大型海藻中环二肽类抑藻活性化合物的分离纯化     

庄丽雯  周文静  毛奕淋  郭赣林  王长海  孙颖颖 《海洋通报》2022,(6)

采用茚三酮试剂显色的薄层原位化学反应和抑藻活性检测方法,从龙须菜、条斑紫菜、鹿角藻和裙带菜中筛选环肽,发现龙须菜和条斑紫菜中存在具有抑藻活性的环肽。以龙须菜和条斑紫菜粉末为原料,采用硅胶柱层析和（或）硅胶薄层层析制备等方法,从龙须菜中分离到环（脯氨酸-甘氨酸）、环（丝氨酸-脯氨酸）和环（丙氨酸-色氨酸）,从条斑紫菜中纯化到环（亮氨酸-脯氨酸）和环（缬氨酸-酪氨酸）。除环（丝氨酸-脯氨酸）外,其余4种环二肽均是首次从相应大型海藻中获得。环（脯氨酸-甘氨酸）、环（亮氨酸-脯氨酸）和环（缬氨酸-酪氨酸）明显抑制了强壮前沟藻、赤潮异弯藻和球形棕囊藻的生长,对米氏凯伦藻和中肋骨条藻有选择性抑制作用。在50 μg/mL时,3种环二肽对强壮前沟藻、赤潮异弯藻和球形棕囊藻的生长抑制率≥50%（第4d）;环（脯氨酸-甘氨酸）对米氏凯伦藻以及环（亮氨酸-脯氨酸）对中肋骨条藻的生长抑制率超过或接近50%。随后,获得环二肽对赤潮微藻生长的半抑制效应浓度EC50-96h。结果表明,环（脯氨酸-甘氨酸）、环（亮氨酸-脯氨酸）和环（缬氨酸-酪氨酸）在抑制米氏凯伦藻、球形棕囊藻或赤潮异弯藻方面比重铬酸钾更有优势,有望开发为环境友好型赤潮微藻抑藻剂。  相似文献   

Exploring valid internal-control genes in Porphyra yezoensis （Bangiaceae） during stress response conditions     

王文磊  吴晓洁  王超  贾兆君  何林文  韦一凡  牛建峰  王广策 《中国海洋湖沼学报》2014,32(4):783-791

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条斑紫菜低覆盖度基因组草图分析     

牛建峰  高胜寒  骆迎峰  袁野  王广策  胡松年 《海洋科学集刊》2011,50(6):76-81

对条斑紫菜(Porphyra yezoensis)进行Solexa 高通量测序, 获得低覆盖度全基因组草图。该基因组草图大小约220 Mbp, GC 质量分数53.08%; 包含26 629 个预测基因, 其中16 409 个基因具有内含子,平均每个基因含2.22 个内含子; 基因结构分析表明具有内含子的基因平均长度为2 214 bp, 内含子平均大小319 bp; 代谢通路分析表明嘌呤代谢是含有蛋白数量最多的代谢途径。本研究所得条斑紫菜低覆盖度全基因组草图快速获取了基因组大小和蛋白编码基因结构等基本信息, 证明了使用Solexa 高通量测序技术对条斑紫菜进行全基因组测序的可行性。  相似文献   

Subunit composition and chromophore content of R-phycoerythrin fromPorphyra haitanensis     

Gao Hong-feng  Ji Ming-hou  Cao Wen-da 《中国海洋湖沼学报》1996,14(1):36-40

R-phycoerythrin fromPorphyra haitanensis exists in two aggregation states with different molecular weights. A more highly aggregated form, RPE I, was chromatographed on Bio-Rex 70 column with urea solution (pH 3.0) as eluent, and the molecular weights of the 3 subunits (α, β, γ) obtained were determined on SDS-PAGE at 18000, 19200 and 30000, respectively. α subunit carried two phycoerythrobilin (PEB); β subunit, three PEB and one phycourobilin (PUB); γ subunit, one PEB and three PUB chromophores. The molar ratio of α, β, and γ subunits of RPE I was 6: 6: 1, and their subunit composition was confired to be (αβ)₆γ on account of the molecular weight of RPE I, 232000. A lower aggregated form, RPE II, contained α and β subunits similar to those of RPE I, but its subunit composition was the (αβ) monomer of RPE. Contribution No. 2108 from the Institute of Oceanology, Chinese Academy of Sciences  相似文献   

Construction of Porphyra yezoensis Pure Line from Protoplasts and Its 18S rDNA Sequence Determination     

LIU Hongquan )  )  YU Wengong )  *  DAI Jixun )  GONG Qianhong )  SHI Xiaochong )  YANG Kunfeng ) ) Institute of Marine Drugs  Foods  Ocean University of China  Qingdao  P..R.China ) College of Marine Life Sciences  Ocean University of China  Qingdao  P..R.China 《中国海洋大学学报(英文版)》2004,3(1)

1　Introduction　PorphyraisthemainobjectforalgafarmingandplaysaveryimportantroleinChinesemarineindus tries.Recently ,therehasbeenagreatlossinPor phyracultivationduetothedegenerationoftheculti var,sothereisanincreasingdemandforgoodPor phyracultivars .Theprerequisiteofthetraditionalbreed ingandbioengineeringresearchofPorphyraistheconstructionofpurelines .Traditionally ,theclassifi cationofPorphyrawasaccordingtotheirmorphologi calcharacteristics .However ,mostmorphologicalfea turesofPorphyraar…  相似文献   

