Development of two novel CYP-antibodies and their use in a PCB exposure experiment with Mytilus edulis     

H. Jonsson  K. Viken Sandnes  D. Schiedek  R. Schneider  B. E. Grsvik  A. Goksyr 《Marine environmental research》2004,58(2-5):655

In an attempt to learn more about the cytochrome P450 (CYP) system of mussels, we used protein databases and alignment software to extract highly conserved CYP sequences. From these alignments synthetic peptides were produced and used for rabbit immunisation, which yielded polyclonal antibodies against the CYP families 2 and 4. The antibodies were evaluated with Western Blot and ELISA assays, using digestive gland microsomal samples from the mussel Mytilus edulis. Western Blots revealed immunoreactions for both antibodies. The anti-CYP2 sequence rendered one major immunopositive protein of ≈49 kDa size, and weak signals for proteins of ≈41 and 56 kDa size. The anti-CYP4 sequence rendered two major bands of ≈56 and 59 kDa size, and also a weak immunoreaction with a protein of ≈43 kDa size. ELISA rendered only weak signals even with a 1:50 dilution of IgG-purified serum. A 10-day exposure to Aroclor 1254 did not appear to affect any of the immunopositive proteins, while total PCBs in soft bodies increased from 14–40 ng/g DW in controls to 373–638 ng/g DW in exposed mussels.  相似文献   

Development of two novel CYP-antibodies and their use in a PCB exposure experiment with Mytilus edulis     

Jonsson H  Sandnes KV  Schiedek D  Schneider R  Grøsvik BE  Goksøyr A 《Marine environmental research》2004,58(2-5):655-658

In an attempt to learn more about the cytochrome P450 (CYP) system of mussels, we used protein databases and alignment software to extract highly conserved CYP sequences. From these alignments synthetic peptides were produced and used for rabbit immunisation, which yielded polyclonal antibodies against the CYP families 2 and 4. The antibodies were evaluated with Western Blot and ELISA assays, using digestive gland microsomal samples from the mussel Mytilus edulis. Western Blots revealed immunoreactions for both antibodies. The anti-CYP2 sequence rendered one major immunopositive protein of approximately 49 kDa size, and weak signals for proteins of approximately 41 and 56 kDa size. The anti-CYP4 sequence rendered two major bands of approximately 56 and 59 kDa size, and also a weak immunoreaction with a protein of approximately 43 kDa size. ELISA rendered only weak signals even with a 1:50 dilution of IgG-purified serum. A 10-day exposure to Aroclor 1254 did not appear to affect any of the immunopositive proteins, while total PCBs in soft bodies increased from 14-40 ng/g DW in controls to 373-638 ng/g DW in exposed mussels.  相似文献   

The flow of Atlantic water to the North Icelandic Shelf and its relation to the drift of cod larvae   总被引：1，自引：0，他引：1  

Jonsson  Steingrimur; Valdimarsson  Hethinn 《ICES Journal of Marine Science》2005,62(7):1350-1359

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Corrigendum to “Seasonal food web structures and sympagic-pelagic coupling in the European Arctic revealed by stable isotopes and a two-source food web model” [Progress in Oceanography 71 (2006), 59-87]     

Janne E. Søreide  Haakon Hop  Stig Falk-Petersen 《Progress in Oceanography》2007,73(1):96-98

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A note on wave action conservation in a dissipative current wave motion     

J.B. Christoffersen  I.G. Jonsson 《Applied Ocean Research》1980,2(4):179-182

We consider steady, slowly varying water waves propagating on a steady current over a gently sloping bed, so-called current depth refraction. All expressions are correct to second order in wave amplitude. Formulating the energy equation for the fluctuating motion in terms of wave action (wave energy divided by intrinsic angular frequency) results in an expression, where the dissipative term is strikingly similar to wave action itself. It is simply the ‘extra’ dissipation (per unit area) caused by the fluctuating motion (i.e. total dissipation minus the effect of current acting on total mean bed shear stress) divided by the intrinsic angular frequency. We call it ‘wave action dissipation’. An inconsistency in Phillips' (1977) book is pointed out. A new formula for the calculation of wave amplitudes along rays is set forth.  相似文献   

Single frequency processing of Ørsted GPS radio occultation measurements     

Georg Bergeton Larsen  Stig Syndergaard  Per Høeg  Martin Bjært Sørensen 《GPS Solutions》2005,9(2):144-155

