白洋淀超微真核藻的空间分布特征及关键影响因子     

雷瑾  史小丽  张民  李胜男  陈开宁  杨州 《湖泊科学》2019,31(6):1559-1569

超微真核藻个体微小、比表面积大,具有高效的碳吸收速率,对水体初级生产力具有重要的贡献.目前对淡水超微真核藻的认知还非常有限.于2017年5月对白洋淀进行采样调查,结合流式细胞术和高通量测序技术探究了白洋淀超微真核藻群落结构的空间分布特征及关键环境影响因子.结果表明,白洋淀超微真核藻的平均丰度为7.59×104cells/ml,且随着营养水平的升高呈现先增加后降低的变化趋势.回归分析表明,超微真核藻丰度在中营养水平水域主要受溶解性总磷和TN/TP影响,在富营养水域主要与盐度有关.测序比对结果表明在纲水平上白洋淀超微真核藻主要以绿藻纲Chlorophyceae、硅藻纲Bacillariophyceae、中心硅藻纲Coscinodiscophyceae、金藻纲Chrysophyceae为主,不同区域差异不大.但在操作分类单元(Operation Taxonomy Units,OTU)水平上,超微真核藻群落结构在白洋淀不同营养状态湖区存在显著差异,中营养水域主要类群为栅列藻科Scenedesmaceae,近囊胞藻属Paraphysomona sp.,定鞭藻纲Haptophyceae和甲藻纲Dinophyceae为主,而富营养水域主要类群为红球藻科Haematococcaceae,金藻纲的Chromulinale sp.和Chrysophycea sp..Bioenv分析表明,对超微真核藻群落组成影响最大的环境因子是溶解性总氮、溶解性总磷、TN/TP、硝态氮、溶解氧.本研究表明超微真核藻的群落结构存在较明显的环境异质性,对白洋淀水体富营养化状态有很好的指示.  相似文献   

Analysis of photosynthetic picoeukaryote community structure along an extended Ellett Line transect in the northern North Atlantic reveals a dominance of novel prymnesiophyte and prasinophyte phylotypes     

Amy R. Kirkham  Ludwig E. Jardillier  Ross HollandMikhail V. Zubkov  Dave J. Scanlan 《Deep Sea Research Part I: Oceanographic Research Papers》2011,58(7):733-744

Photosynthetic picoeukaryotes (PPEs) of a size <3 ??m can contribute significantly to primary production. Here, PPE community structure was analysed along an extended Ellett Line transect, an area in the North Atlantic well studied by physical oceanographers but largely neglected in the field of microalgal ecology. Distribution patterns of specific PPE classes were determined using dot-blot hybridization analysis, while the taxonomic composition of specific PPE classes was revealed by phylogenetic analysis of plastid 16S rRNA gene sequences. In addition, we performed fluorescent in situ hybridization (FISH) analysis of seawater samples collected along the transect to provide a PCR-independent survey of class level PPE distribution patterns. We found the PPE community was dominated by members of the Prymnesiophyceae, Prasinophyceae and Mamiellophyceae. Interestingly, phylogenetic analysis revealed several novel Prymnesiophyceae and Prasinophyceae phylotypes (with only 85-96% identity to neighbouring sequences) within lineages for which cultured counterparts are unknown.  相似文献   

2006年秋季长江口及邻近海域微微型浮游生物的分布特征及环境影响因素   总被引：3，自引：0，他引：3  

乐凤凤  刘诚刚  郝锵  史君贤  翟红昌 《海洋学研究》2011,(3):108-117

2006年10月在长江口及邻近海域采用流式细胞技术测定了微微型浮游生物:聚球藻Synechococcus、微微型光合真核生物(picoeukaryotes)和异养浮游细菌(heterotrophic bacteria)的丰度和碳生物量,研究了其生态分布特点,并分析了其与环境因子之间的关系.结果表明,聚球藻、微微型光合真...  相似文献   

Diurnal variation in relative photosynthetic performance of marestail (Hippuris vulgaris Linn.) Across a water temperature gradient using PAM fluorometry in Jiuzhaigou National Nature Reserve, Sichuan Province, China     

