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161.
《青岛海洋大学学报(英文版)》2014,(2):236-242
Molecularly imprinted polymers (MIPs) were prepared by the bulk polymerization using crystal violet as the template molecule, and the methacrylic acid and ethylene glycol dimetheacrylate as functional monomer and cross-linker, respectively. Sys- tematic investigations of synthetic conditions were conducted. The surface morphology and recognition mechanism of the obtained polymers were studied using scanning electron microscope and spectrophotometric analysis. MIPs showed high atTmity to template molecule and were successfully applied as special solid-phase extraction sorbent for selective extraction of crystal violet from natural seawater. An off-line molecularly imprinted solid-phase extraction (MISPE) method followed by high-performance liquid chroma- tography with diodearray detection for the analysis of crystal violet was also established. MISPE columns have good recoveries for crystal violet standard solutions and good linearity was obtained over the concentration range of 0-200 ~tg L-1 (R2 〉 0.99). Finally, two natural seawater samples were investigated. The recoveries of spiked seawater on the MISPE columns were from 44.47% to 62.34%, the relative standard deviation (n=3) being in the range of 2.89%-5.96%. 相似文献
162.
Dogan Cetin Tayfun Sengul Shobha K. Bhatia Mahmoud M. Khachan 《Marine Georesources & Geotechnology》2017,35(5):678-687
Coal ash producing is an increasing trend because of its high energy demand worldwide. For transportation, disposal, and reuse of the industrial waste materials, geotextile tube’s dewatering technology has been widely used over the last three decades, which helps to decrease the volume of the dewatered slurry. In this study, effect of usage of polymer and fibers on dewatering characteristic of fly ash slurries was investigated. For the experimental investigation, an anionic polymer and short nylon fibers were used. As a new concept, centrifuge test is introduced as an alternative for the widely used pressure filtration test (PFT). Centrifuge test was used to evaluate final solid content of the retained sediments and change in slurry volume of fly ash. Tests were conducted on unconditioned and anionic polyacrylamide and/or fiber conditioned fly ash slurries. Centrifuge test results were compared with PFT results with respect to final solid content. It was found that fiber and/or polymer usage has remarkable effect on the dewatering rate of fly ash slurry. It was also found that final solid content of fly ash slurries was decreased by inclusion of fibers and polymer, which indicates that fiber and/or polymer usage can create more permeable soil body. 相似文献
163.
基于中型三轴仪开展了高聚物堆石料回弹模量试验,分析了高聚物堆石料卸载-再加载的力学特性和回弹模量变化规律。结果表明:围压和应力水平对体积变化规律有明显影响,随着试验围压的增大,试样由初始状态的剪胀逐渐变为剪缩;高围压下,随着应力水平的增大,试样的卸载体缩量呈非线性增大;而围压在100 kPa下,卸载时表现为体胀,不同应力水平下的卸载体缩量相近且较小。平均回弹模量与初始模量的比值大约在3.9~4.2之间,应力水平为0.7条件下对应的回弹模量与平均回弹模量较为接近。高聚物堆石料的回弹模量满足邓肯-张模型关系,数值计算过程中回弹模量系数近似为初始模量系数的4.0~4.2倍。 相似文献
164.
165.
本文采取传统的醇沉水溶的工艺提取了深海细菌的胞外聚合物(extracellularpolymericsubstance,EPS),使用过氧化氢作为损伤剂建立SH-SY5Y人神经母细胞瘤体外氧化损伤模型。在安全剂量下评价EPS的体外神经保护活性,确定16种活性良好(细胞活力>90%)的EPS。16种EPS的成分分析结果如下:1)16种EPS的分子量分布呈三段式,分别为>200 kDa, 10~200 kDa和<10 kDa; 2)16种EPS单糖组成中除甘露糖、葡萄糖外,还有稀有单糖氨基葡萄糖、鼠李糖、葡萄糖醛酸、氨基半乳糖、半乳糖、岩藻糖; 3)EPS的总糖含量为14.06%~21.40%,蛋白含量为10.66%~31.05%,硫酸基含量为2.91%~26.53%。通过对相应菌株进行16SrDNA全序列分析并与GenBank比对,完成了活性菌株的鉴定,通过MEGA7.0和Figtree构建系统进化树。鉴定结果表明,16株深海细菌归属于3门8科9属16种,分布在三个门类:厚壁菌门(Firmicutes, 9/16),变形菌门(Proteobacteria, 6/16)和... 相似文献