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1.
阐述把维生素Ｂ１２生产菌添加到培养水中培养褶皱臂尾轮虫ＢｒａｃｈｉｏｎｕｓＰｌｉｃａｔｉｌｉｓ的买验。共１８株细菌分离于轮虫培养池，其中，有一株产维生素Ｂ１２的假单胞杆菌ＴＰ４对轮虫的生长繁殖有明显的促进作用。把ＴＰ４菌株培养后，加入到２Ｌ的烧杯和５００Ｌ的水槽中培养泰国Ｓ型轮虫时．在９ｄ（天）和６ｄ（天）中，轮虫密度从１２４～１３９和２４２～２８８个体／ｍｌ增殖到４，４１７～５，５４０和１，０１７～１，２５４个体／ｍｌ，分别比对照组增加了４～６及２～３倍。 相似文献
2.
本实验旨在探究维生素E对半滑舌鳎垂体组织中生长激素基因表达的影响。作者在每千克等氮等能的基础饲料中分别添加0、400和1 600mg的DL-α-生育酚乙酸酯(维生素E)投喂半滑舌鳎(Cynoglossus semilaevis)成鱼(464±2.6) g,进行为期8周的养殖实验;另外,在L-15培养基中添加0、18和54μmol/L的维生素E,对半滑舌鳎成鱼(464±2.6) g的垂体细胞进行为期3 d的体外原代培养实验。分别取垂体组织和原代细胞,通过荧光实时定量PCR分析其gh mRNA的相对表达量。实验结果表明:垂体组织中gh mRNA的相对表达量随着饲料中维生素E含量的增加而呈现先升高后下降的变化趋势,在400 mg/kg组时显著高于其他各组(P0.05);随着细胞培养液中维生素E浓度的升高, gh mRNA的相对表达量显著增加(P0.05)。由此可见,适宜浓度的维生素E能够促进半滑舌鳎垂体组织中生长激素基因的表达。 相似文献
3.
A series of enclosed ecosystem experiments were conducted in a land-based tank near the seaside of West Xiamen Harbor. The
results of experiments conducted in different seasons and years showed a repeatable phytoplankton succession. In this relatively
stable ecosystem with added nutrients and trace metals, diatoms dominated initially, dinoflagellates dominated in the later
stage, and dinoflagellate red tides eventually occurred. Vitamin B12 enrichment may speed up this succession process. Stirring the water column could stop this process. Soluble Mn at a level
of 3–4 μg/L in seawater, which also is the existing concentration of soluble Mn in Xiamen Harbor seawater, is sufficient for
the multiplication of algae and occurrence of red tide. The present study showed that excessive soluble Mn in Xiamen Harbor
cannot cause red tide, and that Fe was one of the important factors causing diatiom red tide in this present study.
Project 39570145 supported by NSFC. 相似文献
4.
The disease resistance and humoral immunomodulatory effects of vitamin C administered orally to grouper,Epinephelus awoara maintained on a frozen fish diet supplemented with vitamin C at 500, 1000, 1500 and 2000 mg/kg were investigated. After 20
weeks, the growth rates of the groups with high level of vitamin C apparently increased. The untreated fish had symptoms of
vitamin C deficiency. The endogenous liver tissue vitamin C levels were found to reflect well the dietary treatments. After
intraperitoneal injection or bath challenge with a virulent strain ofVibrio vulnificus, fish fed with high level vitamin C showed significantly higher survival rate compared with the normal control group. Vaccination
with formalin inactivatedV. vulnificus significantly enhanced the specific antibody production in fish treated with vitamin C, and completely protected from strong
bacterial challenge the groups fed on fish with vitamin C 1500 and 2000 mg/kg diet.
Project 39500113 supported by NSFC and also by Guangdong Science Research Foundation (Grant No. 950711). 相似文献
5.
