆向溯源,可应用于贻贝麻痹性贝类毒素风险溯源研究。  相似文献   

An individual-based population dynamic model for estimating biomass yield and nutrient fluxes through an off-shore mussel (Mytilus galloprovincialis) farm     

Daniele Brigolin  Gabriele Dal Maschio  Federico Rampazzo  Michele Giani  Roberto Pastres 《Estuarine, Coastal and Shelf Science》2009

The fluxes of carbon, nitrogen and phosphorus through an off-shore long-line Mytilus galloprovincialis farm during a typical rearing cycle were estimated by combining a simple population dynamic model, based on a new individual model, and a set of field data, concerning the composition of the seston, as well as that of mussel meat and faeces. The individual model, based on an energy budget, was validated against a set of original field data, which were purposely collected from July 2006 to May 2007 in the North-Western Adriatic Sea (Italy) and was further tested using historical data. The model was upscaled to the population level by means of a set of Monte Carlo simulations, which were used for estimating the size structure of the population. The daily fluxes of C, N and P associated with mussel filtration, excretion and faeces and pseudo-faeces production were integrated over the 10-month-long rearing cycle and compared with the total amount of C, N and P removed by harvesting. The results indicate that the individual model compares well with an existing literature model and provides reliable estimations of the growth of mussel specimen over a range of trophic conditions which are typical of the Northern Adriatic Sea coastal area. The results of the budget calculation indicate that, even though the harvest represents a net removal of phosphorus and nitrogen from the ecosystem, the mussel farm increases the retention time of both nutrients in the coastal area, via the deposition of faeces and pseudo-faeces on the sea-bed. In fact, the amount of nitrogen associated with deposition is approximately twice the harvested one and the amount of phosphorus is approximately five times higher. These findings are in qualitative agreement with the results of literature budget and model calculations carried out in a temperate coastal embayment. This agreement suggests that the proper assessment of the overall effect of long-line mussel farming on both the benthic and pelagic ecosystem asks for an integrated modelling approach, which should include the dynamic of early diagenesis processes, as well as of that of nutrients released from the surface sediment.  相似文献   

Rheological properties and structural changes in different sections of boiled abalone meat   总被引：1，自引：0，他引：1  

Ogawa Hiroo 《中国海洋大学学报(英文版)》2003,2(1):44-48

1　Introduction　AbaloneHaliotisdiscusisaverypopularfoodthrough outtheworld .WhenAsianpeoples ,notablyJapaneseandChinese ,eatabalonedishes ,theyenjoythefirmtextureoftherawmeatandthetendernessoftheboiledmeat .InJapan ,thetoughervarietiesoftherawabalonemeatareenjoyedandtheabaloneisoftenthin lyslicedandservedraw (sashimi) ,whileinchina ,thetendernessandsoftervarietiesoflesscollagenofboiledmeatareconsideredsuitableforeating ,sotheabaloneisoftenservedbyheating .Infoodscience ,manyreportshavebeen …  相似文献   

IMPACT OF STARVATION ON SURVIVAL，MEAT CONDITION AND METABOLISM OF CHLAMYS FARRERI     

杨红生 王健周毅  王萍何义朝  张福绥 《中国海洋湖沼学报》2001,19(1):51-56

The effects of 60-day starvation on survival rate. condition index (CI) , changes ofnutrient composition of different tissues, respiration and excretion of scallop ChlartnCs farreri were studiedin laboratory from Oct. 17 to Dec. 15 ,1997. Two groups (control and starvation with 200 individualseach) were cultured in two 2 m3 tanks, with 31 to 32 salinity water at 1 7 “C . Starvation effects were mea-sured alter 1 0, 20, 40 and 60 days. There was no mass mortality of scallops cf the two tanks and survivalrates of the control and starvation groups were 93 .5 % and 92.0 %, respectively. Starvation had strongeffect on the meat condition of the scallops, especially after 10 days; when relative lipid percentagedropped sharply while relative protein percentage increased. The impact of starvation on the oxygen consumption mtc (OCR) and the ammonia-N cxcretion mtc (AER) was obvious.  相似文献   

