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1.
Anthropogenic emission of atmospheric carbon dioxide(CO_2) has led to a rapid increase in atmospheric CO_2 concentration. Increasing atmospheric CO_2 can reduce seawater pH and carbonate ions, which may adversely affect the survival of the larvae of calcareous animals. Cyclina sinensis is a commercially and ecologically important species in several Asian countries. Living in coast shallow waters, this species has experienced the coastal environmental changes frequently throughout its life cycle. In this study, we simulated possible future seawater pH values including 8.2, 7.8 and 7.4 and examined the effects of ocean acidification on the early development of C. sinensis. Clam embryos were incubated for 48 h(2 d) in control and high-CO_2 seawater to compare embryogenesis, larval growth and swimming behavior. Fertilization rate was quite sensitive to pH, and moderate acidification could induce a significant decrease in fertilization rate. However, only extreme acidification could bring significant negative effect to hatching rate, body size, and average path velocity of trochophora. Moreover, with seawater acidification, C. sinensis needs much more time to reach the same developmental stage, which increases the risk of larva survival. Together with recent studies demonstrating negative impacts of high CO_2 on fertilization and larva swimming behavior, the results imply a future decrease of C. sinensis populations in oceans if its acclimation to the predicted environmental alteration does not occur. 相似文献
2.
为充分利用先进设备和技术,提高我国海域使用动态监管水平,文章介绍无人机全景技术及其作业流程,并分析大笔架山海域无人机全景监视监测实例。研究结果表明:目前全景图主要利用普通相机拍摄的照片经专业图像软件后期处理生成,在多个行业领域应用,结合无人机的利用可大大扩展视野,具有快速高效、安全可靠和操作简便等优势,但在测量、自动化和视距等方面仍存在局限性;无人机全景图作业主要包括方案制订、数据获取、数据处理和图像发布4个步骤;海域无人机全景监视监测工作的开展为大笔架山海域使用动态监管提供有力的数据支撑。 相似文献
3.
创伤弧菌是一种可感染人类的河口病原菌。建立快速，特异而敏感的检测方法，有助于创伤弧菌感染的早期疾病诊断和及时治疗。本研究设计了针对vvhA基因的一系列引物（包括两对外部引物和两对内部引物），采用环介导等温扩增技术（LAMP）来检测创伤弧菌。结果显示，本方法的最适扩增温度是63℃，反应仅需35分钟。扩增产物不仅可以用含有DNA ladder的琼脂糖凝胶电泳检出，也可借助钙黄绿素直接肉眼观察。采用45株菌株检测该方法的特异性，其中所有创伤弧菌均被检出而其他菌株检测结果皆为阴性。本方法的敏感性是普通PCR扩增的100倍，同时利用该方法可以准确检测出所有的模拟样品、临床标本及环境样品。与其他已知方法比较，针对vvhA基因的介导等温扩增技术可以快速、简单、敏感及特异地鉴定创伤弧菌。 相似文献
4.
牟平-乳山成矿带是山东省重要的金成矿带,姜格庄金矿位于牟平-乳山成矿带东北部,米山断裂西侧。姜格庄金矿矿床成因为中低温岩浆热液金矿床,控矿构造以近SN向断裂构造为主。矿区内地表出露矿化蚀变带5个,圈定矿体5个,矿体多呈脉状、透镜状,成矿严格受矿断裂控制,产状与蚀变带一致,围岩为邱家单元片麻状黑云二长花岗岩,蚀变主要为硅化、钾化、褐铁矿化等。矿石矿物主要有黄铁矿、褐铁矿及黄铜矿,金矿物主要为自然金等。通过对矿体地质特征和分布规律研究,认为该区域断裂构造内具有较好的找矿潜力。 相似文献
5.
综述了我国海域无脊椎动物的分类学和系统演化研究的历史和概况，以及我国分类系统学工作者在海洋无脊椎动物分类学、区系与动物地理学、系统发育与分子系统学领域的主要工作，重点介绍了中国科学院海洋研究所的海洋无脊椎动物分类学工作。涉及类群包括原生动物、海绵动物、刺胞动物、线虫、多毛类环节动物、星虫、螠虫、软体动物、节肢动物、苔藓动物、毛颚动物、棘皮动物、半索动物等主要的无脊椎动物门类。涉及海域以我国管辖海域，特别是中国近海为主，也涉及了西太平洋、西南印度洋等深海环境的无脊椎动物类群的分类学报道。本文总结过去，展望未来，对于在我国在海洋无脊椎动物分类与系统演化研究领域成就基础上，发现薄弱环节，研讨今后本学科的发展方向，填补研究空白，赶超本领域国际前沿，都有重要借鉴意义。 相似文献
6.
