FOOD SOURCES AND CARBON BUDGET OF CHINESE PRAWN PENAEUS CHINENSIS     

董双林  张硕  王芳 《中国海洋湖沼学报》2002,(1)

ＩＮＴＲＯＤＵＣＴＩＯＮＰｒａｗｎｆａｒｍｉｎｇｉｎＣｈｉｎａｉｓａｎｉｍｐｏｒｔａｎｔｍａｒｉｃｕｌｔｕｒｅｉｎｄｕｓｔｒｙｗｈｏｓｅａｎｎｕａｌｐｒｏｄｕｃｔｉｏｎｈａｓｅｖｅｒｒｅａｃｈｅｄｔｏｍｏｒｅｔｈａｎ 2 .0× 1 0 5 ｔ,ｉｎｗｈｉｃｈｓｅｍｉ ｉｎｔｅｎｓｉｖｅｃｕｌｔｕｒｅｏｆＣｈｉｎｅｓｅｐｒａｗｎ (ＰｅｎａｅｕｓｏｒｉｅｎｔａｌｉｓＫｉｓｈｉｎｏｕｙｅ)ｃｏｎｔｒｉｂｕｔｅｄｔｈｅｇｒｅａｔｅｓｔｐａｒｔ.Ｂｕｔｉｎｒｅｃｅｎｔｙｅａｒｓｔｈｅｐｒａｗｎｃｕｌｔｕｒｅｉｎｄｕｓｔ…  相似文献   

中国对虾卵母细胞发育的初步研究   总被引：17，自引：0，他引：17  

李怀梅  张乃禹 《海洋与湖沼》1994,25(3):243-247

于１９８９年１０月－１９９０年９月分别先后在昌邑县下营镇对虾育苗场和青岛黄岛盐业公司养殖场采集中国对虾，运用光学，电子显微镜及组化方法，研究中国对虾卵母细胞的形态发育及其内部结构分化。结果表明，中国对虾卵母细胞的发育可分为４个主要时相：Ⅰ．早期分化时相，Ⅱ滤泡初生期时相，Ⅲ卵黄大量生成时相，Ⅳ皮质棒生成时相。  相似文献   

Purification and identification of a clotting protein from the hemolymph of Chinese shrimp (Fenneropenaeus chinensis)     

WANG Baojie  PENG Hongni  LIU Mei  JIANG Keyong  ZHANG Guofan  WANG Lei 《中国海洋大学学报(英文版)》2013,12(3):477-483

The clotting protein (CP) plays important and diverse roles in crustaceans, such as coagulation and lipid transportation. A clotting protein was purified from the hemolymph of Chinese shrimp Fenneropenaeus chinensis (named as Fc-CP) with Q sepharose HP anion-exchange chromatography and phenyl sepharose HP hydrophobic interaction chromatography. Fc-CP was able to form stable clots in vitro in the presence of hemocyte lysate and Ca²⁺, suggesting that the clotting reaction is catalyzed by a Ca²⁺-dependent transglutaminase in shrimp hemocytes. The molecular mass of Fc-CP was 380 kDa under non-reducing conditions and 190 kDa under reducing conditions as was determined with SDS-PAGE. CP exists as disulfide-linked homodimers and oligomers. The N-terminal amino acid sequence of Fc-CP was identical to that of shrimps including Penaeus monodon, Farfantepenaeus paulensis and Litopenaeus vannamei; and similar to that of other decapods. The purified Fc-CP was digested with trypsin and verified on an ABI 4700 matrix-assisted laser desorption/ionization tandem time-of-flight (MALDI-TOF/TOF) mass spectrometry. Our results will aid to better understanding the coagulation mechanism of shrimp hemolymph.  相似文献   

中华白海豚SRAP分子标记多态性的初步研究     

赵丽媛  陶翠花  许敏  祝茜 《台湾海峡》2012,31(2):195-201

首次将SRAP(sequence-related amplified polymorphism)方法引入中华白海豚(Sousa chinensis)多态性研究中,利用高分辨率的毛细管凝胶电泳分析了模板DNA、Mg2+、dNTP、引物、Taq DNA聚合酶等因素对SRAP-PCR扩增结果的影响.确定了me1-em5为扩增中华白海豚基因组DNA的最佳引物对.并进一步确立了适合中华白海豚的SRAP最佳反应体系:25 mm3体系中,模板DNA浓度为0.8 ng/mm3,Mg2+浓度为1.0 mmol/dm3,dNTP浓度为0.1 mmol/dm3,引物浓度为0.2μmol/dm3,Taq DNA聚合酶浓度为0.04 U/mm3.利用该反应体系对10个白海豚样品进行SRAP分析的结果表明,不同样品间DNA谱带清晰、多态性丰富.证实该体系稳定可靠,可以用于中华白海豚的分子标记研究.  相似文献   

厦门至珠江口间沿岸海域中华白海豚分布的调查研究   总被引：1，自引：0，他引：1  

王先艳  妙星  吴福星  闫晨曦  刘文华  祝茜 《台湾海峡》2012,31(2):225-230

厦门海域和珠江口海域是中华白海豚(Sousa chinensis)的2个重要分布区,但对这两地之间沿海白海豚的分布情况还缺乏了解.通过走访厦门至珠江口之间12个渔港码头或渔村的渔民,共获得有效问卷168份,探讨了厦门至珠江口之间沿海历史上和当今中华白海豚的分布情况.结果显示:年龄越大,渔民见过白海豚的比例越高;历史上(20～30a前),东山、汕头、甲子、汕尾和马宫等地沿海曾有较大的中华白海豚种群分布;如今,在东山湾和汕头海域还有中华白海豚的出没.通过进一步的截线抽样法调查,证实了汕头海域还有白海豚的分布,东山湾还有渔民观察到白海豚,但尚待进一步的证实.  相似文献   

