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51.
Previous studies found intraspecific diversity in Scrippsiella trochoidea A. R. Loeblich III, a widely distributed calcareous cyst-producing dinoflagellate. In this study, three strains (ST-1, ST-D6
and ST-K) of S. trochoidea isolated from the East Asian waters were studied, together with other geographical strains, to resolve their phylogenetic
relationships. For the three East Asian isolates, two highly diverse regions of nuclear-encoded ribosomal DNA (rDNA), the
5.8S rDNA and its flanking internal transcribed spacers 1 and 2, and the 5′ portion of the large-subunit rDNA (encompassing
the “D1“ and “D2” domains) were sequenced. Homologous sequences from other geographical isolates were selected from the GenBank
database and the phylogenetic relationships were inferred from the molecular data of these strains. Strains of S. trochoidea were found to cluster into three major clades (STR1, STR2 and STR3), as reported in previous studies. Two of the three strains
ST-1 and ST-K, were grouped in clade STR2, the other strain, ST-D6, belonged to clade STR3. The intraspecific diversity of
S. trochoidea in East Asian waters makes it necessary to carry out phylogenetic investigations in combination with data of biogeography,
population dynamics, and life cycle on the ecophysiology of a region. 相似文献
52.
2016年以来,秦皇岛地区多次出现麻痹性贝类毒素导致的中毒事件,但对于该海域脂溶性藻毒素的污染状况仍不够了解。2020—2021年,本研究通过对秦皇岛近岸海域为期1年的现场采样调查,应用高效液相色谱-质谱联用手段检测了浓缩浮游植物和贻贝(Mytilus galloprovincialis)、牡蛎(Crassostrea gigas)和扇贝(Chlamys farreri)3种贝类中脂溶性藻毒素的污染状况,并通过高通量测序手段对浮游植物样品中的潜在有毒藻种进行了分析。结果表明,在秦皇岛近海浮游植物样品和贝类中均可检出扇贝毒素-2(pectenotoxin-2,PTX2),其中2021年4—5月浓缩浮游植物样品中的PTX2毒素含量达到最高值1.36ng/L,贝类中的PTX2毒素也主要在该时段检出,4月26日3种贝类中均可检出PTX2,含量最高达到1.10μg/kg。对浮游植物样品的高通量测序结果表明,样品中检测到的鳍藻序列数与毒素含量具有显著线性相关性,推测PTX2毒素主要来自样品中的渐尖鳍藻(Dinophysis accuminata)和倒卵形鳍藻(D.fortii)等。综合一年的调查结... 相似文献
53.
有害藻华是海洋中最为常见的一类生态灾害问题,对养殖业、旅游业和公众健康等造成了严重威胁,也给海洋管理带来了巨大的挑战。近年来,与有害藻华相关的生物、化学、水文、气象等观测数据以及有害藻华发生记录等统计数据不断积累,统计学方法、地理信息系统(Geographic Information System, GIS)等数据分析方法也在快速发展,为深入挖掘有害藻华时空演变特征、提高有害藻华监测预警水平奠定了重要基础。该研究综合应用数据库、地理信息系统及可视化技术,设计了一套集有害藻华多源异构数据管理、分析与可视化为一体的综合平台,能够实现对有害藻华相关数据的存储和动态关联,并集成了空间分析方法,实现了数据和分析产品的可视化。该研究以渤海有害藻华长期时空演变特征探究为案例,展示了该平台在有害藻华数据分析与可视化方面应用效果。该平台可望为公众科普、科学分析和政府决策等提供支撑。 相似文献