Microbial modulation in the biomass and toxin production of a red-tide causing alga   总被引：6，自引：0，他引：6  

Zheng TL  Su JQ  Maskaoui K  Yu ZM  Hu Z  Xu JS  Hong HS 《Marine pollution bulletin》2005,51(8-12):1018-1025

The effect of S₁₀, a strain of marine bacteria isolated from sediment in the Western Xiamen Sea, on the growth and paralytic shellfish poison (PSP) production in the alga Alexandrium tamarense (A. tamarense) was studied under controlled experimental conditions. The results of these experiments have shown that the growth of A. tamarense is obviously inhibited by S₁₀ at high concentrations, however no evident effect on its growth was observed at low concentrations. Its PSP production was also inhibited by S₁₀ at different concentrations, especially at low concentrations. The toxicity of this strain of A. tamarense is about (0.95–12.14) × 10⁻⁶ MU/cell, a peak toxicity value of 12.14 × 10⁻⁶ MU/cell appeared on the 14th day, after which levels decreased gradually. The alga grew well in conditions of pH 6–8 and salinities of 20–34‰. The toxicity of the alga varied markedly at different pH and salinity levels. Toxicity decreased as pH increased, while it increased with salinity and reached a peak value at a salinity of 30‰, after which it declined gradually. S₁₀ at a concentration of 1.02 × 10⁹ cells/ml inhibited growth and the PSP production of A. tamarense at different pH and salinity levels. S₁₀ had the strongest inhibitory function on the growth of A. tamarense under conditions of pH 7 and a salinity of 34‰. The best inhibitory effect on PSP production by A. tamarense was at pH 7, this inhibitory effect on PSP production did not relate to salinity. Interactions between marine bacteria and A. tamarense were also investigated using the flow cytometer technique (FCM) as well as direct microscope counting. S₁₀ was identified as being a member of the genus Bacillus, the difference in 16S rDNA between S₁₀ and Bacillus halmapalus was only 2%. The mechanism involved in the inhibition of growth and PSP production of A. tamarense by this strain of marine bacteria, and the prospect of using it and other marine bacteria in the bio-control of red-tides was discussed.  相似文献   

Fate of paralytic shellfish poisoning toxins in purple clam Hiatula rostrata,in outdoor culture and laboratory culture   总被引：1，自引：0，他引：1  

Chen CY  Chou HN 《Marine pollution bulletin》2002,44(8):733-738

Purple clams (Hiatula rostrata Lighttoot) accumulate paralytic shellfish poisoning (PSP) toxins produced by a toxic strain of the dinoflagellate Alexandriun minutum Halim. The results confirm the data of our previous study concerning the muscle and siphon that were not showing a gradual rise in toxicity when shellfish accumulated more A. minutum. However, muscle and siphon are intermittently toxic both in exposure and depuration period in laboratory cultured purple clams. PSP toxins were detected in outdoor cultured purple clams, whereas no A. minutum were found in the culture pond during most of the survey time. The outdoor cultured purple clams need longer time to decrease toxicity to allowable levels than laboratory cultured purple clams. It was shown that laboratory data may not predict times over which pond-cultured purple clams may prove toxic to consumers.  相似文献   

我国海洋鱼类和贝类染色体组型研究进展   总被引：7，自引：1，他引：6  

王梅林  郑家声  朱丽岩  戴继勋 《中国海洋大学学报(自然科学版)》2000,30(2):277-284

综述迄今为止已报道的我国 38种海洋鱼类和 2 2种海洋贝类的染色体组型 ,以及其中 6种鱼和 1种贝的 C-带、Ag- NOR带等细胞遗传学研究的结果 ,并对这项研究工作存在的问题进行了探讨并对未来作了展望。  相似文献   