不同改良措施对退化羊草（Leymus chinensis）草原的影响     

杨丽娜  宝音陶格涛 《中国沙漠》2008,28(2):312-317

 对1983年通过不同改良措施（播种羊草、耙地、围栏封育）处理过的退化羊草草原群落特征进行比较研究,结果表明：虽然经过23 a的恢复,土壤条件仍有差别,经过耙地处理土壤容重最小,含水量最高;三种改良措施的增产效果都很明显,播种羊草、耙地、围栏封育的群落地上生物量比围栏外分别提高了127.30%、146.64%、138.04%;播种羊草和耙地处理的根茎禾草所占比例较高,而围栏外和围栏封育处理的丛生禾草所占的比例较高;三种改良措施中,旱生植物的绝对生物量都低于围栏外;耙地处理后群落多样性最高,而围栏外均匀性指数最高。综上所述：经过耙地改良所产生的效果维持时间最长,而播种羊草和围栏封育改良的后效依然存在。  相似文献   

中国对虾酚氧化酶的部分生物化学特性的初步研究   总被引：8，自引：0，他引：8       下载免费PDF全文

汪小锋  樊廷俊 《海洋科学》2003,27(4):71-75

以海捕中国对虾(Penacus chinensis)为材料，运用离心、凝胶过滤柱层析和离子交换柱层析的方法从对虾血淋巴中部分纯化出酚氧化酶。实验又以L-DOPAC为底物，分析各种因子对其活力的影响。结果显示酚氧化酶的最适pH值约为6．0，最适温度为40℃，Cu^2 能强烈抑制酚氧化酶的活性，而Mg^2 则能促进其活性，表明酚氧化酶可能是一种金属酶。  相似文献   

3种对虾精子超低温冷冻保存技术研究     

王文琪  杨敬昆  徐世宏  刘清华  李军 《海洋科学》2017,41(9):81-86

作者以日本囊对虾(Marsupenaeus japonicus)、中国明对虾(Fenneropenaeus chinensis)和南美白对虾(Penaeus vannamei)作为实验材料,研究3种稀释液(天然海水,人工无钙海水,Hank’s平衡盐溶液)、3种抗冻剂(二甲基亚砜、丙二醇和丙三醇)、3种稀释比例(5%、10%和15%)、3种不同的降温程序对3种对虾精子超低温冷冻保存效果的影响。冻精解冻后通过台盼蓝染色的方法计算其存活率。结果显示,对于3种对虾,以天然海水作为稀释液,10%PG作为抗冻剂,4℃平衡20 min,以–2℃/min降温至–40℃,–40℃平衡10℃,以–15℃/min降温至–150℃,然后投入液氮作为降温程序进行超低温冷冻保存效果最好,冻精解冻后存活率分别为75.33%±2.56%、69.00%±3.00%和23.33%±8.55%。  相似文献   

Construction of a muscle cDNA library of Chinese shrimp <Emphasis Type="Italic">Fenneropenaeus chinensis</Emphasis> and sequence analysis of the troponin I gene     

Jitao Li  Ping Chen  Jian Li  Ping Liu  Yuying He  Qingyin Wang 《中国海洋大学学报(英文版)》2010,9(1):81-86

A muscle cDNA library of Chinese shrimp (Fenneropenaeus chinensis) was constructed with the SMART™ cDNA Library Construction Kit. The titer of optimal primary library was 7.7×10⁵ pfu mL⁻¹ and that of the amplified library was 3.0×10⁹ pfu mL⁻¹. The percentages of the recombinant clones of primary and amplified libraries were over 98%. The insert sizes were longer than 400 bp with an average of 1000 bp. A positive clone containing a 794 bp insert was sequenced and identified encoding fast skeletal troponin I gene. This library provided a useful resource for the functional genomic research of F. chinensis.  相似文献   

Variations of ion-transport enzyme activities during early development of the shrimps <Emphasis Type="Italic">Fenneropenaeus chinensis</Emphasis> and <Emphasis Type="Italic">Marsupenaeus japonicus</Emphasis>     

Luqing Pan  Li Li  Linjuan Zhang 《中国海洋大学学报(英文版)》2010,9(1):76-80

The ion-transport enzyme activities were studied in the nauplii 1–2, zoea 1–3, mysis 1–3, and postlarva 1–7 of the shrimps Marsupenaeus japonicus and Fenneropenaeus chinensis. The results showed that total ATPase, Na⁺-K⁺-ATPase, and V-ATPase (V-type H⁺-ATPase) activities increased during the early development for both the species, from zero in nauplii to stable levels after zoea stage. The enzyme activities of the latter species were significantly higher than those of the former species after zoea stage (F>F _0.05). The contributions of Na⁺-K⁺-ATPase, V-ATPase and HCO₃ ⁻-ATPase to the total ATPase activity of the two species varied in different developmental stages and accounted for 40%–70%, 22%–46% and 2%–13% in M. japonicus from zoea to postlarva stage, whilst the shares of them were 42%–69%, 28%–44% and 2.5%–22%, respectively in F.chinensis. These findings suggest the possible culture of the two species with varying water quality, especially the salinity and pH.  相似文献