基于正交实验的高含量藻红蛋白紫菜丝状藻体优化调控培养条件研究     

林汝榕  邢炳鹏  柯秀蓉  蔡文旋  林锡煌  张稚兰 《海洋学研究》2014,32(2):67-73

丝状藻体在紫菜养殖的人工苗种生产中非常重要,在利用于分离提取高价值的藻红蛋白方面也具有潜在的利用价值。本研究采集了成熟紫菜叶状体的果孢子释放的初始丝状藻体,基于3因子3水平的正交实验设计方案[采用具有分析交互作用的正交表L₂₇(3¹³)],确定了丝状藻体藻红蛋白含量对环境因子的响应以及优化的调控培养条件。结果表明,温度、光照强度和盐度因子对丝状藻体藻红蛋白含量均有显著影响(P≤0.048 1),并且温度和光照强度或盐度因子间存在交互作用(P ≤0.043 1),但光照强度和盐度因子间的交互作用不显著(P=0.469 8)。获得的优化调控培养条件为:温度18 ℃、光强1 500 lx、盐度20或25。优化调控培养条件下的丝状藻体藻红蛋白含量高达64.73 mg/g.dw(平均值±标准偏差SD为58.24±5.07 mg/g.dw)。  相似文献   

The UV-absorbing compounds in gametophytic blades of Porphyra haitanensis and the effect on formation of sporangial branchlets of conchocelis     

陈烨  严小军 《中国海洋湖沼学报》2007,25(3):343-346

Endogenous levels of UVAC in different parts of matured gametophytic blades of Porphyra haitanensis and the effect of exogenous UVAC on sporangial branchlet formation are studied. The UVAC value in vegetative (36. 78 mg/g) and reproductive (29.25 mg/g) parts of female thallus is higher than their counterparts in male thallus (34.68 mg/g and 20.46 mg/g). The UVAC value of reproductive parts is higher than vegetative parts of same sexual thallus. The optimal UVAC concentration for sporangial branchlet formation is 0.1 mg/g.  相似文献   

EFFECT OF CYTOKININS AND AUXINS ON THE GROWTH OF FREE-LIVING CONCHOCELIS OF PORPHYRA YEZOENSIS     

段德麟  费修绠  任红旭  陈雄  朱莹 《中国海洋湖沼学报》1995,(3)

IAA 3-Indolylaceic acid, NAA a-Naphthylacetic acid and Cytokinins in PESI culture mediumwere used in a study on the effects of plant hormones on the growth of free-living conchocelis ofPorphyra yezoensis which showed that its growth in medium with cytokinins, IAA and NAA was morerapid than that in medium with non-phytohormones, that the optimal concentrations for promotinggrowth were 10μg/L for IAA and ZA (Zeatin), and 0.1 μg/L for BA 6-Benzyl amino purine and KIN6-Furfurylamino- purine. Mix use of NAA, IAA and cytokinins, NAA/ZA 1-1000/1μg/L, NAA/BA10/1-1000μg/L, NAA/KIN 1/1-1000μg/L promoted growth. IAA/ZA 0.1 - 1/0.1 - 1 μg/L, IAA/BA0.1 - 1/0.1 - 10 μg/L IAA/KIN 1/0.1 - 1000μg/L also promoted growth.  相似文献   

SUBUNIT COMPOSITION AND CHROMOPHORE CONTENT OF R-PHYCOERYTHRIN FROM PORPHYRA HAITANENSIS     

高洪峰  纪明侯  曹文达 《中国海洋湖沼学报》1996,(1)

R-phycoerythrin from Porphyra haitanensis exists in two aggregaion states with different molecularweights. A more highly aggregated form, RPE I,Was chromatographed on Bio-Rex 70 column with ureasolution (pH 3.0) as eluent, and the molecular weights of the 3 subunits (α,β,γ) obtained were determinedon SDS-PAGE at 18000, 19200 and 30000, respatively. α subunit carried two phycoerythrobilin (PEB),β subunit, three PEB and one phycourobilin (PUB),γ subunit, one PEB and three PUB chromophores.The molar ratio of α,β, and γ subunits of RPE I wn 6:6:1, and their subunit composition was con-fired to be (αβ)_6γon account of the molecular weight of RPE I, 232000.A lower aggregated form, RPE Ⅱ, contained α and β subunits similar to those of RPE I, but itssubunit composition was the (αβ) monomer of RPE.  相似文献   

坛紫菜磷酸烯醇式丙酮酸羧化酶基因的克隆与序列分析          下载免费PDF全文

张晓娟  王广策  何林文  陈昌生 《海洋科学》2011,35(4):70-76

采用同源克隆的方法和RACE技术,从坛紫菜cDNA中获得了磷酸烯醇式丙酮酸羧化酶(PEPC)的序列,该段序列含2538个核苷酸包含一个终止密码子TAG和191 bp的3'端非编码区(UTR),可编码846个氨基酸.该段序列中无跨膜结构和信号肽序列,推导的氨基酸序列中C末端第813位氨基酸是丙氨酸(A),表明是一个C<,...  相似文献   

有机磷农药对坛紫菜叶状体核酸含量的影响   总被引：3，自引：0，他引：3  

李钦  魏凤琴  陈纪新  王重刚  郑微云 《海洋科学》2004,28(1):20-22

在实验生态条件下研究了不同浓度、不同时间甲胺磷、辛硫磷暴露对坛紫菜(Porphyra haitanensis)核酸(RNA和DNA)含量的影响。结果表明,随着污染时间的延长,所有污染物对RNA、DNA含量的影响基本上表现为先上升后下降的时间效应关系,同一污染物浓度越大就越早出现下降。同时出现随暴露剂量的增加,RNA、DNA含量先上升后下降或全部下降的剂量～效应关系。  相似文献