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The primary goal of this study was to assess the effect of varying densities on serum reproductive parameters of immature rainbow trout Oncorhynchus mykiss. Experimental trout were maintained in intensive, pen-reared farms for 300 days in fresh water reservoirs. Initial densities were 4.6, 6.6, and 8.6 kg/m3 (40, 60, 80 ind./m3), indicated as SD1, SD2, SD3, and final densities were 31.1, 40.6, 49.3 kg/m3, respectively. A summary of the ovarian stages were observed by histological examination. Serum E2 (estradiol), T (testosterone) were evaluated by radioimmunoassay and FSH (follicle-stimulating-hormone), LH (luteinizing-hormone), vitellogenin, 17α,20β-P (17α,20βdihydroxy4-pregnen-3-one) were measured by enzyme-linked immunosorbent assay. Our findings demonstrated that ovarian development were retarded (from stage III to stage IV) at highest rearing density (SD3) after 180 days of intensive culture (over 40.6 kg/m3). In addition, we observed an inverse relationship between serum reproductive parameters and rearing density. Furthermore, compared to serum reproductive parameters of SD1, E2, T, FSH, vitellogenin, 17α,20β-P, GSI and LH of two higher density groups decreased firstly and significantly at 60 (over 15.9 kg/m 3 ), 180 (over 31.7 kg/m 3 ), 180 (over 40.6 kg/m3), 240 (over 36 kg/m3), 240 (over 36 kg/m3), 240 (over 45 kg/m3) and 300 (over 49.3 kg/m3) days, respectively. Comparing serum reproductive parameters within the same ovarian development stage of rainbow trout from varying densities revealed that higher population density also led to significantly lower overall serum reproductive parameters. Overall, this study presents the reproductive, endocrinological parameters of juvenile female rainbow trout at high rearing densities and indicates the need for rainbow trout (114.44±5.21 g, 19.69±0.31 cm) that are initially stocked at 6.6 or 8.6 kg/m3 should be classified and subdivided into lower density after 180 days of farming (not over 31.7 kg/m3). 相似文献
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本文研究急性温度胁迫对虹鳟血液生理和生化指标的影响,将虹鳟放入高温24℃和低温6℃水中热应激2h,然后放入16℃水中饲养,检测血液三类细胞数量及血清生化组分。结果表明:温度胁迫后,血液红细胞(wBC)和血红蛋白都呈现先降低后升高的趋势,低温组在12h降至最低,高温组在6h降至最低;白细胞在0h有不显著升高,其余时间与对照组没有显著差异;温度胁迫后,2个处理组血糖(GLU)在整个实验过程中都显著低于对照组;总蛋白(TP)和白蛋白(ALP)在温度胁迫后都呈现先降低后升高的趋势,低温组TP在6h达到最低值,ALP在12h达到最低值,高温组TP和ALP都在6h达到最低值;温度胁迫后,两处理组胆固醇(TC)都呈现先降低后升高再降低的趋势,在24h达到最大值;两处理组甘油三酯(TG)胁迫后都低于对照组,但没有明显变化趋势;温度胁迫后,无机物钙离子(Ca~(2+))只有高温组在24h显著降低,磷(P)只在胁迫后0h显著升高,其余时间两者没有明显变化。急性温度胁迫对虹鳟血细胞和血清生化组分产生了不同程度影响,为虹鳟运输、养殖条件优化等研究提供科学依据。 相似文献
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为开展松辽盆地深部长期观测、流体实验和探索白垩纪火山事件,利用松科二井东孔丰富、齐全的测井资料,对营城组火山岩岩性进行评价。通过测井响应特征分析发现,松科二井东孔营城组凝灰岩具有最强的放射性和导电性,高孔隙度的集块熔岩密度为低值,流纹岩表现出高密度和低导电性。利用测井交会图和成像识别模式,识别出松科二井东孔营城组火山岩以流纹岩、凝灰熔岩和集块熔岩为主,少量的凝灰岩。结合凝灰岩处测井曲线变化特点,证明了火山喷发间断的存在。流纹岩具有高碱、高Si、低Fe和低黏土矿物特征。T_2谱分析认为流纹岩有利于后期深部长期观测和流体实验的开展。研究成果对松科二井东孔后续火石岭组火成岩及整个松辽盆地火山岩研究具有一定的参考价值。 相似文献
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由中国科学院环境科学委员会和天津市环境保护局编辑的《天津-渤海湾地区环境遥感论文集》,已于1985年12月由科学出版社出版。 该文集综合反映了1980—1983年津渤环境遥感试验的主要科研成果。津渤环境遥感试验,是一项以城市环境为中心、以遥感为手段的多学科综合性研究课题,经过广大科技人员的共同努力,取得了丰硕的科研成果,解决或预示了天津城市发展与环境保护中的若干问题,为天津的城市建设、环境保护、 相似文献
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鞍山地质公司在棉花地区钻探施工中,遇到离地表100～300米的混合岩,裂隙发育,硅化程度也较高.采用双管人造金刚石钻头钻进时,岩芯堵塞严重,钻进效率很低,而用单管人造金刚石钻头钻进,则钻头寿命显著降低.1980年7月,鞍山地质公司与中南矿冶学院达成试验高效长寿的单管人造金刚石钻头的协议.协议要求:钻头寿命从原有的20米提高到30米;时效从原有的1.4米增加到1.8米.通过几个月的研究和试验,已完成并超过了所要求的指标.试验结果是:钻头平均寿命为41.1米,提高了105.5%,时效平均为2.04米,提高了45.7%. 相似文献
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