拟穴青蟹(Scylla paramamosain)六个地理群体肌肉氨基酸和脂肪酸的比较分析     

李永亮  赵欢欢  李荣华  王春琳  母昌考  宋微微  叶央芳 《海洋与湖沼》2019,50(2):465-472

本文对广西钦州(Gq)、福建霞浦(Fx)、浙江温州(Zw)、浙江三门(Zs)、浙江宁海(Zn)、浙江慈溪(Zc)等6个野生群体拟穴青蟹(Scylla paramamosain)肌肉进行了氨基酸及脂肪酸分析。共测定出19种游离氨基酸和26种脂肪酸。氨基酸含量在80.94—191.39mg/g之间, Gq为最高;总脂肪酸含量在7.97—11.38mg/g之间,Zn为最高。主要氨基酸为甘氨酸和精氨酸;主要脂肪酸为C20:5n3(EPA),占总脂肪酸含量的平均值为(22.47±5.84)%。各群体风味氨基酸和多不饱和脂肪酸含量丰富,占总氨基酸比例(TDAA/TFAA)和总脂肪酸比例(PUFA/TFA)分别为(53.62±5.43)%和(59.27±5.29)%。Zs群体的必需氨基酸含量及C22:6n3(DHA)均为最高。主成分分析结果表明:各群体的氨基酸与脂肪酸组成与含量相似性与地理距离分布具有一定的相关性,特别是Gq群体,在氨基酸与脂肪酸的分类中与其他群体区分比较明显。各群体风味氨基酸和多不饱和脂肪酸的聚类分析结果均表明:Zw、Zc与Gq群体较为接近,具有较强的甜味; Fx、Zn与Zs群体较为接近,具有较强的香味及不饱和脂肪酸营养价值。  相似文献   

EFFECT OF LIGHT INTENSITY ON THE TOTAL LIPID AND FATTY ACID COMPOSITION OF SIX STRAINS OF MARINE DIATOMS     

梁英麦康森  孙世春于道展 《中国海洋湖沼学报》2001,19(3):249-254

The effect of light intensity ( 1500 Ix and 5000 Ix) on the total lipid and fatty acid composition of six strains of marine diatoms Cylindrotheca fusiformis (B211 ), Phaeodactylum tricornutum (Bl14, Bl18 and B221) Nitzschia closterium (B222) and Chaetoceros gracilis (B13) was investigated.The total lipids of B13, Bl14, and B211 grown at 5000 Ix were lower than those grown at 1500 Ix. No evident changes were observed in Bl18, B221 and B222. Fatty acid composition changed considerably at different light intensity although no consistent correlation between the relative proportion of a single FA and light intensity. The major fatty acids of the 6 strains were 14:0, 16:0, 16:l(n- 7) and 20:5(n-3) . Cylindrothecafusiformis had high percentage of 20:4n - 6 (9.2 - 10.9% ) . The total polyunsaturated fatty acid /rl all 6 strains decreased with increasing light intensity. The percentage of the highly unsaturated fatty acid eicosapentaenoic acid (EPA) decreased with increasing light intensity in all strains except Chaetoceros gracilis.  相似文献   

Fatty Acid Composition Analyses of the DCMU Resistant Mutants of Nannochloropsis oculata (Eustigmatophyceae)     

ZHANG Jimin )  LIU Shuang )  SUN Xue )  YANG Guanpin )  ZHANG Xuecheng )  *  GAO Zhenhui ) ) North China Sea Monitoring Center  SOA  Fushun Road  Qingdao  P..R.China) College of Marine Life Sciences  Ocean University of China  Qingdao  P..R.China 《中国海洋大学学报(英文版)》2003,2(1)

1　Introduction　Nannochloropsissp .areoneofpotentialsourcesofpolyunsaturatedfattyacids ,especiallytheeicosapen taentaenoicacid (EPA ,C2 0∶5 ) ,whichisimportantforbothanimalsandhumans .　Thefattyacidcompositionofthisalgaisaffectedbyvariousenvironmentalfactors ,suchaslightintensity(Sukeniketal.,1989;Sukeniketal.,1990 ) ,imply ingthatafactorinfluencingthephotosyntheticprocessmayaffectcellularfattyacidsynthesisandtheirmetabolism .AselectionprogramofincreasingcellularEPAcontenthasbeencarriedo…  相似文献   

