White spot syndrome virus inactivation study by using gamma irradiation     

Marzieh Heidareh  Farahnaz Motamedi Sedeh  Mehdi Soltani  Saeed Rajabifar  Mohammad Afsharnasab  Aghil Dashtiannasab 《中国海洋湖沼学报》2014,32(5):1024-1028

The present study was conducted to investigate the effect of gamma irradiation on white spot syndrome virus(WSSV). White spot syndrome virus is a pathogen of major economic importance in cultured penaeid shrimp industries. White spot disease can cause mortalities reaching 100% within 3–10 days of gross signs appearing. During the period of culture, immunostimulant agents and vaccines may provide potential methods to protect shrimps from opportunistic and pathogenic microrganisms. In this study, firstly, WSSV was isolated from infected shrimp and then multiplied in crayfish. WSSV was purified from the infected crayfish haemolymph by sucrose gradient and confirmed by transmission electron microscopy. In vivo virus titration was performed in shrimp, Penaeus semisulcatus. The LD50 of live virus stock was calculated 10 5.4 /mL. Shrimp post-larvae(1–2 g) were treated with gamma-irradiated(different doses) WSSV(10 0 to 10-4 dilutions) for a period of 10 days. The dose/survival curve for irradiated and un-irradiated WSSV was drawn; the optimum dose range for inactivation of WSSV and unaltered antigenicity was obtained 14–15 kGy. This preliminary information suggests that shrimp appear to benefit from treatment with gammairradiated WSSV especially at 14–15 KGy.  相似文献   

牙鲆迟缓爱德华氏菌急性感染实验:4种不同方法的比较   总被引：3，自引：0，他引：3  

刘云  刘允坤  杨栋  姜国良 《海洋科学》2001,25(2):8-11

为了检验疫苗对牙鲆免疫效果，探讨有效的注射途径尤为重要，本文通过采用迟缓爱德华氏菌(Edwardsiella tarda)对牙鲆（Paralicthys oliwaceus)进行感染实验，对口腔、肌肉、腹腔和腹腔加肌肉４种注射方法了评价比较，其半致死浓度（ＬＤ５０）分别为口腔灌注法１０^8.38 CFU/ml、肌肉注射法１０^7.54CFU/ml、腹腔注射法１０^6.70CFU/ml和腹腔加肌肉注射法１０^6.09CFU/ml。结果表明牙鲆对迟缓爱德华氏菌敏感，在这４种注射方法中，以腹腔注射法这种人工方式最佳。  相似文献   

基于LD与LCS的1：50000正射影像元数据的自动匹配     

周治武  王发良  朱秀丽  朱杰 《测绘软科学研究》2012,(2):30-32

对多源、异构的正射影像元数据进行规范化褴合处理是闭家1：50000正射影像库建设中的一项重要内容。本文针对1：50000正射影像元数据的特征,提出了基于LD与LCS综合评价算法的自动匹配流程、模型和方法,高效地实现了元数据的自动匹配。  相似文献   

文昌鱼对几种环境因子及污染物的耐受极限初步研究   总被引：1，自引：0，他引：1  

白秀娟  卢伙胜  廖春风 《广东海洋大学学报》2007,27(1):92-94

<正>文昌鱼(Branchiostoma belcheri)隶属脊索动物门头索动物亚门,是全世界现存动物中与脊椎动物关系最近的一类非脊椎动物[1],被称为生物进化的“活化石”。它营养丰富,干品蛋白质含量约达70%左右,还含有较高的磷和碘,脂肪含量低,具有较高的  相似文献   

渤海海域旅大21-A构造沙四段储集层特征及控制因素     

刘志刚  杜晓峰  王清斌  王军  王晓刚  郭涛 《海洋地质译丛》2012,(2):26-29,89

渤海海域旅大21-A构造在3600m以下的沙四段中发现了很好的油气显示,并且测试成功。沙四段在该构造是个新的油气勘探层系,具有很好的勘探前景。为了更好地研究该构造储集层情况,通过对该构造沙四段岩心、壁心资料,运用常规、铸体薄片鉴定和扫描电镜等化验分析资料,对该构造沙四段储集层基本特征进行了系统分析,认为该构造岩性主要以长石岩屑砂岩为主,岩屑长石砂岩次之,孔隙质量为中等一好,渗透率为特低渗～中渗,储集层物性中等。确定了影响储集层物性的主要因素为沉积作用、成岩作用和构造作用,三者综合影响着储集层的物性变化。  相似文献   

Identification and characterization of the Vibrio anguillarum prtV gene encoding a new metalloprotease     

莫照兰  郭东升  茅云翔  叶旭红  邹玉霞  肖鹏  郝斌 《中国海洋湖沼学报》2010,(1)

We cloned and sequenced a prtV-like gene from Vibrio anguillarum M3 strain.This prtV gene encodes a putative protein of 918 amino acids,and is highly homologous to the V.cholerae prtV gene.We found that a prtV insertion mutant strain displayed lower gelatinase activity on gelatin agar,lower protease activity against azocasein,and lower activity for four glycosidases.This prtV mutant strain also had increased activity for two esterases in its extracellular products,as analyzed by the API ZYM system.In additi...  相似文献   

来凤咸丰区块LD2井五峰-龙马溪组页岩发育特征及含气性分析   总被引：1，自引：0，他引：1  

姜生玲 《地质与勘探》2018,54(1):203-210

来凤咸丰区块位于湘鄂西褶冲带。页岩气参数井LD2井的钻探揭示了来凤咸丰区块两河口向斜翼部五峰-龙马溪组页岩发育特征和含气性特点,通过有机地球化学、X衍射全岩矿物、扫描电镜以及现场解析等实验方法手段,明确了LD2井五峰-龙马溪组页岩有机碳含量较高(TOC:0.42%~3.20%)、热演化程度适中(Ro:1.47%~2.63%)、矿物成分以碎屑岩脆性矿物为主(石英+长石:37.4%~65.3%)、特低孔特低渗的特征。通过与位于两河口向斜轴部的LD1井对比研究,认为两口井页岩品质大致相当,但LD2井含气量明显偏低,最大含气量仅0.72m~3/t,分析认为LD2井在新滩组钻遇断层,且龙马溪组顶界距离断层的横向距离不足20m,保存条件被破坏是导致LD2井五峰-龙马溪组页岩含气量偏低的主要原因。  相似文献