底质类型对热带海草海菖蒲种子萌发和幼苗生长的影响          下载免费PDF全文

刘松林  江志坚  吴云超  张霞  黄小平 《应用海洋学学报》2021,40(1):74-81

近几十年来,受到人类活动和气候变化的影响,全球海草床呈现退化趋势,海草床的恢复备受关注。其中,海草种子或幼苗移植由于其对供体海草床破坏和影响较小,并能保证海草的遗传多样性而备受重视。移植区的底质类型是决定海草种子或幼苗移植存活率的重要因子,然而,目前关于热带海草种子萌发和幼苗生长对不同底质类型响应的研究很有限。本研究以热带海草海菖蒲(Enhalus acoroides)种子为研究对象,利用室内模拟实验,分别设置细砂和砂砾底质的处理,探讨不同底质类型对海菖蒲种子萌发和幼苗生长的影响。研究发现,细砂组和砂砾组的萌发率和萌发历期的平均值分别96.3%、3.6 d和95.0%、3.4 d,两个处理组之间差异不显著。萌发后的细砂组海菖蒲幼苗的存活率为97.37%,而砂砾组的幼苗存活率仅为81.58%;另外,海菖蒲幼苗的叶片长度、根长度、叶片生长速率和根生长速率在细砂组均显著高于砂砾组。因此,沉积物粒径的差异对海草种子的萌发率和萌发时间没有显著性影响,但沉积物粒径增大会显著降低萌发后海菖蒲幼苗的存活率和生长速率。因此,未来开展海菖蒲种子或幼苗的野外移植,应选取沉积物粒径较小细砂质区域进行移植,可促进海菖蒲种子或幼苗移植的成活率,提高海菖蒲生态修复的成功率。  相似文献   

斑纹鲟凝集素(CFL)的初步研究 Ⅱ.CFL的分子组成及性质鉴定   总被引：3，自引：0，他引：3  

戴聪杰  王桂忠  李少菁  艾春香 《海洋学报》2006,28(3):98-102

采用生化方法对斑纹鲟(Charybdis feriatus)凝集素进行了初步研究.结果表明,斑纹鲟的血清中有动物红细胞和微生物细胞凝集活性的凝集素(Charybdis feriatuslectin,CFL),其活力表现依赖于Ca2+,并为多种糖所抑制;CFL对40℃以上的温度敏感,其活力在pH6.0~8.0内稳定.经巯基乙醇处理和未经巯基乙醇处理的CFL在SDS-PAGE上均为一条蛋白带,说明斑纹鲟凝集素不含二硫键,且只有一种亚基,分子量为40.8 kDa,CFL氨基酸组成分析表明其富含酸性氨基酸.  相似文献   

Effects of tributyltin at environmental levels on monooxygenase system of digestive gland in hard clam Meretrix meretrix     

HUANG Zhouying  CHEN Yixin  WANG Chonggang  ZHAO Yang  ZUO Zhenghong 《海洋学报(英文版)》2007,26(2):144-149

The effects on ethoxyresorufin O-deethylase （EROD）, NADPH-cytochrome c reductase and NADH cytochrorne b5 reductase activities of digestive gland in Meretrix meretrix exposed to tributyltin （TBT） at environmental levels （0.1,1.0,10.0 ng/dm as stannum concentration）,in experimental condition, were evaluated. The EROD, NADH cytochrorne b5 reductase activities were significantly inhibited after exposure to 10.0 ng/dm^3 TBT for 8 and 20 d, the NADPH cytochrorne c reductase activities were significantly inhibited after exposure to 0.1,1.0 and 10.0 ng/dm TBT for 8 d and to 1.0 and 10.0 ng/dm for 20 d, as compared with the matched control, while NADH cytochrorne b5 reductases and NADPH cytochrome c reductase activities were induced after exposure to 10.0 ng/dm^3 TBT for 2 d. The EROD activity in the 10 ng/dm^3 group,and the NADH cytochrome b5 reductases activities in 1.0 and 10.0 ng/dm groups, were significantly induced when transferred to clean recovery tanks for 7 d. The three enzymic activities in the clams exposed to TBT were recovered to the level corresponding to that of the control group after transfer to clean recovery tanks for 20 d. NADPH cytochrome c reductase activity in Meretrix meretrix seems to be more sensitive to exposure of TBT than that of the EROD and NADH cytochrome b5 reductases. The results suggest that induction and inhibition by TBT to the monooxygenase system enzymic activity in Meretrix meretrix would simultaneously exist. The enzymic activities were inhibited by exposure for a long time. The results suggest that inhibition of the monooxygenase system should be an indication of the exposure to environmentally relevant concentrations of TBT for a long time.  相似文献   