维生素D(VD)是一类具有抗佝偻病作用的类固醇激素,其中维生素D3(VD3)是VD在动物体内的主要存在形式。作为生命活动必不可少的一类物质,VD发挥着广泛且重要的生理学作用。VD在动物体内的代谢过程及相关的代谢酶具有较高的进化保守性,同时也受到多种因素的调控影响。由于鱼类在生活环境方面与陆生脊椎动物存在显著差异,VD在鱼类中的合成代谢及生理功能与高等动物也存在一定的区别。本文主要概述了鱼类VD3的主要来源以及需求量的研究,总结了近年来鱼类维生素D受体(VDR)、VD3代谢调控及其生理功能的研究进展,比较了鱼类和陆生高等动物在VD来源、代谢以及VDR等方面的差异,并对水生动物VD的未来研究方向进行了展望。 相似文献
6.
采用批量培育法研究了在面包酵母悬浮液中添加不同浓度的脂溶性维生素对褶皱臂尾轮虫（Ｂｒａｃｂｉｏｎｕｓｐｌｉｃａｔｉｌｉｓ）增殖率的影响。结果表明：面包酵母对褶皱臂尾轮虫的生长繁殖是有营养缺陷的。在面包酵母悬浮液中．当添加维生素Ａ为０．０２～５．００μｇ／ｍｌ时，随着添加浓度的增大，轮虫的种群增殖率Ｒ值也增大。以添加５．００μｇ／ｍｌ为最佳，第４天的Ｒ值达０．７５，是对照组的５．６倍。添加维生素Ｄ０．００５～０．２０μｇ／ｍｌ对轮虫的增殖有一定促进作用，以添加０．０５μｇ／ｍｌ为最佳，第７天的Ｒ值为０．４０７，是对照组的２．７倍。添加维生素Ｅ０．００１～０．０１μｇ／ｍｌ和添加维生素Ｋ０．０１～０．１０μｇ／ｍｌ时，第４天的Ｒ值与对照组无差异。 相似文献
7.
中国对虾（Penaeus chinensis）维生素营养的研究──Ⅴ．中国对虾维生素C营养 总被引：1，自引：0，他引：1
提出以稳定态的ＬＡＰＰ代替一般维生素Ｃ添加在配合饲料中饲喂对虾，指出：在１００ｇ饲料中含ＬＡＰＰ４００ｍｇ时，其体长增长率和体重增重率最高，存活率最佳．饲料中ＬＡＰＰ浓度大小影响对虾的蜕壳频率和蜕壳周期，当饲料中ＬＡＰＰ缺乏时，对虾的蜕壳频率降低，蜕壳周期延长；过量的ＬＡＰＰ也会使对虾的蜕壳频率相对降低，但比缺乏或不足的要高。维生素Ｃ在对虾肝胰脏中的积累以ＬＡＰＰ含量４００ｍｇ／１００ｇ饲料组为最高。 相似文献
8.
饲料中维生素B6对皱纹盘鲍幼鲍蛋白质代谢的影响 总被引：4，自引：0，他引：4
以皱纹盘鲍(Haliotis discus hannaiIno)幼鲍为研究对象,探讨维生素B6对其蛋白质代谢的影响。设计了3种维生素B6不同含量(0,40,4 000 mg/kg)的半精制饲料,在循环水养殖系统中养殖幼鲍240 d。结果表明,皱纹盘鲍内脏团和肌肉中磷酸吡哆醛(PLP)的含量随饲料中维生素B6的添加而显著升高(P<0.05)。饲料中不同维生素B6水平对肌肉组织中蛋白含量没有显著影响(P>0.05),而显著升高内脏团中蛋白质的含量(P<0.05)。内脏团和肌肉中的谷丙转氨酶(ALT)和谷草转氨酶(AST)活力随着饲料中维生素B6添加量的升高而显著增强,分别在维生素B6的添加量为4 000 mg/kg时取得最大值(P<0.05)。内脏团中所检测的各种氨基酸的含量与饲料中维生素B6添加量没有显著影响(P>0.05)。然而,肌肉中的天冬氨酸(Asp)、苏氨酸(Thr)、丝氨酸(Ser)、牛磺酸(Tau)的含量随着饲料中维生素B6添加量的升高而显著增加,并且分别在维生素B6添加量为4 000 mg/kg时取得最大值(P<0.05)。肌肉中甘氨酸(Gly)、丙氨酸(Ala)、缬氨酸(Val)、蛋氨酸(Met)的含量在两个维生素B6添加组(40 mg/kg和4 000 mg/kg)之间没有显著差异(P>0.05),但均显著高于维生素B6缺乏组(0 mg/kg)的值(P<0.05)。 相似文献
9.
