The selection of alkaline protease-producing yeasts from marine environments and evaluation of their bioactive peptide production     

LI Jing  CHI Zhenming  WANG Xianghong  PENG Ying  CHI Zhe 《中国海洋湖沼学报》2009,27(4)

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用于构建生物系统发育树的古DNA序列     

唐先华  赖旭龙 《地质科技情报》2001,20(3):35-40

主要综述了古 DNA研究进展以及结合古 DNA序列和基因库中的数据构建系统发育树。这些研究能解决许多有关演化生物学、考古学、人类演化及迁移、动植物的家养和驯化过程及早期农业的发展、考古点的动植物残骸的精确鉴定及地质演化等问题。另外 ,还列举了一些重要的实例  相似文献   

18S rRNA基因在节肢动物系统进化研究中的意义   总被引：7，自引：0，他引：7  

吴平  张克云  杨群 《现代地质》2000,14(3):255-362

采用多种分析方法对 4 0种节肢动物的 1 8Sr RNA基因全序列进行了分子系统学研究 ,结果表明 ,现生 4个类群的系统发生关系为 ( (多足类 +鳌肢类 ) (昆虫类 +甲壳类 ) ) ,且表现为辐射状 (爆发式 )分支形式 ;甲壳类的各个类群间也呈爆发式进化 ;介形类的速足介和丽足介两个类群是非单系起源的 ,它们的分化发生在甲壳纲各类群的分化之前 ;所有分析均显示速足介类支系中的金星介科的文杜维异壳介 ( H eterocypris vandouwei)、中华薄壳介 ( Dolerocyprissinensis)、Stenocyprismajor和 H eterocyprissp.组成一个单系类群。  相似文献   

一株分离微拟球藻LAMB0002的鉴定     

梁鑫  朱葆华  杨官品  潘克厚 《中国海洋大学学报(自然科学版)》2014,44(7):39-45

微藻的分类鉴定是进一步应用的前提。微拟球藻属的6个种都是单细胞微藻,形态简单,难以用形态学方法进行准确鉴定。本研究分离了一株微拟球藻,结合光学显微镜和透射显微镜观察、脂肪酸和色素组成分析、18SrRNA基因和rbcL基因序列分析,将该藻株鉴定为Nannochloropsis oceanica LAMB0002。本研究进行了微藻分类系统的初步探索,为今后形成系统的微藻分类体系奠定了基础。  相似文献   

Morphology and Molecular Identification of Ulva Forming Green Tides in Qingdao, China     

KONG Fanna  MAO Yunxiang  CUI Fujun  ZHANG Xingkui  GAO Zhen 《青岛海洋大学学报(英文版)》2011,(1):73-79

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Morphology and molecular identification of Ulva forming green tides in Qingdao, China     

Fanna Kong  Yunxiang Mao  Fujun Cui  Xingkui Zhang  Zhen Gao 《中国海洋大学学报(英文版)》2011,10(1):73-79

Green tides are caused by the proliferation of chlorophytes under suitable hydrographic conditions.These blooms lead to environmental degradation and negatively impact the waters and seagrass beds,as well as fishing and other recreational activities in the bay.A comprehensive ecological understanding of the bloom dynamics,including the origin and persistence,is needed to foster management decisions.The algae in the great majority of green tide blooms usually belong to two genera of Ulvophyceae,Ulva and Enteromorpha.Ulva has been observed more often in recent years.In China,green tides occurred for the first time in the middle area of the Yellow Sea in 2007,and a large-scale algae blooming broke out in the middle and southern areas of the Yellow Sea in late May 2008.We identified them as Ulva prolifera by comparative analysis of the rDNA internal transcribed spacer 1 (ITS1),5.8S and ITS2 sequences in combination with microscopic observation.Morphological differences were found between the free-floating algae and the attached thalli.Various reproduction patterns of the free-floating algae include sexual,asexual and vegetative propagations,which played important roles in the long-term green tide persistence in China.The ITS sequences of the blooming algae were identical to those of the samples from the Lianyungang sea area but were different from the attached samples from the Qingdao sea area.The results infer that the blooms are originated from other sea areas rather than from the local attached populations.  相似文献   

