2005年春初浙江近海的低温特征及其对大规模东海原甲藻赤潮发生的影响   总被引：2，自引：0，他引：2  

朱德弟  陆斗定  王云峰  苏纪兰 《海洋学报》2009,31(6):31-39

在长江口附近和浙江近海海域,近些年来每年春季几乎都发生大规模的东海原甲藻(Prorocentrum donghaiense)赤潮,时间通常是在4月底或5月初,但2005年却一直到5月底才发生。本文根据近几年在浙江近海进行的多个春季航次的调查资料和有关站位的气象资料,分析了该海区2005年春初的温度特征及其对大规模东海原甲藻赤潮发生的影响,阐明了2005年春季大规模东海原甲藻赤潮迟发的主要物理环境原因。结果表明,2005年3月浙江近海水温比多年同期平均值偏低,且这种情况在近岸区更为明显;与2004年同期相比,2005年春初(4月初)浙江近海水温偏低,2005年和2004年的这种水温差异在近岸和中上层的低盐水里最为明显,而在远岸和下层的高盐水水温差异则相对要小;近岸低盐水最低温度2005年比2004年低约2.6℃;2005年1—3月浙江近海特殊的气象条件(10 a中最低的气温和强劲的偏北大风)和2004/2005年冬季较弱的台湾暖流是形成4月初浙江近海低温特征的主要原因;2004年冬季和2005年春初浙江近水温偏低很可能是2005年春季东海大规模原甲藻赤潮迟发的主要原因。  相似文献   

Exposure to Prorocentrum minimum Induces Oxidative Stress and Apoptosis in the Ridgetail White Prawn,Exopalaemon carinicauda     

MU Cuimin  GE Qianqian  LI Jian 《中国海洋大学学报(英文版)》2019,(3):727-734

Prorocentrum minimum is a bloom-forming, planktonic mixotrophic dinoflagellate, and can cause stress in shrimp ponds. In this study, healthy Exopalaemon carinicauda were exposed to 5 × 10^4 cells mL^-1 P. minimum for 72 hours to investigate the adverse effect of P. minimum on shrimps. Elevated superoxide dismutase(SOD) activity and malondialdehyde(MDA) content, reduced total antioxidant capacity(T-AOC) and catalase(CAT) activity, and regulatory glutathione peroxidase(GPX) activity were found in the hemolymph of E. carinicauda after exposure to P. minimum. In this study, P. minimum exposure induced oxidative stress and caused significant oxidative damage to E. carinicauda. P. minimum exposure increased the expression of HSP70 gene in the hemocyte, gills and hepatopancreas. Compared with the enhanced level of caspase-3 gene mR NA in the hemocyte and gills, the up-regulation of caspase-3 gene in the hepatopancreas was only observed from 3 to 6 h, and then the mRNA level of glutathione-S-transferase(GST) gene increased. These results indicated that GST might be involved in the shrimp hepatopancreas’ defense against P. minimum exposure. The present study demonstrates that exposure to P. minimum could induce oxidative stress and apoptosis in E. carinicauda. The SOD activity, HSP70 and GST(in the hepatopancreas) were evoked to protect cells from oxidative stress and apoptosis. This study will provide new insights into the toxic mechanism of P. minimum on shrimps.  相似文献   

不同状态浒苔对东海原甲藻生长的影响     

林锟  杨雪  谭丽菊  王江涛 《海洋学报》2020,42(8):69-75

近年来富营养化等海洋污染现象日益加重,我国近海每年都会暴发浒苔绿潮和微藻赤潮现象。本文在实验室模拟条件下,研究了不同状态不同含量的浒苔（Ulva prolifera）,即浒苔新鲜藻体、浒苔培养液和浒苔干粉末与东海原甲藻（Prorocentrum donghaiense）共培养时产生的竞争抑制作用。结果表明,浒苔新鲜藻体、浒苔培养液和浒苔干粉末在初始质量含量大于1 g/L的条件下都会对东海原甲藻产生抑制作用,浒苔干粉末的抑制强度远远超过浒苔培养滤液和浒苔新鲜藻体。在二者共培养时,出现了“低促高抑”的现象,即浒苔含量较低时会促进东海原甲藻的生长,而高含量的浒苔则抑制东海原甲藻的生长,且抑制作用随浒苔含量的升高而增大。浒苔对东海原甲藻的这种抑制作用,一方面可能来自于营养物质的竞争和消耗,另一方面可能来自于浒苔释放的化感物质对东海原甲藻产生的影响。利用浒苔对东海原甲藻的抑制作用,可为东海原甲藻赤潮的生物防治提供一种思路与方法。  相似文献   