Carla BOSSARD 《山地科学学报》2011,8(6):794-807

This paper reports the first photosynthetic study of marestail in Jiuzhaigou. In this work, we used PAM fluorometry to examine photosynthetic rates of submerged and emerged marestail in three lakes. Three lakes were studied across a gradient of water temperature, with low water temperature conditions in Grass Lake and Arrow Bamboo Lake, and higher water temperature in Five Colored Lake. In the field, electron transport rates (ETR_max) were measured as rapid light curves (RLCs) by in situ yield measurements. Submerged and emerged marestail showed higher photosynthetic activity in Five Colored Lake compared to the other lakes, a response consistent with the adaptation of marestail in Five Colored Lake to high water temperature. The optimal temperature for photosynthesis of submerged marestail in Jiuzhaigou was about 12 °C. Nonphotochemical quenching (NPQ) of submerged and emerged marestail increased with increasing water temperature. Maximum quantum yield (F _v/F _m) of submerged marestail in Five Colored Lake showed full recovery at 1700 h due to higher NPQ. Further, the chlorophyll a for submerged marestail was the highest in Grass Lake and the lowest in Five Colored Lake. These results indicate that in different lakes the function of these aquatic plants is associated with a diversity of place-dependent environmental conditions, especially water temperature that leads to pronounced differences in the plant’s ecophysiological reactions.  相似文献   

冬季大鹏澳海域微表层浮游植物色素特征的日变化研究     

陈飞羽  江涛  张玲  王小冬  马长江  王朝晖 《海洋科学》2016,40(7):91-99

于2013年12月3日清晨、正午、傍晚采集了大亚湾大鹏澳海域3个站位的微表层和次表层水样,经过三级分级过滤(小型:20μm;微型:2.7~20μm;微微型:2.7μm)后,对其进行高效液相色谱(HPLC)色素分析,通过藻类色素化学分类法(CHEMTAX)分析不同浮游植物对Chl a的贡献,研究了微表层及次表层光合色素粒径特征及浮游植物群落结构差异。结果表明,冬季大亚湾海域水体中存在的浮游植物光合色素主要有17种,以岩藻黄素和Chl a含量较高。微表层总Chl a平均浓度为0.797μg/L,略高于次表层的0.714μg/L,不存在显著性差异(P0.05);微表层和次表层Chl a含量清晨最高,傍晚次之,正午最低。微表层不同粒径浮游植物对Chl a的贡献率从大到小依次为小型、微型、微微型浮游植物,分别为80.7%,10.1%和9.2%。CHEMTAX分析结果得出,冬季该海域硅藻占绝对优势,甲藻、定鞭藻、青绿藻、蓝藻、隐藻所占比重相差不大。微表层中定鞭藻、青绿藻和蓝藻等较小粒径浮游植物种群所占比重高于次表层,说明相对于次表层,微表层中的浮游植物群落有小型化趋势。  相似文献   

Temporal variability of Pb fluxes and bioturbation in shelf sediments beneath the high primary production area off Concepción, central-southern Chile (36°S)     

Praxedes Muñoz  Javier Sellanes  Carina Lange  Maritza Palma 《Progress in Oceanography》2007,75(3):586-602

We estimated monthly fluxes of ²¹⁰Pb in shelf sediments beneath a high productivity area off central-southern Chile (36°S) during 1 year (September 2002-August 2003). Sediment cores were obtained using a multiple corer and were analyzed mainly for ²¹⁰Pb, total pigments, and macrofauna abundance. The ²¹⁰Pb inventories and fluxes were estimated for surface sediments (0-5 cm) and bioturbation coefficients were inferred using chlorophyll-a (reactive) profiles. In general, ²¹⁰Pb content was inversely correlated with phytodetritus fluxes. High photosynthetic pigment contents in surface sediments were consistently associated with lower ²¹⁰Pb contents. Macrofaunal activity responded to oxygen and organic matter supplies at the sediment surface, generally concentrated in the first centimeters, but particularly so during months of high organic matter fluxes and deficient bottom water oxygen conditions. At this study site, several processes involved in the ²¹⁰Pb surface distribution make it difficult to accurately estimate ages at the surface. We postulate that the organic fluxes promote changes in the faunal activity, which, in combination with sediment resuspension and water circulation over the shelf, produce seasonal variations in the ²¹⁰Pb inventories.  相似文献   

Spring and summer phytoplankton communities in the Chukchi and Eastern Beaufort Seas   总被引：2，自引：0，他引：2  

Victoria Hill  Glenn Cota  Dean Stockwell 《Deep Sea Research Part II: Topical Studies in Oceanography》2005,52(24-26):3369

Phytoplankton pigments and size-fractionated biomass in the Chukchi and Beaufort Seas showed spatial and temporal variation during the spring and summer of 2002. Cluster analysis of pigment ratios revealed different assemblages over the shelf, slope and basin regions. In spring, phytoplankton with particle sizes greater than 5 μm, identified as diatoms and/or haptophytes, dominated over the shelf. Smaller (<5 μm) phytoplankton containing chlorophyll b, most likely prasinophytes, were more abundant over the slope and basin. Due to extensive ice cover at this time, phytoplankton experienced low irradiance, but nutrients were near maximal for the year. By summer, small prasinophytes and larger haptophytes and diatoms co-dominated in near-surface assemblages in largely ice-free waters when nitrate was mostly depleted. Deeper in the water column at 1–15% of the surface irradiance larger sized diatoms were still abundant in the upper nutricline. Phytoplankton from the shelf appeared to be advected through Barrow Canyon to the adjacent basin, explaining similar composition between the two areas in spring and summer. Off-shelf advection was much less pronounced for other slope and basin areas, which are influenced by the low-nutrient Beaufort gyre circulation, leading to a dominance of smaller prasinophytes and chlorophytes. The correlation of large-sized fucoxanthin containing phytoplankton with the higher primary production measurements shows promise for trophic status to be estimated using accessory pigment ratios.  相似文献   