鱼粉脂肪酸组成的分析研究   总被引：7，自引：1，他引：7  

宋永康  林虬  陈人弼 《台湾海峡》2004,23(1):88-93

本文应用气相色谱对水产饲料的主要原料——— 4类进口鱼粉的脂肪酸组成进行分析 ,结果表明 :①鱼粉中主要脂肪酸含量SFA为C1 6∶0、C1 8∶0 ,MUFA为C1 8∶1、C1 6∶1 ,PUFA为EPA和DHA .② 4类鱼粉中 ,美国、新西兰、俄罗斯的鱼粉MUFA含量较为接近 ,都在 4 0 %左右 ,但新西兰鱼粉的C1 8∶1含量较美国鱼粉高 ,其平均值分别为 2 50 %和 1 9.53% ,而C 2 0∶1、C2 2∶1 ,则比美国低一些 ,俄罗斯鱼粉的含量居两者之间 .智利鱼粉的MUFA比前 3种鱼粉低 ,只有 30 %左右 .③ 4类鱼粉中新西兰鱼粉的PUFA最低 ,它们的EPA与DHA的比值为 :美国鱼粉接近 1∶1 ,新西兰鱼粉为1∶1 .5～ 1∶3.2 ,俄罗斯鱼粉为 1∶1 .2～ 1∶1 .4 ,智利鱼粉为 1∶2左右 ,④智利鱼粉的C2 2∶5n 3明显高于其它 3类鱼粉 ,而C2 0∶1、和C2 2∶1则明显低于其它 3类鱼粉  相似文献   

Total Lipid and Fatty Acid Classes in Decomposing Mangrove Leaves of <Emphasis Type="Italic">Bruguiera gymnorrhiza</Emphasis> and <Emphasis Type="Italic">Kandelia candel</Emphasis>: Significance with respect to Lipid Input     

Prosper?L.?Mfilinge  Tarik?Meziane  Zainudin?Bachok  Makoto?TsuchiyaEmail author 《Journal of Oceanography》2005,61(3):613-622

Changes in the concentration of total lipid and fatty acids (FAs) during the decomposition of mangrove leaves were investigated by field experiments using yellow leaves of Bruguiera gymnorrhiza (L.) Lamk. and Kandelia candel (L.) Druce, in order to quantify mangrove contribution to lipid and fatty acid inputs to marine sediments. Total lipid and total FA in the fresh (green and yellow) and decomposing leaves of both species were significantly higher during winter than summer. During decomposition, total lipid content and FA concentration, in particular branched chain fatty acids (BrFAs) and bacterial fatty acids (BFAs), increased to a maximum concentration in 45 days during winter and in 17 days during summer. Lipids were lost faster in K. candel leaf detritus than in B. gymnorrhiza leaf detritus in which >90% of the total lipid original weight was lost during the summer experiment and <60% during the winter experiment. The changes in the concentrations of total lipids and FAs in the decomposing leaves also indicate that mangrove leaves are significant sources of fatty acids and probably other lipid compounds to estuarine ecosystems and that tidal waters transport the lipids and FAs adsorbed to particulate matter from mangroves to adjacent estuarine sediments and the ocean.  相似文献   

不同温度的有机溶剂分提对鯷鱼鱼油得率及脂肪酸组成的影响     

朱碧英  毛秀珍  毋瑾超  陈全震 《海洋学研究》2004,22(1):70-75

讨论了不同温度的有机溶剂分级提取对鳀鱼鱼油得率、脂肪酸组成及EPA、DHA含量变化的影响。经15℃干法分级提取的液态鳀鱼鱼油,在设定的4种温度下,液态鳀鱼鱼油得率均随溶剂量的增加而提高;在相同溶剂量的情况下,随着分级提取温度的降低,液态鳀鱼鱼油得率呈下降的趋势。有机溶剂分级提取对14碳短碳链的饱和与不饱和脂肪酸及16碳、18碳的饱和脂肪酸的脱除效果是明显的,但对脱除20碳、22碳的长碳链饱和脂肪酸的效果不明显。在4种试验温度下,EPA和DHA的总量分别为29.88%、31.74%、35.27%和41.80%,高出美国阿拉斯佳深海鱼油对应总量(28.76%)的1.12%～13.04%。  相似文献   

The application of isotopic and biogeochemical markers to the study of the biochemistry of organic matter in a macrotidal estuary, the Loire, France   总被引：1，自引：0，他引：1  

