4种不同来源浒苔中多糖的提取分离及理化性质   总被引：2，自引：0，他引：2  

齐晓辉  李红燕  郭守东  陈荫  陈艳丽  徐健  侯玉娇  毛文君 《中国海洋大学学报(自然科学版)》2010,40(5)

为探讨不同季节以及不同地点采集的浒苔其多糖理化性质差异,采用水提醇沉法对黄海海域青岛沿海春(原料1)秋(原料3)两季采集的浒苔、2008年夏季爆发浒苔(原料2)以及东海海域福建沿海采集的浒苔(原料4)的多糖进行了提取,得到了4种冷水提取多糖PC1-4和4种热水提取多糖PH1-4。分别对8种浒苔多糖利用Q-Sepharose Fast Flow离子交换色谱法纯化后得到了12个组分。对各组分的总糖含量、糖醛酸含量、硫酸根含量以及蛋白含量进行了测定,利用气相色谱法测定了单糖组成,结果显示不同季节不同海域采集样品的化学组成及单糖组成不同。  相似文献   

Tide simulation using the mild-slope equation with Coriolis force and bottom friction     

LI Ruijie  JIANG Senhui  JIANG Bing 《海洋学报(英文版)》2010,29(6):44-50

Since the mild-slope equation was derived by Berkhoff (1972),the researchers considered various mechanism to simplify and improve the equation,which has been widely used for coastal wave field calculation.Recently,some scholars applied the mild-slope equation in simulating the tidal motion,which proves that the equation is capable to calculate the tide in actual terrain.But in their studies,they made a lot of simplifications,and did not consider the effects of Coriolis force and bottom friction on tidal wave.In this paper,the first-order linear mild-slope equations are deduced from Kirby mild-slope equation including wave and current interaction.Then,referring to the method of wave equations’ modification,the Coriolis force and bottom friction term are considered,and the effects of which have been performed with the radial sand ridges topography.Finally,the results show that the modified mild-slope equation can be used to simulate tidal motion,and the calculations agree well with the measurements,thus the applicability and validity of the mild-slope equation on tidal simulation are further proved.  相似文献   

天津地区连续GNSS时序观测值共模误差识别   总被引：2，自引：0，他引：2  

杨博  张风霜  韩月萍 《测绘科学技术学报》2010,27(5):319-323

为了恰当地识别共模误差并对观测结果进行修正,提出了均值-小波法.天津地区全球导航卫星系统(GNSS)时序观测资料的计算表明下列4点:1)在一定程度上共模误差是可识别和分离的;2)在局部区域上可较精确地剔出共模误差中的白噪声--闪烁噪声;3)共模误差在GNSS观测值误差中占主要成份,测站综合噪声、共模噪声和自身噪声的比例关系约为1:0.83:0.54;4)通过修正后观测值信噪比可显著提高.  相似文献   

Optimization of preparative separation and purification of total polyphenols from Sargassum tenerrimum by column chromatography     

Samee Haider  Zhenxing Li  Hong Lin  Khalid Jamil 《中国海洋大学学报(英文版)》2009,8(4):425-430

Polyphenols from the ethanol extracts of Sargassum tenerrimum (ST) with potent antiallergic effects were studied to optimize separation process through column chromatography. The adsorption and desorption characteristics of three widely used adsorbents: macroporous resin, silica gel, and polyvinylpolypyrrolidone (PVPP), were critically evaluated respectively and studied for the optimization of preparative separation of polyphenols. Static operations on these adsorbents showed that macroporous resin had the best adsorption and desorption capability among the three adsorbents. Dynamic adsorption and desorption with macroporous resin packed column were also conducted to optimize the parameters such as: with the optimal values shown in brackets, the concentration of extract solution (4 times diluted), pH value (6–7), adsorption speed (3 BV h⁻¹, bed volumes/per hour), concentration of ethanol (80%), elution speed (3 BV h⁻¹) and elution volume (7 BV). The chromatographic process so optimized gave a purity of 62.43% from the crude polyphenols, providing a promising basis for large scale preparation of bioactive polyphenols upon further scaling up tests.  相似文献   

Inheritance of 15 microsatellites in the Pacific oyster Crassostrea gigas： segregation and null allele identification for linkage analysis   总被引：2，自引：0，他引：2  

李莉  郭希明  张国范 《中国海洋湖沼学报》2009,27(1)