The Global Positioning System (GPS) radio occultation measurements obtained using the TurboRogue GPS receiver on the Danish satellite Ørsted have been processed using the single frequency method. Atmospheric profiles of refractivity and temperature are derived and validated against numerical weather prediction data from the European Centre for Medium-Range Weather Forecast (ECMWF). Results from the Ørsted GPS measurement campaign in February 2000 indicate that the single frequency method can provide retrievals with accuracy comparable to that of using two frequencies. From comparisons between measured dry temperature profiles and corresponding dry temperature profiles derived from ECMWF analysis fields, we find a mean difference of less than 0.5 K and a standard deviation of 2–4 K between 500 and 30 hPa in height. Above 30 hPa the impact of the ionosphere becomes more dominant and more difficult to eliminate using the single frequency method, and the results show degraded accuracy when compared to previous analysis results of occultation data from other missions using the dual frequency method. At latitudes less than 40° (denoted low latitudes), the standard deviation is generally smaller than at latitudes higher than 40° (denoted high latitudes). A small temperature bias is observed centered at 200 hPa for low latitudes and at 300 hPa for high latitudes. This indicates that the ECMWF analyses do not adequately resolve the tropopause temperature minimum. In the lowest part of the troposphere an observed warm bias is thought to be due to erroneous tracking of the GPS signal in cases of atmospheric multipath propagation.  相似文献   

电离层水平不均匀性对无线电掩星资料反演的影响   总被引：2，自引：4，他引：2       下载免费PDF全文

张训械  曾桢  胡雄  Per Hoeg  G.B.Larsen  B.S.Martin  C.R.Jakob 《地球物理学报》2002,45(Z1):24-29

电离层水平不均匀结构是引起掩星资料反演的主要误差源.本文模拟不均匀结构对无线电掩星资料反演的影响.首先利用3-D全球射线追踪技术进行了模拟.结果指出:电离层电子密度20%的变化可能引起GPS的时延增加约12m.其次,将90%的掩星时延对应的路径长度,定义为电离层掩星测量的水平分辨率,其值为1200-2000 km.最后,计算了不同纬度电离层当地正午、子夜和日出时的电离层水平不对称指数.它表明:低纬夏季日出时,不对称指数达-78%,反演结果可能有较大误差.  相似文献   

Tentative study of flow patterns in the North Aegean Sea using NOAA-AVHRR images and 2D model simulation     

Zodiatis  G.  Alexandri  S.  Pavlakis  P.  Jonsson  L.  Kallos  G.  Demetropoulos  A.  Georgiou  G.  Theodorou  A.  Balopoulos  E. 《Annales Geophysicae》1997,14(11):1221-1231

A statistical technique for image processing, the maximum cross correlation (MCC) method, was utilized on sequences of NOAA-AVHRR thermal data in order to explore the surface advective current dynamics at the discharge region of the Hellespont in the North Aegean Sea. A 2D numerical flow model was also used in order to simulate the barotropic flow pattern of the surface water layer. The model was forced with diurnal wind fields obtained for the same period as the satellite infrared images. The currents (magnitude and direction) derived from the two methods compare satisfactorily despite the fact that some model simplifications were made.  相似文献   

2D finite-difference elastic wave modelling including surface topography1     

Stig Hestholm  Bent Ruud 《Geophysical Prospecting》1994,42(5):371-390

A 2D numerical finite-difference algorithm accounting for surface topography is presented. Higher-order, dispersion-bounded, cost-optimized finite-difference operators are used in the interior of the numerical grid, while non-reflecting absorbing boundary conditions are used along the edges. Transformation from a curved to a rectangular grid achieves the modelling of the surface topography. We use free-surface boundary conditions along the surface. In order to obtain complete modelling of the effects of wave propagation, it is important to account for the surface topography, otherwise near-surface effects, such as scattering, are not modelled adequately. Even if other properties of the medium, for instance randomization, can improve numerical simulations, inclusion of the surface topography makes them more realistic.  相似文献   

Industrial minerals and rocks, aggregates and natural stones in the Nordic countries     

Kurt Johansson Rune B. Larsen Markku J. Lehtinen Lars Persson Mika Raeisaenen Stig A. Schack Pedersen Paer Weihed Nils-Gunnar Wik 《《幕》》2008,31(1):133-139

The Nordic countries, including Greenland, have a long tradition in mining. The industrial minerals sector is expanding in most Nordic countries and extensive development has taken place during the last few years. The main commodities mined are carbonate rocks, quartz, feldspar, apatite, olivine and talc.
A number of diferent types of dimension stones are quarried in all countries. Rock aggregates are increasingly important, replacing sand and gravel aggregate as construction materials in some countries due to the need to protect ground water supplies.  相似文献