大豆酶解蛋白对凡纳滨对虾幼虾生长性能、血清生化指标、非特异性免疫力和抗病力的影响     

王铵静  杨奇慧  谭北平  肖伟伟  贾静  董晓慧  迟淑艳  刘泓宇  章双 《广东海洋大学学报》2018,(1)

【目的】研究大豆酶解蛋白对幼虾生长性能、血清生化指标、非特异性免疫力和抗病力的影响。【方法】凡纳滨对虾(Litopenaeus vannamei)基础饲料添加质量分数0(对照)、1.0%、1.5%、2.0%、2.5%、3.0%、3.5%和4.0%大豆酶解蛋白,配制8种等氮等脂饲料,饲喂凡纳滨对虾幼虾56 d。【结果】1.5%组的增重率和特定生长率最高,显著高于2.0%—4.0%添加组(P0.05);1.0%和1.5%组全虾粗蛋白质含量高于对照组(P0.05),对照、1.0%和1.5%组蛋白质沉积率高于其他各组(P0.05);饲料中添加1.5%~4.0%大豆酶解蛋白可显著提高肌肉磷含量(P0.05),3.0%组最大;1.0%、1.5%和2.0%组血清胆固醇含量低于对照组(P0.05);饲料中添加1.5%~4.0%大豆酶解蛋白可显著提高血清中溶菌酶活性(P0.05),添加1.0%~3.0%大豆酶解蛋白可显著提高血清中酚氧化物酶活性(P0.05),2.5%和3.0%组血清中超氧化物歧化酶活性与1.0%和1.5%组血清中酸性磷酸酶活性高于对照组(P0.05),添加大豆酶解蛋白可显著提高血清中碱性磷酸酶活性(P0.05),2.5%组最大;哈维弧菌(Vibrio harveyi)攻毒7 d后,1.0%组累积死亡率最低。饲料中添加大豆酶解蛋白未提高凡纳滨对虾的生长性能,添加剂量高于3.5%可导致生长性能下降,添加质量分数1.0%大豆酶解蛋白可明显提高凡纳滨对虾的抗病力。【结论】饲料中添加大豆酶解蛋白不能提高凡纳滨对虾的生长性能,高于3.5%添加组的生长性能下降;添加质量分数1.0%大豆酶解蛋白可明显提高凡纳滨对虾的抗病力。  相似文献   

Silicon limitation on primary production and its destiny in Jiaozhou Bay, China——Ⅳ：Study on cross-bay transect from estuary to ocean   总被引：1，自引：0，他引：1  

Yang Dongfang  Chen Yu  Gao Zhenhui  Zhang Jing  Wang Fan 《中国海洋湖沼学报》2005,23(1):72-90

The authors analyzed the data collected in the Ecological Station Jiaozhou Bay from May 1991 to November 1994, including 12 seasonal investigations, to determine the characteristics, dynamic cycles and variation trends of the silicate in the bay. The results indicated that the rivers around Jiaozhou Bay provided abundant supply of silicate to the bay. The silicate concentration there depended on river flow variation. The horizontal variation of silicate concentration on the transect showed that the silicate concentration decreased with distance from shorelines. The vertical variation of it showed that silicate sank and deposited on the sea bottom by phytoplankton uptake and death, and zooplankton excretion. In this way, silicon would endlessly be transferred from terrestrial sources to the sea bottom. The silicon took up by phytoplankton and by other biogeochemical processes led to insufficient silicon supply for phytoplankton growth. In this paper, a 2D dynamic model of river flow versus silicate concentration was established by which silicate concentrations of 0.028–0.062 μmol/L in seawater was yielded by inputting certain seasonal unit river flows (m³/s), or in other words, the silicate supply rate; and when the unit river flow was set to zero, meaning no river input, the silicate concentrations were between 0.05–0.69 μmol/L in the bay. In terms of the silicate supply rate, Jiaozhou Bay was divided into three parts. The division shows a given river flow could generate several different silicon levels in corresponding regions, so as to the silicon-limitation levels to the phytoplankton in these regions. Another dynamic model of river flow versus primary production was set up by which the phytoplankton primary production of 5.21–15.55 (mgC/m²·d)/(m³/s) were obtained in our case at unit river flow values via silicate concentration or primary production conversion rate. Similarly, the values of primary production of 121.98–195.33 (mgC/m²·d) were achieved at zero unit river flow condition. A primary production conversion rate reflects the sensitivity to silicon depletion so as to different phytoplankton primary production and silicon requirements by different phytoplankton assemblages in different marine areas. In addition, the authors differentiated two equations (Eqs. 1 and 2) in the models to obtain the river flow variation that determines the silicate concentration variation, and in turn, the variation of primary production. These results proved further that nutrient silicon is a limiting factor for phytoplankton growth. This study was funded by NSFC (No. 40036010), and the Director's Fund of the Beihai Sea Monitoring Center, the State Oceanic Administration.  相似文献   

Microsatellite Variation in the Oyster Crassostreaplicatula Along the Coast of China     

YU Hong  LI Qi YU Ruihai 《中国海洋大学学报(英文版)》2008,7(4)