A. Saliot  J. Tronczynski  P. Scribe  R. Letolle 《Estuarine, Coastal and Shelf Science》1988,27(6)

The biogeochemistry of organic matter in a macrotidal estuary, the Loire, France, has been studied for two years during different seasons. Both particulate matter and sediment have been sampled in the riverine zone, in the maximum turbidity zone and in the ocean near the river mouth. Two techniques have been used: carbon isotopic ratio determination and analysis of lipid-marker signatures in the n-alkane, n-alkene and fatty acid series. For the period corresponding to the output of the maximum turbidity zone in the ocean, the complete change of organic matter, continental in nature in the inner estuary, pure marine in the outer estuary is well illustrated by the decrease of δ¹³C values and of carbon preference index of n-alkanes. Input sources of organic matter by continental plants, plankton and micro-organisms are discussed from biogeochemical-marker analyses data along with the processes of accumulation of particles and their evolution with the season. Some criteria for evidencing the nature of various organic-matter pools are assessed and compared in different chemical-marker series as follows: high molecular weight n-alkanes and fatty acids, perylene for continental imprints, polyunsaturated 18-, 20- and 22-carbon fatty acids, n-C₁₇, n-alkenes and squalene for algae imprints, branched iso and anteiso fatty acids, Δ11-C_18:1 for microbial imprints.  相似文献   

Sugar and Lipid Components in Sea Foam near Kelp Beds     

Branko  Velimirov 《Marine Ecology》1982,3(2):97-107

Abstract. A component analysis of the sugar and lipid fraction in foam layers around kelp beds on the South African west coast showed the following trends: In the residue of foam drainage of both 12 h and 120 h old foam the sugar fraction is represented by mannose (33–41 %), galactose (4–30%), fucose (3–29%) and glucose (19–26%). With the exception of fucose, the same components could be found in foam filtration. In the lipid fraction the main fatty acids are palmitic (23.3–27.3%), oleic (14.0–5.5%) and timnodonic acid (11.2–9.1%). Selacholeinic acid was only found in 12 h old foam while lignoceric acid could not be located in foam filtration. Against expectation mannitol was not identified in quantifiable amounts in foam samples although it is the dominant component of kelp mucilage, whose presence is of major importance for foam formation. Hypotheses explaining the lack of mannitol are forwarded. Component sugar and lipid content for 11 of foam was calculated and estimation of sugar and lipid content in an average foam patch (volume of 30001) is given. The importance of foam formation as a mechanism for binding and transfer of energy in the marine environment is emphasized.  相似文献   

超声波对湛江等鞭金藻生长和脂肪酸组成的影响   总被引：5，自引：0，他引：5  

李文权  王宪  陈清花  张元标  翁蓁洲 《海洋学报》2002,24(3):94-100

设计了一个超声频率、超声功率和处理时间三因素四水平的正交实验,研究了超声波对湛江等鞭金藻的生物学效应.实验结果表明,超声频率对湛江等鞭金藻的生物学效应显著,而超声功率和处理时间的影响相对较小.对于提高湛江等鞭金藻生长速率常数,最佳超声条件为20kHz,6W,10s3次;对于提高其脂肪酸不饱和度和主要不饱和脂肪酸百分比,最佳超声条件为20kHz,4～6W,30s.在最佳超声条件下,湛江等鞭金藻生长速率常数最高可达0.630d,是对照组的2.02倍,其脂肪酸不饱和度最高可达79.6%,比对照组提高7.8%,其中主要不饱和脂肪酸的百分含量均得到不同程度的提高.  相似文献   

Variation in microbial biomass and community structure in sediments of peter the great bay (sea of japan/east sea), as estimated from fatty acid biomarkers     

Natalia V. Zhukova 《Ocean Science Journal》2005,40(3):34-42

Variation in the microbial biomass and community structure found in sediment of heavily polluted bays and the adjacent unpolluted areas were examined using phospholipid fatty acid analysis. Total microbial biomass and microbial community structure were responding to environmental determinants, sediment grain size, depth of sediment, and pollution due to petroleum hydrocarbons. The marker fatty acids of microeukaryotes and prokaryotes - aerobic, anaerobic, and sulfate-reducing bacteria -were detected in sediments of the areas studied. Analysis of the fatty acid profiles revealed wide variations in the community structure in sediments, depending on the extent of pollution, sediment depth, and sediment grain size. The abundance of specific bacterial fatty acids points to the dominance of prokaryotic organisms, whose composition differed among the stations. Fatty acid distributions in sediments suggest the high contribution of aerobic bacteria. Sediments of polluted sites were significantly enriched with anaerobic bacteria in comparison with clean areas. The contribution of this bacterial group increased with the depth of sediments. Anaerobic bacteria were predominantly present in muddy sediments, as evidenced from the fatty acid profiles. Relatively high concentrations of marker fatty acids of sulfate-reducing bacteria were associated with organic pollution in this site. Specific fatty acids of microeukaryotes were more abundant in surface sediments than in deeper sediment layers. Among the microeukaryotes, diatoms were an important component. Significant amounts of bacterial biomass, the predominance of bacterial biomarker fatty acids with abundance of anaerobic and sulfate-reducing bacteria are indicative of a prokaryotic consortium responsive to organic pollution.  相似文献