三门湾主要经济贝类胃含物中微、小型藻类组成     

朱根海  丰卫华 《海洋科学》2020,44(1):97-105

本文对采自三门湾泥滩、沙滩、岩礁潮间带的主要经济贝类彩虹明樱蛤、缢蛏、僧帽牡蛎、青蚶、光滑河篮蛤胃含物中的微、小型藻类组成进行了鉴定分析。结果表明, 5种经济贝类胃含物中共鉴定出微、小型藻类143种,其中,硅藻占75.5%;甲藻占12.6%;绿藻门和金藻门占5.6%;蓝藻门和隐藻占4.2%;其他类占2.1%。胃含物中出现的细胞个体中,小于20μm的主要微型藻类是中肋骨条藻、盔状舟形藻、尤氏直链藻、小环藻、海链藻等;细胞个体大于20μm的主要小型藻类是琼氏圆筛藻、海生斑条藻、短柄曲壳藻、钝角齿状藻等。  相似文献   

UHPLC-LTQ-Orbitrap-MS非定向筛查贝类中氮杂螺环酸毒素及其代谢产物     

吴海燕  陈佳琦  董晨帆  王松  崔海栋  谭志军 《海洋与湖沼》2020,51(6):1432-1439

为了分析复杂贝类和微藻中的氮杂螺环酸毒素（AZAs）及其代谢产物，建立一种基于超高效液相色谱-线性离子阱/静电场轨道阱组合式高分辨质谱（UHPLC-LTQ-Orbitrap-MS）联用技术的非定向筛查分析方法。采用Full MS/dd MS²、PRM和Target-SIM/dd MS²三种质谱鉴定模式，整合多种数据采集挖掘策略，通过研究多种AZAs在不同基质样品中的质谱裂解规律及特征碎片离子丰度，实现AZAs及其衍生物的快速筛查检测和精准鉴别。结果表明：PRM模式能有效排除AZAs同分异构体的干扰，获得更高的专属性，适用于目标代谢物筛查；应用Full MS/dd MS²和PRM结合的方式，根据AZAs裂解途径及多种AZAs代谢产物裂解规律，在贝类中共鉴别出20种AZAs系列化合物，其中包括初步推测了3种新型AZAs代谢产物的结构。应用本方法还发现AZA9、AZA10、AZA19等代谢物均随代谢过程持续升高，是AZA2在贝类代谢过程中的末端产物。该方法能够为复杂基质中的AZAs系列毒素及其衍生常规检测与精准鉴别提供参考，可应用于解析AZAs毒素在贝类体内的代谢转化机制研究。  相似文献   

Dinophysis caudata generated lipophilic shellfish toxins in bivalves from the Nanji Islands,East China Sea   总被引：1，自引：0，他引：1  

江涛  徐轶肖  李扬  齐雨藻  江天久  吴锋  张帆 《中国海洋湖沼学报》2014,32(1):130-139

A 12-month program of monitoring potentially toxic microalgae(that produce lipophilic shellfi sh toxins; LSTs) and their toxins in bivalves was conducted from April 2006 to March 2007 in the Nanji Islands, East China Sea. Two Dinophysis species, D. caudata and D. acuminata, were identifi ed, and D. caudat a was found to be the dominant species. D. caudata was detected in water samples between April and June 2006, and between February and March 2007. It reached its highest abundances in May, with a mean abundance of 1.38×10 2 cells/L in surface water and 1.25×10 2 cells/L in bottom water(10 m deep). The temporal distribution of D. caudata was associated with the occurrence of LSTs in bivalve samples, which mostly occurred at the same time as D. caudata blooms, between April and July 2006. All of the cultured bivalves sampled between April and June were contaminated with LSTs, with an average toxicity of 85 μg okadaic acid(OA) eq./100 g meat, which was four times higher than the Chinese regulatory limit(20 μg OA eq./100 g meat). Ten out of fi fteen wild samples(66.7%) collected during the same period were positive for LSTs, and contained an average LST toxicity of 45 μg OA eq./100 g meat(more than twice the regulatory value). Cultured Patinopecten yessoensis collected on 15 May 2006 had the highest toxicity, 320 μg OA eq./100 g meat, and relatively high toxicities(80 to 160 μg OA eq./100 g meat) were found in bivalves until the end of July.  相似文献