Construction of Porphyra yezoensis Pure Line from Protoplasts and Its 18S rDNA Sequence Determination     

LIUHongquan YUWengong DAIJixun GONGQianhong SHIXiaochong YANGKunfeng 《中国海洋大学学报(英文版)》2004,3(1):60-64

The wild Porphyra yezoensis collected from the Qingdao coast was used to prepare protoplasts by enzyme digestion. The pure line was constructed by cultivating the protoplasts. The 18S rDNA of the P. yezoensis pure line was cloned and sequenced. Sequence analysis was executed for this sequence and other 22 sequences retrieved from GenBank. A phylogenetic tree was constructed using the neighbor-joining method. The results revealed a high diversity of 18S rDNA sequences in genus Porphyra and the considerable variation of 18S rDNA sequences in different strains of the same species P. yezoensis and P. tenera. Significant difference of 18S rDNA sequence was observed between P. yezoensis from Qingdao, China, and the two strains of P. yezoensis from Japan, but the three strains of P. yezoensis formed a stable clade in the phylogenetic tree. These results indicate the possibility of interspecies and intraspecies discrimination of Porphyra using the 18S rDNA sequences.  相似文献   

Ultrastructure of Single Cells, Callus-like and Monospore-like Cells in Porphyra yezoensis Ueda on Semi-solid Culture Medium     

梅俊学  沈颂东  姜明  费修绠 《中国海洋湖沼学报》2003,(2)

ＩＮＴＲＯＤＵＣＴＩＯＮＴｈｅｓｉｎｇｌｅｃｅｌｌｓａｎｄｐｒｏｔｏｐｌａｓｔｓｏｆＰｏｒｐｈｙｒａｇａｍｅｔｏｐｈｙｔｅｂｌａｄｅｓｃｏｕｌｄｄｅｖｅｌｏｐｉｎｔｏｐｌａｎｔｌｅｔｓｄｉ ｒｅｃｔｌｙｏｒｉｎｔｏｃａｌｌｕｓ ｌｉｋｅｃｅｌｌｓ (ＺｈａｏａｎｄＺｈａｎｇ ,1 981 ;Ｐｏｌｎｅ Ｆｕｌｌｅｒｅｔａｌ.,1 984 ;Ｃｈｅｎ ,1 989;ＷａｎｇａｎｄＹａｎ ,1 990 ;Ｇａｌｌｅｔａｌ.,1 993;Ｃｈｅｎ ,1 997;ＭｅｉａｎｄＦｅｉ,2 0 0 1 ) ,ａｎｄｔｈｅｉｒｇｒｏｗｔｈａｎｄｄｅｖｅｌｏｐｍｅｎｔｕｎｄｅｒ…  相似文献   

Efficient gusA transient expression in Porphyra yezoensis protoplasts mediated by endogenous beta-tubulin flanking sequences     

Gong Qianhong  Yu Wengong  Dai Jixun  Liu Hongquan  Xu Rifu  Guan Huashi  Pan Kehou 《中国海洋大学学报(英文版)》2007,6(1):21-25

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Study on Cryopreservation of Porphyra yezoensis Conchocelis     

ZHOU Wenjun LI Yun DAI Jixun 《中国海洋大学学报(英文版)》2007,6(3):299-302

Cryopreservation of Porphyra yezoensis conchocelis was conducted with cryoprotectants and a proposed pretreatment procedure and thawing methods explored. Six cryoprotectants combined by DMSO with ethylene glycol （EG）, propylene glycol （PEG）, sorbitol and sucrose were developed. The effect of prefreezing at -40℃ or -20℃ for different time durations was compared and the thawing methods were screened. It was shown that the cryoprotectant including 10% DMSO with 0.5 molL^-1 sorbitol exhibited the optimal effect. The ideal pretreatment was that conchocelis segments were stayed for 20min at -40℃ before stored in liquid nitrogen, and 40℃ water bath was proper for quick thawing. The highest recovery rate of cryopreserved P. yezoensis conchocelis reached 89.41%.  相似文献   

Comparative studies on structural feature of agar polysaccharides fromPorphyra haitanensis grown in south and north China     

Gao Hongfeng  Ji Minghou  Cao Wenda 《中国海洋湖沼学报》1993,11(1):25-33

The structural feature of agar polysaccharides fromPorphyra haitanensis grown in south China and transplanted to the north was investigated by fractionation on DEAE—Sephadex A 50, chemical analysis, and infrared and¹³C-NMR spectroscopy. The agars composed mainly of charged molecules were eluted from DEAE—Sephadex A 50 with 1.0 mol/L NaCl solution from the southernP. haitanensis and with 0.5 mol/L NaCl from the northern one. The¹³C-NMR spectra showed that agarobiose [(1→3)-β-D-galactopyranosyl-(1→4)-3,6-anhydro-α-L-galactopyranose] and the biological precursor of agarobiose [(1→3)-β-D-galactopyra nosyl-(1→4)-6-sulfate-α-L-galactopyranose] were the major disaccharide repeating units in the charged fractions. The content of the biological precursor in the agar polysaccharides from southernP. haitanensis was higher than that in the northern one, the content of the biological precursor extracted from cold water was higher than that from hot water, and the content of 6-OMe-D-galactose in the southernP. haitanensis polysaccharides was higher than in the northern one. This distinct difference will be of significance for further study of the physiological characters ofP. haitanensis. Contribution No. 1849 from the Institute of Oceanology, Academia Sinica.  相似文献