Effects of food concentration and diet on chromophoric dissolved organic matter accumulation and fluorescent composition during grazing experiments with the copepod Calanus finmarchicus     

Urban-Rich  Juanita; McCarty  James T; Shailer  Mark 《ICES Journal of Marine Science》2004,61(4):542-551

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中国对虾血淋巴蛋白质、葡萄糖含量的研究   总被引：7，自引：0，他引：7  

罗日祥 《海洋与湖沼》1996,27(5):476-480

于１９９２－１９９３年５月和１０月，在胶州湾捕捞中国对虾亲虾，９月在莱州和胶南采集的人工养殖中国对虾，采用双缩脲法测定其血淋巴中的血清总蛋白含量，用０－ＴＢ法测定血清葡萄糖含量，并研究注射灭菌海水、海洋弧菌后，对中国对虾血清总蛋白含量和血清葡萄糖含量的影响规律。  相似文献   

Variations of backscatter strength along the super slow-spreading Southwest Indian Ridge between 57°E and 70°E     

Daniel Sauter  Vronique Mendel 《Marine Geology》1997,140(3-4):237-248

Simrad EM12 backscatter strength data of the Southwest Indian Ridge (SWIR), between 57°E and 70°E, are used to reveal the along-axis segmentation of this super slow-spreading ridge. The backscatter properties of different geologic domains, like bathymetric highs and oblique basins within the rift valley, are characterized using 66 small test sites. We show that backscatter strength is higher on bathymetric swells, corresponding to segment centres, and lower in deep oblique basins corresponding to axial non-transform discontinuities and fracture zones. This contrast between segment centres and discontinuities is produced by both a thicker sediment cover and less frequent volcanic eruptions at segment ends. Using the model of Mitchell (1993), sediments have been estimated to be 2 to 5 m thicker in these areas than at segment centres. The distribution of the seamounts within the rift valley is controlling the long-wavelength variations of the mean backscatter strength calculated along the axis. Lower densities of seamounts and thicker sediments are producing lower and heterogeneous reflectivity levels in the deepest part of the axial valley floor between 61°45′E and 63°45′E. We propose that cooler mantle temperatures inducing construction of fewer volcanoes occur beneath this part of the ridge. The mean backscatter strength along the SWIR axis decreases dramatically toward the Rodrigues Triple Junction suggesting that volcanic production is reduced between 68°20′E and 69°20′E and that the transition from amagmatic tectonic deformation at the triple junction to new seafloor spreading occurs between 69°20′E and 70°E.  相似文献   

Sediment trapping and bypassing characteristics of a stable tidal inlet at Kaohsiung Harbor, Taiwan   总被引：2，自引：0，他引：2  

James T. Liu  Li-hua Hou 《Marine Geology》1997,140(3-4):367-390

Around the artificially stabilized tidal inlet that connects Kaohsiung Harbor to Taiwan Straight, 203 surficial samples of the sea floor were taken from the nearshore, in the outer harbor, and portions of the inner harbor. The bathymetry of the same area was also surveyed. The sand fraction in each sample was analyzed for the grain-size composition with a custom-built rapid sediment analyzer. A total of twenty-one size-classes were used in the analysis. Three hypothetical sediment sources were assumed to have influenced the spatial grain-size patterns in the study area: the northward and southward littoral drifts, and the sediments exported from the harbor. After reducing the influence of each hypothetical sediment source separately, the data were analyzed using empirical orthogonal (eigen) function (EOF) analysis. The results indicate that the northward long-term littoral drift is the dominant direction of sediment transport in the nearshore of the study area. A conceptual model for four different sediment trapping and bypassing patterns are proposed. (1) Excess bypass (or net outflux): this bypass pattern is characterized by the export of fine-grained sediments (mud and size classes in the very fine sand fraction) from the interior of the harbor. (2) Partial bypass (or partial trapping): this bypass pattern is represented by the size-classes in fine sand fraction. The amount of these grain sizes entering the outer harbor through the inlet is more than the amount exiting at the inlet mouth, resulting in the retention of a portion of these grain sizes. (3) Total bypass (or zero trapping): grain sizes that exhibit this bypass pattern do not come near the mouth of the inlet. These grain sizes include medium and coarse sand fractions. (4) Lag deposits: this group includes the size classes in the very coarse sand fraction, which are largely concentrated in the scour pit immediately seaward of the inlet mouth. In general, surficial sediment grain-size patterns represent a time-averaged response of the substrate to the transport processes over the time scale of at least two seasons. The differential associations of grain-size groups with various topographic features in the study area suggest morpho-textural relationships exist between the sea floor topography and grain size distribution patterns.  相似文献   

从鳕鱼碎肉中提取水解蛋白   总被引：15，自引：0，他引：15  

王长云  魏玉西 《海洋湖沼通报》1995,(4):33-39

用蛋白酶水解法从鳕鱼加工废弃物－鳕鱼碎肉中提取水解蛋白。鳕鱼碎肉经枯草杆菌ＡＳ１．３９８中性蛋白酶２４０ＩＵ／ｍｌ，ｐＨ７．０，５０℃下水解２ｈ；再经黄曲霉ＩＦＦＩ２３４２（蛋白酶活力２０００ＩＵ／ｇ）１．５％（ｗ／ｖ），５０℃，ｐＨ６．０下水解６ｈ以脱苦味。水解液经真空浓缩后喷雾干燥得水解蛋白粉。该制品易溶于水，无苦味，有海鲜味。其氨基酸组成合理，是优良的蛋白质制品。  相似文献   