凤眼莲根对东海原甲藻生长的抑制作用及机制研究   总被引：8，自引：0，他引：8  

刘洁生  杨维东  陈芝兰 《热带海洋学报》2007,26(3):43-47

观察凤眼莲Eichhornia crassipes根粉末及丙酮提取物对东海原甲藻Prorocentrum donghaiense生长的影响,比较分析根系丙酮提取物中的化学成分以及不同成分抑藻效果,探讨凤眼莲根对藻类生长的抑制作用及其化学基础。结果显示,1.5g.L-1以上的凤眼莲根粉末可完全抑制东海原甲藻的生长。实际浓度0.019g.L-1的凤眼莲根丙酮提取物对东海原甲藻可产生50%的抑制率。N-苯基-2-萘胺浓度为1mg.L-1时,第6天对东海原甲藻的抑制率超过60%。浓度50μl.L-1时,亚油酸对东海原甲藻的抑制率超过80%。气相色谱-质谱联用(GC-MS)或高效液相色谱(HPLC)检测显示,凤眼莲根丙酮提取物中含有一定量的亚油酸和N-苯基-2-萘胺,同时还有大量的长链脂肪酸如十六酸、9-十六碳烯酸等。结果表明,凤眼莲根可显著抑制东海原甲藻的生长,N-苯基-2-萘胺、亚油酸可能是凤眼莲根抑藻的主要因子。  相似文献   

菲降解菌的降解特性及酶促反应动力学研究     

席宏波  杨琦  尚海涛  郝春博 《地学前缘》2008,15(6)

通过对取自MBR膜生物反应器中的活性污泥加入菲进行富集培养、驯化,分离、纯化出一株能以菲为唯一碳源和能源的短杆状革兰氏阴性菌J-1,细菌长2～5μm,宽1～3μm;研究了初始底物浓度、温度、pH对菌株J-1降解菲的影响,探讨了菌株J-1胞内酶对菲降解的底物抑制动力学。试验表明:菌株J-1在48h内能将不同浓度菲的水溶液中的菲完全降解;菲浓度增加,达到完全降解的时间延长。温度对细菌的降解能力影响较大,菌株J-1对菲降解的最佳温度为28℃。1.15mg·L-1的菲,28℃时48h内能完全降解,而相同时间内10℃时的降解率仅为36.65%。菌株J-1对pH的波动具有一定的适应性,pH在一定范围内(6.0～8.4)变化对菲降解的影响不大,降解反应的最佳pH为7.2。菌株J-1对菲的降解符合一级动力学反应方程。较高的底物浓度对酶促降解反应具有抑制作用,酶促反应的最大速率常数vm=1.17mg·L-1·h-1,米氏常数Km=61.70mg·L-1;底物抑制常数kS=49.60mg·L-1;最佳底物浓度[S]opt=55.32mg·L-1。  相似文献   

Seed germination of five Helianthemum species: Effect of temperature and presowing treatments     

F. Prez-García  M.E. Gonzlez-Benito 《Journal of Arid Environments》2006,65(4):688-693

The germination behaviour of five Helianthemum species (H. almeriense, H. appeninum, H. cinereum, H. hirtum, H. squamatum) has been studied under controlled conditions. Constant 15, 20 or 25 °C and alternating 25/15 °C temperature regimes and 16/8 h light/dark photoperiod conditions were used. Presowing treatments applied were manual scarification, boiling water, hot water, dry heat and sulphuric acid. Germination values recorded were final germination percentage and germination rate expressed as days to reach 50% of the final germination percentage (T₅₀). Incubation temperature had no significant effect on final germination percentage for untreated seeds of the five Helianthemum species. However, variation due to temperature was significant for scarified seeds, with the lowest germination percentage attained at 25 °C. In all Helianthemum species studied, the highest germination percentages were obtained by manual scarification of seeds. Germination rate of scarified seeds decreased as germination temperature increased. The different presowing treatments investigated allowed some germination in some species, but none were any better than manual scarification. The high germination among most species studied, following mechanical rupture of the seed coat, shows that the mechanism of dormancy lies in the seed coat. The physical dormancy caused by impermeable seed-coat appears to be the main reason of poor germination of untreated seeds of Helianthemum species studied.  相似文献   