几种红藻和蓝藻的光合作用色素   总被引：2，自引：0，他引：2  

仵小南  张锦东  张学成  臧汝波 《海洋科学》1992,16(6):39-44

分离纯化出几种海产红藻和一种蓝藻的光合作用色素,并测定了它们的化学性质和光谱学性质。这些藻类是3种红藻:多管藻(Polysiphonia urceolata)、橡叶藻(Phycodrys sp.)和条斑紫菜(Porphyra yezoensis);蓝藻:钝顶螺旋藻(Spirulina platensis)。用羟基磷灰石柱层析法从上述藻类中分离到几种不同的藻胆蛋白。经SDS-PAGE及光谱测定,发现条斑紫菜中的藻红蛋白不同于其它两种红藻。而橡叶藻中存在的两条藻红蛋白也有差异,条斑紫菜和钝顶螺旋藻中的两种别藻蓝蛋白之间也有区别。叶绿素分析表明,钝顶螺旋藻中叶绿素a的含量高于红藻中叶绿素a的含量。  相似文献   

Relationship of photosynthetic carbon fixation with environmental changes in the Jiulong River estuary of the South China Sea, with special reference to the effects of solar UV radiation   总被引：2，自引：0，他引：2  

Li G  Gao K  Yuan D  Zheng Y  Yang G 《Marine pollution bulletin》2011,62(8):1852-1858

Phytoplankton cells in estuary waters usually experience drastic changes in chemical and physical environments due to mixing of fresh and seawaters. In order to see their photosynthetic performance in such dynamic waters, we measured the photosynthetic carbon fixation by natural phytoplankton assemblages in the Jiulong River estuary of the South China Sea during April 24-26 and July 24-26 of 2008, and investigated its relationship with environmental changes in the presence or the absence of UV radiation. Phytoplankton biomass (Chl a) decreased sharply from the river-mouth to seawards (17.3-2.1 μg L⁻¹), with the dominant species changed from chlorophytes to diatoms. The photosynthetic rate based on Chl a at noon time under PAR-alone increased from 1.9 μg C (μg Chl a)⁻¹ L⁻¹ in low salinity zone (SSS < 10) to 12.4 μg C (μg Chl a)⁻¹ L⁻¹ in turbidity front (SSS within 10-20), and then decreased to 2.1 μg C (μg Chl a)⁻¹ L⁻¹ in mixohaline zone (SSS > 20); accordingly, the carbon fixation per volume of seawater increased from 12.8 to 149 μg C L⁻¹ h⁻¹, and decreased to 14.3 μg C L⁻¹ h⁻¹. Solar UVR caused the inhibition of carbon fixation in surface water of all the investigated zones, by 39% in turbidity area and 7-10% in freshwater or mixohaline zones. In the turbidity zone, higher availability of CO₂ could have enhanced the photosynthetic performance; while osmotic stress might be responsible for the higher sensitivity of phytoplankton assemblages to solar UV radiation.  相似文献   

蓖齿眼子菜的光合速率及影响因素   总被引：8，自引：2，他引：8  

陈开宁  强胜  李文朝 《湖泊科学》2002,14(4):357-362

本文研究了蓖齿眼子菜(Potamogeon pectinatus L)在滇池的光合速率、体内叶绿素季节变化及光和水温对其光合速率影响,并根据实验,计算出了蓖齿眼子菜生长的光补偿点,研究结果表明,蓖齿眼子菜1～3月份的净光合产氧量最大,为1.82～1.83mg/(g·h),6月份最低,为0.63 mg/(g·h);植物体内Chl.a和Chl.b平均含量分别为0.25～1.15mg/(g·h)和0.28～0.85mg/(g·h),蓖齿眼子菜可以通过调节体内Chl.a/Chl.b值来适应不同季节生长;蓖齿眼子菜有较宽的水温适应范围,10℃时的净光合产氧量可达0.88mg/(g·h),最高值出现在水温25℃,为1.16mg/(g·h),而30℃以上的水温已表现出对其生长的不利影响;蓖齿跟子菜生长的光补偿点为358～1256Lx,并随温度上升而逐渐增加.  相似文献