Biofloc technology has been applied successfully in the intensive aquaculture of several fish and shrimp species.The growth of heterotrophic microorganisms can be stimulated through adding extra carbon,which reduces the nitrogen level in the water and provides microbial protein to the animals.However,most of the studies and practical applications have been conducted in freshwater and marine environment.This paper focused on brine shrimp Artemia that lives in high salinity environment together with other halophilic or halotolerant microorganisms.The effect of carbon supplementation on Artemia growth,water quality,and microbial diversity of bioflocs was studied in the closed culture condition without any water exchange.The salinity of the culture medium was 100.A 24-d culture trial was conducted through supplementing sucrose at carbon/nitrogen(C/N)ratio of 5,15,and 30(Su5,Sul5,and Su30),respectively.The culture without adding sucrose was used as a control.Artemia was fed formulated feed at a feeding ration of 60% recommended feeding level.The results showed that sucrose supplementation at higher C/N ratio(15 and 30)significantly improved the Artemia survival,growth and water quality(P0.05).Addition of sucrose at C/N ratio of 15 and 30 significantly increased biofloc volume(BFV)(P0.05).The Illumina MiSeq sequencing analysis showed that supplementing carbon at C/N ratio of 15 had a better total bacterial diversity and richness,and shaped the microbial composition at genera level.This study should provide information for studying the mechanism of biofloc technology and its application in high salinity culture conditions. 相似文献
7.
Mariculture has rapidly grown worldwide, which might greatly influence the biogeochemical cycle of organic carbon in coastal seas. In this study, several geochemical parameters, including grain size composition, sedimentary total organic carbon(TOC), total nitrogen(TN), stable carbon(δ13 C) and nitrogen(δ15 N) isotopic compositions, were analyzed for surface sediments collected from different mariculture zones of Sanggou Bay and in different seasons. We investigated the composition and distribution of organic matter in surface sediments and further evaluated the contribution of mariculture activities to TOC sources. The TOC and TN contents(mass percentage) in the bay were in the range of 0.14% to 1.45% and 0.03% to 0.20%, respectively. The spatial distribution indicated that sedimentary TOC and TN contents in shellfish monoculture and shellfish-kelp polyculture zones were higher than in other mariculture zones, which might be related to grain size composition and mariculture organisms. Seasonal variations of TOC contents were observed in different mariculture zones. The TOC/TN atomic ratio(C/N), δ13 C and δ15 N were in the ranges of 5.97 to 10.97,-21.76‰ to-13.14‰ and 2.13‰ to 8.08‰, respectively, implying that sedimentary organic matter in Sanggou Bay was the mixture of marine phytoplankton, terrestrial and maricultural sources. A simple mixing model based on δ13 C was applied and the results indicated that the relative contributions of organic carbon sources in Sanggou Bay followed the order kelp(36.6%) ? marine phytoplankton(28.7%) ? shellfish bio-deposition(23.8%) ? terrestrial input(10.9%). Surface sediments in Sanggou Bay were dominated by mariculture-derived organic carbon, which on average accounted for 60.4% of TOC. 相似文献
8.
9.
钢筋混凝土（RC）板与一定厚度的土颗粒缓冲层组合结构被广泛用于山区高位单体及群发性崩塌落石的防治,为研究此类防护结构在落石作用下的冲击力衰减规律及RC板的破坏模式,开展了室外系列落石冲击试验。结果表明,增大缓冲层厚度能够有效减小最大冲击力,峰值加速度随缓冲层厚度减小而增大,尤其在缓冲层厚度为0.1 m及0.2 m时,最大值急剧增大,峰值加速度与缓冲层厚度的变化满足指数函数关系;根据量纲分析原理得到缓冲层最大冲击深度与动能的平方成正比、与最大入射冲击力成反比的计算公式,且与实测值较吻合;入射冲击力在缓冲层内的衰减率随缓冲层厚度的增加以指数函数递增,在0.6 m缓冲层厚度下可使峰值冲击力衰减70%左右;随累积冲击能级的增大,RC板经历了弯曲起裂及扩展、次级弯曲裂纹和剪裂纹产生及跨中弯曲裂纹贯通的过程,试验结束时RC板整体表现出典型的弯曲破坏特征。 相似文献
10.