苯并（a）芘诱导下岩虫CYP基因的表达特征     

李婉娟  赵欢  Ba Fuyang  李绍娟  孙秀鹏  杨大佐  周一兵 《海洋学报(英文版)》2019,38(6):46-53

Rapid amplification of cDNA ends(RACE) and real-time polymerase chain reaction(RT-PCR) were carried out to analyze the CYP4 gene expression in polychaete Marphysa sanguinea exposed to benzo[a]pyrene(BaP) in this study. The full length of MsCYP4 cDNA was 2 470 bp, and it encoded 512 amino acids. The deduced amino acid sequence showed 47% identity with CYP4 F from frog Xenopus tropicalis and shared high homology with other known CYP4 sequences. To analyse the role of CYP4 in protecting M. sanguinea from BaP exposure, three BaP groups were established: 0.5, 5 and 50 μg/L. Polychaetes were sampled after 3, 7 and 12 d. At 0.5 μg/L, the effect of BaP on MsCYP4 gene expression increased with time prolonged. MsCYP4 gene expression curve showed Ushaped trend with time in 5 and 50 μg/L BaP groups. Therefore, MsCYP4 gene may play an important role in maintaining the balance of cellular metabolism and protecting M. sanguinea from BaP toxicity.  相似文献   

国土空间规划的海洋分区研究   总被引：2，自引：0，他引：2  

周鑫  陈培雄  黄杰  王权明 《海洋通报》2020,39(4):408-415

国土空间分区作为国土空间规划编制和自然资源统一管理的基础，对整个规划体系具有重要的支撑作用。在统一的国土空间规划体系下，用海分区既有其独立性与特殊性，也面临着陆海统筹、生态文明建设、海洋高质量发展等政策提出的 新要求。文章系统梳理了 1983 年以来海洋功能分区体系 4 次演变历程，结合国土空间规划改革对分区体系的内在需求，指出海洋功能分区体系存在未覆盖海域全域，分区层级的尺度、分工、内容和结构与国土空间规划的要求不一致，分区方案未考虑用途管制的需求，以及陆海统筹不足等问题。在剖析现有各类规划政策分区、管制分区和功能分区在国土空间规划体系中的定位和作用的基础上，提出以海洋主体功能区为基础，海洋生态红线为底线，以原海洋功能区划分区为主体，将无居民海岛纳入规划范围，并在生态、农业、城镇空间的大框架下统筹协调海陆分区，形成国土空间规划中的海洋分区体系。全国和省级国土空间规划统筹海陆主体功能，划定主体功能区，并建立海洋产业保障区名录、无居民海岛保护与利用名录和海洋自然保护地名录。市县级国土空间规划划定“功能+管制”的利用分区，共划分出海洋保护区、海洋发展区和海洋保留3个一级分区以及核心保护区、一般控制区、海水增养殖区等 12 个二级分区。  相似文献   

SAR图像海岸线检测的区域距离正则化几何主动轮廓模型   总被引：2，自引：0，他引：2  

姜大伟  范剑超  黄凤荣 《测绘学报》2016,45(9):1096-1103

合成孔径雷达(SAR)卫星遥感图像可以极大地提高全国海岸线覆盖频率,然而受到海洋波浪所引起的随机海水表面粗糙度的影响,海岸目标与海水背景边界易混淆不清,因此本文提出了基于区域距离正则化几何主动轮廓模型(RDRGAC),引入距离正则项,解决重复初始化水平集函数为符号距离函数的问题,提高了算法收敛速度。此外,将区域面积项系数与SAR图像等效视数(ENL)建立非线性拟合关系,实现RDRGAC模型根据不同SAR遥感图像的自适应调整,改善海岸线自动提取精度。通过河北省北戴河和大连市金州湾SAR数据海岸线提取对比试验,验证了所提方法的有效性。  相似文献   

Compression limit state of HVAC submarine cables     

《Applied Ocean Research》2016

An industry accepted standard does not currently exist for determination of compression limits in a subsea cable. This has resulted in most manufacturers specifying that subsea cables are not permitted to be axially loaded in compression.Additionally industry guidance does not exist regarding the consequences of inducing compression forces within subsea cables and the resulting effect on cable integrity. Industry recommended practice and guidance also does not have any information regarding experimental test arrangements to determine allowable compression levels within a subsea cable. This lack of modelling/testing guidance along with manufacturer recommendations of zero compressive loads within subsea cables results in overly conservative and restrictive design parameters for subsea cable installation and use.Due to the complex interaction within a subsea cable structure, such as contact interaction and friction between cable strands, theoretical modelling has been unable to provide reliable stress predictions and therefore an experimental testing regime is required if compression limits within the cable are to be appropriately determined. This paper describes combined axial and bending test arrangements that can be used as a guideline for determination of allowable compression limits for subsea cables.  相似文献   

