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The aim of this study was to examine whether a combination of biochemical, histopathological and toxicogenomic data could be used as a valuable tool for the assessment of biological risk associated with pollutants within the Tamar River and Estuary, S.W. England, U.K. Accordingly, biochemical and histopathological biomarkers (protein carbonyls, lipofuscin, neutral lipids, lysosomal stability [N-acetyl-β-hexosaminidase and neutral red], lysosomal volume, ferric reducing antioxidant power [FRAP] and malonaldehyde [MDA]) and gene expression profiles were assessed in 5 sites from the Tamar River and Estuary (Neal Point, Town Quay, Wilcove, Cremyll Ferry and Whitsand; and a reference site, Trebarwith Strand, N. Cornwall). PAHs were measured in mussel tissue and sediment and metals were measured in mussel tissue only. Data from the biomarkers was integrated into a Mussel Expert System (MES) model to produce a simple assessment of mussel stress. Clear gradients of mussel toxicity were identified by the biomarkers (with the exception of neutral lipids) with the highest impacted animals found furthest up the Tamar, whilst the MES was unable to identify a gradient of effect. Gene expression profiles also indicated a gradient of stress with the greatest number of significantly up- or down- regulated genes found at the uppermost 2 sites. The MES did, however, determine that mussels from all sites, except the reference site, were highly stressed; a conclusion that could not be inferred from the biomarker data alone. It is concluded that the MES is a valuable tool that permits integration and interpretation of complex sets of biomarker data by identifying the biological meaning of biomarker changes.  相似文献   
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Previous studies have shown that metal partitioned to a subcellular compartment containing trophically available metal (TAM) is readily available to predators and may be enhanced by increased binding of metal to heat-stable proteins (HSP - e.g., metallothioneins). The aim of the current investigation was to determine the influence of TAM on the trophic transfer of Cd along an experimental, three-level food chain: Artemia franciscana (brine shrimp)-->Palaemonetes pugio (grass shrimp)-->Fundulus heteroclitus (mummichog). P. pugio were fed for 7 days on A. franciscana exposed to Cd in solution (including (109)Cd as radiotracer) and subjected to subcellular fractionation or fed to F. heteroclitus. An HSP-driven increase in the percentage of Cd associated with TAM (TAM-Cd%) in A. franciscana exposed to 1 muM Cd resulted in a bioenhancement (i.e., a greater than linear increase with respect to A. franciscana exposure) of Cd trophic transfer to P. pugio. Increased dietary Cd exposure did not affect TAM-Cd% in P. pugio nor trophic transfer to F. heteroclitus.  相似文献   
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The aim of the current work was to determine over 10 days the effects of water-borne exposure of the marine amphipod Gammarus locusta to 4 microgCu l(-1) on the metallothionein (MT; measured by differential pulse polarography) protection system and lipid peroxidation (LP: thiobarbituric acid-reactive malondialdehyde equivalents) as a measure of oxidative damage. MT levels in exposed animals increased significantly at day 2 (36% > control; P < 0.001) and remained high at days 6 and 10 (55 and 38%, respectively, P < 0.001). The maximum level of MT at day 6 coincided with the highest Cu body-burden. LP increased within I day of exposure, indicative of Cu as an oxidative stressor. However, in contrast to MT, the highest LP level was seen at day 4 (68% > control, P < 0.001) before returning to control values by day 6, indicating a protective role of MT against the pro-oxidant effects of Cu.  相似文献   
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The Conference,organized by Dutch and Bel-gian chemometricians,was held in the FlemishCultural Centre in Amsterdam.This smalltheatre,with its dark red interior and stage-properties placed arbitrarily over the smallpresentation platform,appeared to be an inspir-ing place for the participants.A large number of  相似文献   
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The San Francisco Estuary Regional Monitoring Program initiated surveillance monitoring to identify previously unmonitored synthetic organic contaminants in the San Francisco Estuary. Organic extracts of water samples were analyzed using gas chromatography-mass spectrometry in full scan mode. The major contaminant classes identified in the samples were fire retardants, pesticides, personal care product ingredients, and plasticizers. Evidence from the literature suggests that some of these contaminants can persist in the environment, induce toxicity, and accumulate in marine biota and in higher food chain consumers. The major sources of these contaminants into the marine environment are the discharge of municipal and industrial wastewater effluents, urban stormwater, and agricultural runoff. As a proactive effort, it is suggested that surveillance studies be used routinely in monitoring programs to identify and prevent potential problem contaminants from harming the marine environment.  相似文献   
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在工程测量中,经常需要解算各种形状的物体体积。本文介绍了一种求解体积的数值算法,即用一个数学单元近似表述物体的形状,然后再通过数值积分解算体积。定义数学单元时,要求它们必须经过物体表面边界上预先定义的8个或者20个结点。实现该算法的计算机程序只需要一个简单的数据文件,其中包括结点坐标和结点个数。文中还给出了几个典型问题的解算结果,其精度可精确到3%,较好地满足了限差要求。  相似文献   
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通过多变(相关分析)和趋势分析等统计方法对宏观调查数据进行了解释.这种方法使我们通过评定不同宏观效应叠加的程度、估计调查数据的不确定性更客观地评定烈度.通过滤波确定烈度场的区域分量,使我们消除观测中的局部变化.给出了选取适当滤波参数的准则.本文应用这一方法对发生在意大利的一次地震的调查数据进行了处理.  相似文献   
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本文主要介绍一种与射线追踪无关的走时成像方法,使用一种非线性最优化算法,称为非常快速的仿真退火。利用立方B样条对地下地质体进行参数化,此网格在水平和垂直坐标是可变化的,这个灵活的网格可以容 等复杂程度的构造,并只用几个节点,我们试图求观测与预测走时之间的均言差进行极小化。时使用有限差分算法,不仅在样条节点位置上求速度,而且还求在每个维方向上的网格间距,由于在计算期间避免了局部极小,求出的解与初始模  相似文献   
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