Complete nuclear ribosomal DNA sequence analyses of the black-footed abalone Haliotis iris     

Zhansheng Guo  Leng Han 《新西兰海洋与淡水研究杂志》2018,52(2):232-246

The black-footed abalone Haliotis iris is an economically important shellfish species in New Zealand. We successfully amplified, sequenced and analysed the complete nuclear ribosomal DNA (nrDNA) of H. iris. The length of the nrDNA was determined to be around 9.6?kb and included, in order, small subunit ribosomal RNA (nrSSU, 1858bp), internal transcribed spacer (ITS, 749?bp), large subunit ribosomal RNA (nrLSU, 3412bp) and an intergenic spacer (IGS, 3560–3662?bp). The nrLSU genes were identical in two individuals, whereas the nrSSU and ITS regions existed at three and four base differences, respectively. The IGS was more variable than the other nrDNA regions. A phylogenetic tree was constructed based on the ITS sequence datasets, which revealed that Haliotidae has two major subclades, mainly distributed in the North Pacific, Europe and Australia. The complete nrDNA sequence will be useful for the classification, phylogeny and breeding of this shellfish.  相似文献   

Phaeocystis globosa与Phaeocystis antarctica叶绿体psbA基因的比较   总被引：3，自引：0，他引：3       下载免费PDF全文

杨泽民  章群  谢数涛  韩博平  吕颂辉  Hodgkiss 《海洋科学》2005,29(1):24-28

测定了2株球形棕囊藻Phaeocystis globosa P1、P2的psbA基因序列。发现得到的2个序列完全相同。以P2序列对比分析了P．globosa和P．antarctica的psbA基因在DNA序列、氮基酸序列和RNA二级结构上的差异性，发现2种棕囊藻psbA基因DNA序列和氨基酸序列非常保守，无插入／缺失，其核苷酸和氨基酸变异率分别为1．88％和1．13％。与核基因核苷酸的碱基替换不同，psbA基因核苷酸的碱基替换主要发生在密码子的第1位上。且不引起氨基酸的变化，引起氨基酸变化的碱基替换都发生在密码子的第2位和第3位上。在RNA二级结构上两序列的1～4茎环结构完全相同，表现出明显的棕囊藻属的特异性，其它结构区域差异较大。种间差异表现明显。由于psbA基因DNA序列和氨基酸序列非常保守，可能不适宜棕囊藻属的系统发育分析。但其RNA二级结构可能对于棕囊藻的分子分类有一定的参考价值。  相似文献   

军曹鱼的分子遗传特性研究   总被引：8，自引：1，他引：8  

刘楚吾  刘丽  王中铎  黎雨轩 《热带海洋学报》2005,24(1):77-85

采用随机扩增多态性DNA(RAPD)和线粒体DNA(mtDNA)的限制性片段长度多态性(RFLP)分析方法对广东湛江近海军曹鱼Rachycentron canadum的分子遗传学特征进行了研究。RAPD研究结果显示17个引物共检测到119个位点，其中多态位点比例P为80．85％，遗传相似系数S为0．7400，遗传距离D为0．2600，Nei基因多样性指数H为0．3009，Shannon信息指数I为0．4498。结果表明，军曹鱼的遗传多样性比较丰富。用19种识别5、6碱基的限制性内切酶对军曹鱼的mtDNA RFLP进行了分析研究，构建其mtDNA物理图谱；用引物5’-GTG ATC TGA AAA ACC ACC GTT G-3’和5’-AAT AGG AAG TAT CAT TGC GGT TTG ATG-3’扩增线粒体细胞色素b区段，得到稳定的850bp大小的特异性条带，用18种识别4-6碱基的限制性内切酶对扩增片段的限制性长度多态性进行分析，并测定其序列。  相似文献   

Phylogenetic analysis of Pectinidae(Bivalvia) based on the ribosomal DNA internal transcribed spacer region     

HUANG Xiaoting  BI Ke  HU Jingjie  HU Xiaoli  BAO Zhenmin 《海洋学报(英文版)》2007,26(6):83-90

The ribosomal DNA internal transcribed spacer (ITS) region is a useful genomic region for understanding evolutionary and genetic relationships. In the current study, the molecular phylogenetic analysis of Pectinidae (Mollusca: Bivalvia) was performed using the nucleotide sequences of the nuclear ITS region in nine species of this family. The sequences were obtained from the scallop species Argopecten irradians, Mizuhopecten yessoensis, Amusium pleuronectes and Mimachlamys nobilis, and compared with the published sequences of Aequipecten opercularis, Chlamys farreri, C. distorta, M. varia, Pecten maximus, and an outgroup species Perna viridis. The molecular phylogenetic tree was constructed by the neighbor-joining and maximum parsimony methods. Phylogenetic analysis based on ITS1, ITS2, or their combination always yielded trees of similar topology. The results support the morphological classifications of bivalve and are nearly consistent with classification of two subfamilies (Chlamydinae and Pectininae) formulated by Waller. However, A. irradians, together with A. opercularis made up of genera Amusium, evidences that they may belong to the subfamily Pectinidae. The data are incompatible with the conclusion of Waller who placed them in Chlamydinae by morphological characteristics. These results provide new insights into the evolutionary relationships among scallop species and contribute to the improvement of existing classification systems.  相似文献   

A genetic assessment of the taxonomic status of New Zealand mussels of the genus Xenostrobus Wilson, 1967     

Donald J. Colgan  Richard C. Willan  Lisa A. Kirkendale 《新西兰海洋与淡水研究杂志》2020,54(2):271-285