基于长期观测的辽东湾口东部海域水动力特征研究   总被引：1，自引：0，他引：1  

赵骞  王梦佳  丁德文  陈伟斌 《海洋学报》2016,38(1):20-30

辽东湾口东部海域是辽东湾与渤海中部进行物质和能量交换的主要通道之一。本文利用坐底式海床基平台获取的近8个月的水动力连续观测资料,通过谱分析和调和分析方法对该海域的潮汐、潮流特征进行分析,并讨论了余流及底层温度的季节变化规律。研究结果表明:该海域潮汐属于不规则半日潮,平均潮差为0.95 m,最大可能潮差为2.27 m。潮流属于不规则半日潮流,M₂分潮流为其优势分潮流。主要分潮流运动形式为往复流,最大流速方向为西南-东北向。余流的季节性特征较为明显:秋季,余流流速在中层达到最大,流向以西南向为主;冬季,余流流速垂向变化较小,并呈西南偏西向流动;春季,流速随深度增加而减小,流向从表层至底层呈现逆时针旋转的特征。受底层潮流、水平温度梯度及海面温度日变化的影响,底层温度表现出短期的高频变化特征:秋季,短期振荡以半日周期信号为主;冬季,全日周期信号较为显著;春季,短期振荡的现象较弱。  相似文献   

基于面向对象影像分析方法的我国西沙珊瑚礁地貌单元信息提取   总被引：4，自引：1，他引：3  

徐京萍  赵建华  李方  王林  宋德瑞  文世勇  王飞  高宁 《海洋学报(英文版)》2016,35(12):19-27

珊瑚礁地貌单元的空间分布对于理解珊瑚礁生态系统的地质构造过程具有重要作用。然而,基于像素的影像分析方法往往获取不到较高精度的分类结果。本文基于面向对象的影像分析方法,利用Landsat 8卫星影像数据对我国西沙地区的珊瑚环礁进行了地貌单元的遥感信息提取。借鉴于美国千年珊瑚礁测绘项目的工作成果,本文首先针对研究区特点定义了十类珊瑚礁地貌单元类型。然后,基于对象的多层次关系特点,并综合利用对象的光谱、形状、上下文关系等特征,建立合适的分类规则集,获取了研究区较大尺度的珊瑚环礁地貌分区图,其分类精度普遍高于80%。虽然研究结果表明基于面向对象的影像分析方法可以有效的进行珊瑚礁遥感信息提取,但其规则集的可移植性仍需要今后的工作加以改善。  相似文献   

Determination of internal controls for quantitative gene expression of Isochrysis zhangjiangensis at nitrogen stress condition     

Shuang Wu  Jiannan Zhou  Xupeng Cao  Song Xue 《中国海洋大学学报(英文版)》2016,15(1):137-144

  相似文献   

Failure Pressure Analysis of Corroded Moderate-to-High Strength Pipelines     

苏晨亮  李昕  周晶 《海洋工程》2016,(1):69-82

Based on the elastic-plastic, large-deformation finite element method, burst capacity of steel pipeline with longitudinal corrosion defect subjected to internal pressure is studied. The appropriate stress-based criterion is used to predict the failure pressure of finite element model of corroded pipeline under internal pressure. By considering the pipe steel grades and geometries of corrosion defects, a series of finite element analyses is conducted. The effects of corrosion depth, length and width on burst capacity are also discussed. A specific failure pressure solution for the assessment of corrosion defects in moderate-to-high strength pipeline is proposed on the base of numerical results. The failure pressures predicted by the proposed method are in better agreement with the experimental results than the results by the other methods.  相似文献   

利用模拟退火算法反演多波束测量声速剖面          下载免费PDF全文

张志伟  暴景阳  肖付民  辛键 《武汉大学学报(信息科学版)》2018,43(8):1234-1241

针对多波束测量声速剖面站点布设密度不够而引起的声速剖面代表性误差问题,提出一种利用模拟退火算法反演声速剖面的方法。首先,对测区已有声速剖面序列进行经验正交函数（empirical orthogonal function,EOF）分析,利用声速扰动矩阵和前几阶EOF求得EOF重构系数及其变化范围。其次,采用模拟退火算法对EOF重构系数进行迭代优化,以多波束测得的海底地形畸变量大小为依据构建目标函数,并设定合理的退火控制参数。最后,得到待反演区域的声速剖面数据。实例分析表明,该方法反演的声速剖面较时间就近原则选取的替代声速剖面更接近真实声速剖面,且利用反演声速剖面改正后的海底地形更接近真实地形,有效削弱了声速剖面代表性误差的影响,显著提高了多波束测量精度及数据处理效率。  相似文献   

A new method for platform design based on parametric technology     

Yan-Yun Yu  Ming Chen  Yan Lin  Zhuo-Shang Ji 《Ocean Engineering》2010,37(5-6):473-482

A new method for platform design, named parametric platform design method (PPDM), is presented in this paper in order to improve the mobile platform design efficiency. In this method, an integrated parametric system that fully considers the characteristics of the platform is developed, which significantly optimizes the design process with the top–bottom design concept. In the parametric system, the main geometric dimensions are taken as parameters, while the topology between the structure members, the function requirements and the safety requirements are converted into geometric constraints. By geometric constraint solving (GCS), a set of parameters that satisfy all the given constraints are determined, and then the design scheme is obtained by several core algorithms, such as parametric tank subdivision, 3D stability calculation, parametric structure design, FEM preprocessing, etc. The parametric method greatly increases the changeability and the reusability of the platform model, and concurrent design is well supported. As the model is driven by parameters, PPDM is an excellent method for optimization design. As a result, PPDM has incomparable advantages on the design efficiency over traditional methods.  相似文献