Application of the first internal transcribed spacer(ITS-1) of ribosomal DNA as a molecular marker to population analysis in farrer''s scallop Chlamys farreri     

YU Ziniu  WEI Xiaohu  KONG Xiaoyu  YU Shanshan 《海洋学报(英文版)》2007,26(1):93-100

Sequence variation of the first internal transcribed spacer of ribosomal DNA （ ITS - 1 ） was examined and its application to the study of genetic variation was explored in four populations of farter＇ s scallop Chlamys farreri. ITS - 1 fragments, with a length of about 300 bp,of 78 individuals collected from Dalian, Qingdao, Yantai in China and Korea respectively were amplified via PCR, cloned and sequenced. Intra-genomic variation was examined by sequencing several clones of single individuals. Alignment and polymorphism analysis detected 44 haplotypes and 50 polymorphic sites which consist of 30 substitutions and 20 indels, indicating a high level of polymorphisms. Sequence analysis also showed a very low level of intra-individual variation. All these features validated the feasibility of application of ITS - 1 fragment to population analysis. Polymorphism analysis showed that the Korea sample has the richest genetic variation, followed by Yantai and Qingdao samples. AMOVA （analysis of molecular variance） showed that the majority （96.26%） of genetic variation was distributed within populations and 3.74% resulted from among populations, but with P 〈 0.05 （ = 0.042）, indicating that the populations in this study have significant divergence. This output was basically concordant with the result arising from RAPD data and different from that from mitochondrial 16S rDNA sequence data. Discussion on this inconsistency was made accordingly.  相似文献   

莱州湾东岸海底地下水的水文地球化学特征及其地球化学演化模式   总被引：4，自引：0，他引：4  

李福林  陈学群  张奇  PETTIFER Geoff  宋福山  王潘平  刘学忠  姜克华  刘竹梅 《海洋科学进展》2005,23(2):190-196

莱州湾东岸的海底地下水主要分布在浅海区的第四纪松散沉积物中,明显有别于海水,目前已被大量开采利用。从内陆向海区分别取地下水样化验分析,其pH值、密度、钠吸附比(SAR)、rCl/rBr等化学特征值呈现出从陆向海逐渐变化的规律,内陆含水层地下水中较高的硝酸盐类污染物,在海底地下水中也有明显的反映,地下水的水化学类型也由ClCa·Na·Mg型转变为ClNa·Mg型和ClNa型。另一方面,海底地下水的主要组分、浓度和矿物相组成也有自己的特点。研究发现,从内陆向海区,地下水的离子强度和矿物质的饱和指数均呈现出一定的变化规律。地下水中的这些离子以及矿物相在空间上的水岩交换变化,使地下水逐渐从淡水演化为微咸水和咸水,构成了海岸带地下水地球化学演化的一般模式。鉴于海底地下水与内陆地下水的紧密联系,今后陆地含水层一旦被污染,地下水向海区的化学输移将带来一定的环境效应。这应当引起我们足够的重视。  相似文献   

西沙礁序列的磁性地层学研究   总被引：3，自引：0，他引：3  

张明书  周墨清 《海洋地质与第四纪地质》1996,16(3):61-65

利用西沙群岛西琛一井３３０ｍ以上井段的１４９块岩心标本，用美国２－Ｇ型超导磁力仪进行了古地磁测定，获得了一个从更新世至中新世晚期包括布容，松山，高斯和吉尔伯特四个极性时及若干亚极性的磁性地层柱，为礁序列的划分对比，提供了新的依据。  相似文献   

海洋调查水文资料异常值控制探讨     

梁广建  徐刚  龚建忠 《海洋测绘》2002,22(6):53-55

针对原始水文资料异常值的处理方法进行了初步探讨。证明了采用滤波和拟合的方法处理异常值具有很好的效果，既处理了异常值，又保留了一些小尺度变化。处理结果的好坏主要取决于滤波或拟合函数和偏差的选取。  相似文献   

