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中国对虾卵细胞中病毒的初步研究 总被引:6,自引:0,他引:6
对中国对虾卵巢和受精卵细胞进行了电子显向镜超微结构观察,发现卵巢内有球状病毒粒子,大小为80nm左右,受精卵细胞内有传染性皮下和造血组织坏死病毒(IHHNV)感染,其严重影响对虾受精卵的发育,从获得的结果来看,中国对虾病毒(IHHNV)垂直传播的可能性极大。 相似文献
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Lymphocystis disease causes serious economic losses in the fish farming industry. The causative agent of the disease is Lymphocystis disease virus China (LCDV-cn), which has a wide range of hosts. Based on competitive quantitative PCR technology, we established a method to quantify the LCDV-cn in tissue. Results demonstrate that the average amount of LCDV-cn in the peripheral blood of infected flounder with evident tumors is about 106virions/ml while the average amount in those flounder with no evident tumor but cultured with the flounder with evident tumor is about 104virions/ml. No virus was found in the negative samples of flounder. 相似文献
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80年代以来 ,肝胰脏细小病毒病在养殖中国对虾中广泛流行并造成了一定的损失。作者采用免疫金银染色 (immunogold silver staining,IGSS)技术 ,对患病对虾肝胰脏中的细小病毒(Hepatopancreatic parvovirus,HPV)进行了免疫组织化学的研究。结果表明 ,经 IGSS染色的病虾肝胰脏上皮细胞核内出现了黑色的、圆形或椭圆形、大小为 3~ 15μm的结构。由于该结构与 H- E染色切片上出现的肝胰脏细小病毒包涵体在发生部位、形态和大小上皆一致 ,故而认为该结构为HPV包涵体。证明 ,免疫金银染色法是一种可应用于中国对虾肝胰脏细小病毒病诊断的有效方法。 相似文献
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中国台湾地区及世界部分主要海水养殖国家养殖疾病综述 总被引:2,自引:0,他引:2
本文综述了中国台湾地区及世界部分主要海水养殖国家和地区的水产养殖病害情况,对鱼虾贝类的病毒性疾病,细菌怀疾病及其他病症的流行情况,防治方法,病原特征,宿主范围和组织病理学特征等进行了较详细的介绍。本综述对我国的海水养殖业和水产病害研究将有重要的指导作用。 相似文献
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This pathogenic study shows that the viral diseases of Chinese prawns (Penaeus chinensis. O'sbeck)is due to three kinds of viruses: epithelium envelope baculovirus of Penaeus chinensis (EEBV-PC, de-tected by the authors in 1993), infections hypodermal and hematopoietic necrosis virus, andhepatopancreatic parvo-like virus, and that the first two viruses seem to be the main pathogens of theepidemic in the northern regions in 1993. 相似文献
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This paper describes a rapid procedure for diagnosis of penaeid viral disease using the immune serological method. The SPA (Staphylococcal Protein A) coagglutination test in early diagnosis of penaeid viral disease has yielded satisfactory results.This is the first time SPA is used in China for diagnoses of viral diseases of marine invertebrates, especially penaeid shrimp.The SPA early diagnostic method is characterized by accuracy, rapidity, simplicity and convenience, low cost, high specificity, strong sensitivity and micro-detectability, and easy dissemination and adaptability in prawn farms. 相似文献
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初期成活率是对虾养殖的第一个关键问题,如果初期虾池环境不良,虾苗会大量死亡。本文探索了优化虾苗投放环境的方法并提出了营养盐的合理指标。研究结果表明,良好的虾苗投放生态环境对于整个养成期十分重要。1材料与方法本试验在河北省唐海县十里海养殖场进行。试验面积13000亩,试验时间是1990~1991年,设对照池3000亩,试验对象为养殖的中国对虾。探索适当的肥水方法、监测浮游生物量、水质、虾苗成活率和旬生长速度等,推定提高初期成活率的虾池环境指标。2结果与讨论1990年春,虾苗入地后一个月生长十分缓慢… 相似文献
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