Blooms of Prorocentrum donghaiense reduced the species diversity of dinoflagellate community     

王欢  胡章喜  柴召阳  邓蕴彦  詹子锋  唐赢中 《海洋学报(英文版)》2020,39(4):110-119

Most of reported harmful algal blooms(HABs)of microalgae(75%)have been caused by dinoflagellates.Studies on the negative effects of HABs have generally focused on animals,valuable organisms in particular,and environmental factors such as dissolved oxygen and nutrients,but relatively fewer on community level,particularly that using metagenomic approach.In this study,we reported an investigation on the effects of a HAB caused by the dinoflagellate Prorocentrum donghaiense on the species diversity and community structure of the dinoflagellate sub-community via a pyrosequencing approach for the samples taken before,during,and after the bloom season of P.donghaiense in the East China Sea.We sequenced partial 28S rRNA gene of dinoflagellates for the field samples and evaluated the species richness and diversity indices of the dinoflagellate community,as a sub-community of the total phytoplankton.We obtained 800185 valid sequences(categorized into 560 operational taxonomic units,OTUs)of dinoflagellates from 50 samples and found that the biodiversity of dinoflagellate community was significantly reduced during the blooming period in comparison to that in pre-and after-blooming periods,as reflected in the four diversity indices:the species richness expressed as the number of OTUs,Chao1 index,Shannon index(evenness),and Gini-Simpson index.These four indices were all found to be negatively correlated to the cell density of the bloom species P.donghaiense.Correlation analyses also revealed that the P.donghaiense cell abundance was correlated negatively with NO3--N,and NO2--N,but positively with total nitrogen(TN)and total phosphorus(TP).Principal coordinates analysis(PCoA)showed that the community structure of dinoflagellates was markedly different among the different sampling periods,while the redundancy analysis(RDA)revealed P.donghaiense abundance,salinity,NO3--N,and SiO32-were the most four significant factors shaping the dinoflagellate community structure.Our results together demonstrated that HABs caused by the dinoflagellate P.donghaiense could strongly impact the aquatic ecosystem on the sub-community level which the blooming species belongs to.  相似文献   

基于LAMP-LFD技术的有害赤潮藻东海原甲藻(Prorocentrum donghaiense)快检方法     

邹怡欣  黄海龙  乔龙亮  党晨阳  庞建虎  吕佩文  王佳棋  朱鹏 《海洋与湖沼》2018,49(3):586-593

以东海原甲藻的ITS序列(Internal transcribed spacer,ITS)为检测靶标,将生物素标记的环介导恒温扩增技术(Loop-mediated isothermal amplification,LAMP)扩增产物与经异硫氰酸荧光素(Fluorescein isothiocyanate,FITC)标记的探针特异性杂交,并结合横向流动试纸条(Lateral flow dipstick,LFD)肉眼直接观察检测结果,建立了有害赤潮藻东海原甲藻(Prorocentrum donghaiense)的快速检测技术。经优化后的最适条件为63°C、30min,较常规PCR扩增缩短约2h。结果表明:LAMPLFD可特异性检出东海原甲藻,对常见赤潮藻检测结果均为阴性;其对东海原甲藻基因组DNA的检测最低限为47pg/μL,是常规PCR技术(以F3/B3为引物)的10倍。LAMP-LFD技术能高效、特异地检出东海原甲藻,仪器设备依赖性低,结果可视化,有望成为赤潮原因种检测监控的常规方法。  相似文献   

烟台四十里湾一次血红哈卡藻赤潮过程的分析   总被引：2，自引：0，他引：2  

喻龙  郝彦菊 《海洋科学进展》2009,27(4)