三株赤潮硅藻5.8SrDNA及转录间隔区(ITS)的克隆及序列分析     

张宝玉  王广策  吕颂辉  齐雨藻  邹景忠  曾呈奎 《海洋学报》2006,28(1):111-117

对引发赤潮的3株硅藻——1株尖刺拟菱形藻(Pseudo-nitzshia pungens)和2株中肋骨条藻(Skeletonema costatum)的5.8SrDNA和ITS(internal transcribed spacers)序列进行了PCR扩增、克隆和序列测定,并分析了硅藻门10株赤潮藻(7株从GenBank获得)的系统进化关系.研究结果表明,尖刺拟菱形藻的ITS和5.8SrDNA的长度为693bp,SK-1(分离自东海赤潮暴发区)测序得到715bp,除ITS和5.8SrDNA外,还包含部分18SrDNA和28SrDNA;SK-2(分离自青岛养殖场)的ITS和5.8SrDNA的长度为331bp,尖刺拟菱形藻与从GenBank中获得的2株尖刺拟菱形藻相似程度最高,为100%,与该属的多列拟菱形藻相似程度稍低,为82.9%.SK-2的ITS序列与SK-1的相似程度很低,只有51%,但与拟中肋骨条藻的ITS序列相似程度高,为95.5%.SK-1的ITS序列与拟中肋骨条藻的相似程度也低,为56.7%.系统进化树反映的结果与相似性反映的结果一致.研究的该株尖刺拟菱形藻从根据ITS序列研究的结果与形态鉴定的结果看是一致的;SK-2可能属于拟中肋骨条藻;SK-1比较特殊,有待于用其他的方法进一步研究确定其分类地位.  相似文献   

ZQA2—1型海洋水文气象监测系统     

康寿岭  张齐 《海洋技术学报》1989,8(3):11-25

海洋水文气象监测是海洋环境研究和海洋开发的基础工作,而现代化的仪器设备又是提高监测水平的有效工具。ZQA2-1型海洋水文气象监测系统是以微型计算机为核心的自动监测装置。它可以自动、定时,长期地将所监测的海洋水文、气象要素进行打印和存贮,为用户提供实时和历史的资料。本文介绍了该系统的技术性能指标、基本构成与工作原理、应用程序等内容,并对其中有些技术问题作了较详细地论述。  相似文献   

山东日照近岸沉积物的重矿物分布与来源     

吕亚男 《山东海洋学院学报》1982,12(3):43-52

1978至1979年间，为了选建石臼所深水港，在进行海岸地质地貌和海洋水文调查的同时，对山东日照近岸带北起两城-白马河，南至绣针河口约480平方公里海域作了重矿物研究。取样最大水深为17米。本文根据80个样品极细砂粒级(0．125～0．063mm)的重矿物组成、  相似文献   

时间序列分析的一种新方法     

潘增宪 《海洋信息》1993,(11):20-21

  相似文献   

北太平洋中部热带海域深层水文状况分布     

徐炳荣 《海洋学研究》1990,8(2):1-6

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Genetic relationship between Neobenedenia girellae and N. melleni inferred from 28S rRNA sequences     

WANG Jun  ZHANG Wen  SU Yongquan  DING Shaoxiong 《海洋学报(英文版)》2004,23(4):709-716

The fragments of 350 bp in 28S rRNA from the closely related monogenea of trematoda, Neobenedenia girellae and N. melleni are obtained by polymerase chain reaction (PCR) amplified using a couple of special primers and then sequenced. The results show that the comparison of 28S rRNA sequences, with only a base varying in 337 bp accounting for 0.3% genetic difference, from the relative species N. girellae and N. melleni parasitized on the different fishes in different farms displays that they possess a very high genetic similarity of 99.7%, higher than that of 99.41% for the single species N. melleni sampled in different areas, and the intraspecific divergence of N.melleni is 0.59%. Meanwhile, the interspecific differences between the two Neobenedenia and three Benedenia (i.e., B. lutjani, B. rohdei and B. seriolae) range from 2.08% tol 1.73%. In addition, UPGMA and MP molecular phylogenetic trees are constructed and proved to be consistent with each other. Though the morphological characteristics and the results of genetic diversity for the two Neobenedenia show a high similarity, whether they belong to a single species or not are still undefined, and the more genes of them should be further investigated, in combination with the systematical and detailed morphological study.  相似文献