Drying of Micro-Encapsulated Lactic Acid Bacteria- Effects of Trehalose and Immobilization on Cell Survival and Release Properties     

LI Xiaoyan  CHEN Xiguang 《青岛海洋大学学报(英文版)》2009,(1):39-44

Lactic acid bacteria （LAB） were encapsulated with alginate, gelatin and trehalose additives by the extrusion method and dried at 4 ℃. The microcapsules were generally spherical and had a wrinkled surface with a size of 1.7mm± 0.2mm Trehalose as a carbohydrate source in the culture medium could reduce acid production and performed no function in the positive proliferation of LAB. Using trehalose as a carbohydrate source and protective medittm simultaneously had a benefit in the protection of LAB cells during the storage at 4 ℃. The density of live LAB cells could be 10^7 CFU g^-1 after 8weeks of storage. Cells of LAB could be continuously released from the capsules from the acidic （pH 1.2） to neutral conditions （pH 6.8）. The release amounts and proliferation speeds of LAB cells in neutral medium were much larger and faster than those in acidic conditions. Additionally, nxtmobilization of LAB could improve the survival of cells when they were exposed to acidic medium （pH 1.2） with a survival rate of 76%.  相似文献   

赤潮藻类温度生态幅的定量表达模型研究   总被引：4，自引：0，他引：4  

陈艳拢  赵冬至  杨建洪  赵玲 《海洋学报》2009,31(5):156-161

首先在Moisan的温度和比生长率关系基础上,依据Shelford耐受性定律,建立了赤潮藻类温度生态幅(最适温度、适温范围、耐受范围)的定量表达模型。同时,依据此模型可定量导出赤潮藻类的生物学零度。其次,结合实验培养数据,建立了东海原甲藻比生长率的温度响应模式,并利用研究结果定量得到该藻种的温度生态幅,这与已有的实验研究和现场监测结果有较好的一致性。  相似文献   

鹿道口高盐环境中一株溶血性葡萄球菌分离、鉴定与生理特征     

黄增金  汪城墙  尹腾飞  张小葵  刘爱秋 《海洋湖沼通报》2009,(4)

试验从威海市羊亭镇鹿道口盐场筛选、纯化了1种性状优良的菌株Staphylococcus haemolyticus wh-2,该菌株具有较强的生长代谢的能力,在多方面都表现优良,具有抗裂解,抗低温等性质,在不同逆境环境下培养与观察,认识到微生物在不同逆境环境下的生理多样性,是1株有优良特征的资源菌株.经16SrDNA鉴定分析,鉴定为溶血性葡萄球菌(Staphylococcus haemolyticus wh-2).  相似文献   

Effect of low salinity on the survival of recently hatched veliger of Nassarius reticulatus (L.) in estuarine habitats: A case study of Ria de Aveiro     

Luciana Gnio  Ana Sousa  Nuno Vaz  Joo M. Dias  Carlos Barroso 《Journal of Sea Research》2008,59(3):133-143

The effect of low salinity on the survival of recently hatched veliger of the gastropod Nassarius reticulatus (L.) was studied under laboratory conditions at 17 °C ± 1 °C. Significant mortality occurred for salinities ≤ 17 psu and the LS₅₀ (salinity that causes 50% mortality) varied from 14.5 psu to 17.2 psu for exposures between 1 h and 48 h, respectively. The results were included in a mathematical model in order to estimate the mortality of N. reticulatus veliger in Ria de Aveiro under different salinity scenarios determined by numerical simulation. The model predicts a massive mortality of veliger immediately after their eclosion (1 h) in Ria de Aveiro during spring tide when the river input reaches its expected maximum — a situation that has been observed occasionally and may overlap a massive eclosion of veliger in the water. Except in this extreme situation, the mortality in Ria de Aveiro is generally restricted to the upstream limits of the channels. The results indicate that N. reticulatus recruitment in estuaries may strongly depend on the prevailing salinity regime.  相似文献   

屏障效应对生物地球化学异常形成的影响及找矿有效指示植物的选择     

宋慈安  雷良奇  杨仲平  文宗振 《地质科技情报》2012,31(1):1-8

采用光谱仪及透射电子显微镜对广西几个金属矿床的生物地球化学异常和植物细胞微观特征进行了研究,结果发现:生物地球化学异常对土壤地球化学异常有明显的物质性继承关系;生物地球化学异常的物质以金属聚集体(电子致高密度体物质)的形式沉淀在植物液胞中或细胞壁及膜边缘,其对植物的伤害可能只达到细胞或组织的水平.生物地球化学异常的形成...  相似文献   

Effect of soil salinity on cold tolerance of mangrove Kandelia candel     

杨盛昌  李云波林鹏 《中国海洋湖沼学报》2005,23(1):98-103

Analysis of cold tolerance on mangrove Kandelia candel leaf growing in different soil salinity along Jiulong River Estuary in South China showed that the cold tolerance decreased as the increase of soil salinity. The lethal temperatures of K. candel leaf were-10.4,-9.9 and-8.6℃ in Liaodong, Baijiao and Aotou, respectively. Under 1-2℃ cold stress treatment on detached leaves of K. candel, their caloric value gradually decreased, while electrolyte leakage gradually increased. The leaf＇s caloric value and electrolyte leakage in Aotou with higher soil salinity varied more largely than those in Liaodong with lower soil salinity. In K. candel leaf, total water content lowered a little, bound water content rose significantly and free water content dropped significantly with duration of cold stress. At the same time, reduction sugar, soluble sugar and starch content gradually decreased and sucrose content gradually increased. Bound water, free water and sucrose content in K. candel leaf from Aotou with higher soil salinity changed more slowly than those from Liaodong with lower soil salinity, but reduction sugar, soluble sugar and starch content in K. candel leaf from Aotou had faster variations than those from Liaodong. These data indicated that soil salinity can reduce cold tolerance of K.candel leaf by increasing negative effect of salt ions in cell membrane, inhibiting variations of water content, and aggravating consumption of material and energy.  相似文献   

