Entrainment of E. coli and Listeria monocytogenes from sediment in irrigation canal     

《国际泥沙研究》2022,37(6):701-714

E. coli and Listeria monocytogenes (or L. monocytogenes) are bacteria affecting fresh produce that is harmful for health of humans and animals. If these bacteria are present in surface waterbody (e.g., irrigation canals), they will impair irrigation water quality and threaten produce safety. This paper studied the resuspension of E. coli and Listeria from bed sediment into irrigation water through several sets of laboratory experiments in an open channel flume. We studied three types of sediments using several flow rates in different velocities and shear stress. Bacteria's concentration in water increases with the bed shear stress. Two empirical relations were derived to correlate the concentration of E. coli and L. monocytogenes with the dimensionless bed shear stress. The experimental data favorably verified the relationships for sandy loam, loamy sand, and loam. The results showed that both bacteria could entrain from sand more efficiently compared to other sediments (i.e., sandy loam or loam). These relationships can be applied to water quality models for simulating E. coli and L. monocytogenes transport in irrigation canals for better managing irrigation water quality.  相似文献   

Geographical distribution of general aerobic heterotrophic bacteria in surficial sediments from the Chukchi Sea and Canadian Basin     

高爱国  陈皓文 《极地研究(英文版)》2007,18(2):147-154

This paper determined the abundance of General Aerobic heterotrophic Bacteria(GAB) in surficial sediments from the Chukchi Sea and the Canadian basin by using MPN and discussed their geographical distribution.The result shows that the determination percentages of the GAB were high,even till 100 percentage.The abundance range and averages of GAB for 4℃and 25℃ were from 4.00×102 to 2.40×106,1.71×106 ind.·g-1(wet sample) and from 2.40×105 to 2.40×107,1.10×107 ind.·g-1(wet sample) respectively.Not only the abundance range but also the averages of GAB in 25℃ were higher than that in 4℃.The abundance of GAB in sediments show a tendency that it is roughly greater in the lower latitudinal area than in the higher latitudinal area.The abundance of GAB increased from east to west as for the longitudinal distribution.With the water depth increasing,the abundance of GAB at 4℃ decreased,but GBA at 25℃ is not changed obviously with water depth.It seems that warmer circumstantial temperature is more suitable for some GAB.  相似文献   

Comparison of Enterococcus density estimates in marine beach and bay samples by real-time polymerase chain reaction, membrane filtration and defined substrate testing     

Ferretti JA  Tran HV  Cosgrove E  Protonentis J  Loftin V  Conklin CS  Grant RN 《Marine pollution bulletin》2011,62(5):1066-1072

Currently, densities of Enterococcus in marine bathing beach samples are performed using conventional methods which require 24 h to obtain results. Real-time PCR methods are available which can measure results in as little as 3 h. The purpose of this study was to evaluate a more rapid test method for the determination of bacterial contamination in marine bathing beaches to better protect human health. The geometric mean of Enterococcus densities using Enterolert® defined substrate testing and membrane filtration ranged from 5.2 to 150 MPN or CFU/100 mL and corresponding qPCR results ranged from 6.6 to 1785 CCE/100 mL. The regression analysis of these results showed a positive correlation between qPCR and conventional tests with an overall correlation (r) of 0.71. qPCR was found to provide accurate and sensitive estimate of Enterococcus densities and has the potential to be used as a rapid test method for the quantification of Enterococcus in marine waters.  相似文献   

Ecological Effects of Remote Smallsand Descending on Urban Area: Deduced from the "Mud - Snow" in Changchun City     

Wu Donghui  Hu Ke  Wang Yun  Yang Deming  Zhao Wei 《东北亚地学研究》2000,(2)

IntroductionContinued sandstorms occurring in North Chinaeally this year have attracted close attention of manyscientists from different aspects. It is recorded thatserious sandstorms occurred eight times in China in1960' s, thirteen times in 1970, s, fou…  相似文献   

Microbial response to surface microtopography: the role of metabolism in localized mineral dissolution     

Katrina J. Edwards  Andrew D. Rutenberg 《Chemical Geology》2001,180(1-4):19-32

We examine the role of microtopographical surface features on sulfide minerals in localizing and aligning bacterial adhesion. Experimental data shows strong correlation between bacterial cell alignment and principal crystallographic axes of pyrite (100 and 110). While bacteria often adhere to visible surface imperfections such as scratches, in many cases no associated surface features are visible. Additionally, the size of the surface imperfection does not unambiguously determine its effect in localizing and aligning bacterial cells. We theoretically model bacterial adhesion. We find that the depth of a surface feature such as a scratch is less important than its cross-sectional shape. Surface features that conform to the bacterial shape can strongly alter local bacterial adhesion energies, even with heights of only 10 nm. Hence, small local surface alterations due to bacterial metabolism could strongly affect local adhesion parameters, and may account for the observed bacterial distributions on mineral surfaces.  相似文献   

紫金山铜矿原地破碎细菌浸出工艺的可行性分析     

王观石胡世丽  艾幼孙 《江苏地质》2004,28(4):228-232

根据紫金山铜矿的实际情况，分析采用原地破碎细菌浸出工艺回收紫金山铜矿资源的工程技术可行性，在此基础上进行经济方面的讨论。  相似文献   

Reduzierung von Bakterien- und Coliphagen-Konzentrationen auf der Fließstrecke eines Klärwerksableiters     

