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地物的光谱反射率,是遥感图象分析的基础和依据。同样,藻类的光谱反射率R_∞,是遥感海洋叶绿素的基础和依据,也是影响海洋光谱特性的重要因素。 在海面上,用野外分光光度计测定某种藻类、某种浓度的光谱反射率是最直接的方法。但是,海上测定受到气象、海况、太阳高度、船舶颠簸、仪器使用条件等多种因素的影响,实验数据分布离散,工作条件差。在实验室测 相似文献
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几种红藻和蓝藻的光合作用色素 总被引:2,自引:0,他引:2
分离纯化出几种海产红藻和一种蓝藻的光合作用色素,并测定了它们的化学性质和光谱学性质。这些藻类是3种红藻:多管藻(Polysiphonia urceolata)、橡叶藻(Phycodrys sp.)和条斑紫菜(Porphyra yezoensis);蓝藻:钝顶螺旋藻(Spirulina platensis)。用羟基磷灰石柱层析法从上述藻类中分离到几种不同的藻胆蛋白。经SDS-PAGE及光谱测定,发现条斑紫菜中的藻红蛋白不同于其它两种红藻。而橡叶藻中存在的两条藻红蛋白也有差异,条斑紫菜和钝顶螺旋藻中的两种别藻蓝蛋白之间也有区别。叶绿素分析表明,钝顶螺旋藻中叶绿素a的含量高于红藻中叶绿素a的含量。 相似文献
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Calculation and comparative study of the chlorophyll fluorescence ratio F685/F735 in brown algae (Laminaria japonica, Underia pinnatifida and Padina crassa) excited by blue and green light showed that the fluorescence ratios were higher when the algae were excited by blue light (440 nm), but reduced obviously under green light (540 nm) excitation. The values also reduced under dehydration but could recover during rehydration if the stress was not serious. The variation of the fluorescence ratio under dehydration was mainly because changes in fluorescence emission at 735 nm were always sharper than those at 685 nm. The ratio was sensitive to stress and has potential as a stress indicator in phycological research. Measurement of the fluorescence excitation spectra showed that the only peak at 540 hm changed apparently during dehydration. It meant that the function of the Chl a /Fucoxanthin protein complex for energy transfer was easily inhibited by water stress. However, no variation of the ratio was foun 相似文献
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目前,在钴的测定方法中,多数还是应用分光光度法,所以,合成灵敏度高和选择性好的显色剂仍然是人们十分注意的问题。 相似文献
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近几年来,为了探索一种灵敏度高、选择性好的测定钴的试剂,人们对呲啶偶氮类化合物进行了研究。Shibata等首先将4-(2-呲啶偶氮)-1,3-二氨基苯(PADAB)及其卤代化合物合成并测定了钴和钯,相继又有人进行了研究。 相似文献
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The chorophyll fluorescence ratio F685/F735 in vivo can be a useful indicator for stress detection inhigher plants and seaweed. DCMU [3 - (3, 4 - dichlorophenyl ) - 1, 1 - dimethylurea] treatment influencesthis ratio. Th effects of DCMU on F685/F735 of marine red, brown and green algae under excitationlight of different wavelengths were investigted. In the brown algae, Laminaria japonica and Undariapinnatifida,DCMU did not increase this ratio under blue light excitation but increased the ratio slightlyunder excitation by green light. For the red algea, Halymenia sinensis, DCMU increased the ratiomarkedly under both blue and green ligh excitation. The percentage increase could reach 50% (undergreen light excitation) and was due to unequal enhancement at the two emission maxima by DCMU. Afraction of chlorophyll which contributed to fluorescence in the 735 nm region was less sensitive toDCMU and was likely from photosystem I of red algae. In the green alga, Ulva pertusa, DCMU causeda slight increase in F685 相似文献
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