Antimicrobial activities of novel cultivable bacteria isolated from marine sponge Tedania anhelans     

曾臻  赵晶  柯才焕  王德祥 《中国海洋湖沼学报》2013,31(3):581-590

Marine sponge Tedania anhelans distributes throughout the intertidal zone of Fujian, southeastern China, and is a potential source of natural bioactive products. The sponge harbors a large number of bacterial groups that have been identified using various techniques, including fluorescent in situ hybridization (FISH). Fractionation of dissociated sponge allowed isolation of 25 bacterial species. Based on 16S rRNA gene sequencing, phylogenetic analysis attributed most of these eubacteria to α-Proteobacteria, γ-Proteobacteria, Cytophaga/Flavobacterium/Bacteroidetes (CFB group), and the family Bacillaceae of Gram-positive bacteria. In sequence similarity, five putatively novel species were identified with less than 98% similarity to other strains in the NCBI database. Tests for antimicrobial activities were performed against Gram-positive bacteria, Gram-negative bacteria, fungi, antitumor indicators Escherichia coli 343/591 (with DNA repair deficiency), regular E. coli 343/636 (with different DNA repair capacity), and 10 bacterial isolates exhibited inhibitory bioactivities. Among these strains, three isolates were detected involving function gene NRPS-A domains, which were most closely related to the amino acid sequences of linear gramicidin synthetase and pyoverdine synthetase. These results contribute to our knowledge of the microbes associated with marine sponges and further reveal novel bacterial resources for the screening of bioactive marine natural products.  相似文献   

基于COI部分序列探讨南海鲳属鱼类系统进化与种群遗传结构     

孙鹏  尹飞  施兆鸿  彭士明 《海洋科学》2012,36(6):15-21

利用线粒体细胞色素 c 氧化酶Ⅰ亚基(COI)部分序列研究了南海区鲳属鱼类(Pampus)银鲳(P. ar-genteus)、珍鲳(P. minor)和中国鲳(P. chinensis)的系统进化与种群遗传结构.通过 PCR 扩增与序列测定获得了长度为643 bp 的 COI 基因片段,其 A、T、G、C 碱基的平均含量分别为25.4%、33.6%、18.9%和22.1%, A+T 含量高于 G+C 含量.64条序列共定义了20种单倍型,包含152个变异位点,简约信息位点148个,单变异位点4个,产生169个点突变.结果表明,银鲳与中国鲳的遗传差异最小,银鲳与珍鲳的其次,而珍鲳与中国鲳的遗传差异最大,3种鲳属鱼类的遗传多样性均呈现较低的水平,应采取有效的保护措施,以避免其遗传多样性水平的进一步丧失.本研究结果为鲳属鱼类资源保护和合理开发利用提供必要的参考  相似文献   

中国的伤齿龙类恐龙化石     

沈才智  吕君昌  Martin KUNDRáT 《地球学报》2017,38(S1):73-74

伤齿龙类是手盗龙类中较为独特的一支, 时代从中侏罗世到晚白垩世, 主要分布于亚洲(东部和中部)以及北美地区。本文基于前人的研究成果, 结合新标本, 对中国伤齿龙类的系统分类、骨骼形态、骨组织、系统发育关系和古生物地理等方面进行了全面深入的评估与探讨。  相似文献   

烃降解菌假交替单胞菌N289的全基因组序列及其进化关系     

刘芳明  王以斌  曲长风  郑洲  缪锦来  许华  肖天 《海洋学报(英文版)》2017,36(2):88-93

假交替单胞菌属归属于交替假单胞菌科。交替假单胞菌N289菌株分离自南极海冰,我们通过二代测序技术获得了其全基因组序列,组装后获得2条染色体和1条质粒,大小分别是3.2M、636kb和1.8kb。全基因组共包含3,589个ORF,GC含量为40.83%。基因功能分析表明,该菌酶活性高,具有很强的环境适应性。这项研究有助于了解生物多样性、该菌株的进化地位和微生物相互作用关系。  相似文献