Genetic diversity and differentiation of the oyster Crassostreaplicatula populations from China's coast were studied basedon seven microsatellite loci.All loci showed high polymorphism for all five C.plicatula populations,with an average number of alleleper locus of 19.3-27.9 and an average expected heterozygosity of 0.889-0.952.Significant departures from Hardy-Weinberg equilib-rium and deficits of heterozygotes were observed over most populations at each locus,which were fully explained by null alleles.Microsatellite analysis revealed significant subdivision in the C.plicatula populations.According to the neighbor-joining tree con-structed on the basis of the D,A distance,the five populations fell into three regional groups,showing a relatively homogeneous geneticstructure in geographically close populations.Assignation tests correctly assigned high percentages of individuals to their originalpopulations and groups,and also confirmed the existence of genetic differentiation among C.plicatula populations.The results ob-tained in this study will facilitate the formulation of appropriate fisheries management programs,stock identification and conservationof biodiversity for the species.  相似文献   

The comparison between allozyme and RAPD makers for the population genetic structure analysis of scallop Chlamys farreri     

刘保忠 尤锋董波  相建海 《中国海洋湖沼学报》2006,24(3):295-299

1INTRODUCTION Since the first use in human genetic research in1966,allozyme has become the most popular genetic marker for population genetics studies for almost all groups of animals and plants(Lewontin and Hubby,1966;Murphy et al.,1996).In the past seve…  相似文献   

盐泽螺旋藻变种的生长和生化特性   总被引：1，自引：0，他引：1  

钱凯先  侯盛颖 《海洋与湖沼》1996,27(4):357-361

采用１９８４年在美国加利福尼亚大学培育成功的盐泽螺旋藻变种，经扫描电镜，生物量测定和生化分析，进一步研究其基本特性。结果表明，其藻丝呈紧密的逆时针向等宽螺旋结构。  相似文献   

南海带鱼遗传变异及其分类的研究   总被引：4，自引：0，他引：4  

王可玲  刘兰英  尤锋  徐成 《海洋科学》1992,16(2):69-72

带鱼是我国海洋鱼类中最重要的经济鱼类,过去进行了大量的研究,多认为中国近海的带鱼为一个种,而不同的学者分别认为存在3,4和5个种群。从1976年开始,我们试图主要用生化方法并结合形态学资料研究带鱼的分类。1983和1988年报道了我们通过带鱼肌浆蛋白和形态学部分资料的研究认为中国近海带  相似文献   

应用微核技术监测海水诱变剂的初步实验   总被引：2，自引：0，他引：2  

方宗熙马德修  林光恒侯家龙戴继勋  周汝伦陈登勤欧毓麟  崔竞进王梅林 《山东海洋学院学报》1981,11(2):74-79

工业化和环境污染总是并行发生的。如何在工业化过程中尽量避免环境污染是大家关心的问题。在研究这个问题中，建立有效的生物测定法，来监测环境污染，防止污染物影响人类健康是很有意义的。  相似文献   

中华乌塘鳢的群体生物学研究 Ⅱ.生化遗传变异     

李刚 《热带海洋学报》1991,(2)

用同工酶谱法发现中华乌塘鳢Bostrichthy sinensis四个群体的遗传变异水平很低,在所检测的30个基因座位中,多态座位比例只有0—10％,平均杂合度仅为0.2—0.5％,物种的平均杂合度为0.33±0.10％。四个群体共享常见的等位基因。虽然其中三个群体拥有特有的等位基因,但其基因频率都未超过5％。四个群体的Nei氏遗传距只有1.68—12.13×10~(-5)。据分析,近交可能是遗传变异水平低和形态特征分化差的主要原因。种苗生产上应注意广泛采集不同地理群体的鱼作亲鱼,以丰富养殖群体的基因库。  相似文献   

几种养殖鲍同工酶生化遗传的比较研究   总被引：8，自引：0，他引：8  

柯才焕  陈晓佳  周时强  王志勇  王艺磊 《台湾海峡》2003,22(2):173-179,T002

运用聚丙烯酰胺凝胶电泳对我国主要养殖鲍(皱纹盘鲍、盘鲍及九孔鲍)12种同工酶(LDH、ME、MDH、GPI、PGM、IDH、IDDH、SOD、EST、ODH、ADH、AAT)26个基因座位进行了分析．结果表明：EST、PGM可以作为区分三种(亚种)鲍的生化遗传标记；在所分析的位点中，未发现多态现象，表明三种养殖鲍杂合子缺失严重，其主要原因可能在于哑基因、近交与自然选择。  相似文献   

中国鱼类学会第四次全国代表大会暨1993年学术年会在青岛召开     

朱鑫华  陈毅峰 《海洋与湖沼》1994,25(4):458-458

随着人类活动的频率不断加强，高额掠捕重要经济鱼类资源、环境污染直接威胁鱼类生态栖息地、人工增养殖培苗系统对天然鱼种遗传多样性的破坏等，已在相当程度上导致物种绝灭、传统鱼种资源衰退和种质资源的退化。因此，尽快查明我国鱼类物种多样性特征，研究持续利用重要鱼类资源和开展濒危鱼种的保护生物学研究，已成为当今鱼类学研究的优先主题，更需委我国鱼类学研究工作者的共同努力。本着这一热衷愿望，中国鱼类学会第四次全国代表大会暨1993年学术年会，于1993年10月25日-30日在青岛召开。这次会议得到了全国鱼类学界的积极响应，应…  相似文献