米氏凯伦藻细胞表面膜蛋白质组及其对温度变化的响应研究     

王坤  王沛  张浩  张树峰  王大志 《海洋与湖沼》2019,50(3):652-663

米氏凯伦藻(Karenia mikimotoi)近年在我国福建、浙江和广东沿海经常形成赤潮,其赤潮不仅影响到海洋生态系统的稳定,也严重威胁到水产养殖以及人类生命健康安全。本论文以米氏凯伦藻为研究对象,建立了米氏凯伦藻细胞表面膜蛋白质荧光标记技术和细胞膜蛋白质提取方法,运用荧光差异凝胶电泳技术(2-DDIGE)对膜蛋白质进行了分析,并研究了米氏凯伦藻的膜蛋白质组及其对环境温度变动的响应。实验共鉴定到44个细胞表面膜蛋白,其中有效注释27个,主要为转运蛋白、HSP70蛋白家族和捕光蛋白等。米氏凯伦藻在20°C条件下的细胞生长和光合作用要明显好于16°C和12°C,但16°C和12°C条件下的差别不大,表明低温限制了米氏凯伦藻的生长。当米氏凯伦藻从12°C快速转移至16°C和20°C时,藻细胞密度和光合作用效率短时间迅速降低,但细胞很快即适应温度变化。细胞膜上的转运蛋白和光合作用蛋白在其适应温度变化中起着重要作用。  相似文献   

秦岭造山带多金属矿普查复杂地层护壁堵漏技术          下载免费PDF全文

柳硕林  韩明耀 《探矿工程》2020,47(1):43-47

秦岭造山带多金属矿普查项目钻探施工过程中钻遇多种复杂地层，垮塌、缩径、漏失等复杂情况频发，严重影响了施工效率。分别采用了植物胶钙处理钻井液、聚乙烯醇无固相钻井液等进行护壁工作，结合不同漏失情况分别实施了随钻堵漏、凝胶堵漏等技术措施，取得了良好的使用效果。  相似文献   

延迟交联凝胶研制及其在广西某铀矿堵漏应用          下载免费PDF全文

熊正强  陶士先  刘俊辉  李晓东  薛倩冰 《探矿工程》2020,47(4):140-144

针对地质钻探小井眼条件下失返性漏失堵漏技术难题，开展了适用于地质钻探堵漏要求的聚合物交联凝胶研究。采用单因素优选法，优选了交联凝胶体系用各种处理剂，并以环保型植物胶GJ为成胶剂，加入交联控制剂N1及交联剂1号等组分，研制了一种延迟交联凝胶。该凝胶在广西某铀矿勘查ZK6-37-1孔堵漏成功应用，解决了2个孔段失返性漏失问题。研究结果表明，该凝胶具有交联时间可控(交联时间10～75 min)以及较高的凝胶强度（承压≥6 MPa），在解决失返性漏失方面具有广阔的应用前景。  相似文献   

高聚物外包盾构隧道离心机振动台试验研究     

马雪  王复明  郭成超  张继春 《地震工程与工程振动》2022,42(1):180-187

为了验证非水反应聚氨酯高聚物(后文简称高聚物)外包盾构隧道具有减震性能,对高聚物外包盾构隧道模型和无外包隧道模型,采用离心机振动台进行对比试验。针对中密砂土围岩,在不同阵型特征的地震波输入下,对比有无高聚物外包隧道加速度、频谱、隧道动力响应和地表沉降,分析高聚物的减震性能。试验结果表明:在不同地震激励的工况下,对于有无高聚物外包的隧道模型,其加速度和频谱差别不大;二者隧道各点上的动应变平均相差31.32%;高聚物外包层隧道模型地表的最终沉降分别比无外包隧道模型和自由场地表沉降约小20%和50%。试验证明高聚物对盾构隧道有减震作用,此试验研究为高聚物在今后的工程应用提供参考依据。  相似文献   

Drilling Technology of the M-1 Well in Hot Dry Rock of Matouying,Tangshan北大核心CSCD   总被引：1，自引：0，他引：1  

张云  高亮  刘现川  赵岩  王德  张建永 《地质与勘探》2022,58(1):176-186

This paper presents the general situation and key techniques of the drilling well M-1 in Matouying dry hot rock exploration hole, Leting, Tangshan, Hebei Province. A series of problems have been studied on the sea water drilling fluid, high temperature resistant fresh water drilling fluid, screw composite drilling technique, screw hole coring technology, and high temperature, thus solving the difficulty of sea water drilling fluid well protection, poor stability of drilling fluid under high temperature, and low efficiency in hot dry rock drilling and coring drilling to ensure the safety of the borehole and improve drilling efficiency. The high temperature resistant double condensation and double density cement slurry system and relevant technical measures are adopted to ensure the quality of cementing in the high temperature environment. Through the understanding and analysis of the strata in this area, this work provides valuable experience for drilling in the same strata in the future. The final hole depth of the M-1 well in Matouying is 4502. 11m, and hot dry rock with a temperature of 150t is found at 3965 m, which is the first hot dry rock well with a temperature of more than 150°C in the Beijing-Tianjin-Hebei region so far. © 2022 Editorial Board of Geology and Exploration. All rights reserved.  相似文献   