The Effects of Zn, Mn, and Co Accumulation on Growth and Chemical Composition of Fucus vesiculosus L under Different Temperature and Salinity Conditions     

IVKA M. MUNDA  V. HUDNIK 《Marine Ecology》1988,9(3):213-225

Abstract. Combined effects of temperature, salinity, and metal enrichment (Zn, Mn, Co) on the growth rate and chemical composition of Fucus vesiculosus L. were studied in culture. Factorial combinations of two temperatures and three salinities were used. The results indicated that the accumulation of Zn and Mn into algal tissue was enhanced by dilution of the media, whereas Co accumulation exhibited maxima at the medium salinity. Zn accumulation was enhanced by temperature, while the opposite was true for Mn and Co.
In the presence of metals the usual dilution-induced changes in growth activity and chemical composition of Fucus plants were altered, while responses to the temperature factor remained unchanged. In diluted media, growth activity and mannitol content decreased along with a simultaneous increase of protein content. In the presence of Zn and Mn the mannitol content exhibited peaks at the medium salinity; in the presence of Co the dilution-induced pattern remained unchanged. Salinity-induced changes in growth and protein content were altered in the presence of metals. Maxima in growth rates were found at the medium salinity. Growth was higher in extremely diluted media than in scawater, indicating an inversion of responses to the salinity factor. Both the growth rate and mannitol content were lowest under the influence of Zn.  相似文献   

Characteristics and phylogeny of light-harvesting complex gene encoded proteins from marine red alga Griffithsia japonica     

LIU Chenlin  HUANG Xiaohang  LEE Yookyung  LEE Hongkum  LI Guangyou 《海洋学报(英文版)》2005,24(2):120-130

1 Introduction T he light-harvesting com plex (LH C ) com posedofphotosynthesis pigm ents and associated proteins arelocated on the thylakoid m em brane of chloroplast inhigherplants and algae. T he capture oflightenergy isthe first step in photosynthesis and is largely accom -plished by the pigm entprotein com plexes. To m axi-m ize the capture of light energy under various envi-ronm entalconditions, the LH C s w ith differentchro-m ophore com positions have evolved in differentpho-tosynth…  相似文献   

重组斑马鱼Gli2蛋白N端肽段的表达及纯化     

谭训刚  张培军  杜少军 《海洋科学集刊》2006,47(47):140-148

Hedgehog(Hh)信号及其信号传递系统在生物机体发育中起着非常重要的作用。从Hhs分泌到其靶基因收到来自Hhs的信号的过程中，包括很多步骤，而每一步骤都有不同的因子参与作用。在其传递的过程中，参与受体细胞将胞外的信号传到胞内调节其目标基因的一个重要因子是果蝇分节-极性基因cubitus in terruptus(Ci)及其脊椎动物同源基 因-G li编码的蛋白。  相似文献   

重组七鳃鳗PHB2蛋白的原核表达及纯化     

李铁松  王颖  高杨  李庆伟 《海洋科学》2015,39(4):37-42

为了研究PHB2蛋白的生物学活性及后续抗体的制备,作者以前期构建的p MD18-T-Lm-PHB2质粒为模板,PCR扩增Lm-PHB2基因全长CDS区,PCR产物经Hind III和Eco R I双酶切后连接至表达载体p ET-32a,构建重组质粒p ET-32a-Lm-PHB2。将鉴定正确的重组质粒转化入大肠杆菌(Escherichia coli)Rosetta Blue,经IPTG诱导表达后,表达产物通过SDS-PAGE和Western blotting进行检测,利用镍柱亲和层析纯化得到纯度较高的目的蛋白r Lm-PHB2。结果表明,经PCR、双酶切和测序鉴定,所构建的重组质粒p ET-32a-Lm-PHB2序列正确,表达产物存在于裂解菌液的上清和沉淀中,经SDS-PAGE和Western blotting证实在约47 ku处有目的蛋白r Lm-PHB2的表达条带。本研究构建了重组七鳃鳗(Lampetra japonica)PHB2蛋白的原核表达载体,并大量表达和纯化目的蛋白,为该蛋白后续的抗体制备及生物活性研究奠定了基础。  相似文献   

长牡蛎nacrein 基因的克隆、结构及进化分析     

宋小瑞  王晓通  李莉  张国范 《海洋科学》2015,39(10):85-93

首次在长牡蛎(Crassostrea gigas)中克隆得到一种新的贝壳基质蛋白nacrein-like protein F3的全长c DNA序列。nacrein-like protein F3基因c DNA全长1499bp,其中编码区长度为1242bp,编码一条含413个氨基酸残基的多肽链。氨基酸序列比对和结构域分析均表明其为合浦珠母贝(Pinctada fucata)nacrein的同源蛋白,含有1个保守的α-碳酸酐酶结构域,但由于重复结构域的插入,α-碳酸酐酶结构域被间隔成2个亚结构域。系统进化分析显示nacrein-like protein F3与贻贝(Mytilus californianus)nacrein-like protein进化关系最近。此外,在软体动物中,双壳纲nacrein-like proteins进化速度相对较快,推测与寒武纪时期剧烈的环境变化有关,如影响贝壳形成的海水化学变化。  相似文献