刚毛柽柳种子萌发对盐分与干旱胁迫的响应     

孙红叶  李利  刘国军  张希明  李应罡 《干旱区地理》2007,30(3):14-419

通过对不同温度、盐分和干旱胁迫对刚毛柽柳种子萌发实验研究影响,结果表明:刚毛柽柳种子的适宜萌发温度范围为10~35℃,萌发率高达80%以上,且萌发迅速。不同浓度NaCl溶液对刚毛柽柳种子萌发有明显抑制作用,种子萌发率随NaCl溶液浓度的增大而下降,到0.7 mol/L时,萌发率仅为1.67%,种子发芽受到抑制。相同渗透势PEG-6 000溶液中的萌发率低于NaCl溶液,渗透势为-0.5 MPa是二者影响作用的分界点。渗透势为-1.8 MPa时,萌发率已急剧下降到0,其对种子萌发的抑制作用大于NaCl溶液。将在两溶液中处理10天的种子转移至蒸馏水后,随着NaCl溶液浓度的增大,萌发恢复率逐渐升高,0.7 mol/L时,萌发恢复率为60%;随着PEG-6 000溶液浓度增大,萌发恢复率先升高后降低,渗透势为-1.4 MPa时,种子萌发恢复率达到最大为67.5%,但两种处理下种子的最终萌发率(总萌发率)都低于在蒸馏水中的。  相似文献   

腐解9个月后杉木枯枝落叶化感物质对杉木的化感作用     

曹光球  刘学芝  林思祖  胡宗庆  王凌霄 《福建地理》2007,(2)

采用有机溶剂与蒸馏水混和浸提杉木枯枝落叶腐解9个月后化感物质,应用杉木种子发芽试验进行生物检测.结果表明:杉木枯枝落叶腐解9个月后,杉木枯枝落叶中的弱极性化感物质对绝对发芽率、绝对发芽势、胚根长、胚轴长及干物质量等指标表现为抑制作用,对鲜物质量则表现为轻微的促进作用;极性化感物质对杉木种子发芽各指标均等表现为抑制作用.腐解土中的弱极性化感物质对杉木种子发芽各指标均表现为抑制作用,极性化感物质对绝对发芽率、绝对发芽势、胚轴长及干物质量等表现为抑制作用,但对胚根长、鲜物质量表现为促进作用.  相似文献   

腐解9个月后杉木枯枝落叶化感物质对杉木的化感作用   总被引：5，自引：0，他引：5  

曹光球  刘学芝  林思祖  胡宗庆  王凌霄 《亚热带资源与环境学报》2007,2(2):15-20

采用有机溶剂与蒸馏水混和浸提杉木枯枝落叶腐解9个月后化感物质,应用杉木种子发芽试验进行生物检测.结果表明:杉木枯枝落叶腐解9个月后,杉木枯枝落叶中的弱极性化感物质对绝对发芽率、绝对发芽势、胚根长、胚轴长及干物质量等指标表现为抑制作用,对鲜物质量则表现为轻微的促进作用;极性化感物质对杉木种子发芽各指标均等表现为抑制作用.腐解土中的弱极性化感物质对杉木种子发芽各指标均表现为抑制作用,极性化感物质对绝对发芽率、绝对发芽势、胚轴长及干物质量等表现为抑制作用,但对胚根长、鲜物质量表现为促进作用.  相似文献   

The Influence of Oxalate on Fe-Catalyzed S(IV) Oxidation by Oxygen in Aqueous Solution     

A. Wolf  F. Deutsch  P. Hoffmann  H. M. Ortner 《Journal of Atmospheric Chemistry》2000,37(2):125-135

This study demonstrates that oxalate has a strong inhibiting effect onFe-catalyzed S(IV) oxidation by oxygen in aqueous solution. While thepseudo-first order rate constant of S(IV) oxidation was determined to be1.6 × 10³ M^-1 s^-1 in experimentswithout oxalate, the oxidation of S(IV) was totally inhibited at a molarconcentration ratio of iron:oxalate = 1:5 at an oxalate concentration of 4M. Under these conditions, the Fe(II)/Fe(III) ratio remained nearlyconstant during the observed reaction time. The determined rate constants wereindependent of the initial oxidation state of iron. However, with increasingconcentrations of oxalate, a longer induction period is observed forexperiments with iron initially in the Fe(II) oxidation state.  相似文献