ABSTRACT

The genus Xenostrobus consists of small, marine and estuarine mussels that all appear similar externally. One of its estuarine species, Xenostrobus securis, with a native range in New Zealand and Australia, has become invasive in the Northern Hemisphere. No genetic data are available to determine if X. securis populations from the two countries are conspecific. Additionally, marine Xenostrobus from New Zealand have often been regarded as a species, X. neozelanicus, distinct fromthe marine Australian species X. pulex. We combined new DNA sequences with published data to assess the taxonomic status of New Zealand Xenostrobus. The data comprised 658 aligned bases of mitochondrial cytochrome c oxidase subunit I (COI) gene and 331 bases of nuclear histone H3. There was no evidence that X. securis populations from Australia and New Zealand are specifically distinct. Northern Hemisphere specimens of X. securis belonged to Australian, not New Zealand, clades in phylogenetic analyses of COI data, suggesting the former country as their more likely original source. The results confirm that X. neozelanicus and X. pulex are distinct species and for nomenclatural completeness for this taxonomic decision a lectotype is designated for Mytilus ater Zelebor in Dunker and Zelebor, 1866 DunkerW, ZeleborJ. 1866. Bericht über die von der Novara-expedition mitgebrachten Mollusken. Verhandlungen der Kaiserlich-Königlichen Zoologisch-Botanischen Gesellschaft in Wien. 16:909–916. [Google Scholar] [?=?Modiolus neozelanicus Iredale, 1915 IredaleT. 1915. A commentary on Suter’s ‘Manual of New Zealand Mollusca’. Transactions of the New Zealand Institute. 47:417–497. [Google Scholar]].  相似文献   

褐牙鲆(♀)、夏鲆(♂)及其杂交子一代线粒体16S rDNA序列遗传特性的初步研究   总被引：2，自引：0，他引：2  

徐晖  肖志忠  孔晓瑜  李军  董晓丽 《热带海洋学报》2007,26(5):60-63

以线粒体16S rRNA基因部分片段为代表研究雌褐牙鲆Paralichthys olivaceus、雄夏鲆P. dentatus及其杂交子一代间线粒体DNA的遗传特性。母本与父本序列差异较大,属种间差异,在590个位点中有28个变异位点,而且全部为简约信息位点。使用同一对引物扩增亲本与杂交子代,在杂交子代中未检测到父本的线粒体DNA类型,11个处于两种生长阶段的杂交样本仅检测到一种单倍型,而且与母本的一个单倍型为同一种,表明褐牙鲆与夏鲆杂交线粒体DNA遵循母系遗传规律。  相似文献   

Spring-spawning herring (Clupea harengus L.) in the southwestern Baltic Sea: do they form genetically distinct spawning waves?     

Hede Jorgensen  Hanne B.; Hansen  Michael Moller; Loeschcke  Volker 《ICES Journal of Marine Science》2005,62(6):1065-1075

  相似文献   

6个虾种基因组DNA多态性分析   总被引：2，自引：0，他引：2  

许玉德  孙晟 《海洋科学》2001,25(1):9-11

采用RAPD方法检测了罗氏沼虾（Macrobrachium rosenbergii)、绿须虾（Aristeus virilis)、长毛对虾（Penaeus penicillatus)、日本对虾（P.japonicus)、斑节对虾（P.monodon)和周氏新对虾（Metapenaeus joyneri)等6个虾种的基因组DNA的多态性。用20个随机引物扩增得到492个DNA片段，根据这些片段的共享度计算出遗传距离并构建系统树。所得结果从DNA水平上反映出虾类在科属种不同分类阶元亲缘关系的远近，并为虾类现行的分类系统提供了分子生物学依据。  相似文献   

The DNA de-methylating agent 5-azacytidine does not restore CYP1A induction in PCB resistant Newark Bay killifish (Fundulus heteroclitus)     

Xabier Arzuaga  Wanda Calcao  Adria Elskus 《Marine environmental research》2004,58(2-5):517

Newark Bay (NB) killifish (Fundulus heteroclitus) have been chronically exposed to environmental contaminants that activate the aryl hydrocarbon receptor (AHR) and are tolerant to toxic effects and CYP1A induction provoked by AHR ligands. Resistance to CYP1A induction could be due to an epigenetic mechanism such as DNA methylation. We measured in-ovo CYP1A catalytic activity (ethoxyresorufin-O-deethylase, EROD) in NB and reference site killifish embryos aqueously exposed to various concentrations of the de-methylating agent 5-azacytidine, 5-AC (5, 50 and 500 μ(micro)M) with or without 0.2 μ(micro)g/l of the CYP1A inducer 3,3^′,4,4^′,5 pentachlorobiphenyl (IUPAC PCB126). Neither PCB126 alone, nor PCB126 plus 5-AC, induced EROD above levels in vehicle treated Newark Bay fish. In reference site fish, the same PCB126 dose provoked a 7.4-fold EROD induction relative to controls. We conclude that Newark Bay killifish are resistant to CYP1A induction by co-planar PCBs during early embryological development and our data suggests that DNA methylation does not play a critical role in resistance to CYP1A induction in this model.  相似文献   

6-磷酸山梨醇脱氢酶(gutD)基因克隆、测序和表达     

陈启伟  刘广发  邱凤英 《台湾海峡》2004,23(3):314-317

本研究根据6-磷酸山梨醇脱氢酶基因(gutD)两端序列设计引物，以假单胞杆菌(Pseudonmonas XM)的总DNA为模板通过PCR的方法克隆gutD基因并进行序列分析，将克隆得到的gutD基因与原核表达载体pBV220连接并转化大肠杆菌(Escherichia coli)JM101后检测其蛋白表达及菌株生长耐盐性。  相似文献