Abundance of fin whales (Balaenoptera physalus) in the north-western Mediterranean Sea,using photo-identification and microsatellite genotyping     

Céline Tardy  Denis Ody  Olivier Gimenez  Serge Planes 《Marine Ecology》2023,44(1):e12737

The Mediterranean fin whale population, Balaenoptera physalus, is resident, with almost no exchanges with the Atlantic population. The entire population was estimated at 1300 or 13,300 individuals by a recent project depending on the platform used. This disparity shows the importance of long-term monitoring with a unique protocol of survey. Capture-recapture approaches using dorsal photographs and genetic identity collections over a 10 years period were used to estimate the abundance of the north-western Mediterranean fin whale. We identified 332 individuals using photographs and 470 using genotypes, with a total of 546 individuals identified between 2008 and 2019, when some whales were double-marked. The inter-annual percentage of recapture varied between 15% and 17% respectively for genotypes and photographs methods. Using Cormack-Jolly-Seber models, the abundance of fin whales in the north-western Mediterranean is estimated at 1295 individuals (95% CI: 1116–1474) with a survival probability of 0.945 (95% CI: 0.690–0.993) from genotypes. Abundance estimates from combined collections (photographs and genotypes) and corrected photograph estimates were similar to the genetic ones. Future studies might prioritize the genetic approach which is the least biased and with a narrower confidence interval. The genetic abundance estimates show relative stability over time, when compared to 1990 estimates, and should be included in future conservation actions.  相似文献   

大珠母贝微卫星DNA标记的分离与筛选   总被引：2，自引：0，他引：2  

柳明  喻达辉  黄桂菊 《海洋科学》2010,34(8):1-5

采用生物素标记的(CA)15探针和磁珠富集法构建了大珠母贝(Pinctada maxima)微卫星DNA文库,随机挑选1200个菌落,经PCR筛选得到298个候选克隆,成功测序246个,分析获得251个微卫星序列,其中完美型、非完美型和混合型分别占63%、32%和5%。除探针中使用的CA重复外,还得到AT、GT、TC、AG、GC、TAA、CAA、AGG、GATA、GACA、GTGC、CGTC、GACG、CTGT等重复序列。设计引物90对,挑选其中30对合成并筛选出21对能在大珠母贝基因组有效扩增的引物。种群PCR扩增后利用变性聚丙烯酰胺凝胶电泳法进行检测,获得了10个多态位点,共检测出59个等位基因,片段长度范围为133~444 bp。各位点等位基因数2~8个,平均等位基因数5.9个;有效等位基因数为1.342 3~6.000 0,平均为4.124 0;多态性信息含量(CPI)为0.222 5~0.811 8,平均0.7179;期望杂合度(He)为0.259 3~0.847 5,平均0.717 9;观测杂合度(Ho)为0.300 0~0.800 0,平均0.533 3。这些微卫星多态性标记的获得,为进一步开展大珠母贝遗传育种、保护生物学和种群遗传多样性等研究奠定了一定基础。  相似文献