青藏高原复杂地层地质钻探低固相冲洗液试验研究          下载免费PDF全文

袁进科  陈礼仪  王军伟  乔友浩 《探矿工程》2021,48(4):79-84

青藏高原由于地质条件的多样性,分布有松散破碎地层、高应力地层、断层破碎带等复杂地层,在地质钻探中经常遇到塌孔、漏浆、缩径、卡钻、埋钻等问题。同时配套使用的冲洗液形成的泥皮质量差、胶结能力较差、携岩粉能力较弱等不足。冲洗液的性能是实现复杂地层顺利钻进的关键。结合青藏高原复杂地层的地质特点,研发了低固相聚合物新型冲洗液,该冲洗液以钠质膨润土和微泡剂等外加剂为基础,具有流动性好、胶结性强、降失水量适中的特点,适宜在青藏高原复杂地层地质钻探中使用。  相似文献   

The role of hydrothermal fluids in sedimentation in saline alkaline lakes: Evidence from Nasikie Engida,Kenya Rift Valley     

Robin W. Renaut  R. Bernhart Owen  Tim K. Lowenstein  Gijs De Cort  Emma Mcnulty  Jennifer J. Scott  Anthony Mbuthia 《Sedimentology》2021,68(1):108-134

Saline alkaline lakes that precipitate sodium carbonate evaporites are most common in volcanic terrains in semi‐arid environments. Processes that lead to trona precipitation are poorly understood compared to those in sulphate‐dominated and chloride‐dominated lake brines. Nasikie Engida (Little Magadi) in the southern Kenya Rift shows the initial stages of soda evaporite formation. This small shallow (<2 m deep; 7 km long) lake is recharged by alkaline hot springs and seasonal runoff but unlike neighbouring Lake Magadi is perennial. This study aims to understand modern sedimentary and geochemical processes in Nasikie Engida and to assess the importance of geothermal fluids in evaporite formation. Perennial hot‐spring inflow waters along the northern shoreline evaporate and become saturated with respect to nahcolite and trona, which precipitate in the southern part of the lake, up to 6 km from the hot springs. Nahcolite (NaHCO₃) forms bladed crystals that nucleate on the lake floor. Trona (Na₂CO₃·NaHCO₃·2H₂O) precipitates from more concentrated brines as rafts and as bottom‐nucleated shrubs of acicular crystals that coalesce laterally to form bedded trona. Many processes modify the fluid composition as it evolves. Silica is removed as gels and by early diagenetic reactions and diatoms. Sulphate is depleted by bacterial reduction. Potassium and chloride, of moderate concentration, remain conservative in the brine. Clastic sedimentation is relatively minor because of the predominant hydrothermal inflow. Nahcolite precipitates when and where pCO₂ is high, notably near sublacustrine spring discharge. Results from Nasikie Engida show that hot spring discharge has maintained the lake for at least 2 kyr, and that the evaporite formation is strongly influenced by local discharge of carbon dioxide. Brine evolution and evaporite deposition at Nasikie Engida help to explain conditions under which ancient sodium carbonate evaporites formed, including those in other East African rift basins, the Eocene Green River Formation (western USA), and elsewhere.  相似文献   

高浓度化学浆液的研制与应用效果          下载免费PDF全文

朱效中  朱学光 《探矿工程》2011,38(10):60-62

淮北某煤矿因第四含水层水量过大,通过F5断层导入采煤工作面,使采煤生产陷入停顿。为解决采煤工作面涌水量过大问题,研制了高浓度化学浆液,经钻孔注浆收到良好的止水效果,使近200万t煤炭资源恢复开采,取得了很好的经济和社会效益。  相似文献   

冻土区天然气水合物勘探低温钻井液理论与试验   总被引：3，自引：2，他引：1       下载免费PDF全文

杨葳  杨阳  徐会文 《探矿工程》2011,38(7):29-31,56

天然气水合物是在特定的低温与高压条件下形成的产物。在天然气水合物勘探工作中,低温钻井液是获得天然气水合物真实样品的重要保证条件之一。低温钻井液应具有低的冰点、良好的抵制能力与良好的流动性。结合天然气水合物勘探工作的特点,在试验的基础上,对比分析了PAM、PHPA、PAC-141、Na-CMC与KHm的分子结构、官能团的种类与数量对钻井液的防塌能力和流动性的影响,得出了几种处理剂的耐低温能力大小的顺序为：PAC-141〈PHPA〈PAM〈Na-CMC〈KHm,为天然气水合物勘探中低温钻井液的配制与使用,奠定了重要的基础。  相似文献   

弱冻胶调剖剂在孤岛油田污水中成胶性能评价     

宋岱锋  张岩  薄纯辉  李楠  陈辉 《海洋地质译丛》2011,(2):62-64,90

应用孤岛油田现场聚合物和美国进口复合有机铬交联剂TIORCOWC684进行了一系列的室内评价实验。实验结果表明,宝莫聚合物均具有较好的初始黏度、且形成的弱冻胶体系强度高;弱冻胶主段塞选取聚合物浓度2500--3000mg／L,交联剂浓度300--600mg／L,成胶强度较高,且胶体不易脱水;弱冻胶封口段塞聚合物浓度为4000mg／L,交联剂浓度300--900mg／L,胶体强度较大、韧性好,满足封口的要求;同时评价了封口段塞的耐盐性能,结果表明该体系随盐类矿化度的增加体系强度有增加趋势。  相似文献