根据2007年8月烟台四十里湾海域血红哈卡藻(Akashiwo sanguinea)赤潮的监测数据,对赤潮发生时及发生前、后的水文气象、化学、生物因子进行了分析.结果表明,8月份的持续降雨使大量的营养盐从陆源输入,使该海区海水呈极度富营养化状态,引起赤潮的爆发.赤潮发生前及赤潮消退之后,该海区营养盐浓度均处于较低水平.  相似文献   

Identification of Prorocentrum minimum and Takayama pulchella by fluorescence in situ hybridization through epifluorescence microscopy and flow cytometry     

HOU Jianjun          LAI Hongyan  HUANG Bangqin  CHEN Jixin College of Fisheries  Huazhong Agricultural University  Wuhan  China College of Life Sciences  Hubei Normal University  Huangshi  China State Key Laboratory of Marine Environmental Science  Environmental Science Research Center  Xiamen University  Xiamen  China Key Laboratory of Freshwater Fish Germplasm Resources  Biotechnology  Yangtse River Fisheries Research Institute  Jinzhou  China State Key La... 《海洋学报(英文版)》2009,28(2)

Partial rDNA sequences of Prorocentrum minimum and Takayama pulchella were amplified, cloned and sequenced, and these sequence data were deposited in the GenBank. Eight oligonucleotide probes (DNA probes) were designed based on the sequence analysis. The probes were employed to detect and identify P. minimum and T. pulchella in unialgal and mixed algal samples with a fluorescence in situ hybridization method using flow cytometry. Epifluorescence micrographs showed that these specific probes labeled with flu...  相似文献   

运用PCR-DGGE技术对3种原甲藻18S rDNA部分序列的差异性分析     

孙静  于志刚  甄毓  张涛  赵丽媛  袁健  米铁柱 《中国海洋大学学报(自然科学版)》2010,40(2)

采用3对特异性引物,对中国近海常见3种原甲藻的18S rDNA的部分序列进行PCR扩增,运用变性梯度凝胶电泳技术(Denaturing Gradient Gel Electrophoresis,DGGE)对PCR产物进行了差异性分析。结果表明,各对引物具有不同的区分效果,选择包含较多差异碱基的短片段进行扩增,可以对藻种进行更好的区分;PCR-DGGE技术具有较高的灵敏性,不同藻种间即使1个碱基的差异也可以区分开;PCR-DGGE技术可以对3种原甲藻进行区分。  相似文献   

东海原甲藻吸收光谱特性分析     

周虹丽  朱建华  杨安安  简伟军  林少君 《海洋技术学报》2011,30(1):65-67,77

以东海原甲藻为研究对象,通过室内和室外的一系列培养实验.通过对不同生长时期其光吸收特性的变化和不同光强对其光吸收特性的影响进行研究,分析东海原甲藻先吸收特性研究及其爆发的可能机制.结果表明在培养的不同时期东海原甲藻光吸收特性差异明显,在不同光强条件下,当遮光率为77.7%时,最适于东海原甲藻生长.  相似文献   

Acute Toxicity of Formalin to the Red-tide Dinoflageilate Prorocentrum minimum Schiller （Protozoa, Mastigophora）     

XUHenglong SONGWeibo ZHUMingzhuang QIUYantao MAHonggang 《中国海洋大学学报(英文版)》2005,4(1):89-92

The acute toxic levels of formalin to a marine red-tide dinoflagellate Prorocentrum minimum Schiller (Mastigophora, Dinoflagellida) were determined by linear regression analysis. The data obtained revealed that there is a close regression relationship between logarithmic concentrations of formalin and mortality probit scales of the organism when exposure times are 1, 2, 6, 12, 24 h. The median lethal concentrations (LC50 values) obtained from correlation analysis for these exposure times (with 95% confidence intervals), were 11.83, 6.76, 4.37, 4.27 and 3.98mgL^-1 respectively. A toxicity curve was obtained to connect the exposure time and LC50 value from the correlation between them. The results indicated that P. minimum could be killed or fixed by formalin in the concentration range of 4-12mgL^-1 within 1-24h.  相似文献