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《广东海洋大学学报》2016,(4)
采用DNA条形码技术辅以传统形态分类方法,对雷州半岛马尾藻属(Sargassum)6常见种进行物种鉴定和系统进化分析。测得6种马尾藻线粒体COI基因序列,该序列与Gen Bank和BOLD数据库中马尾藻序列同源性均大于或等于99%,该结果与形态分类学鉴定结果一致。COI基因序列特征分析表明,6种马尾藻保守位点421个,变异位点58个,简约信息位点21个,单一突变位点37个,变异率7.7%,T、C、A、G平均含量分别为39.1%、18.9%、19.1%、22.9%,A+T含量(58.2%)高于C+G含量(41.8%),UUU所编码的苯丙氨酸(F)使用频率最高,达12.5%,最大似然法估算的转换颠换比值为2.42,种间遗传距离多在0.030±0.013~0.100±0.016之间。聚类分析结果与形态学鉴定、同源性分析结果一致。 相似文献
74.
A New Combination and Two Varieties of Polystigmate Gomphonema (Gomphonemaceae Bacillariophyta) from Heilongjiang Province, China 总被引:1,自引:0,他引:1
The authors discribed a new polystigmate combination, Gomphonema turris Ehr. var. okamurae (Skv.) Fan et Bao.comb.nov., and two new varieties, Gomphonema tuttis Ehr. vat. latum Fan et Wang. var.nov. and Gomphonema acum/natum var. obtusum Fan et Bao, var. nov., that were distinguished by frustule morphology under LM. The samples were collected from freshwater of Heilongjiang Province, China. 相似文献
75.
Eighteen gametophytes including L.japonica ,L.ochotensis and L.longissima,were verified with random amplified polymorphic DNA (RAPD) technique.Eighteen ten-base primers were chosen from 100 primers selected for final amplification test.Among the total of 205 bands amplified ,181(88.3%) were polymorphic ,The genetic distance among different strains ranged from 0.072 to 0.391,The dendrogram constructed by unweighted pair -group method with arithmetic(UPGMA) method showed that the female and male gamtophytes of the same cell lines could be grouped in pairs respectively,It indicated that RAPD analysis could be used not only to distin-gusih different strains of Laminariz ,but also to distinguish male and female gametophyte within the same cell lines,There is ambiguous systematic relationship if judged mereely by the present data It seems that the use of RAPD marker is limited to elucidstion of the phylogenetic relationship among the species of Laminaria. 相似文献
76.
Genetic diversity and differentiation of the oyster Crassostreaplicatula populations from China's coast were studied basedon seven microsatellite loci.All loci showed high polymorphism for all five C.plicatula populations,with an average number of alleleper locus of 19.3-27.9 and an average expected heterozygosity of 0.889-0.952.Significant departures from Hardy-Weinberg equilib-rium and deficits of heterozygotes were observed over most populations at each locus,which were fully explained by null alleles.Microsatellite analysis revealed significant subdivision in the C.plicatula populations.According to the neighbor-joining tree con-structed on the basis of the D,A distance,the five populations fell into three regional groups,showing a relatively homogeneous geneticstructure in geographically close populations.Assignation tests correctly assigned high percentages of individuals to their originalpopulations and groups,and also confirmed the existence of genetic differentiation among C.plicatula populations.The results ob-tained in this study will facilitate the formulation of appropriate fisheries management programs,stock identification and conservationof biodiversity for the species. 相似文献
77.
描记采于曲靖市郊一地下水出水口金线鱼巴属一新种,命名为曲靖金线鱼巴Sinocyclocheilus qujingensis, sp.nov..新种口须特长,与长须金线鱼巴S. longibarbatus Wang(1989) 相近,但体鳞细小,隐于皮下,头长等于体高,两种间分子遗传距离13.6%,表明为亲缘较远的两个独立种. 相似文献
78.
Non-specific immune response of bullfrog Rana catesbeiana to pathogenic Aeromonas hydrophila was studied to 60 individuals in two groups. Each bullfrog in bacterium-injected group was injected intraperitoneally (i.p.) with 0.2 ml bacterial suspension at a density of 5.2 × 10^6 CFU/ml, while each one in control group injected i.p. with 0.2 ml sterile saline solution (0.85%, w/v). Three bullfrogs in both groups were sampled at 0, 1, 3, 7, 11, 15 and 20 days post-injection (dpi) for the evaluation of non-specific immune parameters. It was observed that intraperitoneal injection of A. hydrophila significantly increased the number of leucocytes and that of NBT-positive cells in peripheral blood. Significant increases in serum bactericidal activity and serum acid phosphatase activity were also observed in the bacterium-injected frogs when compared with those in the control group. However, a significant reduction was detected in vitro in phagocytosis activity of peripheral blood phagocytes. No significant difference in changes in the number of peripheral erythrocytes, serum superoxide dismutase (SOD) activity, and lysozyme activity was detected between the two groups. It is suggested that bullfrogs may produce a series of non-specific immune reactions in response to the A. hydrophila infection. 相似文献
79.
基于采自河北上侏罗统后城组的4件不完整角龙类标本建立了该类群的一个新属种,聂氏宣化角龙(Xuanhuaceratopsnieigen.etsp.nov.)。聂氏宣化角龙与另一晚侏罗世的角龙,发现于辽宁的杨氏朝阳龙共享许多进步特征,具有很近亲缘关系。但聂氏宣化角龙仅有一颗前上颌齿,不同于杨氏朝阳龙;另外,其方骨及肩胛骨也不同于杨氏朝阳龙。我们认为这两个分类单元代表角龙类的一个原始支系,发生于鹦鹉嘴龙与新角龙类这一阶元辐射之前,并为此建立朝阳龙科。中国河北上侏罗统后城组的一新角龙类@赵喜进$中国科学院古脊椎动物与古人类研究所!北京,100044,中国
… 相似文献
80.
摸索沙拐枣属(Calligonum L.)七种植物沙拐枣(Calligonum mongolicum Turcz. )、泡果沙拐枣(C. junceum (Fisch. et Mey.) Endl.)、无叶沙拐枣(C. aphyllum (Pall.) Gürke)、红果沙拐枣(C. rubicundum Bge.)、心形沙拐枣(C. cordatum E. Kor. ex N. Pavl.)、密刺沙拐枣(C. densum Borszcz.)、乔木状沙拐枣(C. arborescens Litv.)的总DNA提取方法并对DNA样品的纯度和浓度进行鉴定,对传统的CTAB法进行方法和材料的改进,开展与总DNA相关的PCR扩增和其他遗传学分析,并取得满意的结果。其结果对于沙拐枣属植物的有关研究提供了有效的实验手段。 相似文献