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CHAN Zhuhua CHEN Xinglin HOU Yanping GAO Boliang ZHAO Chungui YANG Suping ZENG Runying 《海洋学报(英文版)》2018,(2)
Agarases are hydrolytic enzymes that act on the hydrolysis of agar and have a broad range of applications in food,cosmetics and pharmaceutical industries. In this study, a glycerol feeding strategy based on induction mode optimization for high cell density and β-agarase production was established, which could effectively control acetate yield. First, exponential feeding strategy of glycerol with different overall specific growth rates(μ) was applied in the pre-induction phase. The results showed that the low μ(μ=0.2) was suggested to be the optimal for cell growth and β-agarase production. Second, the effects of induction temperature and the inducer concentration on cell growth and β-agarase production were investigated in the post-induction phase. When induced by isopropyl-β-d-thiogalactoside(IPTG), the strategy of 0.8 mmol/L IPTG induction at 20℃ was found to be optimal for β-agarase production. When cultivation was induced by continuous lactose feeding strategy of 1.0 g/(L·h), the β-agarase activity reached 112.5 U/mL, which represented the highest β-agarase production to date.Furthermore, the β-agarase was capable of degrading G. lemaneiformis powder directly to produce neoagarooligosaccharide, and the hydrolysates were neoagarotetraose(NA4) and neoagarohexaose(NA6). The overall research may be useful for the industrial production and application of β-agarase. 相似文献
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A remarkable invariance in the ratio of 1,3-dimethylcarbazole （DMC） to 1,6-DMC was discovered in crude oils from the Pearl River Mouth Basin, South China Sea. The remarkably invariant ratio is kept at a constant of about unity regardless of their concentrations, sources or maturities for the sampies. In combination with the molecular structures of 1,3- and 1,6-DMCs, the invariance might indicate that the nitrogen compounds share a common precursor with a skeleton of 1-methylcarbazole and are formed through methylation at C3 and C6 with an essentially identical rate. 相似文献
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本文用1951—1984年的资料对500百帕、65°N以北的高度场作自然正交展开,用模糊聚类对典型场归类。指出,极涡月平均场有三种形态,1月份平均场形态较特殊;大尺度距平场也有三种形态,4月份距平场形态较特殊。此外,研究了典型场的时间权重系数与厄尔尼诺、南方涛动的关系。指出,厄尔尼诺现象发生的当年4月、10月极地大尺度距平场有明显的异常特征。这种极地环流异常已明显影响到我国4月份、10月份的气温场。因而,可能是厄尔尼诺年我国气温4月偏低、10月偏高的一个重要原因。 相似文献
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2013年夏季湖南严重高温干旱及其大气环流异常 总被引：2，自引：0，他引：2
利用湖南省97个台站降水、气温资料和NECP/NCAR再分析资料以及NOAA卫星观测的OLR场资料,系统分析了2013年6月30日至8月14日湖南持续高温干旱的时空分布及同期大气环流异常特征。结果表明：2013年夏季是湖南1951年以来降水最少、高温干旱程度最严重的一年。西太平洋副热带高压强度偏强、西伸脊点偏西,湖南处在副高控制下,盛行下沉气流是发生持续高温干旱的直接原因;西太平洋副高内增强的下沉气流,致使多数县市高温日数和极端高温突破历史同期最高记录。位于菲律宾附近的OLR低值带中心偏西,致使副热带地区下沉区偏西,进而造成西太平洋副高偏强偏西。此外,湖南地区上空为水汽输送异常辐散中心,这在一定程度上促使了干旱的发生发展。 相似文献
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基于湖南汛期区域持续性暴雨典型环流分型,利用1961—2016年NCEP/NCAR再分析资料和异常度方法对湖南6月区域持续性暴雨环流型进行客观识别,并结合动力和水汽输送条件,确定湖南6月区域持续性暴雨强信号并客观量化表征,建立湖南6月区域持续性暴雨预报定量化概念模型。结果表明:43次历史区域持续性暴雨过程的回报准确率达到81%,2017—2018年3次区域持续暴雨过程试报准确率为2/3,说明该概念模型有一定预报能力,能为湖南暴雨预报业务服务提供技术支持。将该概念模型与各类模式预报产品相结合,还可开展区域持续性暴雨的中期和延伸期预报。 相似文献
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As a major aldehyde pollutant widely existing in industry and our daily life, acetaldehyde is more and more harmful to human health. As characteristic habitat niche, bacteria from deep sea environments are abundant and distinctive in heredity, physiology and ecological functions. Thus, the development of acetaldehyde-degrading bacteria from deep sea provides a new method to harness acetaldehyde pollutant. Firstly, in this study,acetaldehyde-degrading bacteria in the deep sea water of the West Pacific Ocean were enriched in situ and in the laboratory respectively, and then the diversity of uncultured bacteria was studied by using 16 S r RNA genes. Then acetaldehyde-degrading strains were isolated from two samples, including enrichment in situ and enrichment in laboratory samples of deep sea water from the West Pacific Ocean using acetaldehyde as the sole carbon source,and then the ability of acetaldehyde degradation was detected. Our results showed that the main uncultured bacteria of two samples with different enrichment approaches were similar, including Proteobacteria,Actinobacteria, Firmicutes, Cyanobacteria, but the structure of bacterial community were significant different.Four subgroups, α, γ, δ and ε, were found in Proteobacteria group. The γ-Proteobacteria was dominant(63.5%clones in laboratory enriched sample, 75% clones in situ enriched sample). The species belonged to γ-Proteobacteria and their proportion was nearly identical between the two enrichment samples, and Vibrio was the predominant genus(45% in laboratory enriched sample, 48.5% in situ enriched sample), followed by Halomonas(9% in situ enriched sample) and Streptococcus(6% in laboratory enriched sample). A total of 12 acetaldehyde-degrading strains were isolated from the two samples, which belonged to Vibrio, Halomonas,Pseudoalteromonas, Pseudomonas and Bacillus of γ-Proteobacteria. Strains ACH-L-5, ACH-L-8 and ACH-S-12,belonging to Vibrio and Halomonas, have strong ability of acetaldehyde degradation, which could tolerate 1.5 g/L acetaldehyde and degrade 350 mg/L acetaldehyde within 24 hours. Our results indicated that bacteria of γ-Proteobacteria may play an important role in carbon cycle of deep sea environments, especial the bacteria belonging to Vibrio and Halomonas and these strains was suggested for their potentials in government of aldehyde pollutants. 相似文献
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湖南高温日时空分布特征及高温日数气候预测 总被引：1，自引：0，他引：1
利用1961—2013年湖南74个气象观测站逐日高温观测资料,建立了较完整的湖南高温日时间序列,分析了湖南高温日时空分布及变化趋势。结果表明,湖南高温日呈增加趋势,每10年增加2天左右,湘北高温日增加趋势显著;高温最长持续日数总体上湘南大部呈弱的减少趋势,每10年减少0.5天左右,湘中及湘北则呈较强的增加趋势,每10年增加1天左右。20世纪90年代以来,湖南高温天气显著增多、增强。利用最优子集回归构建气候预测模型,预测湖南高温平均出现日数,预测效果较好,可在业务中应用。 相似文献
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Bangjun CAO Shihua LYU Yu ZHANG Xianyu YANG Boliang LI Liang YUAN Meilin LI 《Journal of Meteorological Research》2022,(2):311-325
Using the cloud radar, ground observations, and ECMWF Reanalysis v5 (ERA5) data, we investigate the factors in-fluencing nighttime precipitation during summer i... 相似文献