Microsatellites were screened in a backcross family of the Pacific oyster, Crassostrea gigas. Fifteen microsatellite loci were distinguishable and polymorphic with 6 types of allele-combinations. Null alleles were detected in 46.7% of loci, accounting for 11.7% of the total alleles. Four loci did not segregate in Mendelian Ratios. Three linkage groups were identified among 7 of the 15 segregating loci. Fluorescence-based automated capillary electrophoresis (ABI 310 Genetic Analyzer) that used to detect the microsatellite loci, has been proved a fast, precise, and reliable method in microsatellite genotyping.  相似文献   

渤海东部晚第四纪环境演变及潮流沙席沉积     

刘升发  庄振业  龙海燕 《海洋地质与第四纪地质》2008,28(1):25-31

通过对渤海东部CD5孔岩性、生物以及年代学的地层分析,晚第四纪以来划分出2个陆相和2个海相层,两次海侵的时间段分别为39～22和9kaBP至今。CD5钻孔附近渤海潮流三角洲大约在3600～2400aBP以来开始快速沉积,形成了4·8m厚的潮流沙席,结合渤海中东部10多个钻孔资料,总结了潮流沙席的环境演变过程,自全新世海侵以来可以大致分为9～8、8～3·6～2·4、3·6～2·4kaBP以来3个阶段,而目前正处于相对稳定状态。  相似文献   

Optimization of Preparative Separation and Purification of Total PolyphenoJs from Sargassum tenerrimum by Column Chromatography     

Samee Haider  LI Zhenxing  LIN Hong  Khalid Jamil 《青岛海洋大学学报(英文版)》2009,(4):425-430

Polyphenols from the ethanol extracts of Sargassum tenerrimum （ST） with potent antiallergic effects were studied to optimize separation process through column chromatography. The adsorption and desorption characteristics of three widely used adsorbents： macroporous resin, silica gel, and polyvinylpolypyrrolidone （PVPP）, were critically evaluated respectively and studied for the optimization of preparative separation of polyphenols. Static operations on these adsorbents showed that macroporous resin had the best adsorption and desorption capability among the three adsorbents. Dynamic adsorption and desorption with macroporous resin packed column were also conducted to optimize the parameters such as： with the optimal values shown in brackets, the concen- tration of extract solution （4 times diluted）, pH value （6-7）, adsorption speed （3BVh-1, bed volumes/per hour）, concentration of ethanol （80%）, elution speed （3 BV ht） and elution volume （7 BV）. The chromatographic process so optimized gave a purity of 62.43% from the crude polyphenols, providing a promising basis for large scale preparation of bioactive polyphenols upon further scaling up tests.  相似文献   

台风期间厦门湾潮流边界层特征研究     

潘伟然  骆智斌  张国荣  马腾 《台湾海峡》2009,28(4):540-545

本文基于2007年8月圣帕台风影响台湾海峡期间的厦门湾垂向海流流速剖面观测资料，运用Karman-Prandtl模型统计和分析了流速对数剖面的出现频率和边界层参数（摩阻流速与粗糙长度）．结果表明流速剖面满足对数分布出现频率都超过50％，但进行内部一致性原则分析后，流速剖面满足对数分布情况均不足30％．造成流速剖面偏离对数分布的因素主要有：台风、径流、流速加减速、水体分层、底床结构、海底悬沙浓度等等．台风期间的摩阻流速和粗糙长度都大于非台风期间的数值．  相似文献   

江苏辐射沙洲水道潮流调和分析   总被引：3，自引：1，他引：2       下载免费PDF全文

吴德安  崔效松  张忍顺  童朝锋 《海洋与湖沼》2008,39(2):105-111

利用改进的G·Godin潮流调和分析程序对辐射沙洲腹地水道中的29个测量站位连续2个潮周期准同步流速测量数据进行调和分析,得到9个分潮的调和常数及椭圆要素,对M2分潮潮流空间分布特征进行了分析。该海域M2分潮占优,潮流类型为正规半日潮。水道水流表现为往复流运动,主流向与岸线或水道的走向一致。流矢旋转在空间上表现较为复杂,在表层多体现为顺时针方向旋转运动。分析发现地形因素对中、底层旋转方向有较大影响。根据分析结果,对所测站位M2分潮潮流迟角和潮流浅水影响因子进行了计算分析。潮流浅水影响在沙脊及浅滩、浅沟上相对较大,浅水分潮影响随水深向下而增大。  相似文献   

地基土的随机地震反应的一个解法     

黄义  韩建刚 《世界地震工程》2005,21(2):13-17

利用连续线性系统的随机振动理论，研究非均匀地基土层地震的动力反应问题。假定土层的剪切模量随深度线性变化，得到土层的最大位移反应的均值和方差。引入结构微分算子，采用模态叠加法进行反应分析，与普通的分离变量法相比较，此方法概念明确、步骤简单。  相似文献