Adhesion mechanisms of Vibrio fluvialis to skin mucus of Epinephelus awoara     

鄢庆枇  赵敏慧  王晓露  邹文政  陈昌生 《中国海洋湖沼学报》2010,28(2):260-266

Vibrio fluvialis incubated in trypticase soy broth (TSB) showed stronger adhesion to the skin mucus of Epinephelus awoara than V. fluvialis grown on trypticase soy agar (TSA), and this bacterial adhesion was assessed in terms of saturation kinetics. Treating bacteria with antibody against O-antigens resulted in significantly reduced bacterial adhesion. In the early growth stage, the adhering bacteria numbers increased with incubation time, peaked at 24 h, and then dropped sharply. Prior heat treatment of the mucus at various temperatures resulted in different effects on bacterial adhesion. No remarkable effect on bacterial adhesion was detected after treating mucus with protease K and trypsin, whereas periodic acid treatment significantly enhanced bacterial adhesion. Extracts of V. fluvialis surface structures at high concentrations significantly inhibited bacterial adhesion, while relatively low concentrations enhanced adhesion. V. fluvialis had strong affinity for a high molecular weight mucus component. These results indicate that V. fluvialis adhered strongly to E. awoara mucus by specific adhesion and that this adhesion was influenced by many factors, including bacterial conditions, mucus, and the environment.  相似文献   

大黄鱼（Pseudosciaena crocea）一种新型抗菌肽的纯化及其生物学特性研究          下载免费PDF全文

纪勇  王晓露  苏永全  覃映雪  邹文政  邢颜丽  徐晓津  鄢庆枇 《海洋与湖沼》2011,42(3):448-454

以致病性溶藻弧菌为指示菌,通过酸粗提、固相萃取、Sephadex G-25柱凝胶层析和RP—HPLC等分离纯化技术,从大黄鱼胃肠组织中分离纯化抗菌肽。结果表明,大黄鱼肠道组织中存在着多种抗菌物质,并最终得到一种高纯度的新型抗菌肽AMP-W3。AMP—W3由10种氨基酸组成,其中天冬氨酸、谷氨酸和甘氨酸的含量较高。AMP...  相似文献   

绿色荧光蛋白标记的病原性河流弧菌在青石斑肠道的黏附定植研究     

覃映雪  赵敏慧  苏永全  王军  张小东  王晓露  鄢庆枇 《海洋学报》2012,34(6):170-176

通过灌胃将绿色荧光蛋白标记的病原性河流弧菌导入青石斑鱼消化道内,追踪标记菌在青石斑肠道中的黏附和定植及对肠道消化酶活性的影响。结果表明,青石斑前肠和中肠在河流弧菌灌胃12 h即可检出,前肠在24 h达到峰值,大约为7.1×105 cfu/g,中肠则在36 h达到峰值,约为5.39×105 cfu/g,而后肠则是在24 h才开始检出,且检出时即达到峰值,但仅为2.44×104 cfu/g,说明青石斑鱼的前肠和中肠都是河流弧菌的主要黏附部位。灌胃96 h后前肠和中肠仍有标记菌存在,但在后肠已检测不到标记菌,反映出标记菌已在前肠和中肠定植下来,但没有在后肠定植。此外,标记河流弧菌的定植能够显著降低前肠蛋白酶活性和淀粉酶活性以及后肠脂肪酶活性。研究结果说明,病原性河流弧菌可通过黏附定植于鱼类肠道影响鱼类肠道消化酶活力,这可能是病原性河流弧菌引发鱼类病害的原因之一。  相似文献   

Adhesion mechanisms of Vibrio fluvialis to skin mucus of Epinephelus awoara     

鄢庆枇  赵敏慧  王晓露  邹文政  陈昌生 《中国海洋湖沼学报》2010,(2)

Vibrio fluvialis incubated in trypticase soy broth(TSB)showed stronger adhesion to the skin mucus of Epinephelus awoara than V.fluvialis grown on trypticase soy agar(TSA),and this bacterial adhesion was assessed in terms of saturation kinetics.Treating bacteria with antibody against O-antigens resulted in significantly reduced bacterial adhesion.In the early growth stage,the adhering bacteria numbers increased with incubation time,peaked at 24 h,and then dropped sharply.Prior heat treatment of the mucus at various temperatures resulted in different effects on bacterial adhesion.No remarkable effect on bacterial adhesion was detected after treating mucus with protease K and trypsin,whereas periodic acid treatment significantly enhanced bacterial adhesion.Extracts of V.fluvialis surface structures at high concentrations significantly inhibited bacterial adhesion,while relatively low concentrations enhanced adhesion.V.fluvialis had strong affinity for a high molecular weight mucus component.These results indicate that V.fluvialis adhered strongly to E.awoara mucus by specific adhesion and that this adhesion was influenced by many factors,including bacterial conditions,mucus,and the environment.  相似文献