周智翔 《热带气象学报(英文版)》2009,15(1):1-12
On the basis of a comprehensive literature review and data analysis of global influenza surveillance, a transmission theory based numerical model is developed to understand the causative factors of influenza seasonality and the biodynamical mechanisms of seasonal flu. The model is applied to simulate the seasonality and weekly activity of influenza in different areas across all continents and climate zones around the world. Model solution and the good matches between model output and actual influenza indexes affirm that influenza activity is highly auto-correlative and relies on determinants of a broad spectrum. Internal dynamic resonance; variations of meteorological elements (solar radiation, precipitation and dewpoint); socio-behavioral influences and herd immunity to circulating strains prove to be the critical explanatory factors of the seasonality and weekly activity of influenza. In all climate regions, influenza activity is proportional to the exponential of the number of days with precipitation and to the negative exponential of quarter power of sunny hours. Influenza activity is a negative exponential function of dewpoint in temperate and arctic regions and an exponential function of the absolute deviation of dewpoint from its annual mean in the tropics. Epidemics of seasonal influenza could be deemed as the consequence of the dynamic resonance and interactions of determinants. Early interventions (such as opportune vaccination, prompt social distancing, and maintaining incidence well below a baseline) are key to the control and prevention of seasonal influenza. Moderate amount of sunlight exposure or Vitamin D supplementation during rainy and short-day photoperiod seasons, more outdoor activities, and appropriate indoor dewpoint deserve great attention in influenza prevention. To a considerable degree, the study reveals the mechanism of influenza seasonality, demonstrating a potential for influenza activity projection. The concept and algorithm can be explored for further applications. 相似文献
10.
为探讨复合维生素B(叶酸、B_6和B_(12))能否抑制PM_(2.5)对大鼠的急性肺损伤作用,本研究将56只SD大鼠随机分成对照组、不同剂量(0. 4、2. 0、10. 0 mg/m L) PM_(2.5)染毒组和相应的复合维生素B干预组(0. 02 mg/m L叶酸、1 mg/m L维生素B6、0. 002 5 mg/m L维生素B12),取肺组织进行HE染色观察组织病理学形态变化并进行病理学评分;收集支气管肺泡灌洗液(BALF)测定总蛋白(TP)、乳酸脱氢酶(LDH)、碱性磷酸酶(AKP)、酸性磷酸酶(ACP)、丙二醛(MDA)、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)的含量。结果显示,PM_(2.5)染毒能对大鼠肺组织造成不同程度的病理性损伤,复合维生素B能减缓此损伤;与对照组相比,低、中、高剂量的PM_(2.5)染毒大鼠可引起BALF中LDH、AKP、ACP、TP和MDA含量显著升高(P 0. 05),而SOD和CAT活性显著降低(P 0. 05),且TP和MDA含量随染毒剂量的增加而升高(P 0. 05),CAT活性随染毒剂量的增加而降低; 3个染毒剂量的复合维生素B干预组LDH、TP和MDA含量显著降低(P 0. 05),而SOD和CAT活性显著升高(P 0. 05); PM_(2.5)低、高剂量+复合维生素B组BALF中ACP含量显著降低(P 0. 05); PM_(2.5)中剂量+复合维生素B组BALF中ACP含量无显著改变;仅有PM_(2.5)低剂量+复合维生素B组AKP含量降低(P 0. 05),其余干预组AKP含量无显著变化。因此认为,PM_(2.5)降尘能打破机体的氧化-抗氧化平衡,造成大鼠急性肺损伤,而复合维生素B能减缓此损伤,对机体起到保护作用。 相似文献