S. Krner  H. Dizer  J. M. Lopez-Pila 《洁净——土壤、空气、水》1995,23(6):264-270

Reduction of the Concentration of Bacteria and Coliphages along the Flowing Stretch of a Treated Sewage Channel The efficiency of surface waters to eliminate E. coli, fecal streptococci, Salmonella spp., and coliphages was evaluated in a small river which receives treated wastewater and which is rich in submerged macrophytes. The study took place between April and December, 1994. Total colony count, BOD₅, O₂ concentration and water temperature were determined in the river as well. As the river does not receive additional water downwards along its 17.2 km course, dilution effects could be ruled out as the cause for the elimination of the microorganisms. The reduction is assumed to happen rather due to sedimentation, grazing, and adsorption to the submerged waterplants. Immediately after discharge of the wastewater, the river water contained about 10⁵ cfu/100 mL E. coli and 10⁴ cfu/100 mL fecal streptococci, about 1000 pfu/100 mL coliphages, and, as a rule, was positive for salmonella in 10 mL. The reduction of E. coli, fecal streptococci, salmonella, clostridia, and coliphages at the end of the course was 1 to 2 orders of magnitude. This reduction took place mainly within the first 4.7 km, a part in which, due to low flowing velocities, suspended solids settle down efficiently. Besides, at the end of this part the submerged waterplants are especially abundant. The reduction of suspended solids correlated positively with that of BOD₅, bacteria, and coliphages. The reduction of microorganisms was not sufficient to fulfill the requirements of the European Community guidelines for bathing waters and for surface waters used as drinking water source. The regenerating capacity of surface waters is not sufficient to eliminate pathogens from convenionally treated wastewater. Therefore, tertiary treatment is necessary to keep receiving waters reasonably free from pathogens.  相似文献   

The effect of distribution of iron-oxyhydroxide grain coatings on the transport of bacterial cells in porous media   总被引：3，自引：0，他引：3  

E. P. Knapp  Janet S. Herman  George M. Hornberger  Aaron L. Mills 《Environmental Geology》1998,33(4):243-248

 Among the demonstrated processes influencing the transport of bacteria through aquifers, the deposition of cells on mineral surfaces is one of the most important. For example, understanding the transport of introduced bacteria through aquifers is essential to designing some in situ bioremediation schemes. The impact of the presence and distribution of Fe(III)-oxyhydroxide-coated sand grains on bacterial transport through porous media was evaluated in column experiments in which bacteria (short rods; 1.2 μm length) were eluted through columns of quartz sand (0.5–0.6 mm in diameter) for several conditions of chemical heterogeneity of mineral substrate. Fe(III)-oxyhydroxide-coated sand was present as 10% of the mass, and it was arranged in three treatments: (1) homogeneously distributed, and present as a discrete layer (2) at the top and (3) at the bottom of 14-cm-long sand columns. A pulse input of 10⁸ cells ml^–1 was introduced in an artificial groundwater solution flowing at 14 cm h^–1 through the column, and eluted cells were counted. Peak breakthrough occurred at 1.0 pore volume. A large proportion of cells were retained; 14.7–15.8% of the cells were recovered after three pore volumes of solution had eluted through clean quartz sand, and only 2.1–4.0% were recovered from the Fe(III)-oxyhydroxide-coated sand mixtures. The three physical arrangements of the chemical heterogeneity resulted in essentially the same breakthrough of cells, indicating that the spatial distribution of iron coating does not affect the transport of bacteria. The results of the column transport experiments, which mimic hydrogeological conditions encountered in field problems, are consistent with our mechanistic understanding of bacterial sorption. Received: 10 April 1996 · Accepted: 17 February 1997  相似文献   

Microscale Response of Sediment Variables to Benthic Disturbance in the Central Indian Ocean Basin     

Shanta Nair  C. Mohandass  P. A. Loka Bharathi  G. Sheelu  Raghukumar Chandralata 《Marine Georesources & Geotechnology》2000,18(3):273-283

The response of sediment bacteria and biochemical variables to benthic disturbance was investigated in the Central Indian Ocean Basin from cores at nine stations. Generally, bacterial density and biochemical variables declined vertically with depth in the upper layers of the cores. Mechanical disturbance caused by a hydraulic disturber brought about a substantial decrease in total bacterial numbers. However, the numbers of retrievable bacteria increased by two orders of magnitude. The biochemical parameters decreased in quantity with a shift in the linear relationship. The data suggest that abiotic artifacts will directly affect the biomass and biochemistry of the sediment.  相似文献   

对虾养殖围隔生态系浮游动物对细菌的摄食作用   总被引：1，自引：0，他引：1  

刘国才  李德尚  董双林 《海洋科学进展》2000,18(1):45-49

1997年 6～ 9月在山东省海阳市黄海水产集团养虾场 ,采用围隔实验生态学方法研究了对虾养殖生态系浮游动物对细菌的摄食作用。结果表明 :浮游动物对细菌摄食量为 98～643μg C· dm- 3· d- 1,平均为 ( 2 62± 1 67)μg C· dm- 3· d- 1。浮游动物对细菌摄食量为细菌生产量的 73%～ 1 75% ,平均为 ( 1 1 3± 31 ) %。小于 3μm的浮游动物对细菌摄食量占浮游动物对细菌总摄食量的 1 7% ,其它规格浮游动物 ( >3μm)摄食量占 83%。  相似文献