Uncertainties in global warming science and near-term emission policies     

L. D. Danny Harvey 《Climate Policy》2013,13(5):573-584

Abstract

It is argued here that stringent, early emission reductions are necessary in order to minimize ‘dangerous anthropogenic interference in the climate system’ (DAI), the stated Objective of Article 2 of the UNFCCC (United Nations Framework Convention on Climate Change). Given probability distribution functions (pdfs) for climate sensitivity and the temperature threshold for harm consistent with currently available evidence, and accepting a 10% risk of unacceptable damage as the threshold for ‘danger’, it is not possible to avoid DAI. Having adopted a precautionary approach in setting emission trajectories, the possibility arises that future resolution of uncertainties concerning climate sensitivity and the harm threshold may show the climate sensitivity to be low (1–2 K) and the harm threshold high (2 K rather than 1 K). Using a simple coupled climate-carbon cycle model, it is shown that if the climate sensitivity were to be definitively determined to be 2 K in 2020, then the emission reductions achieved by that time and planned for the next two decades are still fully needed. Only if climate sensitivity is very low (1 K) and the harm threshold is high (2 K) would the emissions achieved by 2020 not have been fully necessary. However, this would still lead to changes in ocean chemistry that are likely to be highly detrimental to marine life. Thus, when the full spectrum of impacts is considered, there is no plausible set of assumptions under which stringent near-term emission reductions are rendered unnecessary.  相似文献   

Fe^2＋浓度对Acidithiobacillus ferrooxidans耐铜性的影响   总被引：1，自引：0，他引：1  

符翔  王红梅  刘乔  周健平 《矿物学报》2011,31(4):662-667

嗜酸性氧化亚铁硫杆菌（Acidithiobacillus ferrooxidans）是铜矿生物冶金中应用最广泛的微生物之一。但在冶金过程中淋滤出来的Cu2＋等重金属逐渐积累,达到一定的浓度后就会抑制A.ferrooxidans的生长,从而降低冶金的效率。本文着重研究了Fe2＋初始浓度对A.ferrooxidans耐铜性的影响。实验中ρ（Cu2＋）变化范围为0～5.0 g/L。结果表明,当ρ0（Fe2＋）为6.7 g/L时,A.ferrooxidans仅在Cu2＋为0～0.4 g/L的体系中能显著地氧化Fe2＋进行生长;当Cu2＋≥0.5 g/L时,A.ferrooxidans生长完全受到抑制。将ρ0（Fe2＋）增加到8.9 g/L,A.ferroox-idans在0.5、1.0、2.0和3.0 g/L Cu2＋的培养基中也能明显氧化Fe2＋,并最终将其完全氧化,Cu2＋对A.ferrooxidans生长抑制作用仅出现在4.0和5.0 g/L Cu2＋的体系中。因此提高体系中亚铁离子的浓度能提高菌体对Cu2＋的耐受力。研究结果对铜矿的生物冶金具有重要意义。  相似文献   

条纹锯鮨对盐度胁迫的耐受力及淡水胁迫恢复的初步研究   总被引：2，自引：0，他引：2  

阳钢  刘超  马甡  王成刚  王志军 《海洋湖沼通报》2011,(2):73-78

本文研究了条纹锯鮨(Centropristis striata)鱼种对低盐度胁迫的耐受力,以及淡水胁迫后最适恢复时间范围.实验结果表明:规格(20.32±9.67)cm的鱼种在0盐度的半致死时间为(1.63±0.23)h,规格(10.20±0.63)cm的鱼种在0盐度的半致死时间为(0.77±0.23)h.同时,2种规...  相似文献   

Effect of soil salinity on cold tolerance of mangrove Kandelia candel     

Yang Shengchang  Li Yunbo  Lin Peng 《中国海洋湖沼学报》2005,23(1):98-103

Analysis of cold tolerance on mangroveKandelia candel leaf growing in different soil salinity along Jiulong River Estuary in South China showed that the cold tolerance decreased as the increase of soil salinity. The lethal temperatures ofK. candel leaf were −10.4, −9.9 and −8.6 °C in Liaodong, Baijiao and Aotou, respectively. Under 1–2°C cold stress treatment on detached leaves ofK. candel, their caloric value gradually decreased, while electrolyte leakage gradually increased. The leaf's caloric value and electrolyte leakage in Aotou with higher soil salinity varied more largely than those in Liaodong with lower soil salinity. InK. candel leaf, total water content lowered a little, bound water content rose significantly and free water content dropped significantly with duration of cold stress. At the same time, reduction sugar, soluble sugar and starch content gradually decreased and sucrose content gradually increased. Bound water, free water and sucrose content inK. candel leaf from Aotou with higher soil salinity changed more slowly than those from Liaodong with lower soil salinity, but reduction sugar, soluble sugar and starch content inK. candel leaf from Aotou had faster variations than those from Liaodong. These data indicated that soil salinity can reduce cold tolerance ofK. candel leaf by increasing negative effect of salt ions in cell membrane, inhibiting variations of water content, and aggravating consumption of material and energy. Supported by the Found for Outstanding Young Teacher, the Ministry of Education, China.  相似文献